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Veterinary research communications2000; 24(5); 299-307; doi: 10.1023/a:1006439904937

Tissue culture of the enteric nervous system from equine ileum.

Abstract: Ileal samples were harvested fresh from euthanized adult horses. The tissues were microdissected to prepare wholemount preparations for immunohistochemistry and for either explant or dissociated culture systems of the enteric nervous system. Explant culture systems were established using whole-mounts of either the submucous plexus or the muscularis externa (including the myenteric plexus). Dissociated cell cultures could only be obtained from the submucous plexus. Culture systems were maintained for up to 5 days. Immunoreactivity for a neuronal marker (Pan-N) and for glial cell markers (GFAP and S100) indicated the presence of both neurons and enteric glia in the tissue culture preparations. This is the first report of equine enteric neurons being grown in tissue culture Further refinements to the techniques will be required before this in vitro model can be used for quantitative analysis.
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Publication Date: 2000-06-27 PubMed ID: 10868548DOI: 10.1023/a:1006439904937Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper discusses the process of cultivating cells of the enteric nervous system (ENS) from horse intestines in tissue culture, which is a significant step towards creating a model for more in-depth research on the equine ENS.

Methods

  • The team collected fresh samples of ileum, a part of the intestine, from euthanized adult horses for their experiment.
  • They carefully microdissected these tissues to prepare wholemount preparations needed for both immunohistochemistry, a technique used to visualize specific components of cells, and for developing either explant or dissociated culture systems of the ENS.
  • The researchers used these wholemounts to establish explant culture systems, which involved the cultivation of sectioned tissue pieces, from either the submucous plexus, a network of nerves below the mucosal layer, or the muscularis externa, a layer of muscle that includes the myenteric plexus, another network of nerves.
  • The dissociated cell cultures, where individual cells are separated from each other in the culture, were only obtained from the submucous plexus.
  • The cultures were maintained for up to 5 days.

Results

  • Through immunohistochemistry, the team was able to identify immunoreactivity for a neuronal marker (Pan-N), which can identify the presence of nerve cells, and for glial cell markers (GFAP and S100), which help identify the presence of glial cells—cells in the nervous system that support and protect neurons.
  • This confirmed the presence of both neurons and enteric glia in the tissue culture preparations, thus indicating that the team was successful in growing cells from the equine enteric nervous system in tissue culture.

Conclusion

  • This is the first reported instance of equine enteric neurons being grown in tissue culture, marking a significant advancement in the field.
  • The researchers note that more refinements to the methods will be needed before this in vitro model can be used for quantitative analysis, implying that while this is a substantial step forward, there is still work to be done to enhance the methods’ efficacy.

Cite This Article

APA
Hudson NP, Pearson GT, Mayhew IG. (2000). Tissue culture of the enteric nervous system from equine ileum. Vet Res Commun, 24(5), 299-307. https://doi.org/10.1023/a:1006439904937

Publication

Veterinary research communications
ISSN: 0165-7380
NlmUniqueID: 8100520
Country: Switzerland
Language: English
Volume: 24
Issue: 5
Pages: 299-307

Researcher Affiliations

Hudson, N P
  • Department of Preclinical Veterinary Sciences, Royal (Dick) School of Veterinary Studies, University of Edinburgh, UK.
Pearson, G T
    Mayhew, I G

      MeSH Terms

      • Animals
      • Cell Culture Techniques / methods
      • Cell Culture Techniques / veterinary
      • Culture Techniques / methods
      • Culture Techniques / veterinary
      • Fluorescent Antibody Technique, Indirect
      • Glial Fibrillary Acidic Protein / chemistry
      • Horses / anatomy & histology
      • Horses / physiology
      • Ileum / chemistry
      • Ileum / innervation
      • Ileum / physiology
      • Microscopy, Phase-Contrast / veterinary
      • Neurofilament Proteins / chemistry
      • S100 Proteins / chemistry
      • Submucous Plexus / chemistry
      • Submucous Plexus / cytology
      • Submucous Plexus / physiology

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