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Home / Equine Research Bank / Life (Basel) / The Inhibitory Effect of Noscapine on the In Vitro Cathepsin...
Life (Basel, Switzerland)2021; 11(10); 1107; doi: 10.3390/life11101107

The Inhibitory Effect of Noscapine on the In Vitro Cathepsin G-Induced Collagen Expression in Equine Endometrium.

Abstract: Cathepsin G (CAT) is a protease released by neutrophils when forming neutrophil extracellular traps that was already associated with inducing type I collagen (COL1) in equine endometrium in vitro. Endometrosis is a fibrotic condition mainly characterized by COL1 deposition in the equine endometrium. The objective was to evaluate if noscapine (an alkaloid for cough treatment with anti-neoplastic and anti-fibrotic properties) would reduce transcription (evaluated by qPCR) and COL1 protein relative abundance (evaluated by western blot) induced by CAT in equine endometrial explants from follicular and mid-luteal phases treated for 24 or 48 h. The explants treated with CAT increased COL1 expression. Noscapine decreased transcription at both estrous cycle phases, but COL1 relative protein only at the follicular phase, both induced by CAT. Additionally, the noscapine anti-fibrotic action was found to be more effective in the follicular phase. The CAT treatment caused more fibrosis at the longest period of treatment, while noscapine acted better at the shortest time of treatment. Our results showed that noscapine could act as an anti-fibrotic drug in equine endometrosis by inhibiting CAT in vitro. Noscapine offers a new promising therapeutic tool for treating fibrosis as a single non-selective agent to be considered in the future.
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Publication Date: 2021-10-19 PubMed ID: 34685478PubMed Central: PMC8539599DOI: 10.3390/life11101107Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research is about the anti-fibrotic impact of noscapine, a cough treatment alkaloid, on equine endometrosis by inhibiting the action of cathepsin G, a protease associated with inducing type I collagen, in vitro.

Objective and Background

  • The primary objective of this study was to determine whether noscapine, an alkaloid primarily used in cough treatment, can reduce the transcription and abundance of type I collagen (COL1) protein induced by Cathepsin G (CAT) in horse endometrial explants. This investigation is significant as CAT is a protease released by neutrophils, known for inducing COL1 in the equine endometrium.
  • Endometrosis in horses is a fibrotic condition mainly characterized by the deposition of COL1 in the endometrium. Consequentially, any substance capable of reducing COL1 levels could potentially serve as an anti-fibrotic drug in equine endometrosis.

Methodology and Findings

  • The research used equine endometrial explants from the follicular and mid-luteal phases, treating them for 24 or 48 hours. When these explants were treated with CAT, it increased the expression of COL1.
  • However, when treated with noscapine, the transcription was observed to reduce in both estrous cycle phases. In contrast, the relative protein of COL1 dropped only during the follicular phase.
  • Additionally, it was found that the anti-fibrotic action of noscapine was more effective during the follicular phase.
  • On examining the duration of the treatment, more fibrosis was identified when treated with CAT for an extended period while noscapine was found to be most effective when administered for a shorter duration.

Conclusion

  • The results clearly demonstrated that noscapine can function as an anti-fibrotic agent for equine endometrosis by inhibiting the action of CAT in vitro. As such, it presents a promising therapeutic tool for treating fibrosis.
  • Moreover, it can be employed as a single non-selective agent for future treatments, thus broadening the scope of its application and indicating a positive outlook in controlling and managing fibrotic conditions, not just in equine endometrosis but potentially in other areas as well.

Cite This Article

APA
Amaral A, Fernandes C, Szóstek-Mioduchowska A, Lukasik K, Rebordão MR, Pinto-Bravo P, Skarzynski DJ, Ferreira-Dias G. (2021). The Inhibitory Effect of Noscapine on the In Vitro Cathepsin G-Induced Collagen Expression in Equine Endometrium. Life (Basel), 11(10), 1107. https://doi.org/10.3390/life11101107

Publication

Life (Basel, Switzerland)
ISSN: 2075-1729
NlmUniqueID: 101580444
Country: Switzerland
Language: English
Volume: 11
Issue: 10
PII: 1107

Researcher Affiliations

Amaral, Ana
  • CIISA-Centro de Investigação Interdisciplinar em Sanidade Animal, Departamento de Morfologia e Função, Faculdade de Medicina Veterinária, Universidade de Lisboa, 1300-477 Lisboa, Portugal.
Fernandes, Carina
  • CIISA-Centro de Investigação Interdisciplinar em Sanidade Animal, Departamento de Morfologia e Função, Faculdade de Medicina Veterinária, Universidade de Lisboa, 1300-477 Lisboa, Portugal.
Szóstek-Mioduchowska, Anna
  • Institute of Animal Reproduction and Food Research, Polish Academy of Science, 10-748 Olsztyn, Poland.
Lukasik, Karolina
  • Institute of Animal Reproduction and Food Research, Polish Academy of Science, 10-748 Olsztyn, Poland.
Rebordão, Maria Rosa
  • CIISA-Centro de Investigação Interdisciplinar em Sanidade Animal, Departamento de Morfologia e Função, Faculdade de Medicina Veterinária, Universidade de Lisboa, 1300-477 Lisboa, Portugal.
  • Polytechnic of Coimbra, Coimbra Agriculture School, Bencanta, 3045-601 Coimbra, Portugal.
Pinto-Bravo, Pedro
  • Polytechnic of Coimbra, Coimbra Agriculture School, Bencanta, 3045-601 Coimbra, Portugal.
Skarzynski, Dariusz Jan
  • Institute of Animal Reproduction and Food Research, Polish Academy of Science, 10-748 Olsztyn, Poland.
Ferreira-Dias, Graça
  • CIISA-Centro de Investigação Interdisciplinar em Sanidade Animal, Departamento de Morfologia e Função, Faculdade de Medicina Veterinária, Universidade de Lisboa, 1300-477 Lisboa, Portugal.

Grant Funding

  • PPN/BIL/2018/1/00250/U/0001 / Narodowa Agencja Wymiany Akademickiej
  • UIDB/00276/2020 / Fundau00e7u00e3o para a Ciu00eancia e Tecnologia
  • PTDC/CVT-REP/4202/2014 / Fundau00e7u00e3o para a Ciu00eancia e Tecnologia
  • SFRH/BD/101058/2014 / Fundau00e7u00e3o para a Ciu00eancia e Tecnologia

Conflict of Interest Statement

The authors declare no conflict of interest.

References

This article includes 74 references
  1. Brinkmann V, Reichard U, Goosmann C, Fauler B, Uhlemann Y, Weiss DS, Weinrauch Y, Zychlinsky A. Neutrophil extracellular traps kill bacteria.. Science 2004 Mar 5;303(5663):1532-5.
    doi: 10.1126/science.1092385pubmed: 15001782google scholar: lookup
  2. Averhoff P, Kolbe M, Zychlinsky A, Weinrauch Y. Single residue determines the specificity of neutrophil elastase for Shigella virulence factors.. J Mol Biol 2008 Apr 4;377(4):1053-66.
    doi: 10.1016/j.jmb.2007.12.034pubmed: 18295791google scholar: lookup
  3. Guo J, Tu J, Hu Y, Song G, Yin Z. Cathepsin G cleaves and activates IL-36γ and promotes the inflammation of psoriasis.. Drug Des Devel Ther 2019;13:581-588.
    doi: 10.2147/DDDT.S194765pmc: PMC6372007pubmed: 30804664google scholar: lookup
  4. Herrmann SM, Funke-Kaiser H, Schmidt-Petersen K, Nicaud V, Gautier-Bertrand M, Evans A, Kee F, Arveiler D, Morrison C, Orzechowski HD, Elbaz A, Amarenco P, Cambien F, Paul M. Characterization of polymorphic structure of cathepsin G gene: role in cardiovascular and cerebrovascular diseases.. Arterioscler Thromb Vasc Biol 2001 Sep;21(9):1538-43.
    doi: 10.1161/hq0901.095555pubmed: 11557685google scholar: lookup
  5. Bhojak TJ, DeKosky ST, Ganguli M, Kamboh MI. Genetic polymorphism in the cathepsin G gene and the risk of Alzheimer's disease.. Neurosci Lett 2001 Aug 24;309(2):138-40.
    doi: 10.1016/S0304-3940(01)02033-Xpubmed: 11502364google scholar: lookup
  6. Pérez-Is L, Ocaña MG, Montes AH, Carton JA, Álvarez V, Meana Á, Fierer J, Valle-Garay E, Asensi V. The N125S polymorphism in the cathepsin G gene (rs45567233) is associated with susceptibility to osteomyelitis in a Spanish population.. PLoS One 2019;14(10):e0220022.
    doi: 10.1371/journal.pone.0220022pmc: PMC6812796pubmed: 31647805google scholar: lookup
  7. Brehm A, Geraghty P, Campos M, Garcia-Arcos I, Dabo AJ, Gaffney A, Eden E, Jiang XC, D'Armiento J, Foronjy R. Cathepsin G degradation of phospholipid transfer protein (PLTP) augments pulmonary inflammation.. FASEB J 2014 May;28(5):2318-31.
    doi: 10.1096/fj.13-246843pmc: PMC3986847pubmed: 24532668google scholar: lookup
  8. Shimoda N, Fukazawa N, Nonomura K, Fairchild RL. Cathepsin g is required for sustained inflammation and tissue injury after reperfusion of ischemic kidneys.. Am J Pathol 2007 Mar;170(3):930-40.
    doi: 10.2353/ajpath.2007.060486pmc: PMC1864870pubmed: 17322378google scholar: lookup
  9. Twigg MS, Brockbank S, Lowry P, FitzGerald SP, Taggart C, Weldon S. The Role of Serine Proteases and Antiproteases in the Cystic Fibrosis Lung.. Mediators Inflamm 2015;2015:293053.
    doi: 10.1155/2015/293053pmc: PMC4491392pubmed: 26185359google scholar: lookup
  10. Guerra M, Frey D, Hagner M, Dittrich S, Paulsen M, Mall MA, Schultz C. Cathepsin G Activity as a New Marker for Detecting Airway Inflammation by Microscopy and Flow Cytometry.. ACS Cent Sci 2019 Mar 27;5(3):539-548.
    doi: 10.1021/acscentsci.8b00933pmc: PMC6439450pubmed: 30937381google scholar: lookup
  11. Gudmann NS, Manon-Jensen T, Sand JMB, Diefenbach C, Sun S, Danielsen A, Karsdal MA, Leeming DJ. Lung tissue destruction by proteinase 3 and cathepsin G mediated elastin degradation is elevated in chronic obstructive pulmonary disease.. Biochem Biophys Res Commun 2018 Sep 10;503(3):1284-1290.
    doi: 10.1016/j.bbrc.2018.07.038pubmed: 30017196google scholar: lookup
  12. Tan K, Brasch HD, van Schaijik B, Armstrong JR, Marsh RW, Davis PF, Tan ST, Itinteang T. Expression and Localization of Cathepsins B, D, and G in Dupuytren's Disease.. Plast Reconstr Surg Glob Open 2018 Feb;6(2):e1686.
    doi: 10.1097/GOX.0000000000001686pmc: PMC5865920pubmed: 29616179google scholar: lookup
  13. Rebordão MR, Amaral A, Lukasik K, Szóstek-Mioduchowska A, Pinto-Bravo P, Galvão A, Skarzynski DJ, Ferreira-Dias G. Constituents of neutrophil extracellular traps induce in vitro collagen formation in mare endometrium.. Theriogenology 2018 Jun;113:8-18.
    doi: 10.1016/j.theriogenology.2018.02.001pubmed: 29452855google scholar: lookup
  14. Amaral A, Fernandes C, Lukasik K, Szóstek-Mioduchowska A, Baclawska A, Rebordão MR, Aguiar-Silva J, Pinto-Bravo P, Skarzynski DJ, Ferreira-Dias G. Elastase inhibition affects collagen transcription and prostaglandin secretion in mare endometrium during the estrous cycle.. Reprod Domest Anim 2018 Sep;53 Suppl 2:66-69.
    doi: 10.1111/rda.13258pubmed: 30238664google scholar: lookup
  15. Amaral A, Fernandes C, Rebordão MR, Szóstek-Mioduchowska A, Lukasik K, Gawronska-Kozak B, Telo da Gama L, Skarzynski DJ, Ferreira-Dias G. The In Vitro Inhibitory Effect of Sivelestat on Elastase Induced Collagen and Metallopeptidase Expression in Equine Endometrium.. Animals (Basel) 2020 May 16;10(5).
    doi: 10.3390/ani10050863pmc: PMC7278485pubmed: 32429399google scholar: lookup
  16. Amaral A, Fernandes C, Morazzo S, Rebordão MR, Szóstek-Mioduchowska A, Lukasik K, Gawronska-Kozak B, Telo da Gama L, Skarzynski DJ, Ferreira-Dias G. The Inhibition of Cathepsin G on Endometrial Explants With Endometrosis in the Mare.. Front Vet Sci 2020;7:582211.
    doi: 10.3389/fvets.2020.582211pmc: PMC7661753pubmed: 33195599google scholar: lookup
  17. Amaral A, Fernandes C, Rebordão MR, Szóstek-Mioduchowska A, Lukasik K, Pinto-Bravo P, Telo da Gama L, Jan Skarzynski D, Ferreira-Dias G. Myeloperoxidase Inhibition Decreases the Expression of Collagen and Metallopeptidase in Mare Endometria under In Vitro Conditions.. Animals (Basel) 2021 Jan 16;11(1).
    doi: 10.3390/ani11010208pmc: PMC7830995pubmed: 33467081google scholar: lookup
  18. Katila T. Onset and Duration of Uterine Inflammatory Response of Mares after Insemination with Fresh Semen. Biol. Reprod. 1995;52:515–517.
    doi: 10.1093/biolreprod/52.monograph_series1.515google scholar: lookup
  19. Christoffersen M, Woodward E, Bojesen AM, Jacobsen S, Petersen MR, Troedsson MH, Lehn-Jensen H. Inflammatory responses to induced infectious endometritis in mares resistant or susceptible to persistent endometritis.. BMC Vet Res 2012 Mar 29;8:41.
    doi: 10.1186/1746-6148-8-41pmc: PMC3368729pubmed: 22458733google scholar: lookup
  20. Christoffersen M, Troedsson M. Inflammation and fertility in the mare.. Reprod Domest Anim 2017 Aug;52 Suppl 3:14-20.
    doi: 10.1111/rda.13013pubmed: 28815848google scholar: lookup
  21. Canisso IF, Segabinazzi LGTM, Fedorka CE. Persistent Breeding-Induced Endometritis in Mares - a Multifaceted Challenge: From Clinical Aspects to Immunopathogenesis and Pathobiology.. Int J Mol Sci 2020 Feb 20;21(4).
    doi: 10.3390/ijms21041432pmc: PMC7073041pubmed: 32093296google scholar: lookup
  22. Troedsson MH, Liu IK, Thurmond M. Function of uterine and blood-derived polymorphonuclear neutrophils in mares susceptible and resistant to chronic uterine infection: phagocytosis and chemotaxis.. Biol Reprod 1993 Sep;49(3):507-14.
    doi: 10.1095/biolreprod49.3.507pubmed: 8399843google scholar: lookup
  23. LeBlanc MM, Neuwirth L, Asbury AC, Tran T, Mauragis D, Klapstein E. Scintigraphic measurement of uterine clearance in normal mares and mares with recurrent endometritis.. Equine Vet J 1994 Mar;26(2):109-13.
    doi: 10.1111/j.2042-3306.1994.tb04346.xpubmed: 8575370google scholar: lookup
  24. Rebordão MR, Amaral A, Fernandes C, Silva E, Lukasik K, Szóstek-Mioduchowska A, Pinto-Bravo P, Galvão A, Skarzynski DJ, Ferreira-Dias G. Enzymes Present in Neutrophil Extracellular Traps May Stimulate the Fibrogenic PGF(2α) Pathway in the Mare Endometrium.. Animals (Basel) 2021 Sep 6;11(9).
    doi: 10.3390/ani11092615pmc: PMC8469524pubmed: 34573581google scholar: lookup
  25. Kenney RM. Cyclic and pathologic changes of the mare endometrium as detected by biopsy, with a note on early embryonic death.. J Am Vet Med Assoc 1978 Feb 1;172(3):241-62.
    pubmed: 621166
  26. Hoffmann C, Ellenberger C, Mattos RC, Aupperle H, Dhein S, Stief B, Schoon HA. The equine endometrosis: new insights into the pathogenesis.. Anim Reprod Sci 2009 Apr;111(2-4):261-78.
    doi: 10.1016/j.anireprosci.2008.03.019pubmed: 18468817google scholar: lookup
  27. Lehmann J, Ellenberger C, Hoffmann C, Bazer FW, Klug J, Allen WR, Sieme H, Schoon HA. Morpho-functional studies regarding the fertility prognosis of mares suffering from equine endometrosis.. Theriogenology 2011 Oct 15;76(7):1326-36.
    doi: 10.1016/j.theriogenology.2011.06.001pubmed: 21855986google scholar: lookup
  28. Ley WB, Bowen JM, Sponenberg DP, Lessard PN. Dimethyl sulfoxide intrauterine therapy in the mare: effects upon endometrial histological features and biopsy classification.. Theriogenology 1989 Aug;32(2):263-76.
    doi: 10.1016/0093-691X(89)90317-8pubmed: 16726673google scholar: lookup
  29. Buczkowska J, Kozdrowski R, Nowak M, Raś A, Mrowiec J. Endometrosis--significance for horse reproduction, pathogenesis, diagnosis, and proposed therapeutic methods.. Pol J Vet Sci 2014;17(3):547-54.
    doi: 10.2478/pjvs-2014-0083pubmed: 25286671google scholar: lookup
  30. Podico G, Canisso IF, Roady PJ, Austin SM, Carossino M, Balasuriya U, Ellerbrock RE, Lima FS, Ferreira-Dias G, Douglas RH. Uterine responses and equine chorionic gonadotropin concentrations after two intrauterine infusions with kerosene post early fetal loss in mares.. Theriogenology 2020 Apr 15;147:202-210.
    doi: 10.1016/j.theriogenology.2019.11.014pubmed: 31787468google scholar: lookup
  31. Navarrete F, Saravia F, Cisterna G, Rojas F, Silva PP, Rodríguez-Alvarez L, Rojas D, Cabezas J, Mançanares ACF, Castro FO. Assessment of the anti-inflammatory and engraftment potential of horse endometrial and adipose mesenchymal stem cells in an in vivo model of post breeding induced endometritis.. Theriogenology 2020 Oct 1;155:33-42.
    doi: 10.1016/j.theriogenology.2020.06.010pubmed: 32622203google scholar: lookup
  32. Cabezas J, Rojas D, Wong Y, Telleria F, Manriquez J, Mançanares ACF, Rodriguez-Alvarez LL, Castro FO. In vitro preconditioning of equine adipose mesenchymal stem cells with prostaglandin E(2), substance P and their combination changes the cellular protein secretomics and improves their immunomodulatory competence without compromising stemness.. Vet Immunol Immunopathol 2020 Oct;228:110100.
    doi: 10.1016/j.vetimm.2020.110100pubmed: 32871408google scholar: lookup
  33. Karlsson MO, Dahlström B, Eckernäs SA, Johansson M, Alm AT. Pharmacokinetics of oral noscapine.. Eur J Clin Pharmacol 1990;39(3):275-9.
    doi: 10.1007/BF00315110pubmed: 2257866google scholar: lookup
  34. Rida PC, LiVecche D, Ogden A, Zhou J, Aneja R. The Noscapine Chronicle: A Pharmaco-Historic Biography of the Opiate Alkaloid Family and its Clinical Applications.. Med Res Rev 2015 Sep;35(5):1072-96.
    doi: 10.1002/med.21357pmc: PMC4778555pubmed: 26179481google scholar: lookup
  35. Esnaashari SS, Muhammadnejad S, Amanpour S, Amani A. A Combinational Approach Towards Treatment of Breast Cancer: an Analysis of Noscapine-Loaded Polymeric Nanoparticles and Doxorubicin.. AAPS PharmSciTech 2020 Jun 5;21(5):166.
    doi: 10.1208/s12249-020-01710-3pubmed: 32504144google scholar: lookup
  36. Ke Y, Ye K, Grossniklaus HE, Archer DR, Joshi HC, Kapp JA. Noscapine inhibits tumor growth with little toxicity to normal tissues or inhibition of immune responses.. Cancer Immunol Immunother 2000 Jul;49(4-5):217-25.
    doi: 10.1007/s002620000109pubmed: 10941904google scholar: lookup
  37. Aneja R, Dhiman N, Idnani J, Awasthi A, Arora SK, Chandra R, Joshi HC. Preclinical pharmacokinetics and bioavailability of noscapine, a tubulin-binding anticancer agent.. Cancer Chemother Pharmacol 2007 Nov;60(6):831-9.
    doi: 10.1007/s00280-007-0430-ypubmed: 17285314google scholar: lookup
  38. Newcomb EW, Lukyanov Y, Smirnova I, Schnee T, Zagzag D. Noscapine induces apoptosis in human glioma cells by an apoptosis-inducing factor-dependent pathway.. Anticancer Drugs 2008 Jul;19(6):553-63.
    doi: 10.1097/CAD.0b013e3282ffd68dpubmed: 18525314google scholar: lookup
  39. Liu M, Luo XJ, Liao F, Lei XF, Dong WG. Noscapine induces mitochondria-mediated apoptosis in gastric cancer cells in vitro and in vivo.. Cancer Chemother Pharmacol 2011 Mar;67(3):605-12.
    doi: 10.1007/s00280-010-1356-3pubmed: 20490799google scholar: lookup
  40. Yang ZR, Liu M, Peng XL, Lei XF, Zhang JX, Dong WG. Noscapine induces mitochondria-mediated apoptosis in human colon cancer cells in vivo and in vitro.. Biochem Biophys Res Commun 2012 May 11;421(3):627-33.
    doi: 10.1016/j.bbrc.2012.04.079pubmed: 22546556google scholar: lookup
  41. Tian X, Liu M, Huang X, Zhu Q, Liu W, Chen W, Zou Y, Cai Y, Huang S, Chen A, Zhan T, Huang M, Chen X, Han Z, Tan J. Noscapine Induces Apoptosis in Human Colon Cancer Cells by Regulating Mitochondrial Damage and Warburg Effect via PTEN/PI3K/mTOR Signaling Pathway.. Onco Targets Ther 2020;13:5419-5428.
    doi: 10.2147/OTT.S232137pmc: PMC7297602pubmed: 32606759google scholar: lookup
  42. Idänpään-Heikkilä JE. Studies on the fate of 3H-noscapine in mice and rats.. Ann Med Exp Biol Fenn 1968;46(2):201-16.
    pubmed: 4896713
  43. LASAGNA L, OWENS AH Jr, SHNIDER BI, GOLD GL. Toxicity after large doses of noscapine.. Cancer Chemother Rep 1961 Dec;15:33-4.
    pubmed: 14462566
  44. Sebak S, Mirzaei M, Malhotra M, Kulamarva A, Prakash S. Human serum albumin nanoparticles as an efficient noscapine drug delivery system for potential use in breast cancer: preparation and in vitro analysis.. Int J Nanomedicine 2010 Sep 20;5:525-32.
    doi: 10.2147/ijn.s10443pmc: PMC2948945pubmed: 20957217google scholar: lookup
  45. Kach J, Sandbo N, La J, Denner D, Reed EB, Akimova O, Koltsova S, Orlov SN, Dulin NO. Antifibrotic effects of noscapine through activation of prostaglandin E2 receptors and protein kinase A.. J Biol Chem 2014 Mar 14;289(11):7505-13.
    doi: 10.1074/jbc.M113.546812pmc: PMC3953264pubmed: 24492608google scholar: lookup
  46. Doddapaneni R, Patel K, Chowdhury N, Singh M. Noscapine chemosensitization enhances docetaxel anticancer activity and nanocarrier uptake in triple negative breast cancer.. Exp Cell Res 2016 Aug 1;346(1):65-73.
    doi: 10.1016/j.yexcr.2016.05.006pmc: PMC5117688pubmed: 27177833google scholar: lookup
  47. Amaral A, Fernandes C, Szóstek-Mioduchowska A, Rebordão MR, Skarzynski DJ, Ferreira-Dias G. Noscapine Acts as a Protease Inhibitor of In Vitro Elastase-Induced Collagen Deposition in Equine Endometrium.. Int J Mol Sci 2021 May 19;22(10).
    doi: 10.3390/ijms22105333pmc: PMC8159119pubmed: 34069423google scholar: lookup
  48. Roberto da Costa RP, Ferreira-Dias G, Mateus L, Korzekwa A, Andronowska A, Platek R, Skarzynski DJ. Endometrial nitric oxide production and nitric oxide synthases in the equine endometrium: Relationship with microvascular density during the estrous cycle.. Domest Anim Endocrinol 2007 May;32(4):287-302.
    doi: 10.1016/j.domaniend.2006.03.007pubmed: 16647832google scholar: lookup
  49. Rebordão MR, Amaral A, Lukasik K, Szóstek-Mioduchowska A, Pinto-Bravo P, Galvão A, Skarzynski DJ, Ferreira-Dias G. Impairment of the antifibrotic prostaglandin E(2) pathway may influence neutrophil extracellular traps-induced fibrosis in the mare endometrium.. Domest Anim Endocrinol 2019 Apr;67:1-10.
    doi: 10.1016/j.domaniend.2018.10.004pubmed: 30522057google scholar: lookup
  50. Kenney RM, Doig PA. Equine endometrial biopsy. In: Morrow D.A., editor. Current Therapy in Theriogenology 2: Diagnosis, Treatment, and Prevention of Reproductive Diseases in Small and Large Animals. 2nd ed. Saunders WB; Philadelphia, PA, USA: 1986. pp. 723–729.
  51. Schäfer WR, Fischer L, Roth K, Jüllig AK, Stuckenschneider JE, Schwartz P, Weimer M, Orlowska-Volk M, Hanjalic-Beck A, Kranz I, Deppert WR, Zahradnik HP. Critical evaluation of human endometrial explants as an ex vivo model system: a molecular approach.. Mol Hum Reprod 2011 Apr;17(4):255-65.
    doi: 10.1093/molehr/gaq095pubmed: 21115633google scholar: lookup
  52. Zhao S, Fernald RD. Comprehensive algorithm for quantitative real-time polymerase chain reaction.. J Comput Biol 2005 Oct;12(8):1047-64.
    doi: 10.1089/cmb.2005.12.1047pmc: PMC2716216pubmed: 16241897google scholar: lookup
  53. PREDICTED: Equus Caballus Collagen Type I Alpha 2 Chain (COL1A2), mRNA. [(accessed on 9 October 2021)]; Available online: https://www.ncbi.nlm.nih.gov/nucleotide/XM_001492939.4?report=genbank&log$=nuclalign&blast_rank=19&RID=NHF70AB501N.
  54. PREDICTED: Equus Caballus Ribosomal Protein L32 (RPL32), mRNA. [(accessed on 9 October 2021)]; Available online: https://www.ncbi.nlm.nih.gov/nucleotide/XM_001492042.6?report=genbank&log$=nuclalign&blast_rank=1&RID=NHFCEW9V01N.
  55. Posch A, Kohn J, Oh K, Hammond M, Liu N. V3 stain-free workflow for a practical, convenient, and reliable total protein loading control in western blotting.. J Vis Exp 2013 Dec 30;(82):50948.
    doi: 10.3791/50948pmc: PMC4094170pubmed: 24429481google scholar: lookup
  56. Szóstek-Mioduchowska AZ, Lukasik K, Skarzynski DJ, Okuda K. Effect of transforming growth factor -β1 on α-smooth muscle actin and collagen expression in equine endometrial fibroblasts.. Theriogenology 2019 Jan 15;124:9-17.
    doi: 10.1016/j.theriogenology.2018.10.005pubmed: 30321755google scholar: lookup
  57. Szóstek-Mioduchowska AZ, Baclawska A, Okuda K, Skarzynski DJ. Effect of proinflammatory cytokines on endometrial collagen and metallopeptidase expression during the course of equine endometrosis.. Cytokine 2019 Nov;123:154767.
    doi: 10.1016/j.cyto.2019.154767pubmed: 31265984google scholar: lookup
  58. Szóstek-Mioduchowska AZ, Baclawska A, Rebordão MR, Ferreira-Dias G, Skarzynski DJ. Prostaglandins effect on matrix metallopeptidases and collagen in mare endometrial fibroblasts.. Theriogenology 2020 Sep 1;153:74-84.
    doi: 10.1016/j.theriogenology.2020.04.040pubmed: 32442743google scholar: lookup
  59. Ganjam VK, McLeod C, Klesius PH, Washburn SM, Kwapien R, Brown B, Fazeli MH. Effect of ovarian hormones on the phagocytic response of ovariectomized mares.. J Reprod Fertil Suppl 1982;32:169-74.
    pubmed: 6962850
  60. Kumar N, Sood D, van der Spek PJ, Sharma HS, Chandra R. Molecular Binding Mechanism and Pharmacology Comparative Analysis of Noscapine for Repurposing against SARS-CoV-2 Protease.. J Proteome Res 2020 Nov 6;19(11):4678-4689.
    doi: 10.1021/acs.jproteome.0c00367pubmed: 32786685google scholar: lookup
  61. Zughaier S, Karna P, Stephens D, Aneja R. Potent anti-inflammatory activity of novel microtubule-modulating brominated noscapine analogs.. PLoS One 2010 Feb 11;5(2):e9165.
    doi: 10.1371/journal.pone.0009165pmc: PMC2820095pubmed: 20161797google scholar: lookup
  62. Khanmoradi M, Ali Mard S, Aboutaleb N, Nobakht M, Mahmoudian M. The protective activity of noscapine on renal ischemia-reperfusion injury in male Wistar rat.. Iran J Basic Med Sci 2014;17(4):244-9.
    pmc: PMC4046240pubmed: 24904716
  63. Quisbert-Valenzuela EO, Calaf GM. Apoptotic effect of noscapine in breast cancer cell lines.. Int J Oncol 2016 Jun;48(6):2666-74.
    doi: 10.3892/ijo.2016.3476pubmed: 27081867google scholar: lookup
  64. Aneja R, Zhou J, Zhou B, Chandra R, Joshi HC. Treatment of hormone-refractory breast cancer: apoptosis and regression of human tumors implanted in mice.. Mol Cancer Ther 2006 Sep;5(9):2366-77.
    doi: 10.1158/1535-7163.MCT-06-0205pubmed: 16985071google scholar: lookup
  65. Altinoz MA, Topcu G, Hacimuftuoglu A, Ozpinar A, Ozpinar A, Hacker E, Elmaci İ. Noscapine, a Non-addictive Opioid and Microtubule-Inhibitor in Potential Treatment of Glioblastoma.. Neurochem Res 2019 Aug;44(8):1796-1806.
    doi: 10.1007/s11064-019-02837-xpubmed: 31292803google scholar: lookup
  66. Landen JW, Hau V, Wang M, Davis T, Ciliax B, Wainer BH, Van Meir EG, Glass JD, Joshi HC, Archer DR. Noscapine crosses the blood-brain barrier and inhibits glioblastoma growth.. Clin Cancer Res 2004 Aug 1;10(15):5187-201.
    doi: 10.1158/1078-0432.CCR-04-0360pubmed: 15297423google scholar: lookup
  67. Sandbo N, Ngam C, Torr E, Kregel S, Kach J, Dulin N. Control of myofibroblast differentiation by microtubule dynamics through a regulated localization of mDia2.. J Biol Chem 2013 May 31;288(22):15466-73.
    doi: 10.1074/jbc.M113.464461pmc: PMC3668708pubmed: 23580645google scholar: lookup
  68. Sung B, Ahn KS, Aggarwal BB. Noscapine, a benzylisoquinoline alkaloid, sensitizes leukemic cells to chemotherapeutic agents and cytokines by modulating the NF-kappaB signaling pathway.. Cancer Res 2010 Apr 15;70(8):3259-68.
    doi: 10.1158/0008-5472.CAN-09-4230pmc: PMC2855771pubmed: 20354190google scholar: lookup
  69. Chougule MB, Patel AR, Jackson T, Singh M. Antitumor activity of Noscapine in combination with Doxorubicin in triple negative breast cancer.. PLoS One 2011 Mar 15;6(3):e17733.
    doi: 10.1371/journal.pone.0017733pmc: PMC3057970pubmed: 21423660google scholar: lookup
  70. Chandra R. Noscapine as Anticancer Agent & Its Role in Ovarian Cancer. Org. Med. Chem. Int. J. 2019;9:48–56.
    doi: 10.19080/OMCIJ.2019.09.555757google scholar: lookup
  71. Dong J, Ma Q. In Vivo Activation and Pro-Fibrotic Function of NF-κB in Fibroblastic Cells During Pulmonary Inflammation and Fibrosis Induced by Carbon Nanotubes.. Front Pharmacol 2019;10:1140.
    doi: 10.3389/fphar.2019.01140pmc: PMC6783511pubmed: 31632276google scholar: lookup
  72. Shen W, Liang B, Yin J, Li X, Cheng J. Noscapine Increases the Sensitivity of Drug-Resistant Ovarian Cancer Cell Line SKOV3/DDP to Cisplatin by Regulating Cell Cycle and Activating Apoptotic Pathways.. Cell Biochem Biophys 2015 May;72(1):203-13.
    doi: 10.1007/s12013-014-0438-ypubmed: 25510462google scholar: lookup
  73. Domino M, Jasinski T, Kautz E, Juszczuk-Kubiak E, Ferreira-Dias G, Zabielski R, Sady M, Gajewski Z. Expression of genes involved in the NF-κB-dependent pathway of the fibrosis in the mare endometrium.. Theriogenology 2020 Apr 15;147:18-24.
    doi: 10.1016/j.theriogenology.2020.01.055pubmed: 32074495google scholar: lookup
  74. Doddapaneni R, Patel K, Chowdhury N, Singh M. Reversal of drug-resistance by noscapine chemo-sensitization in docetaxel resistant triple negative breast cancer.. Sci Rep 2017 Nov 20;7(1):15824.
    doi: 10.1038/s41598-017-15531-1pmc: PMC5696458pubmed: 29158480google scholar: lookup

Citations

This article has been cited 1 times.
  1. Amaral A, Cebola N, Szóstek-Mioduchowska A, Rebordão MR, Kordowitzki P, Skarzynski D, Ferreira-Dias G. Inhibition of Myeloperoxidase Pro-Fibrotic Effect by Noscapine in Equine Endometrium.. Int J Mol Sci 2023 Feb 10;24(4).
    doi: 10.3390/ijms24043593pubmed: 36835008google scholar: lookup
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