The similarity of mitochondrial distribution in equine skeletal muscles of differing oxidative capacity.
Abstract: A morphometric analysis was performed on horse muscle tissue to quantify mitochondrial distribution relative to capillaries. Samples of M. vastus medialis, M. semitendinosus, M. masseter and M. cutaneus thoracicus were preserved in a glutaraldehyde fixative for electron microscopy, or frozen for biochemical and histochemical analysis. These four muscles varied from highly oxidative in type, consisting nearly completely of type I fibres, in masseter, to highly glycolytic, primarily type IIb fibres, in cutaneus. In all four muscles, mitochondria were found in highest volume density near capillaries at the fibre border, with a sharp decline in volume density towards the fibre centre. This distribution was independent of myoglobin concentration, muscle fibre type and the activities of three key metabolic enzymes, citrate synthase, 3-OH-acyl-CoA dehydrogenase and lactate dehydrogenase.
Publication Date: 1988-07-01 PubMed ID: 3209968DOI: 10.1242/jeb.137.1.253Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Research Support
- U.S. Gov't
- Non-P.H.S.
Summary
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This research article discusses a study in which the distribution of mitochondria in different types of horse muscle tissue was analyzed and found to be largely similar, regardless of factors such as fiber type and key metabolic enzyme activity.
Objective of the Study
- The main goal of this study was to analyze and quantify the distribution of mitochondria in relation to capillaries in various types of horse muscle tissue.
- The researchers wanted to see if there were notable distribution differences based on muscle fibers’ oxidative capacity or the activities of key metabolic enzymes.
Methods and Materials
- The muscle tissue samples for this study were taken from four different muscles of horses: M. vastus medialis, M. semitendinosus, M. masseter and M. cutaneus thoracicus.
- The tissues were either preserved using a glutaraldehyde fixative for examination under electron microscopy or frozen for biochemical and histochemical analyses.
- The chosen muscles varied in their oxidative profile, ranging from highly oxidative, primarily composed of type I fibres in M. masseter, to highly glycolytic, characterized mainly by type IIb fibres in M. cutaneus thoracicus.
Key Findings
- The study discovered that mitochondria were predominantly found near capillaries at the fiber border, with a significant drop in volume density towards the fibre center.
- This pattern of mitochondrial distribution was remarkably consistent across all four muscles studied, regardless of their oxidative nature or fiber type.
- The mitochondrial distribution was also found to be independent of myoglobin concentration and the activities of three key metabolic enzymes: citrate synthase, 3-OH-acyl-CoA dehydrogenase, and lactate dehydrogenase.
Conclusion
- The findings of the study suggest a universal pattern of mitochondrial distribution in horse muscles, irrespective of their oxidative capacity or other biochemical characteristics.
Cite This Article
APA
Kayar SR, Hoppeler H, Essen-Gustavsson B, Schwerzmann K.
(1988).
The similarity of mitochondrial distribution in equine skeletal muscles of differing oxidative capacity.
J Exp Biol, 137, 253-263.
https://doi.org/10.1242/jeb.137.1.253 Publication
Researcher Affiliations
- Institute of Anatomy, University of Berne, Switzerland.
MeSH Terms
- 3-Hydroxyacyl CoA Dehydrogenases / metabolism
- Animals
- Citrate (si)-Synthase / metabolism
- Horses / metabolism
- L-Lactate Dehydrogenase / metabolism
- Microscopy, Electron
- Mitochondria, Muscle / metabolism
- Mitochondria, Muscle / ultrastructure
- Muscles / anatomy & histology
- Organ Specificity
- Oxygen Consumption
Citations
This article has been cited 10 times.- Levitt DG, Levitt JE, Levitt MD. Quantitative Assessment of Blood Lactate in Shock: Measure of Hypoxia or Beneficial Energy Source. Biomed Res Int 2020;2020:2608318.
- Valdivieso P, Toigo M, Hoppeler H, Flück M. T/T homozygosity of the tenascin-C gene polymorphism rs2104772 negatively influences exercise-induced angiogenesis. PLoS One 2017;12(4):e0174864.
- Poulsen CB, Damkjær M, Hald BO, Wang T, Holstein-Rathlou NH, Jacobsen JC. Vascular flow reserve as a link between long-term blood pressure level and physical performance capacity in mammals. Physiol Rep 2016 Jun;4(11).
- Desplanches D, Amami M, Dupré-Aucouturier S, Valdivieso P, Schmutz S, Mueller M, Hoppeler H, Kreis R, Flück M. Hypoxia refines plasticity of mitochondrial respiration to repeated muscle work. Eur J Appl Physiol 2014 Feb;114(2):405-17.
- Picard M, White K, Turnbull DM. Mitochondrial morphology, topology, and membrane interactions in skeletal muscle: a quantitative three-dimensional electron microscopy study. J Appl Physiol (1985) 2013 Jan 15;114(2):161-71.
- Hardy KM, Dillaman RM, Locke BR, Kinsey ST. A skeletal muscle model of extreme hypertrophic growth reveals the influence of diffusion on cellular design. Am J Physiol Regul Integr Comp Physiol 2009 Jun;296(6):R1855-67.
- Shaw CS, Sherlock M, Stewart PM, Wagenmakers AJ. Adipophilin distribution and colocalization with lipid droplets in skeletal muscle. Histochem Cell Biol 2009 May;131(5):575-81.
- Shaw CS, Jones DA, Wagenmakers AJ. Network distribution of mitochondria and lipid droplets in human muscle fibres. Histochem Cell Biol 2008 Jan;129(1):65-72.
- Reichmann H, Wildenauer D. Quantitative succinate dehydrogenase analysis in normal and ragged-red muscle fibers. Histochemistry 1991;96(3):251-3.
- Blanco CE, Fournier M, Sieck GC. Metabolic variability within individual fibres of the cat tibialis posterior and diaphragm muscles. Histochem J 1991 Aug;23(8):366-74.
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