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The Lusitano horse maternal lineage based on mitochondrial D-loop sequence variation.
Abstract: The analysis of mitochondrial D-loop sequences (408 bp) from 145 Lusitano founder mares yielded a total of 27 different haplotypes. The distribution of these mtDNA sequences was quite unequal, with the three most frequent ones representing 56.5% of all the Lusitano founder mares and 14 haplotypes (51.9%) being rare variants found only once in the sampling. Four main haplotype clusters were present in the Lusitano breed. The comparison of these sequences with other equine haplotypes shows that they fall in groups shared with other horse breeds. These data support the hypothesis of multiple domestication events in many distinct geographic areas over a broad time span. However, the analysis of 145 Lusitano, 55 Pura Raza Espanola and 18 Sorraia sequences indicates that half of the samples (50.9%) fall in one specific-cluster (A), which has previously been described as characteristic of the Iberian and Northern African horse breeds. The presence of a phylogeographic structure in cluster A associated with its star-like structure was interpreted as suggestive of a centre of horse domestication in the Iberia Peninsula.
Publication Date: 2005-06-04 PubMed ID: 15932397DOI: 10.1111/j.1365-2052.2005.01279.xGoogle Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research focuses on the genetic history of the Lusitano breed of horses, using mitochondrial D-loop sequences from founder mares and finding a significant amount of haplotype clusters common with other horse breeds. This implies multiple domestication events over time in various geographic locations. However, there is a high prevalence of a specific cluster associated with Iberian and Northern African breeds, suggesting a possible center of horse domestication in the Iberian Peninsula.
Analysis of Mitochondrial D-loop Sequences
- The researchers analyzed mitochondrial D-loop sequences, a part of the DNA present in the mitochondria that serves as an excellent marker for studying evolutionary history and genetic diversity. They examined 408 base pair sequences from 145 Lusitano founder mares.
- The analysis yielded 27 different haplotypes, which are essentially unique combinations of differences (or variations) in the D-loop sequence.
- These genetic variations were not evenly distributed. The three most common sequences represented the majority (56.5%) of the Lusitano founder mares, while half of the total types (14 haplotypes) were rare and only found once in the sample size.
Haplotype Clustering and Comparison with Other Breeds
- These haplotypes formed four main clusters in the Lusitano breed.
- When compared to haplotypes of other horse breeds, the researchers found that these clusters fall in groups shared with those breeds. This finding supports the theory of multiple domestication events taking place across different areas and periods.
Indications of Iberian Peninsula Domestication
- The researchers expanded their analysis to include 55 Pura Raza Espanola and 18 Sorraia sequences. They found that half of the total samples (50.9%) fall into a specific cluster (A).
- This cluster A has been described earlier as common in the Iberian and Northern African horse breeds.
- The high frequency of this cluster, along with its phylogeographic structure (the geographical distribution of genetic traits), indicates the possibility of a center of horse domestication in the Iberian Peninsula.
Cite This Article
APA
Lopes MS, Mendonça D, Cymbron T, Valera M, da Costa-Ferreira J, Machado Ada C.
(2005).
The Lusitano horse maternal lineage based on mitochondrial D-loop sequence variation.
Anim Genet, 36(3), 196-202.
https://doi.org/10.1111/j.1365-2052.2005.01279.x Publication
Researcher Affiliations
- Department of Agriculture, Biotechnology Centre of Azores, University of Azores, Terra-Chã, 9701-851 Angra do Heroísmo, Portugal.
MeSH Terms
- Animals
- Base Sequence
- Cluster Analysis
- Computational Biology
- DNA, Mitochondrial / genetics
- Databases, Nucleic Acid
- Female
- Genetic Variation
- Geography
- Haplotypes / genetics
- Horses / genetics
- Molecular Sequence Data
- Phylogeny
- Sequence Alignment
- Sequence Analysis, DNA
- Species Specificity
Citations
This article has been cited 12 times.- Sheikh A. Mitochondrial DNA sequencing of Kehilan and Hamdani horses from Saudi Arabia. Saudi J Biol Sci 2023 Sep;30(9):103741.
- Liu S, Fu C, Yang Y, Zhang Y, Ma H, Xiong Z, Ling Y, Zhao C. Current genetic conservation of Chinese indigenous horses revealed with Y-chromosomal and mitochondrial DNA polymorphisms. G3 (Bethesda) 2021 Feb 9;11(2).
- Effa K, Rosenbom S, Han J, Dessie T, Beja-Pereira A. Genetic Diversities and Historical Dynamics of Native Ethiopian Horse Populations (Equus caballus) Inferred from Mitochondrial DNA Polymorphisms. Genes (Basel) 2021 Jan 25;12(2).
- Dell AC, Curry MC, Yarnell KM, Starbuck GR, Wilson PB. Mitochondrial D-loop sequence variation and maternal lineage in the endangered Cleveland Bay horse. PLoS One 2020;15(12):e0243247.
- Ianella P, Albuquerque MSM, Paiva SR, Egito AA, Almeida LD, Sereno FTPS, Carvalho LFR, Mariante AS, McManus CM. D-loop haplotype diversity in Brazilian horse breeds. Genet Mol Biol 2017 Jul-Sep;40(3):604-609.
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- Takasu M, Ishihara N, Tozaki T, Kakoi H, Maeda M, Mukoyama H. Genetic diversity of maternal lineage in the endangered Kiso horse based on polymorphism of the mitochondrial DNA D-loop region. J Vet Med Sci 2014 Nov;76(11):1451-6.
- Devi KM, Ghosh SK. Molecular phylogeny of Indian horse breeds with special reference to Manipuri pony based on mitochondrial D-loop. Mol Biol Rep 2013 Oct;40(10):5861-7.
- Achilli A, Olivieri A, Soares P, Lancioni H, Hooshiar Kashani B, Perego UA, Nergadze SG, Carossa V, Santagostino M, Capomaccio S, Felicetti M, Al-Achkar W, Penedo MC, Verini-Supplizi A, Houshmand M, Woodward SR, Semino O, Silvestrelli M, Giulotto E, Pereira L, Bandelt HJ, Torroni A. Mitochondrial genomes from modern horses reveal the major haplogroups that underwent domestication. Proc Natl Acad Sci U S A 2012 Feb 14;109(7):2449-54.
- Priskin K, Szabó K, Tömöry G, Bogácsi-Szabó E, Csányi B, Eördögh R, Downes CS, Raskó I. Mitochondrial sequence variation in ancient horses from the Carpathian Basin and possible modern relatives. Genetica 2010 Feb;138(2):211-8.
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