Validation of a point-of-care polymerase chain reaction assay for detection of Streptococcus equi subspecies equi in rostral nasal swabs from horses with suspected strangles.
Abstract: This study aimed to validate a point-of-care polymerase chain reaction (PCR) assay for detection of subsp. in rostral nasal swabs from horses with suspected acute strangles and to compare the results against the molecular gold standard of quantitative polymerase chain reaction (qPCR). Two hundred thirty-two individual swabs of rostral nasal passages were characterized by qPCR as positive, subsp. positive, or and negative. The specificity and sensitivity of the point-of-care PCR assay were 89% and 84%, respectively. The limits of detection of the qPCR assay and the point-of-care PCR analyzer were 3 and 277 target genes of , respectively. Overall agreement and short turnaround time make the point-of-care PCR assay a potential molecular diagnostic platform that will enhance the capability of equine veterinarians to timely support a diagnosis of strangles and institute proper biosecurity protocols. . La présente étude visait à valider une épreuve d’amplification en chaîne par la polymérase (PCR) au point de service pour la détection de ssp. () à partir d’écouvillons nasaux rostraux de chevaux suspectés être atteints de gourme aiguë et de comparer les résultats à ceux de l’épreuve étalon de la réaction d’amplification en chaîne par la polymérase quantitative (qPCR). Deux cent trente-deux écouvillons individuels des voies nasales rostrales furent caractérisés par qPCR comme étant positif, ssp. () positif ou et négatifs. La spécificité et la sensibilité de l’épreuve PCR au point de service étaient de 89 % et 84 %, respectivement. Les limites de détection de l’épreuve par qPCR et de l’analyseur PCR au point de service étaient de 3 et 277 copies du gène cible de , respectivement. L’accord général et le court temps de réponse font du PCR au point de service une plate-forme de diagnostic moléculaire potentielle qui augmentera les capacités des vétérinaires équins à appuyer adéquatement un diagnostic de gourme et d’instituer les protocoles de biosécurité appropriés.(Traduit par D Serge Messier).
Copyright and/or publishing rights held by the Canadian Veterinary Medical Association.
Publication Date: 2021-01-05 PubMed ID: 33390599PubMed Central: PMC7739398
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- Journal Article
- Biosecurity
- Clinical Study
- Comparative Study
- Diagnosis
- Diagnostic Technique
- Disease
- Disease Diagnosis
- Equine Health
- Horses
- Infection
- Infectious Disease
- Molecular biology
- Nasal
- Polymerase Chain Reaction
- Sensitivity and Specificity
- Strangles
- Streptococcus
- Veterinary Care
- Veterinary Medicine
- Veterinary Procedure
- Veterinary Research
Summary
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The research validates a point-of-care PCR (Polymerase Chain Reaction) method for detecting Streptococcus equi species in horses suspected of having strangles, an infectious equine disease. The new method’s performance is compared against the golden standard quantitative PCR (qPCR) method.
Objective of the Research
- The research sought to validate a point-of-care PCR assay for detecting Streptococcus equi subspecies equi (a bacterium causing an infectious disease known as strangles in horses) from nasal swabs.
- It also aimed to compare the effectiveness of this newly proposed method with the established molecular gold standard, known as quantitative PCR (qPCR).
Methodology
- As part of the study, 232 individual nasal swabs from horses suspected of having acute strangles were tested.
- The results of the tests (i.e., whether or not they detected the bacterium) were then analyzed and classified as positive or negative.
Findings
- Overall, the point-of-care PCR method showed a specificity and sensitivity of 89% and 84% respectively, indicating it is quite accurate.
- The point-of-care PCR had a limit of detecting 277 Streptococcus equi target genes, compared to the qPCR limit of 3.
Implications of the Research
- The research suggests that this point-of-care PCR test could potentially be used as a new diagnostic platform in identifying strangles.
- A key advantage of the new method is its short turnaround time, which could enhance equine veterinarians’ capability to confirm a diagnosis and implement necessary biosecurity protocols in a timely manner.
Cite This Article
APA
Willis AT, Barnum S, Pusterla N.
(2021).
Validation of a point-of-care polymerase chain reaction assay for detection of Streptococcus equi subspecies equi in rostral nasal swabs from horses with suspected strangles.
Can Vet J, 62(1), 51-54.
Publication
Researcher Affiliations
- William R. Pritchard Veterinary Medical Teaching Hospital, School of Veterinary Medicine, University of California, 1 Garrod Drive, Davis, California, USA (Willis); Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, One Shields Avenue, Davis, California, USA (Barnum, Pusterla).
- William R. Pritchard Veterinary Medical Teaching Hospital, School of Veterinary Medicine, University of California, 1 Garrod Drive, Davis, California, USA (Willis); Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, One Shields Avenue, Davis, California, USA (Barnum, Pusterla).
- William R. Pritchard Veterinary Medical Teaching Hospital, School of Veterinary Medicine, University of California, 1 Garrod Drive, Davis, California, USA (Willis); Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, One Shields Avenue, Davis, California, USA (Barnum, Pusterla).
MeSH Terms
- Animals
- Horse Diseases / diagnosis
- Horses
- Point-of-Care Systems
- Polymerase Chain Reaction / veterinary
- Streptococcal Infections / diagnosis
- Streptococcal Infections / veterinary
- Streptococcus
- Streptococcus equi / genetics
References
This article includes 5 references
- Sweeney CR, Timoney JF, Newton JR, Hines MT. Streptococcus equi infections in horses: guidelines for treatment, control, and prevention of strangles.. J Vet Intern Med 2005 Jan-Feb;19(1):123-34.
- Boyle AG, Timoney JF, Newton JR, Hines MT, Waller AS, Buchanan BR. Streptococcus equi Infections in Horses: Guidelines for Treatment, Control, and Prevention of Strangles-Revised Consensus Statement.. J Vet Intern Med 2018 Mar;32(2):633-647.
- Waller AS. New perspectives for the diagnosis, control, treatment, and prevention of strangles in horses.. Vet Clin North Am Equine Pract 2014 Dec;30(3):591-607.
- Basile K, Kok J, Dwyer DE. Point-of-care diagnostics for respiratory viral infections.. Expert Rev Mol Diagn 2018 Jan;18(1):75-83.
- Pusterla N, Leutenegger CM, Barnum SM, Byrne BA. Use of quantitative real-time PCR to determine viability of Streptococcus equi subspecies equi in respiratory secretions from horses with strangles.. Equine Vet J 2018 Sep;50(5):697-700.
Citations
This article has been cited 1 times.- Adewusi OO, Waldner CL, Hanington PC, Hill JE, Freeman CN, Otto SJG. Laboratory tools for the direct detection of bacterial respiratory infections and antimicrobial resistance: a scoping review. J Vet Diagn Invest 2024 May;36(3):400-417.
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