Three-dimensional reconstruction of the equine ovary.
Abstract: The equine ovary has a very unique structure in terms of its extreme large size, the presence of the ovulation fossa and the inverted location of its cortex and medulla. In the previous study, it was recognized that the application of three-dimensional internal structure microscopy (3D-ISM) to observe the mare ovary is very effective. Three-dimensional reconstruction of serially sliced images made by 3D-ISM was successful in this study with the aid of the sophisticated image processing technique. The rotation of the reconstructed ovary has been carried out with and without the application of the transparency technique in the ovarian stromal region. The spatial localization of follicles and corpus luteum was clearly visualized by rotating the reconstructed image of the ovary. The extraction of the images of follicles and corpus luteum was also available and gave a quantifiable understanding of their structure.
Publication Date: 2005-01-15 PubMed ID: 15649227DOI: 10.1111/j.1439-0264.2004.00567.xGoogle Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research article describes a successful study that applied three-dimensional internal structure microscopy (3D-ISM) to understand the unique structure of the equine ovary better, providing a clearer visualisation of the spatial localisation of follicles and corpus luteum.
Research Methodology
- The researchers conducted a study to investigate the unique structure of the equine ovary, which is extremely large and characterised by the presence of an ovulation fossa and the inverted location of its cortex and medulla.
- Scientists applied a previously effective technology called three-dimensional internal structure microscopy (3D-ISM) to serially sliced images of the ovaries.
- The sliced images were then processed and reconstructed to form a three-dimensional image using a sophisticated image processing technique.
Results of the Research
- They successfully reconstructed the sliced images into a three-dimensional ovary model.
- This allowed them to execute a rotation of the constructed ovary, performed both with and without the use of a transparency technique in the ovarian stromal region.
- It was then possible to view a clear image of the spatial localisation of the follicles and corpus luteum by rotating the reconstructed image of the ovary.
- The researchers effectively extracted images of the follicles and corpus luteum. This offered a quantifiable understanding of their structure, paving the way for further research in this area.
Significance of the Study
- The research enriches scientific knowledge about the internal structure of the equine ovary and its key constituents (the follicles and the corpus luteum), contributing to the fields of equine reproductive science and 3D imaging techniques.
- The findings also open up potential for future studies in 3D imaging and possible medical advancements in equine health treatments through the enhanced understanding of equine internal anatomy.
Cite This Article
APA
Kimura J, Hirano Y, Takemoto S, Nambo Y, Ishinazaka T, Himeno R, Mishima T, Tsumagari S, Yokota H.
(2005).
Three-dimensional reconstruction of the equine ovary.
Anat Histol Embryol, 34(1), 48-51.
https://doi.org/10.1111/j.1439-0264.2004.00567.x Publication
Researcher Affiliations
- College of Bioresource Sciences, Nihon University, Kanagawa, Japan. kimura@brs.nihon-u.ac.jp
MeSH Terms
- Animals
- Female
- Horses / anatomy & histology
- Image Processing, Computer-Assisted
- Imaging, Three-Dimensional / veterinary
- Ovary / anatomy & histology
Citations
This article has been cited 6 times.- Tajika Y, Ishii N, Morimura Y, Fukuda K, Shikada M, Murakami T, Ichinose S, Yoshimoto Y, Iwasaki H. Correlative microscopy and block-face imaging (CoMBI): a 3D imaging method with wide applicability in the field of biological science. Anat Sci Int 2023 Jul;98(3):353-359.
- Ishii N, Tajika Y, Murakami T, Galipon J, Shirahata H, Mukai R, Uehara D, Kaneko R, Yamazaki Y, Yoshimoto Y, Iwasaki H. Correlative microscopy and block-face imaging (CoMBI) method for both paraffin-embedded and frozen specimens. Sci Rep 2021 Jun 23;11(1):13108.
- Hess A, Hinz R, Keliris GA, Boehm-Sturm P. On the Usage of Brain Atlases in Neuroimaging Research. Mol Imaging Biol 2018 Oct;20(5):742-749.
- Tajika Y, Murakami T, Iijima K, Gotoh H, Takahashi-Ikezawa M, Ueno H, Yoshimoto Y, Yorifuji H. A novel imaging method for correlating 2D light microscopic data and 3D volume data based on block-face imaging. Sci Rep 2017 Jun 16;7(1):3645.
- Ono M, Akuzawa H, Nambo Y, Hirano Y, Kimura J, Takemoto S, Nakamura S, Yokota H, Himeno R, Higuchi T, Ohtaki T, Tsumagari S. Analysis of the equine ovarian structure during the first twelve months of life by three-dimensional internal structure microscopy. J Vet Med Sci 2016 Jan;77(12):1599-603.
- Kimura J, Kakusho N, Yamazawa K, Hirano Y, Nambo Y, Yokota H, Himeno R. Stereolithographic biomodeling of equine ovary based on 3D serial digitizing device. J Vet Sci 2009 Jun;10(2):161-3.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
