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Home / Equine Research Bank / Osteoarthritis Cartilage / The use of a cartilage decellularized matrix scaffold for th...
Osteoarthritis and cartilage2016; 25(3); 413-420; doi: 10.1016/j.joca.2016.08.005

The use of a cartilage decellularized matrix scaffold for the repair of osteochondral defects: the importance of long-term studies in a large animal model.

Abstract: To investigate the effect of decellularized cartilage-derived matrix (CDM) scaffolds, by itself and as a composite scaffold with a calcium phosphate (CaP) base, for the repair of osteochondral defects. It was hypothesized that the chondral defects would heal with fibrocartilaginous tissue and that the composite scaffold would result in better bone formation. After an 8-week pilot experiment in a single horse, scaffolds were implanted in eight healthy horses in osteochondral defects on the medial trochlear ridge of the femur. In one joint a composite CDM-CaP scaffold was implanted (+P), in the contralateral joint a CDM only (-P) scaffold. After euthanasia at 6 months, tissues were analysed by histology, immunohistochemistry, micro-CT, biochemistry and biomechanical evaluation. The 8-week pilot showed encouraging formation of bone and cartilage, but incomplete defect filling. At 6 months, micro-CT and histology showed much more limited filling of the defect, but the CaP component of the +P scaffolds was well integrated with the surrounding bone. The repair tissue was fibrotic with high collagen type I and low type II content and with no differences between the groups. There were also no biochemical differences between the groups and repair tissue was much less stiff than normal tissue (P < 0.0001). The implants failed to produce reasonable repair tissue in this osteochondral defect model, although the CaP base in the -P group integrated well with the recipient bone. The study stresses the importance of long-term in vivo studies to assess the efficacy of cartilage repair techniques.
Copyright © 2016 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.
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Publication Date: 2016-08-20 PubMed ID: 27554995PubMed Central: PMC7116104DOI: 10.1016/j.joca.2016.08.005Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article investigates the effectiveness of a scaffold made from decellularized cartilage-derived matrix (CDM) in healing cartilage defects. The study was conducted on horses over a six-month period and concluded that despite promising results from a short-term experiment, longer-term studies did not demonstrate beneficial results.

Objective of the Research

  • The primary goal of the research was to examine the performance of decellularized cartilage-derived matrix (CDM) scaffolds in repairing osteochondral defects, essentially damage to cartilage and underlying bone. A secondary objective was to compare the effectiveness of a scaffold made solely from CDM with a composite scaffold made from CDM and calcium phosphate, a substance commonly used in bone repair.

Research Method

  • Initially, an 8-week pilot experiment was conducted in a sole horse, followed by the main study of eight healthy horses. In each animal, the scaffolds were implanted in cartilage defects in the knee joint.
  • In one joint of each horse, a scaffold made of CDM and calcium phosphate was applied, and in the contrasting joint, a scaffold made solely of CDM was used.
  • Six months post-implant, the horses were euthanized, and the tissue around the scaffolds was analyzed through various techniques including histology, immunohistochemistry, micro-CT, biochemistry, and biomechanical evaluation.

Study Findings

  • While preliminary results showed promising bone and cartilage formation, over a period of six months filling of the defect was limited.
  • The composite scaffold that included calcium phosphate integrated well with the adjacent bone, although overall repair was inadequate.
  • There was no significant difference between the two scaffold groups, and the repair tissue was less stiff than normal tissue and had poor cartilage growth, indicating ineffective repair.
  • The study concluded by underlining the significance of long-term in vivo studies to assess the efficiency of cartilage repair techniques. Short-term results were misleading, with initial bone and cartilage growth not resulting in effective healing over time.

Cite This Article

APA
Vindas Bolaños RA, Cokelaere SM, Estrada McDermott JM, Benders KE, Gbureck U, Plomp SG, Weinans H, Groll J, van Weeren PR, Malda J. (2016). The use of a cartilage decellularized matrix scaffold for the repair of osteochondral defects: the importance of long-term studies in a large animal model. Osteoarthritis Cartilage, 25(3), 413-420. https://doi.org/10.1016/j.joca.2016.08.005

Publication

Osteoarthritis and cartilage
ISSN: 1522-9653
NlmUniqueID: 9305697
Country: England
Language: English
Volume: 25
Issue: 3
Pages: 413-420
PII: S1063-4584(16)30239-4

Researcher Affiliations

Vindas Bolaños, R A
  • Cátedra de Cirugía de Especies Mayores, Escuela de Medicina Veterinaria, Universidad Nacional, Heredia, Costa Rica.
Cokelaere, S M
  • Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, The Netherlands.
Estrada McDermott, J M
  • Cátedra de Cirugía de Especies Mayores, Escuela de Medicina Veterinaria, Universidad Nacional, Heredia, Costa Rica.
Benders, K E M
  • Department of Orthopaedics, Division of Surgery, University Medical Center Utrecht, Utrecht, The Netherlands.
Gbureck, U
  • Department of Functional Materials in Medicine and Dentistry, University of Würzburg, Würzburg, Germany.
Plomp, S G M
  • Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, The Netherlands.
Weinans, H
  • Department of Orthopaedics, Division of Surgery, University Medical Center Utrecht, Utrecht, The Netherlands.
Groll, J
  • Department of Functional Materials in Medicine and Dentistry, University of Würzburg, Würzburg, Germany.
van Weeren, P R
  • Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, The Netherlands.
Malda, J
  • Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, The Netherlands; Department of Orthopaedics, Division of Surgery, University Medical Center Utrecht, Utrecht, The Netherlands. Electronic address: j.malda@umcutrecht.nl.

MeSH Terms

  • Animals
  • Cartilage / metabolism
  • Cartilage, Articular / diagnostic imaging
  • Cartilage, Articular / injuries
  • Cartilage, Articular / pathology
  • Disease Models, Animal
  • Horses
  • Tissue Scaffolds
  • X-Ray Microtomography

Grant Funding

  • 647426 / European Research Council

Conflict of Interest Statement

. The authors have nothing to disclose.

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