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Veterinary clinical pathology2024; 53(3); 358-368; doi: 10.1111/vcp.13372

The use of serum paraoxonase-1 to assess inflammation in horses with colitis.

Abstract: Paraoxonase-1 (PON-1) has been suggested as a marker of inflammation and oxidative stress in horses and could potentially be used for prognostication in horses with colitis. Objective: Assessment of PON-1 in horses with colitis and comparison of two methods. Methods: Serum PON-1 was measured by two methods (paraoxon and p-nitrophenyl acetate) in 161 horses with colitis and 57 controls. Follow-up samples obtained during hospitalization were available from 106 horses with colitis. The two methods were compared. Results: Serum PON-1 was significantly lower in horses with colitis than in healthy horses (P < .0001 for both methods) as well as in nonsurvivors compared with survivors (P = .0141 [paraoxon-based method] and P < .0001 [p-nitrophenyl acetate-based method]), but with marked overlap between groups. PON-1 activity did not change parallel to a change in inflammatory status in response to treatment when assessed at admission and in up to seven follow-up samples. Admission PON-1 activity could not reliably classify horses as survivors or nonsurvivors, with sensitivity and specificity ranging between 53.1% and 72.9%. Results from the two methods were comparable. Conclusions: Both methods reliably measured serum PON-1 activity. Significant differences in PON-1 activity were found between healthy horses and horses with colitis and between survivors and nonsurvivors. However, PON-1 activity varied considerably within groups. Both the proposed reference intervals as well as alternative cutoff values resulted in suboptimal diagnostic and prognostic performance, and the use of serum PON-1 in horses with colitis thus seems to add little to existing diagnostic and prognostic markers.
© 2024 The Author(s). Veterinary Clinical Pathology published by Wiley Periodicals LLC on behalf of American Society for Veterinary Clinical Pathology.
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Publication Date: 2024-07-21 PubMed ID: 39034301DOI: 10.1111/vcp.13372Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigated the use of serum paraoxonase-1 (PON-1) as a marker for inflammation in horses with colitis, and also compared the efficacy of two methods of measuring PON-1. The study deduced that both methods of measurement recorded significant lower PON-1 activity in horses affected by colitis and non-survivals when compared with their healthier and survival counterparts, respectively. However, PON-1 measurements proved inconsistent and therefore, does not present a reliable diagnostic or prognostic marker.

Objectives and Methodology

  • This study had two main objectives. Firstly, to assess the role of Paraoxonase-1 (PON-1) in horses with colitis. PON-1 is an enzyme that’s been marked as an indicator of inflammation and oxidative stress in horses, significance in the prognosis of horses with colitis. Secondly, the research aimed to compare two measurement methods of PON-1 activity – paraoxon and p-nitrophenyl acetate.
  • The researchers measured serum PON-1 in 161 horses suffering from colitis and 57 healthy control horses using the aforementioned methods.
  • Follow-up samples were obtained from 106 horses over their hospitalization period to monitor changes in PON-1 activity and to verify whether it mirrored any change in the inflammatory status of the horses in response to treatment.

Results

  • The findings revealed that the serum PON-1 activity was notably lower in horses suffering from colitis than in healthy horses. This was consistent across both measurement methods with statistically significant results.
  • The study also found that the serum PON-1 in non-surviving horses was lower than in those that survived. This applied to both measurement methods and was statistically significant.
  • Despite these findings, there was a marked overlap in PON-1 activity levels within the groups of horses.
  • The researchers observed that the PON-1 activity level did not show consistent changes in line with the inflammatory status of the horses over the course of their treatment.
  • PON-1 activity levels at admission could not dependably predict survival or non-survival, as the sensitivity and specificity of this method ranged between 53.1% and 72.9%.

Conclusions

  • The results confirmed that both assessment methods could reliably measure serum PON-1 activity.
  • Even though notable differences in PON-1 activity were found between healthy horses and those with colitis, and between survivors and non-survivors, the levels fluctuated immensely within each group of horses.
  • Due to this inconsistency, the use of PON-1 as a diagnostic and prognostic tool in horses with colitis appeared to be limited, as both the proposed reference intervals and alternative cutoff values showed less than optimum diagnostic and prognostic performance.

Cite This Article

APA
Winther MF, Johnsson J, Madsen PK, Pihl TH, Paltrinieri S, Cerón JJ, Scavone D, Pardo-Marin L, Jacobsen S. (2024). The use of serum paraoxonase-1 to assess inflammation in horses with colitis. Vet Clin Pathol, 53(3), 358-368. https://doi.org/10.1111/vcp.13372

Publication

Veterinary clinical pathology
ISSN: 1939-165X
NlmUniqueID: 9880575
Country: United States
Language: English
Volume: 53
Issue: 3
Pages: 358-368

Researcher Affiliations

Winther, Malou F
  • Department of Veterinary Clinical Sciences, University of Copenhagen, Copenhagen, Denmark.
Johnsson, Josefine
  • Department of Veterinary Clinical Sciences, University of Copenhagen, Copenhagen, Denmark.
Madsen, Pernille K
  • Department of Veterinary Clinical Sciences, University of Copenhagen, Copenhagen, Denmark.
Pihl, Tina H
  • Department of Veterinary Clinical Sciences, University of Copenhagen, Copenhagen, Denmark.
Paltrinieri, Saverio
  • Department of Veterinary Medicine, University of Milan, Milan, Italy.
Cerón, José J
  • Department of Animal Medicine and Surgery, University of Murcia, Murcia, Spain.
Scavone, Donatella
  • Department of Veterinary Medicine, University of Milan, Milan, Italy.
Pardo-Marin, Luis
  • Department of Animal Medicine and Surgery, University of Murcia, Murcia, Spain.
Jacobsen, Stine
  • Department of Veterinary Clinical Sciences, University of Copenhagen, Copenhagen, Denmark.

MeSH Terms

  • Animals
  • Horses
  • Aryldialkylphosphatase / blood
  • Horse Diseases / blood
  • Horse Diseases / diagnosis
  • Colitis / veterinary
  • Colitis / blood
  • Colitis / diagnosis
  • Inflammation / veterinary
  • Inflammation / blood
  • Male
  • Female
  • Biomarkers / blood

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