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Home / Equine Research Bank / PLoS One / Transcriptional profiling of equine endometrium before, duri...
PloS one2021; 16(10); e0257161; doi: 10.1371/journal.pone.0257161

Transcriptional profiling of equine endometrium before, during and after capsule disintegration during normal pregnancy and after oxytocin-induced luteostasis in non-pregnant mares.

Abstract: The current study used RNA sequencing to determine transcriptional profiles of equine endometrium collected 14, 22, and 28 days after ovulation from pregnant mares. In addition, the transcriptomes of endometrial samples obtained 20 days after ovulation from pregnant mares, and from non-pregnant mares which displayed and failed to display extended luteal function following the administration of oxytocin, were determined and compared in order to delineate genes whose expressions depend on the presence of the conceptus as opposed to elevated progesterone alone. A mere fifty-five transcripts were differentially expressed between samples collected from mares at Day 22 and Day 28 of pregnancy. This likely reflects the longer-term exposure to a relatively constant, progesterone-dominated environment with little change in factors secreted by the conceptus that would affect endometrial gene expression. The complement system was amongst the canonical pathways significantly enriched in transcripts differentially expressed between Day 14 and Day 22/28 of pregnancy. The expression of complement components 7 and 8 was confirmed using in situ hybridization. The expression of SERPING1, an inhibitor of the complement system, was confirmed by immunohistochemistry. In line with the resumed capacity of the endometrium to produce prostaglandin, prostaglandin G/H synthase 1 was expressed at higher levels at Days 22 and 28 than at Day 14 of pregnancy. Our data suggest that this up-regulation is enhanced by the presence of the conceptus; samples obtained from mares at Day 20 of pregnancy had significantly higher levels of prostaglandin G/H synthase 1 transcript than mares with extended luteal function.
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Publication Date: 2021-10-06 PubMed ID: 34614002PubMed Central: PMC8494348DOI: 10.1371/journal.pone.0257161Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article describes an in-depth study on the changes that occur in the endometrium (the lining of the uterus) of mares during pregnancy, as well as in non-pregnant mares following hormonal intervention, through a method called RNA sequencing. This method was used to understand how gene expression varies at different stages of pregnancy.

Methods

  • The study made use of RNA sequencing, a technology that allows for the comprehensive examination of the full range of transcripts in a cell.
  • This technology was utilized to examine the transcriptional profiles of the equine endometrium (the lining of the horse’s uterus) on days 14, 22, and 28 following ovulation in pregnant mares.
  • Additionally, endometrial samples were also collected from mares on the 20th day post-ovulation, both from pregnant mares and non-pregnant mares.
  • A group of non-pregnant mares were also administered oxytocin, a hormone that affects the uterus, to induce extended luteal function, a phase in the menstrual cycle where the uterus prepares for potential pregnancy.

Findings

  • The study found only a small number of differentially expressed genes, only fifty-five to be exact, between days 22 and 28 of pregnancy.
  • This likely indicates that long-term exposure to progesterone, a hormone fundamental to maintaining pregnancy, results in a fairly stable environment within the endometrium that doesn’t affect gene expression to a considerable degree.
  • A significant finding was the enrichment of the complement system pathway among the genes that showed varying expression levels between different stages of pregnancy.
  • Specifically, complement components 7 and 8 showed variation and their expressions were confirmed through method called in situ hybridization which allows for visualization of specific genes within a tissue.
  • The expression of SERPING1, a molecule that inhibits the complement system, was checked and confirmed using immunohistochemistry, a technique for identifying specific molecules within cells or tissues.
  • Prostaglandin G/H synthase 1, an enzyme linked to the production of prostaglandin, was found to be expressed at higher levels on days 22 and 28 of pregnancy as compared to day 14.
  • The researchers posited that this up-regulation might be increased by the presence of the conceptus (the embryo along with its supportive tissues) because samples acquired at day 20 of pregnancy had significantly higher levels of this enzyme’s transcript than non-pregnant mares with extended luteal function.

Conclusion

  • The findings of this study contribute to our understanding of transcriptional changes in the equine endometrium over the course of normal pregnancy and under hormonal influences in non-pregnant conditions.
  • Future studies may build upon these findings to better understand the mechanisms of equine pregnancy, laying the groundwork for potential applications in veterinary medicine and horse breeding practices.

Cite This Article

APA
Klein C, Bruce P, Hammermueller J, Hayes T, Lillie B, Betteridge K. (2021). Transcriptional profiling of equine endometrium before, during and after capsule disintegration during normal pregnancy and after oxytocin-induced luteostasis in non-pregnant mares. PLoS One, 16(10), e0257161. https://doi.org/10.1371/journal.pone.0257161

Publication

PloS one
ISSN: 1932-6203
NlmUniqueID: 101285081
Country: United States
Language: English
Volume: 16
Issue: 10
Pages: e0257161
PII: e0257161

Researcher Affiliations

Klein, Claudia
  • Friedrich-Loeffler-Institute, Institute of Farm Animal Genetics, Mariensee, Germany.
Bruce, Phoebe
  • Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
Hammermueller, Jutta
  • Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
Hayes, Tony
  • Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
Lillie, Brandon
  • Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
Betteridge, Keith
  • Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.

MeSH Terms

  • Animals
  • Corpus Luteum / drug effects
  • Corpus Luteum / physiology
  • Endometrium / metabolism
  • Female
  • Horses / genetics
  • Horses / physiology
  • Ovulation / drug effects
  • Oxytocin / administration & dosage
  • Oxytocin / pharmacology
  • Pregnancy
  • Pregnancy, Animal
  • Transcriptome / drug effects

Conflict of Interest Statement

The authors have declared that no competing interests exist.

References

This article includes 60 references
  1. Bazer FW, Spencer TE, Johnson GA, Burghardt RC, Wu G. Comparative aspects of implantation.. Reproduction 2009 Aug;138(2):195-209.
    doi: 10.1530/REP-09-0158pubmed: 19502456google scholar: lookup
  2. Short RV. Implantation and the maternal recognition of pregnancy. Ciba Foundation Symposium on Foetal Autonomy 1969; London: J&A Churchill.
  3. Bazer FW, Spencer TE, Ott TL. Interferon tau: a novel pregnancy recognition signal.. Am J Reprod Immunol 1997 Jun;37(6):412-20.
    doi: 10.1111/j.1600-0897.1997.tb00253.xpubmed: 9228295google scholar: lookup
  4. Geisert RD, Zavy MT, Moffatt RJ, Blair RM, Yellin T. Embryonic steroids and the establishment of pregnancy in pigs.. J Reprod Fertil Suppl 1990;40:293-305.
    pubmed: 2192045
  5. Ginther OJ. Mobility of the early equine conceptus.. Theriogenology 1983 Apr;19(4):603-11.
    doi: 10.1016/0093-691x(83)90180-2pubmed: 16725808google scholar: lookup
  6. McDowell KJ, Sharp DC, Grubaugh W, Thatcher WW, Wilcox CJ. Restricted conceptus mobility results in failure of pregnancy maintenance in mares.. Biol Reprod 1988 Sep;39(2):340-8.
    doi: 10.1095/biolreprod39.2.340pubmed: 3179385google scholar: lookup
  7. Rivera Del Alamo MM, Reilas T, Kindahl H, Katila T. Mechanisms behind intrauterine device-induced luteal persistence in mares.. Anim Reprod Sci 2008 Aug;107(1-2):94-106.
    doi: 10.1016/j.anireprosci.2007.06.010pubmed: 17643876google scholar: lookup
  8. Wilsher S, Clutton-Brock A, Allen WR. Successful transfer of day 10 horse embryos: influence of donor-recipient asynchrony on embryo development.. Reproduction 2010 Mar;139(3):575-85.
    doi: 10.1530/REP-09-0306pubmed: 19948839google scholar: lookup
  9. Stout TA, Allen WR. Role of prostaglandins in intrauterine migration of the equine conceptus.. Reproduction 2001 May;121(5):771-5.
    pubmed: 11427165
  10. Watson ED, Sertich PL. Prostaglandin production by horse embryos and the effect of co-culture of embryos with endometrium from pregnant mares.. J Reprod Fertil 1989 Sep;87(1):331-6.
    doi: 10.1530/jrf.0.0870331pubmed: 2621704google scholar: lookup
  11. Zavy MT, Vernon MW, Sharp DC 3rd, Bazer FW. Endocrine aspects of early pregnancy in pony mares: a comparison of uterine luminal and peripheral plasma levels of steroids during the estrous cycle and early pregnancy.. Endocrinology 1984 Jul;115(1):214-9.
    doi: 10.1210/endo-115-1-214pubmed: 6734514google scholar: lookup
  12. Raeside JI, Christie HL, Renaud RL, Waelchli RO, Betteridge KJ. Estrogen metabolism in the equine conceptus and endometrium during early pregnancy in relation to estrogen concentrations in yolk-sac fluid.. Biol Reprod 2004 Oct;71(4):1120-7.
    doi: 10.1095/biolreprod.104.028712pubmed: 15163615google scholar: lookup
  13. Stout TA, Allen WR. Prostaglandin E(2) and F(2 alpha) production by equine conceptuses and concentrations in conceptus fluids and uterine flushings recovered from early pregnant and dioestrous mares.. Reproduction 2002 Feb;123(2):261-8.
    pubmed: 11866693
  14. Betteridge KJ. Equine embryology: an inventory of unanswered questions.. Theriogenology 2007 Sep 1;68 Suppl 1:S9-21.
    doi: 10.1016/j.theriogenology.2007.04.037pubmed: 17532037google scholar: lookup
  15. Stout TA, Meadows S, Allen WR. Stage-specific formation of the equine blastocyst capsule is instrumental to hatching and to embryonic survival in vivo.. Anim Reprod Sci 2005 Jul;87(3-4):269-81.
    doi: 10.1016/j.anireprosci.2004.11.009pubmed: 15911176google scholar: lookup
  16. Oriol JG, Sharom FJ, Betteridge KJ. Developmentally regulated changes in the glycoproteins of the equine embryonic capsule.. J Reprod Fertil 1993 Nov;99(2):653-64.
    doi: 10.1530/jrf.0.0990653pubmed: 8107051google scholar: lookup
  17. Arar S, Chan KH, Quinn BA, Waelchli RO, Hayes MA, Betteridge KJ, Monteiro MA. Desialylation of core type 1 O-glycan in the equine embryonic capsule coincides with immobilization of the conceptus in the uterus.. Carbohydr Res 2007 Jun 11;342(8):1110-5.
    doi: 10.1016/j.carres.2007.02.016pubmed: 17335787google scholar: lookup
  18. Betteridge KJ, Waelchli RO, Christie HL, Raeside JI, Quinn BA, Hayes MA. Relationship between the timing of prostaglandin-induced luteolysis and effects on the conceptus during early pregnancy in mares.. Reprod Fertil Dev 2012;24(3):411-24.
    doi: 10.1071/RD11132pubmed: 22401273google scholar: lookup
  19. Kindahl H, Knudsen O, Madej A, Edqvist LE. Progesterone, prostaglandin F-2 alpha, PMSG and oestrone sulphate during early pregnancy in the mare.. J Reprod Fertil Suppl 1982;32:353-9.
    pubmed: 6300390
  20. Douglas RH, Ginther OJ. Concentration of prostaglandins F in uterine venous plasma of anesthetized mares during the estrous cycle and early pregnancy.. Prostaglandins 1976 Feb;11(2):251-60.
    doi: 10.1016/0090-6980(76)90148-9pubmed: 1265294google scholar: lookup
  21. Berglund LA, Sharp DC, Vernon MW, Thatcher WW. Effect of pregnancy and collection technique on prostaglandin F in the uterine lumen of Pony mares.. J Reprod Fertil Suppl 1982;32:335-41.
    pubmed: 6962869
  22. Ealy AD, Eroh ML, Sharp DC 3rd. Prostaglandin H synthase Type 2 is differentially expressed in endometrium based on pregnancy status in pony mares and responds to oxytocin and conceptus secretions in explant culture.. Anim Reprod Sci 2010 Jan;117(1-2):99-105.
    doi: 10.1016/j.anireprosci.2009.03.014pubmed: 19443143google scholar: lookup
  23. Goff AK, Pontbriand D, Sirois J. Oxytocin stimulation of plasma 15-keto-13,14-dihydro prostaglandin F-2 alpha during the oestrous cycle and early pregnancy in the mare.. J Reprod Fertil Suppl 1987;35:253-60.
    pubmed: 3479581
  24. Stout TA, Lamming GE, Allen WR. Oxytocin administration prolongs luteal function in cyclic mares.. J Reprod Fertil 1999 Jul;116(2):315-20.
    doi: 10.1530/jrf.0.1160315pubmed: 10615256google scholar: lookup
  25. Starbuck GR, Stout TA, Lamming GE, Allen WR, Flint AP. Endometrial oxytocin receptor and uterine prostaglandin secretion in mares during the oestrous cycle and early pregnancy.. J Reprod Fertil 1998 Jul;113(2):173-9.
    doi: 10.1530/jrf.0.1130173pubmed: 9861156google scholar: lookup
  26. de Ruijter-Villani M, van Tol HT, Stout TA. Effect of pregnancy on endometrial expression of luteolytic pathway components in the mare.. Reprod Fertil Dev 2015 Jun;27(5):834-45.
    doi: 10.1071/RD13381pubmed: 24679480google scholar: lookup
  27. Merkl M, Ulbrich SE, Otzdorff C, Herbach N, Wanke R, Wolf E, Handler J, Bauersachs S. Microarray analysis of equine endometrium at days 8 and 12 of pregnancy.. Biol Reprod 2010 Nov;83(5):874-86.
    doi: 10.1095/biolreprod.110.085233pubmed: 20631402google scholar: lookup
  28. Klein C, Scoggin KE, Ealy AD, Troedsson MH. Transcriptional profiling of equine endometrium during the time of maternal recognition of pregnancy.. Biol Reprod 2010 Jul;83(1):102-13.
    doi: 10.1095/biolreprod.109.081612pubmed: 20335638google scholar: lookup
  29. Klohonatz KM, Hess AM, Hansen TR, Squires EL, Bouma GJ, Bruemmer JE. Equine endometrial gene expression changes during and after maternal recognition of pregnancy.. J Anim Sci 2015 Jul;93(7):3364-76.
    doi: 10.2527/jas.2014-8826pubmed: 26440005google scholar: lookup
  30. Hayes MA, Quinn BA, Lillie BN, Cote O, Bienzle D, Waelchli RO. Changes in various endometrial proteins during cloprostenol-induced failure of early pregnancy in mares. Animal Reproduction 2012;9(4):723–41.
  31. Stout TA, Troedsson MH. Report of the Havemeyer Foundation Workshop on Equine Implantation: Is early pregnancy loss the only important potential consequence of disturbed preimplantation development?. Equine Vet J 2015 Jul;47(4):381-3.
    doi: 10.1111/evj.12382pubmed: 25973522google scholar: lookup
  32. Betteridge KJ, Raeside JI, Waelchli RO, Christie HL, Hayes MA. Patterns of conceptus development and of progesterone concentrations in maternal blood preceding spontaneous early pregnancy failure in mares.. Reprod Fertil Dev 2018 Jul;30(8):1066-1076.
    doi: 10.1071/RD17336pubmed: 29309736google scholar: lookup
  33. Kenney RM. Prognostic value of endometrial biopsy of the mare.. J Reprod Fertil Suppl 1975 Oct;(23):347-8.
    pubmed: 1060804
  34. Vanderwall DK, Rasmussen DM, Carnahan KG, Davis TL. Effect of administration of oxytocin during diestrus on corpus luteum function and endometrial oxytocin receptor concentration in cycling mares. Journal of Equine Veterinary Science 2012;32(9):536–14.
  35. Altmäe S, Koel M, Võsa U, Adler P, Suhorutšenko M, Laisk-Podar T, Kukushkina V, Saare M, Velthut-Meikas A, Krjutškov K, Aghajanova L, Lalitkumar PG, Gemzell-Danielsson K, Giudice L, Simón C, Salumets A. Meta-signature of human endometrial receptivity: a meta-analysis and validation study of transcriptomic biomarkers.. Sci Rep 2017 Aug 30;7(1):10077.
    doi: 10.1038/s41598-017-10098-3pmc: PMC5577343pubmed: 28855728google scholar: lookup
  36. Klein C, Troedsson M. Equine pre-implantation conceptuses express neuraminidase 2--a potential mechanism for desialylation of the equine capsule.. Reprod Domest Anim 2012 Jun;47(3):449-54.
    doi: 10.1111/j.1439-0531.2011.01901.xpubmed: 22022932google scholar: lookup
  37. Atli MO, Kurar E, Kayis SA, Aslan S, Semacan A, Celik S, Guzeloglu A. Evaluation of genes involved in prostaglandin action in equine endometrium during estrous cycle and early pregnancy.. Anim Reprod Sci 2010 Oct;122(1-2):124-32.
    doi: 10.1016/j.anireprosci.2010.08.007pubmed: 20832957google scholar: lookup
  38. Tai HH, Ensor CM, Tong M, Zhou H, Yan F. Prostaglandin catabolizing enzymes.. Prostaglandins Other Lipid Mediat 2002 Aug;68-69:483-93.
    doi: 10.1016/s0090-6980(02)00050-3pubmed: 12432938google scholar: lookup
  39. Stewart F, Gerstenberg C, Suire S, Allen WR. Immunolocalization of a novel protein (P19) in the endometrium of fertile and subfertile mares.. J Reprod Fertil Suppl 2000;(56):593-9.
    pubmed: 20681174
  40. Ellenberger C, Wilsher S, Allen WR, Hoffmann C, Kölling M, Bazer FW, Klug J, Schoon D, Schoon HA. Immunolocalisation of the uterine secretory proteins uterocalin, uteroferrin and uteroglobin in the mare's uterus and placenta throughout pregnancy.. Theriogenology 2008 Sep 15;70(5):746-57.
    doi: 10.1016/j.theriogenology.2008.04.050pubmed: 18547636google scholar: lookup
  41. Song G, Bazer FW, Wagner GF, Spencer TE. Stanniocalcin (STC) in the endometrial glands of the ovine uterus: regulation by progesterone and placental hormones.. Biol Reprod 2006 May;74(5):913-22.
    doi: 10.1095/biolreprod.106.050807pubmed: 16452456google scholar: lookup
  42. Song G, Dunlap KA, Kim J, Bailey DW, Spencer TE, Burghardt RC, Wagner GF, Johnson GA, Bazer FW. Stanniocalcin 1 is a luminal epithelial marker for implantation in pigs regulated by progesterone and estradiol.. Endocrinology 2009 Feb;150(2):936-45.
    doi: 10.1210/en.2008-1026pubmed: 18845628google scholar: lookup
  43. Klein C. The role of relaxin in mare reproductive physiology: A comparative review with other species.. Theriogenology 2016 Jul 1;86(1):451-6.
    doi: 10.1016/j.theriogenology.2016.04.061pubmed: 27158127google scholar: lookup
  44. de Ruijter-Villani M, Deelen C, Stout TA. Expression of leukaemia inhibitory factor at the conceptus?maternal interface during preimplantation development and in the endometrium during the oestrous cycle in the mare.. Reprod Fertil Dev 2015 Apr 17;.
    doi: 10.1071/RD14334pubmed: 25881292google scholar: lookup
  45. Petraglia F, Florio P, Luisi S, Gallo R, Gadducci A, Viganò P, Di Blasio AM, Genazzani AR, Vale W. Expression and secretion of inhibin and activin in normal and neoplastic uterine tissues. High levels of serum activin A in women with endometrial and cervical carcinoma.. J Clin Endocrinol Metab 1998 Apr;83(4):1194-200.
    doi: 10.1210/jcem.83.4.4689pubmed: 9543140google scholar: lookup
  46. Yamanouchi K, Hirasawa K, Hasegawa T, Ikeda A, Chang KT, Matsuyama S, Nishihara M, Miyazawa K, Sawasaki T, Tojo H, Tachi C, Takahashi M. Equine inhibin/activin beta A-subunit mRNA is expressed in the endometrial gland, but not in the trophoblast, during pregnancy.. Mol Reprod Dev 1997 Aug;47(4):363-9.
    pubmed: 9211420doi: 10.1002/(sici)1098-2795(199708)47:4<363::aid-mrd2>3.0.co;2-igoogle scholar: lookup
  47. Kimura Y, Sasaki M, Watanabe K, Dhakal P, Sato F, Taya K, Nambo Y. Expression of activin receptors in the equine uteroplacental tissue: an immunohistochemical analysis.. J Equine Sci 2018;29(2):33-37.
    doi: 10.1294/jes.29.33pmc: PMC6033615pubmed: 29991920google scholar: lookup
  48. Walker CG, Meier S, Littlejohn MD, Lehnert K, Roche JR, Mitchell MD. Modulation of the maternal immune system by the pre-implantation embryo.. BMC Genomics 2010 Aug 13;11:474.
    doi: 10.1186/1471-2164-11-474pmc: PMC3091670pubmed: 20707927google scholar: lookup
  49. Palomino WA, Argandoña F, Azúa R, Kohen P, Devoto L. Complement C3 and decay-accelerating factor expression levels are modulated by human chorionic gonadotropin in endometrial compartments during the implantation window.. Reprod Sci 2013 Sep;20(9):1103-10.
    doi: 10.1177/1933719113477486pmc: PMC3745714pubmed: 23427180google scholar: lookup
  50. Francis J, Rai R, Sebire NJ, El-Gaddal S, Fernandes MS, Jindal P, Lokugamage A, Regan L, Brosens JJ. Impaired expression of endometrial differentiation markers and complement regulatory proteins in patients with recurrent pregnancy loss associated with antiphospholipid syndrome.. Mol Hum Reprod 2006 Jul;12(7):435-42.
    doi: 10.1093/molehr/gal048pubmed: 16735457google scholar: lookup
  51. Kaul A, Nagamani M, Nowicki B. Decreased expression of endometrial decay accelerating factor (DAF), a complement regulatory protein, in patients with luteal phase defect.. Am J Reprod Immunol 1995 Oct;34(4):236-40.
    doi: 10.1111/j.1600-0897.1995.tb00947.xpubmed: 8579761google scholar: lookup
  52. Palomino WA, Tayade C, Argandoña F, Devoto L, Young SL, Lessey BA. The endometria of women with endometriosis exhibit dysfunctional expression of complement regulatory proteins during the mid secretory phase.. J Reprod Immunol 2018 Feb;125:1-7.
    doi: 10.1016/j.jri.2017.10.046pubmed: 29153978google scholar: lookup
  53. Beinrohr L, Murray-Rust TA, Dyksterhuis L, Závodszky P, Gál P, Pike RN, Wijeyewickrema LC. Serpins and the complement system.. Methods Enzymol 2011;499:55-75.
    doi: 10.1016/B978-0-12-386471-0.00004-3pubmed: 21683249google scholar: lookup
  54. Grant DM, Macedo A, Toms D, Klein C. Fibrinogen in equine pregnancy as a mediator of cell adhesion, an epigenetic and functional investigation.. Biol Reprod 2020 Feb 12;102(1):170-184.
    doi: 10.1093/biolre/ioz157pubmed: 31403677google scholar: lookup
  55. Rijken DC, Lijnen HR. New insights into the molecular mechanisms of the fibrinolytic system.. J Thromb Haemost 2009 Jan;7(1):4-13.
    doi: 10.1111/j.1538-7836.2008.03220.xpubmed: 19017261google scholar: lookup
  56. Brown EW, Ravindran S, Patston PA. The reaction between plasmin and C1-inhibitor results in plasmin inhibition by the serpin mechanism.. Blood Coagul Fibrinolysis 2002 Dec;13(8):711-4.
    doi: 10.1097/00001721-200212000-00007pubmed: 12441910google scholar: lookup
  57. Ganz T, Selsted ME, Lehrer RI. Defensins.. Eur J Haematol 1990 Jan;44(1):1-8.
    doi: 10.1111/j.1600-0609.1990.tb00339.xpubmed: 2407547google scholar: lookup
  58. Schoniger S, Grafe H, Schoon HA. Beta-defensin is a component of the endometrial immune defence in the mare. Pferdeheilkunde 2013;29(3):335–46.
  59. Johnson GA, Burghardt RC, Bazer FW. Osteopontin: a leading candidate adhesion molecule for implantation in pigs and sheep.. J Anim Sci Biotechnol 2014;5(1):56.
    doi: 10.1186/2049-1891-5-56pmc: PMC4322467pubmed: 25671104google scholar: lookup
  60. Klein C, Troedsson MH. Transcriptional profiling of equine conceptuses reveals new aspects of embryo-maternal communication in the horse.. Biol Reprod 2011 May;84(5):872-85.
    doi: 10.1095/biolreprod.110.088732pubmed: 21209420google scholar: lookup

Citations

This article has been cited 1 times.
  1. Rudolf Vegas A, Hamdi M, Podico G, Bollwein H, Fröhlich T, Canisso IF, Bauersachs S, Almiñana C. Uterine extracellular vesicles as multi-signal messengers during maternal recognition of pregnancy in the mare.. Sci Rep 2022 Sep 16;12(1):15616.
    doi: 10.1038/s41598-022-19958-zpubmed: 36114358google scholar: lookup
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