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Veterinary world2021; 14(7); 1808-1814; doi: 10.14202/vetworld.2021.1808-1814

Vancomycin resistant Streptococcus equi subsp. equi isolated from equines suffering from respiratory manifestation in Egypt.

Abstract: Upper respiratory tract infections are common in horses and can be caused by a variety of pathogens, mainly subsp, which are a significant equine pathogen causing major health issues as well as financial losses to the equine industry. This study aimed to determine the prevalence of bacteria in equines in Egypt, and characterize vancomycin-resistant subsp phenotypically and genotypically. Methods: subsp was isolated from internal nares of horses. All strains were confirmed by polymerase chain reaction-based detection of genus-specific , A and I genes. Antibiotic susceptibility was determined phenotypically using the disk diffusion method. Genotypic detection of antibiotic resistance genes was performed by analyzing as b-lactamase resistance (Z), tetracycline resistance (K), vancomycin resistance (A), and chloramphenicol resistance (A). Results: Eight streptococcal isolates were confirmed as . subsp. The genotypic characterization of antibiotic resistance showed resistance to A and K, with a frequency of 87.5% and 12.5%, respectively, while the frequency of sensitivity was 100% for gene and A gene. Conclusions: In this study, we assessed vancomycin-resistant subsp from equines suffering from respiratory manifestation in Egypt.
Copyright: © Arafa, et al.
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Publication Date: 2021-07-14 PubMed ID: 34475702PubMed Central: PMC8404119DOI: 10.14202/vetworld.2021.1808-1814Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research study explores the prevalence and properties of vancomycin-resistant Streptococcus equi subsp. equi, a bacterium that causes upper respiratory tract infections in horses, in Egypt.

Study Purpose and Methodology

  • The main goal of this research was to identify the prevalence of Streptococcus equi subsp. equi (S. equi) in horses in Egypt and to further analyze any vancomycin-resistant strains both phenotypically and genotypically.
  • S. equi was collected from the internal noses of horses for examination. The identity of these strains was then confirmed using polymerase chain reaction (PCR), a method of replicating DNA in a lab to create enough for analysis. Here, it was used to detect specific S. equi genes.
  • Antibiotic susceptibility was gauged phenotypically by the disk diffusion method, which involves placing a disk soaked in antibiotics on a culture of the bacteria to see how effectively it inhibits bacterial growth.
  • The exploration for antibiotic resistance genes was performed genotypically by seeking genes that facilitate resistance to b-lactamase (Z), tetracycline (K), vancomycin (A), and chloramphenicol (A).

Study Results

  • In the course of the investigation, eight isolates were identified as S. equi subsp. equi.
  • The genotypic characterization revealed resistance to tetracycline (K) with a frequency of 12.5% and vancomycin (A) with 87.5% frequency among the isolates.
  • The frequency of sensitivity was found to be 100% for the b-lactamase gene and chloramphenicol gene.

Study Conclusions

  • This research succeeded in assessing vancomycin-resistant S. equi from horses in Egypt suffering from respiratory conditions.
  • The high prevalence of vancomycin resistance is notable, particularly given the role of S. equi in causing significant health and financial impacts to the equine industry.

Cite This Article

APA
Arafa AA, Hedia RH, Ata NS, Ibrahim ES. (2021). Vancomycin resistant Streptococcus equi subsp. equi isolated from equines suffering from respiratory manifestation in Egypt. Vet World, 14(7), 1808-1814. https://doi.org/10.14202/vetworld.2021.1808-1814

Publication

Veterinary world
ISSN: 0972-8988
NlmUniqueID: 101504872
Country: India
Language: English
Volume: 14
Issue: 7
Pages: 1808-1814

Researcher Affiliations

Arafa, Amany A
  • Department of Microbiology and Immunology, National Research Centre, Dokki, Egypt.
Hedia, Riham H
  • Department of Microbiology and Immunology, National Research Centre, Dokki, Egypt.
Ata, Nagwa S
  • Department of Microbiology and Immunology, National Research Centre, Dokki, Egypt.
Ibrahim, Eman S
  • Department of Microbiology and Immunology, National Research Centre, Dokki, Egypt.

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Citations

This article has been cited 6 times.
  1. Seeger MG, de Vargas APC, Vogel FSF, Cargnelutti JF. Streptococcus equi subsp. equi isolated from horses in Southern Brazil: molecular and phenotypic analyses. Braz J Microbiol 2025 Dec;56(4):2987-2996.
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