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Home / Equine Research Bank / Vet World / Vancomycin resistant Streptococcus equi subsp. equi isolated...
Veterinary world2021; 14(7); 1808-1814; doi: 10.14202/vetworld.2021.1808-1814

Vancomycin resistant Streptococcus equi subsp. equi isolated from equines suffering from respiratory manifestation in Egypt.

Abstract: Upper respiratory tract infections are common in horses and can be caused by a variety of pathogens, mainly subsp, which are a significant equine pathogen causing major health issues as well as financial losses to the equine industry. This study aimed to determine the prevalence of bacteria in equines in Egypt, and characterize vancomycin-resistant subsp phenotypically and genotypically. Methods: subsp was isolated from internal nares of horses. All strains were confirmed by polymerase chain reaction-based detection of genus-specific , A and I genes. Antibiotic susceptibility was determined phenotypically using the disk diffusion method. Genotypic detection of antibiotic resistance genes was performed by analyzing as b-lactamase resistance (Z), tetracycline resistance (K), vancomycin resistance (A), and chloramphenicol resistance (A). Results: Eight streptococcal isolates were confirmed as . subsp. The genotypic characterization of antibiotic resistance showed resistance to A and K, with a frequency of 87.5% and 12.5%, respectively, while the frequency of sensitivity was 100% for gene and A gene. Conclusions: In this study, we assessed vancomycin-resistant subsp from equines suffering from respiratory manifestation in Egypt.
Copyright: © Arafa, et al.
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Publication Date: 2021-07-14 PubMed ID: 34475702PubMed Central: PMC8404119DOI: 10.14202/vetworld.2021.1808-1814Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research study explores the prevalence and properties of vancomycin-resistant Streptococcus equi subsp. equi, a bacterium that causes upper respiratory tract infections in horses, in Egypt.

Study Purpose and Methodology

  • The main goal of this research was to identify the prevalence of Streptococcus equi subsp. equi (S. equi) in horses in Egypt and to further analyze any vancomycin-resistant strains both phenotypically and genotypically.
  • S. equi was collected from the internal noses of horses for examination. The identity of these strains was then confirmed using polymerase chain reaction (PCR), a method of replicating DNA in a lab to create enough for analysis. Here, it was used to detect specific S. equi genes.
  • Antibiotic susceptibility was gauged phenotypically by the disk diffusion method, which involves placing a disk soaked in antibiotics on a culture of the bacteria to see how effectively it inhibits bacterial growth.
  • The exploration for antibiotic resistance genes was performed genotypically by seeking genes that facilitate resistance to b-lactamase (Z), tetracycline (K), vancomycin (A), and chloramphenicol (A).

Study Results

  • In the course of the investigation, eight isolates were identified as S. equi subsp. equi.
  • The genotypic characterization revealed resistance to tetracycline (K) with a frequency of 12.5% and vancomycin (A) with 87.5% frequency among the isolates.
  • The frequency of sensitivity was found to be 100% for the b-lactamase gene and chloramphenicol gene.

Study Conclusions

  • This research succeeded in assessing vancomycin-resistant S. equi from horses in Egypt suffering from respiratory conditions.
  • The high prevalence of vancomycin resistance is notable, particularly given the role of S. equi in causing significant health and financial impacts to the equine industry.

Cite This Article

APA
Arafa AA, Hedia RH, Ata NS, Ibrahim ES. (2021). Vancomycin resistant Streptococcus equi subsp. equi isolated from equines suffering from respiratory manifestation in Egypt. Vet World, 14(7), 1808-1814. https://doi.org/10.14202/vetworld.2021.1808-1814

Publication

Veterinary world
ISSN: 0972-8988
NlmUniqueID: 101504872
Country: India
Language: English
Volume: 14
Issue: 7
Pages: 1808-1814

Researcher Affiliations

Arafa, Amany A
  • Department of Microbiology and Immunology, National Research Centre, Dokki, Egypt.
Hedia, Riham H
  • Department of Microbiology and Immunology, National Research Centre, Dokki, Egypt.
Ata, Nagwa S
  • Department of Microbiology and Immunology, National Research Centre, Dokki, Egypt.
Ibrahim, Eman S
  • Department of Microbiology and Immunology, National Research Centre, Dokki, Egypt.

References

This article includes 40 references
  1. Paillot R, Darby AC, Robinson C, Wright NL, Steward KF, Anderson E, Webb K, Holden MT, Efstratiou A, Broughton K, Jolley KA, Priestnall SL, Marotti Campi MC, Hughes MA, Radford A, Erles K, Waller AS. Identification of three novel superantigen-encoding genes in Streptococcus equi subsp. zooepidemicus, szeF, szeN, and szeP.. Infect Immun 2010 Nov;78(11):4817-27.
    pmc: PMC2976332pubmed: 20713629doi: 10.1128/iai.00751-10google scholar: lookup
  2. Mallicote M. Update on Streptococcus equi subsp equi infections.. Vet Clin North Am Equine Pract 2015 Apr;31(1):27-41.
    pubmed: 25600455doi: 10.1016/j.cveq.2014.11.003google scholar: lookup
  3. Mani RJ, Thachil AJ, Ramachandran A. Discrimination of Streptococcus equi subsp. equi and Streptococcus equi subsp. zooepidemicus using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.. J Vet Diagn Invest 2017 Sep;29(5):622-627.
    pubmed: 28460559doi: 10.1177/1040638717702687google scholar: lookup
  4. Boyle AG. Strangles and its complications.. Equine Vet. Educ. 2017;29(1):149–157.
  5. Torpiano P, Nestorova N, Vella C. Streptococcus equi subsp. equi meningitis, septicemia and subdural empyema in a child.. IDCases 2020;21:e00808.
    pmc: PMC7256365pubmed: 32489871doi: 10.1016/j.idcr.2020.e00808google scholar: lookup
  6. Mohamed OM, Abo El-Hassan DG, El-Molla AA. Study on b-haemolytic Streptococci infection in equines at different seasons and ages.. Egypt. J. Vet. Sci. 2018;49(2):147–154.
  7. Reed MS, Bayly MW, Sellon CD. Equine Internal Medicine.. 2nd ed. United States: Saunders; 2004. pp. 59–68.
  8. Al Seady MM, Atwa SM, El Beskawy MA, Elkhodary SA, Eissa MI, Ibrahim NA. Clinical molecular diagnosis and antimicrobial sensitivity of Streptococcus equi causing strangle.. Alex. J. Vet. Sci. 2018;56(2):78–83.
  9. Boyle AG, Timoney JF, Newton JR, Hines MT, Waller AS, Buchanan BR. Streptococcus equi Infections in Horses: Guidelines for Treatment, Control, and Prevention of Strangles-Revised Consensus Statement.. J Vet Intern Med 2018 Mar;32(2):633-647.
    pmc: PMC5867011pubmed: 29424487doi: 10.1111/jvim.15043google scholar: lookup
  10. Salmon SA, Watts JL, Yancey RJ Jr. In vitro activity of ceftiofur and its primary metabolite, desfuroylceftiofur, against organisms of veterinary importance.. J Vet Diagn Invest 1996 Jul;8(3):332-6.
    pubmed: 8844576doi: 10.1177/104063879600800309google scholar: lookup
  11. Watanakunakorn C. The antibacterial action of vancomycin.. Rev Infect Dis 1981 Nov-Dec;3 suppl:S210-5.
    pubmed: 6805065
  12. Quinn PJ, Markey BK, Leonard FC, FitzPatrick ES, Fanning S, Hartigan PJ. Veterinary Microbiology and Microbial Diseases.. 2nd ed. Hoboken, New Jersey: Wiley-Blackwell, John Wiley and Sons, Ltd; 2011.
  13. World Health Organization. Critically Important Antimicrobials for Human Medicine.. 5th ed. Geneva: World Health Organization; 2017.
  14. Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing.. 30th ed. Pennsylvania, PA: Clinical and Laboratory Standards Institute; 2020.
  15. Osakabe Y, Yaguchi C, Miyai T, Miyata K, Mineo S, Nakamura M, Amano S. Detection of Streptococcus species by polymerase chain reaction in infectious crystalline keratopathy.. Cornea 2006 Dec;25(10):1227-30.
    pubmed: 17172904doi: 10.1097/01.ico.0000240083.31432.70google scholar: lookup
  16. Preziuso S, Cuteri V. A multiplex polymerase chain reaction assay for direct detection and differentiation of β-hemolytic streptococci in clinical samples from horses.. JEVS 2012;32(5):292–296.
  17. Bagcigil AF, Taponen S, Koort J, Bengtsson B, Myllyniemi AL, Pyörälä S. Genetic basis of penicillin resistance of S. aureus isolated in bovine mastitis.. Acta Vet Scand 2012 Nov 23;54(1):69.
    pmc: PMC3575348pubmed: 23176642doi: 10.1186/1751-0147-54-69google scholar: lookup
  18. Patel R, Uhl JR, Kohner P, Hopkins MK, Cockerill FR 3rd. Multiplex PCR detection of vanA, vanB, vanC-1, and vanC-2/3 genes in enterococci.. J Clin Microbiol 1997 Mar;35(3):703-7.
    pmc: PMC229654pubmed: 9041416doi: 10.1128/jcm.35.3.703-707.1997google scholar: lookup
  19. Kehrenberg C, Schwarz S. Distribution of florfenicol resistance genes fexA and cfr among chloramphenicol-resistant Staphylococcus isolates.. Antimicrob Agents Chemother 2006 Apr;50(4):1156-63.
    pmc: PMC1426988pubmed: 16569824doi: 10.1128/aac.50.4.1156-1163.2006google scholar: lookup
  20. Zellweger RM, Carrique-Mas J, Limmathurotsakul D, Day NPJ, Thwaites GE, Baker S. A current perspective on antimicrobial resistance in Southeast Asia.. J Antimicrob Chemother 2017 Nov 1;72(11):2963-2972.
    pmc: PMC5890732pubmed: 28961709doi: 10.1093/jac/dkx260google scholar: lookup
  21. Rousham EK, Unicomb L, Islam MA. Human, animal and environmental contributors to antibiotic resistance in low-resource settings: integrating behavioural, epidemiological and One Health approaches.. Proc Biol Sci 2018 Apr 11;285(1876).
    pmc: PMC5904322pubmed: 29643217doi: 10.1098/rspb.2018.0332google scholar: lookup
  22. Lindahl S. Streptococcus equi subsp.equi and Streptococcus equi subsp. zooepidemicus. Upper Respiratory Disease in Horses and Zoonotic Transmission to Humans.. Acta Universitatis Agriculturae Sueciae Doctoral Thesis Swedish University of Agricultural Sciences Uppsala 2013. No. 53. 2013.
  23. Delph KM, Beard LA, Trimble AC, Sutter ME, Timoney JF, Morrow JK. Strangles, convalescent Streptococcus equi subspecies equi M antibody titers, and presence of complications.. J Vet Intern Med 2019 Jan;33(1):275-279.
    pmc: PMC6335513pubmed: 30520521doi: 10.1111/jvim.15388google scholar: lookup
  24. Javed R, Taku AK, Gangil R, Sharma RK. Molecular characterization of virulence genes of Streptococcus equi subsp. equi and Streptococcus equi subsp. zooepidemicus in equines.. Vet World 2016 Aug;9(8):875-81.
    pmc: PMC5021838pubmed: 27651677doi: 10.14202/vetworld.2016.875-881google scholar: lookup
  25. Erol E, Locke SJ, Donahoe JK, Mackin MA, Carter CN. Beta-hemolytic Streptococcus spp. from horses: a retrospective study (2000-2010).. J Vet Diagn Invest 2012 Jan;24(1):142-7.
    pubmed: 22362945doi: 10.1177/1040638711434138google scholar: lookup
  26. Abdelsalam IO. Studies on Bacterial Pathogens Causing Respiratory Manifestations in Equine.. M.V.Sc. Degree (Microbiology:Bacteriology, Mycology, Immunology) Giza, Egypt: Faculty of Veterinary Medicine, Cairo University; 2019.
  27. Nasr SAE, Arafa AA. A novel foot bath for horse dwelling.. Int. J. Vet. Sci. 2019;8(2):106–112.
  28. Fonseca JD, Mavrides DE, Morgan AL, Na JG, Graham PA, McHugh TD. Antibiotic resistance in bacteria associated with equine respiratory disease in the United Kingdom.. Vet Rec 2020 Sep 5;187(5):189.
    pubmed: 32444507doi: 10.1136/vr.105842google scholar: lookup
  29. Yaghoobpour M, Fozouni L, Ghaemi P. New Solution for control of respiratory infection by Streptococcus equi isolated from horses in Northern Iran.. Bulgarian J. Vet. Med. 2021;2021:2–4.
  30. Seady M, Atwa S, Beskawy M, Elkhodary S, Eissa M, Ibrahim N. Clinical, molecular diagnosis and antimicrobial sensitivity of Streptococcus equi causing strangles.. Alex. J. Vet. Sci. 2018;56(2):78–83.
  31. Hassan AA, Khan IU, Abdulmawjood A, Lämmler C. Evaluation of PCR methods for rapid identification and differentiation of Streptococcus uberis and Streptococcus parauberis.. J Clin Microbiol 2001 Apr;39(4):1618-21.
    pmc: PMC87983pubmed: 11283100doi: 10.1128/jcm.39.4.1618-1621.2001google scholar: lookup
  32. Hassan AA, Akineden O, Lämmler C, Huber-Schlenstedt R. Molecular characterization of phenotypically CAMP-negative Streptococcus agalactiae isolated from bovine mastitis.. J Vet Med B Infect Dis Vet Public Health 2002 Jun;49(5):257-9.
    pubmed: 12121048doi: 10.1046/j.1439-0450.2002.00553.xgoogle scholar: lookup
  33. Estuningsih S, Soedarmanto I, Fink K, Lämmler C, Wibawan IW. Studies on Streptococcus agalactiae isolated from bovine mastitis in Indonesia.. J Vet Med B Infect Dis Vet Public Health 2002 May;49(4):185-7.
    pubmed: 12069271doi: 10.1046/j.1439-0450.2002.00482.xgoogle scholar: lookup
  34. Meiri-Bendek I, Lipkin E, Friedmann A, Leitner G, Saran A, Friedman S, Kashi Y. A PCR-based method for the detection of Streptococcus agalactiae in milk.. J Dairy Sci 2002 Jul;85(7):1717-23.
    pubmed: 12201522doi: 10.3168/jds.s0022-0302(02)74245-8google scholar: lookup
  35. Abdulmawjood A, Lämmler CH. Determination of intraspecies variations of the V2 region of the 16S rRNA gene of Streptococcus equi subsp. zooepidemicus.. Res Vet Sci 2000 Feb;68(1):33-9.
    pubmed: 10684756doi: 10.1053/rvsc.1999.0332google scholar: lookup
  36. Chanter N, Collin N, Holmes N, Binns M, Mumford J. Characterization of the Lancefield group C streptococcus 16S-23S RNA gene intergenic spacer and its potential for identification and sub-specific typing.. Epidemiol Infect 1997 Apr;118(2):125-35.
    pmc: PMC2808778pubmed: 9129589doi: 10.1017/s0950268896007285google scholar: lookup
  37. Hassan AA, Khan IU, Abdulmawjood A, Lämmler C. Inter- and intraspecies variations of the 16S-23S rDNA intergenic spacer region of various streptococcal species.. Syst Appl Microbiol 2003 Mar;26(1):97-103.
    pubmed: 12747416doi: 10.1078/072320203322337371google scholar: lookup
  38. Timoney JF, Artiushin SC. Detection of Streptococcus equi in equine nasal swabs and washes by DNA amplification.. Vet Rec 1997 Oct 25;141(17):446-7.
    pubmed: 9369003doi: 10.1136/vr.141.17.446google scholar: lookup
  39. Newton JR, Verheyen K, Talbot NC, Timoney JF, Wood JL, Lakhani KH, Chanter N. Control of strangles outbreaks by isolation of guttural pouch carriers identified using PCR and culture of Streptococcus equi.. Equine Vet J 2000 Nov;32(6):515-26.
    pubmed: 11093626doi: 10.2746/042516400777584721google scholar: lookup
  40. Alber J, El-Sayed A, Lämmler C, Hassan AA, Weiss R, Zschöck M. Multiplex polymerase chain reaction for identification and differentiation of Streptococcus equi subsp. zooepidemicus and Streptococcus equi subsp. equi.. J Vet Med B Infect Dis Vet Public Health 2004 Dec;51(10):455-8.
    pubmed: 15606870doi: 10.1111/j.1439-0450.2004.00799.xgoogle scholar: lookup

Citations

This article has been cited 6 times.
  1. Seeger MG, de Vargas APC, Vogel FSF, Cargnelutti JF. Streptococcus equi subsp. equi isolated from horses in Southern Brazil: molecular and phenotypic analyses. Braz J Microbiol 2025 Dec;56(4):2987-2996.
    doi: 10.1007/s42770-025-01783-xpubmed: 41032072google scholar: lookup
  2. Abdul-Latif SAK, Yousif AA. Molecular study of Streptococcus equi isolated from horses with strangles in Iraq. Open Vet J 2025 Feb;15(2):731-737.
    doi: 10.5455/OVJ.2025.v15.i2.22pubmed: 40201849google scholar: lookup
  3. Sabur A, Khan A, Borphukan B, Razzak A, Salimullah M, Khatun M. The Unique Capability of Endolysin to Tackle Antibiotic Resistance: Cracking the Barrier. J Xenobiot 2025 Jan 25;15(1).
    doi: 10.3390/jox15010019pubmed: 39997362google scholar: lookup
  4. Bekele D, Dessalegn B, Tadesse B, Abey SL. Streptococcus equi subspecies equi from strangles suspected equines: molecular detection, antibiogram profiles and risk factors. BMC Vet Res 2024 Aug 23;20(1):377.
    doi: 10.1186/s12917-024-04236-zpubmed: 39180060google scholar: lookup
  5. Soliman R, Yousef M, Gelil SA, Aboul-Ella H. Development of novel Streptococcus equi vaccines with an assessment of their immunizing potentials and protective efficacies. BMC Vet Res 2024 May 3;20(1):173.
    doi: 10.1186/s12917-024-04012-zpubmed: 38702665google scholar: lookup
  6. Zu H, Sun R, Li J, Guo X, Wang M, Guo W, Wang X. Development of a Real-Time Recombinase-Aided Amplification Method for the Rapid Detection of Streptococcus equi subsp. equi. Microorganisms 2024 Apr 11;12(4).
    doi: 10.3390/microorganisms12040777pubmed: 38674721google scholar: lookup
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