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Clinics in laboratory medicine2011; 31(1); 109-123; doi: 10.1016/j.cll.2010.12.001

The regenerative medicine laboratory: facilitating stem cell therapy for equine disease.

Abstract: This article focuses on the emerging field of equine regenerative medicine with an emphasis on the use of mesenchymal stem cells (MSCs) for orthopedic diseases. We detail laboratory procedures and protocols for tissue handling and MSC isolation, characterization, expansion, and cryopreservation from bone marrow, fat, and placental tissues. We provide an overview of current clinical uses for equine MSCs and how MSCs function to heal tissues. Current laboratory practices in equine regenerative medicine mirror those in the human field. However, the translational use of autologous and allogeneic MSCs for patient therapy far exceeds what is currently permitted in human medicine.
Copyright © 2011 Elsevier Inc. All rights reserved.
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Publication Date: 2011-02-08 PubMed ID: 21295725DOI: 10.1016/j.cll.2010.12.001Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article centers on the developing area of regenerative medicine in horses, specifically using mesenchymal stem cells (MSCs) for treating orthopedic diseases. It provides a comprehensive view of various laboratory procedures for handling tissues and MSC isolation, its characterization and expansion, and methods of preserving these cells obtained from bone marrow, fat, and placental tissues.

Equine Regenerative Medicine

  • The study predominantly revolves around the promising field of equine regenerative medicine. This branch of medicine concentrates on replacing, engineering or regenerating cells, tissues or organs to restore or establish normal function.
  • In particular, the article underlines the therapeutic potential of mesenchymal stem cells (MSCs) in treating orthopedic diseases in horses. These MSCs, being multipotent stromal cells, have the capacity to differentiate into a variety of cell types and thus can contribute to the healing of tissues.

Laboratory Procedures and Protocols

  • The research elaborates on several laboratory protocols and procedures, which involve handling of tissues, isolation of MSCs. The study offers insights into characterizing, studying the growth patterns of, and cryopreservation – or freezing of cells for later use – of MSCs.
  • The sources for these MSCs can be varied, such as bone marrow, fat tissues, or placental tissues. The study also indicates that these methods of extracting and preserving MSCs are akin to those deployed in human regenerative medicine.

Clinical Application and Future Scope

  • The article also provides a snapshot of current clinical applications of MSCs in equine medicine. More importantly, it illustrates how these cells function to repair and heal tissues, which is a central aspect of any regenerative therapy.
  • One of the noteworthy aspects of this study is the observation that the clinical use of autologous (derived from the same individual’s body) and allogeneic (originating from a different individual of the same species) MSCs in equine medicine outnumber what’s currently allowed in human medicine. This potentially signals the scope of advancements in the field of equine regenerative medicine over its human counterpart.

Cite This Article

APA
Borjesson DL, Peroni JF. (2011). The regenerative medicine laboratory: facilitating stem cell therapy for equine disease. Clin Lab Med, 31(1), 109-123. https://doi.org/10.1016/j.cll.2010.12.001

Publication

Clinics in laboratory medicine
ISSN: 1557-9832
NlmUniqueID: 8100174
Country: United States
Language: English
Volume: 31
Issue: 1
Pages: 109-123

Researcher Affiliations

Borjesson, Dori L
  • Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, 95616, USA. dlborjesson@ucdavis.edu
Peroni, John F

    MeSH Terms

    • Animals
    • Cartilage / cytology
    • Cartilage / transplantation
    • Cell Culture Techniques
    • Cell Differentiation
    • Horse Diseases / therapy
    • Horses
    • Legislation, Veterinary
    • Mesenchymal Stem Cell Transplantation / ethics
    • Mesenchymal Stem Cell Transplantation / legislation & jurisprudence
    • Mesenchymal Stem Cell Transplantation / veterinary
    • Mesenchymal Stem Cells / cytology
    • Mesenchymal Stem Cells / immunology
    • Mesenchymal Stem Cells / physiology
    • Regenerative Medicine / methods
    • Regenerative Medicine / trends
    • Tissue Transplantation / ethics
    • Tissue Transplantation / legislation & jurisprudence
    • Tissue Transplantation / veterinary
    • Tissue and Organ Harvesting / methods
    • Veterinary Medicine / methods
    • Veterinary Medicine / trends

    Citations

    This article has been cited 20 times.
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