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Home / Equine Research Bank / Animals (Basel) / The Lipidomics of Spermatozoa and Red Blood Cells Membrane P...
Animals : an open access journal from MDPI2022; 13(1); 8; doi: 10.3390/ani13010008

The Lipidomics of Spermatozoa and Red Blood Cells Membrane Profile of Martina Franca Donkey: Preliminary Evaluation.

Abstract: Fatty acid-based lipidomic analysis has been widely used to evaluate health status in human medicine as well as in the veterinary field. In equine species, there has been a developing interest in fertility and sperm quality. Fatty acids, being the principal components of the membranes, play an active role in the regulation of the metabolic activities, and their role on spermiogenesis seems to be of great importance for the resulting quality of the sperm and, thus, fertility. With the application of widely used lipidomic techniques, the aim of this study was to evaluate: (a) the fatty acid content of the spermatozoa's membranes of 26 healthy male Martina Franca donkeys and its possible correlation with sperm parameters, and (b) the evaluation of the composition of the red blood cells' membrane. PUFA omega-6 are the principal components (40.38%) of the total PUFA content (47.79%) in both types of cells; however, DPA is the predominant one on the spermatozoa's membrane (27.57%) but is not present in the erythrocyte's membrane. Spermatozoa's motility (%) is positively correlated with stearic acid and EPA, and progressive motility (%), with oleic acid. These findings offer information on the composition of both types of cells' membranes in healthy male MF donkeys and reflect the metabolic transformations of the spermatozoa's membrane during the maturation period, providing a better perception of the role of fatty acids in sperm parameters and fertility.
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Publication Date: 2022-12-20 PubMed ID: 36611618PubMed Central: PMC9817730DOI: 10.3390/ani13010008Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article is focused on uncovering the lipid profile of the spermatozoa and red blood cells of the Martina Franca donkey species, while also studying the correlation between the fatty acid content in spermatozoa’s membranes and sperm parameters.

Objective of the Research

  • This study primarily aimed to reveal the fatty acid content of the spermatozoa’s membranes in 26 healthy Martina Franca donkeys, and to establish any correlation this may have with sperm parameters. This is because fatty acids play a significant role in regulating metabolic activities and spermiogenesis.
  • Moreover, the research further examined the composition of red blood cells’ membranes in the said species.

Methodology and Results

  • The study employed a series of lipidomic analysis techniques which are commonly used to evaluate health status. This approach not only provides valuable information about the overall health of an organism, but also specifics about the quality of the sperm, and therefore the fertility rate in donkeys.
  • The research found that PUFA omega-6 are the primary components in both cells, accounting for 40.38% of the entire PUFA content. However, DPA (docosapentaenoic acid) was the predominant fatty acid on the spermatozoa’s membrane while it was not present in the erythrocyte’s membrane.
  • An interesting finding was that spermatozoa’s motility was positively correlated with the presence of stearic acid and EPA (eicosapentaenoic acid), while progressive motility was positively associated with oleic acid.

Significance of the Research

  • The findings underscore the direct link between the composition of fatty acids in the spermatozoa’s membrane and the resultant sperm quality. This paves the way towards a better understanding of the role of fatty acids in the establishment of sperm parameters and fertility.
  • The information gathered provides an insightful correlation between specific fatty acids and sperm motility, which could lead to potential advances in male fertility research, particularly in the field of veterinary science dealing with equine species such as donkeys.

Cite This Article

APA
Prasinou P, De Amicis I, Fusaro I, Bucci R, Cavallini D, Parrillo S, Caputo M, Gramenzi A, Carluccio A. (2022). The Lipidomics of Spermatozoa and Red Blood Cells Membrane Profile of Martina Franca Donkey: Preliminary Evaluation. Animals (Basel), 13(1), 8. https://doi.org/10.3390/ani13010008

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 13
Issue: 1
PII: 8

Researcher Affiliations

Prasinou, Paraskevi
  • Faculty of Veterinary Medicine, University of Teramo, Piano d'Accio, 64100 Teramo, Italy.
De Amicis, Ippolito
  • Faculty of Veterinary Medicine, University of Teramo, Piano d'Accio, 64100 Teramo, Italy.
Fusaro, Isa
  • Faculty of Veterinary Medicine, University of Teramo, Piano d'Accio, 64100 Teramo, Italy.
Bucci, Roberta
  • Faculty of Veterinary Medicine, University of Teramo, Piano d'Accio, 64100 Teramo, Italy.
Cavallini, Damiano
  • Department of Veterinary Medical Science, University of Bologna, 40064 Bologna, Italy.
Parrillo, Salvatore
  • Faculty of Veterinary Medicine, University of Teramo, Piano d'Accio, 64100 Teramo, Italy.
Caputo, Maurizio
  • Faculty of Veterinary Medicine, University of Teramo, Piano d'Accio, 64100 Teramo, Italy.
Gramenzi, Alessandro
  • Faculty of Veterinary Medicine, University of Teramo, Piano d'Accio, 64100 Teramo, Italy.
Carluccio, Augusto
  • Faculty of Veterinary Medicine, University of Teramo, Piano d'Accio, 64100 Teramo, Italy.

Grant Funding

  • Project Code: ECS00000041; Project CUP: C43C22000380007 / the European Union - Next Generation EU.

Conflict of Interest Statement

The authors declare no conflict of interest.

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Citations

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