Tumour suppressor gene p53 in the horse: identification, cloning, sequencing and a possible role in the pathogenesis of equine sarcoid.
Abstract: The tumour suppressor protein p53 enhances the genetic stability of the cell and plays a critical role in tumour suppression. Equine p53 was analysed by sequencing exons 5 to 9, a region which includes most known mutations and all the mutational hotspots in the species that have been investigated. The fragment was amplified, cloned and sequenced from genomic and complementary DNA. A comparison of the predicted amino acid sequences between the horse and other species resulted in identities between 66 per cent with the clawed frog and 92 per cent with the cat. Using the single strand conformation polymorphism technique, exons 5 to 8 amplified from sarcoid tissue and peripheral leucocytes of 28 sarcoid-affected and 11 healthy horses were screened for mutations. No mutations were identified, suggesting that the frequency of p53 mutations in equine sarcoid might be low. However, the high incidence of bovine papillomavirus (BPV) infection in equine sarcoid may indicate the functional inactivation of p53 by BPV-encoded E6 protein.
Publication Date: 1996-09-01 PubMed ID: 8880979DOI: 10.1016/s0034-5288(96)90084-3Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research looks at the role of p53, a gene that suppresses tumors, in horses. By cloning and sequencing exons 5 to 9 of this gene, the scientists attempted to identify its role in equine sarcoid, a common skin tumor in horses. The results, although inconclusive, indicate that p53 may be functionally inactivated by bovine papillomavirus, a common infection in equine sarcoid.
Understanding the Role of p53
- At the heart of this research is the p53 gene, which is known to suppress tumors. It plays a critical role in maintaining the genetic stability of cells.
- The researchers investigated this gene in horses, specifically looking at exons 5 to 9. This is because this region in the gene contains most known mutations and all the known mutational hotspots.
Research Methods
- The research team examined this region of the gene by amplifying, cloning, and sequencing it from both genomic and complementary DNA.
- They then compared the predicted amino acid sequences between horses and other species. These comparative analyses ranged from a 66 per cent similarity with the clawed frog to 92 per cent similarity with cats.
Investigation of the Gene in Sarcoid-affected and Healthy Horses
- The researchers screened for mutations in exons 5 to 8 in both sarcoid tissue and peripheral leucocytes from 28 sarcoid-affected and 11 healthy horses. They used the single strand conformation polymorphism technique.
- Despite their efforts, they identified no mutations. This could mean that the frequency of p53 mutations in equine sarcoid is low.
Impact of Bovine Papillomavirus
- A significant finding was the high incidence of bovine papillomavirus (BPV) infection in equine sarcoid.
- The researchers hypothesize that p53 may be functionally inactivated by the E6 protein, which is encoded by BPV. This means that although no mutations were found in p53, the gene may not function properly due to this viral interference, thereby potentially contributing to the development of equine sarcoid.
Cite This Article
APA
Bucher K, Szalai G, Marti E, Griot-Wenk ME, Lazary S, Pauli U.
(1996).
Tumour suppressor gene p53 in the horse: identification, cloning, sequencing and a possible role in the pathogenesis of equine sarcoid.
Res Vet Sci, 61(2), 114-119.
https://doi.org/10.1016/s0034-5288(96)90084-3 Publication
Researcher Affiliations
- Division of Immunogenetics, University of Berne, Switzerland.
MeSH Terms
- Amino Acid Sequence
- Animals
- Base Sequence
- Cats
- Cattle
- Chickens
- Chlorocebus aethiops
- Cloning, Molecular
- Cricetinae
- DNA Primers
- Exons
- Genes, p53
- Horse Diseases
- Horses / genetics
- Humans
- Mice
- Molecular Sequence Data
- Polymerase Chain Reaction
- Rats
- Sarcoma / genetics
- Sarcoma / veterinary
- Sequence Homology, Amino Acid
- Sheep
- Skin Neoplasms / genetics
- Skin Neoplasms / veterinary
- Trout
- Tumor Suppressor Protein p53 / biosynthesis
- Tumor Suppressor Protein p53 / chemistry
- Tumor Suppressor Protein p53 / genetics
- Xenopus
Citations
This article has been cited 3 times.- Tura G, Brunetti B, Brigandì E, Rinnovati R, Sarli G, Avallone G, Muscatello LV, La Ragione RM, Durham AE, Bacci B. Expression of Cell-Cycle Regulatory Proteins pRb, Cyclin D1, and p53 Is Not Associated with Recurrence Rates of Equine Sarcoids.. Vet Sci 2022 Sep 1;9(9).
- Martano M, Power K, Restucci B, Pagano I, Altamura G, Borzacchiello G, Maiolino P. Expression of vascular endothelial growth factor (VEGF) in equine sarcoid.. BMC Vet Res 2018 Sep 3;14(1):266.
- Finlay M, Yuan Z, Morgan IM, Campo MS, Nasir L. Equine sarcoids: Bovine Papillomavirus type 1 transformed fibroblasts are sensitive to cisplatin and UVB induced apoptosis and show aberrant expression of p53.. Vet Res 2012 Dec 4;43(1):81.
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