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The veterinary quarterly2015; 35(2); 88-96; doi: 10.1080/01652176.2015.1021186

The role of microRNAs in equine medicine: a review.

Abstract: The search for new markers of diseases in human as well as veterinary medicine is ongoing. Recently, microRNAs (miRNAs or miRs) have emerged as potential new biomarkers. MiRNAs are short sequences of RNA (∼22 nucleotides) that regulate gene expression via their target messenger RNA (mRNA). Circulating miRNAs in blood can be used as novel diagnostic markers for diseases due to their evolutionary conservation and stability. As a consequence of their systemic and manifold effects on the gene expression in various target organs, the concept that miRNAs could function as hormones has been suggested. This review summarizes the biogenesis, maturation, and stability of miRNAs and discusses their use as potential biomarkers in equine medicine. To date, over 700 equine miRNAs are identified with distinct subsets of miRNAs differentially expressed in a tissue-specific manner. A physiological involvement of various miRNAs in the regulation of cell survival, steroidogenesis, and differentiation during follicle selection and ovulation in the monovular equine ovary has been demonstrated. Furthermore, miRNAs might be used as novel diagnostic markers for myopathies such as polysaccharide storage myopathy and recurrent exertional rhabdomyolysis as well as osteochondrosis. Preliminary data indicate that miRNAs in blood might play important roles in equine glucose metabolism pathway. Of note, breed differences have been reported regarding the normal equine miRNA signature. For disease prevention, it is of utmost importance to identify disease-associated biomarkers which help detect diseases before symptoms appear. As such, circulating miRNAs represent promising novel diagnostic markers in equine medicine.
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Publication Date: 2015-03-24 PubMed ID: 25695624DOI: 10.1080/01652176.2015.1021186Google Scholar: Lookup
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  • Non-U.S. Gov't
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article presents an exploration into the use of microRNAs (miRNAs), short RNA sequences that regulate gene expression, as biomarkers for disease detection in horses. These miRNAs may also function like hormones due to their systemic effects on gene expression in different body organs. Over 700 miRNAs have been identified in horses, with some showing great promise as diagnostic markers for myopathies and metabolic conditions.

Biogenesis, Maturation, and Stability of miRNAs

  • The review covers the life cycle of miRNAs, from their creation to their final mature form.
  • miRNAs are noted for their stability, which makes them reliable markers in blood tests.
  • These RNA sequences regulate gene expression by targeting messenger RNAs (mRNAs), making them key players in cellular function and response to disease.

miRNAs as Biomarkers in Equine Medicine

  • The variety and specificity of miRNAs makes them ripe for exploration as novel biomarkers in veterinary medicine, particularly for equine diseases.
  • The study identifies over 700 unique miRNAs in horses, some of which show distinct expression patterns depending on the tissue type.
  • In addition, the research finds connections between miRNAs and various equine physiological processes like cell survival, steroidogenesis, follicle selection, and ovulation.

miRNAs in Equine Disease Diagnosis and Metabolism

  • Specific miRNAs are proposed as diagnostic tools for equine myopathies, including polysaccharide storage myopathy and recurrent exertional rhabdomyolysis, and developmental conditions like osteochondrosis.
  • The article also discusses preliminary findings that suggest miRNAs may play a substantial role in equine glucose metabolism.

Breed Differences in miRNA Signatures

  • There are noted differences in the normal miRNA profiles between different horse breeds.
  • Recognizing these differences is key to developing accurate, breed-specific diagnostic tools.

Implications for Disease Prevention

  • By identifying disease-associated biomarkers, veterinarians can potentially detect diseases before symptoms appear, leading to more effective treatments and better health outcomes for horses.
  • Circulating miRNAs prove to be promising in this regard, opening new avenues for preventive equine medicine.

Cite This Article

APA
van der Kolk JH, Pacholewska A, Gerber V. (2015). The role of microRNAs in equine medicine: a review. Vet Q, 35(2), 88-96. https://doi.org/10.1080/01652176.2015.1021186

Publication

The veterinary quarterly
ISSN: 1875-5941
NlmUniqueID: 7909485
Country: England
Language: English
Volume: 35
Issue: 2
Pages: 88-96

Researcher Affiliations

van der Kolk, J H
  • a Department of Clinical Veterinary Medicine, Vetsuisse Faculty, Swiss Institute for Equine Medicine (ISME) , University of Bern and Agroscope , Länggassstrasse 124, 3012 Bern , Switzerland.
Pacholewska, A
    Gerber, V

      MeSH Terms

      • Animals
      • Biomarkers / blood
      • Fertility / physiology
      • Gene Expression
      • Horse Diseases / blood
      • Horses
      • Humans
      • MicroRNAs / blood
      • MicroRNAs / genetics
      • MicroRNAs / metabolism
      • Polymorphism, Genetic
      • Reverse Transcriptase Polymerase Chain Reaction

      Citations

      This article has been cited 12 times.
      1. Sisia G, Giudice E, Attanzio A, Briglia M, Piccione G, Trunfio C, Arfuso F. Exosome and miRNA Content Engagement in the Physical Exercise Response: What Is Known to Date in Atheltic Horses?. Int J Mol Sci 2026 Jan 4;27(1).
        doi: 10.3390/ijms27010520pubmed: 41516392google scholar: lookup
      2. Yuan X, Yao X, Zeng Y, Wang J, Ren W, Wang T, Li X, Yang L, Yang X, Meng J. The Impact of the Competition on miRNA, Proteins, and Metabolites in the Blood Exosomes of the Yili Horse. Genes (Basel) 2025 Feb 15;16(2).
        doi: 10.3390/genes16020224pubmed: 40004554google scholar: lookup
      3. Pfeifer JPH, Stievani FC, Fernandes CJDC, Rosa GDS, Apolonio EVP, Rossi MC, Zambuzzi WF, Alves ALG. Influence of inflammation on the expression of microRNA-140 in extracellular vesicles from 2D and 3D culture models of synovial-membrane-derived stem cells. Front Bioeng Biotechnol 2024;12:1416694.
        doi: 10.3389/fbioe.2024.1416694pubmed: 39170063google scholar: lookup
      4. Libera K, Konieczny K, Grabska J, Smulski S, Szczerbal I, Szumacher-Strabel M, Pomorska-Mól M. Potential Novel Biomarkers for Mastitis Diagnosis in Sheep. Animals (Basel) 2021 Sep 24;11(10).
        doi: 10.3390/ani11102783pubmed: 34679803google scholar: lookup
      5. Lee S, Baker ME, Clinton M, Taylor SE. Use of Omics Data in Fracture Prediction; a Scoping and Systematic Review in Horses and Humans. Animals (Basel) 2021 Mar 30;11(4).
        doi: 10.3390/ani11040959pubmed: 33808497google scholar: lookup
      6. Dhama K, Latheef SK, Dadar M, Samad HA, Munjal A, Khandia R, Karthik K, Tiwari R, Yatoo MI, Bhatt P, Chakraborty S, Singh KP, Iqbal HMN, Chaicumpa W, Joshi SK. Biomarkers in Stress Related Diseases/Disorders: Diagnostic, Prognostic, and Therapeutic Values. Front Mol Biosci 2019;6:91.
        doi: 10.3389/fmolb.2019.00091pubmed: 31750312google scholar: lookup
      7. Smieszek A, Kornicka K, Szłapka-Kosarzewska J, Androvic P, Valihrach L, Langerova L, Rohlova E, Kubista M, Marycz K. Metformin Increases Proliferative Activity and Viability of Multipotent Stromal Stem Cells Isolated from Adipose Tissue Derived from Horses with Equine Metabolic Syndrome. Cells 2019 Jan 22;8(2).
        doi: 10.3390/cells8020080pubmed: 30678275google scholar: lookup
      8. Cappelli K, Capomaccio S, Viglino A, Silvestrelli M, Beccati F, Moscati L, Chiaradia E. Circulating miRNAs as Putative Biomarkers of Exercise Adaptation in Endurance Horses. Front Physiol 2018;9:429.
        doi: 10.3389/fphys.2018.00429pubmed: 29740341google scholar: lookup
      9. Pacholewska A, Kraft MF, Gerber V, Jagannathan V. Differential Expression of Serum MicroRNAs Supports CD4⁺ T Cell Differentiation into Th2/Th17 Cells in Severe Equine Asthma. Genes (Basel) 2017 Dec 12;8(12).
        doi: 10.3390/genes8120383pubmed: 29231896google scholar: lookup
      10. McGivney BA, Griffin ME, Gough KF, McGivney CL, Browne JA, Hill EW, Katz LM. Evaluation of microRNA expression in plasma and skeletal muscle of thoroughbred racehorses in training. BMC Vet Res 2017 Nov 22;13(1):347.
        doi: 10.1186/s12917-017-1277-zpubmed: 29166903google scholar: lookup
      11. Pacholewska A, Mach N, Mata X, Vaiman A, Schibler L, Barrey E, Gerber V. Novel equine tissue miRNAs and breed-related miRNA expressed in serum. BMC Genomics 2016 Oct 26;17(1):831.
        doi: 10.1186/s12864-016-3168-2pubmed: 27782799google scholar: lookup
      12. Unger L, Fouché N, Leeb T, Gerber V, Pacholewska A. Optimized methods for extracting circulating small RNAs from long-term stored equine samples. Acta Vet Scand 2016 Jun 29;58(1):44.
        doi: 10.1186/s13028-016-0224-5pubmed: 27356979google scholar: lookup
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