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Home / Equine Research Bank / Transbound Emerg Dis / Use of nanopore sequencing to characterize african horse sic...
Transboundary and emerging diseases2021; 69(3); 1010-1019; doi: 10.1111/tbed.14056

Use of nanopore sequencing to characterize african horse sickness virus (AHSV) from the African horse sickness outbreak in thailand in 2020.

Abstract: African horse sickness (AHS) is a highly infectious and deadly disease despite availability of vaccines. Molecular characterization of African horse sickness virus (AHSV) detected from the March 2020 Thailand outbreak was carried out by whole-genome sequencing using Nanopore with a Sequence-Independent Single Primer Amplification (SISPA) approach. Nucleotide sequence of the whole genome was compared with closest matching AHSV strains using phylogenetic analyses and the AHSV-1 virus shared high sequence identity with isolates from the same outbreak. Substitution analysis revealed non-synonymous and synonymous substitutions in the VP2 gene as compared to circulating South African strains. The use of sequencing technologies, such as Nanopore with SISPA, has enabled rapid detection, identification and detailed genetic characterization of the AHS virus for informed decision-making and implementation of disease control measures. Active genetic information sharing has also allowed emergence of AHSV to be better monitored on a global basis.
© 2021 The Authors. Transboundary and Emerging Diseases published by Wiley-VCH GmbH.
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Publication Date: 2021-03-30 PubMed ID: 33682298DOI: 10.1111/tbed.14056Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research study investigates African horse sickness, a severe disease, by using advanced genomic sequencing techniques. The study offers a detailed genetic analysis of the virus that caused the 2020 outbreak in Thailand, providing critical insights for future disease control measures.

Research Study Overview

  • The research focuses on African horse sickness (AHS), a highly lethal and contagious disease that affects horses. Despite the availability of vaccines, the disease remains a significant threat.
  • The researchers used advanced genomic sequencing techniques to analyze samples from a 2020 outbreak of AHS in Thailand.
  • Nanopore sequencing and a method called Sequence-Independent Single Primer Amplification (SISPA) were used to carry out whole-genome sequencing of the African horse sickness virus (AHSV).

Findings of the Study

  • The whole-genome sequencing allowed the researchers to compare the nucleotide sequence of the entire genome of the AHSV identified in Thailand with existing strains of the virus.
  • Phylogenetic analysis, a method used to depict the evolutionary relationships between species, revealed that AHSV-1 virus from the Thailand outbreak shared a high level of genetic similarity with other isolates from the same breakout.
  • A detailed substitution analysis, which studied changes in the genetic code, showed both non-synonymous and synonymous substitutions in a critical gene called VP2 when compared to the strains common in South Africa. Non-synonymous substitutions are changes that affect protein sequence, while synonymous substitutions do not.

Implications of the Research

  • This study provided detailed genetic characterization of the AHSV responsible for the recent outbreak in Thailand, which is crucial information for the development and implementation of disease control measures.
  • The use of advanced sequencing technologies like Nanopore with SISPA has made it possible to quickly identify and examine the genetics of the virus in detail.
  • Sharing the genetic information of AHSV can improve global monitoring of the virus. It can help in predicting and responding to future outbreaks by understanding the changes and trends in the disease’s evolution.

Cite This Article

APA
Toh X, Wang Y, Rajapakse MP, Lee B, Songkasupa T, Suwankitwat N, Kamlangdee A, Judith Fernandez C, Huangfu T. (2021). Use of nanopore sequencing to characterize african horse sickness virus (AHSV) from the African horse sickness outbreak in thailand in 2020. Transbound Emerg Dis, 69(3), 1010-1019. https://doi.org/10.1111/tbed.14056

Publication

Transboundary and emerging diseases
ISSN: 1865-1682
NlmUniqueID: 101319538
Country: Germany
Language: English
Volume: 69
Issue: 3
Pages: 1010-1019

Researcher Affiliations

Toh, Xinyu
  • Center for Animal & Veterinary Sciences, Professional and Scientific Services, Animal and Veterinary Service, National Parks Board (NParks), Singapore.
Wang, Yifan
  • Center for Animal & Veterinary Sciences, Professional and Scientific Services, Animal and Veterinary Service, National Parks Board (NParks), Singapore.
Rajapakse, Menaka Priyadharsani
  • Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore.
Lee, Bernett
  • Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore.
Songkasupa, Tapanut
  • Virology section, Department of Livestock Development, National Institute of Animal Health, Bangkok, Thailand.
Suwankitwat, Nutthakarn
  • Virology section, Department of Livestock Development, National Institute of Animal Health, Bangkok, Thailand.
Kamlangdee, Attapon
  • Faculty of Veterinary Medicine, Kasetsart university, Kamphaengsean, Thailand.
Judith Fernandez, Charlene
  • Center for Animal & Veterinary Sciences, Professional and Scientific Services, Animal and Veterinary Service, National Parks Board (NParks), Singapore.
Huangfu, Taoqi
  • Center for Animal & Veterinary Sciences, Professional and Scientific Services, Animal and Veterinary Service, National Parks Board (NParks), Singapore.

MeSH Terms

  • African Horse Sickness
  • African Horse Sickness Virus
  • Animals
  • Disease Outbreaks / veterinary
  • Horse Diseases / epidemiology
  • Horses
  • Nanopore Sequencing / veterinary
  • Phylogeny
  • Thailand / epidemiology
  • Viral Vaccines

Grant Funding

  • National Parks Board - Singapore

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