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Reproduction in domestic animals = Zuchthygiene2021; 56(6); 848-856; doi: 10.1111/rda.13924

Variation among stallions in sperm quality after single layer centrifugation.

Abstract: Although single layer centrifugation (SLC) selects robust spermatozoa from stallion semen, the effect of individual variation has not been studied in detail. The objective of this study was to determine the variation among stallions in the effects of SLC on sperm quality during cooled storage for up to 48 hr. Semen samples from seven stallions (18 ejaculates) were split, with one portion being used for SLC and the other serving as a control (CON). Sperm quality (kinematics, reactive oxygen species (ROS) production, membrane integrity (MI) and chromatin integrity) were analysed at 0, 24 and 48 hr using computer-assisted sperm analysis and flow cytometry. Sperm quality was better in SLC than in CON at all timepoints, especially chromatin integrity and MI (p < .0001 for both), and some categories of ROS production (e.g. proportion of live hydrogen peroxide negative spermatozoa, p < .0001), but the degree of improvement varied among stallions and type of ROS (p < .05-p < .0001). Total and progressive motility were also better in SLC samples than in CON at 24 and 48 hr (p < .0001), although the effect on sperm kinematics varied. The interaction of treatment, time and stallion was not significant. In conclusion, sperm quality was better in SLC samples than in CON, although there was considerable individual variation among stallions. The improvement in sperm quality, particularly in chromatin integrity, was clearly beneficial, and therefore the use of this technique would be warranted for all stallion semen samples.
© 2021 The Authors. Reproduction in Domestic Animals published by Wiley-VCH GmbH.
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Publication Date: 2021-03-19 PubMed ID: 33706415DOI: 10.1111/rda.13924Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study explores the variability in sperm quality among stallions after undergoing a specific procedure called single-layer centrifugation (SLC). Results revealed that SLC led to improved sperm quality across samples, with notable benefits in chromatin integrity, though the degree of improvement varied between individual stallions.

Objective of the Study

  • This research aimed to investigate the effects, and the level of individual variation, of single-layer centrifugation (SLC) on stallion sperm quality during cooled storage for up to 48 hours.

Methodology

  • Semen samples were obtained from seven stallions, totalling 18 ejaculates.
  • Each sample was split into two, with one part being processed using SLC, and the other serving as a control sample.
  • The researchers examined sperm quality, specifically kinematics, reactive oxygen species (ROS) production, membrane integrity (MI), and chromatin integrity, across different time intervals (0, 24, and 48 hours).
  • Data collection and analysis were done using computer-assisted sperm analysis and flow cytometry.

Results

  • The study found that sperm quality was generally better in samples treated with SLC compared to control samples at all time points.
  • Significant improvements were seen in chromatin integrity and membrane integrity.
  • SLC also effectively reduced some types of ROS production. This is crucial as ROS can be harmful to sperm cells, causing both structural damage and potential DNA damage.
  • SLC-treated samples displayed improved total and progressive motility compared to control samples.
  • However, the degree of improvement in these measures varied among stallions, indicating individual variation in response to SLC.
  • The interaction between the treatment, time, and individual stallion did not show significance, affirming the dominant role of the treatment itself in the results.

Conclusions

  • The study concluded that SLC significantly improves sperm quality, especially chromatin integrity, across stallion samples, even though the level of improvement can vary between individuals.
  • Given the above results, the researchers suggested that SLC should be utilized for all stallion semen samples to enhance their quality.

Cite This Article

APA
Al-Kass Z, Brown A, Johannisson A, Ntallaris T, Morrell JM. (2021). Variation among stallions in sperm quality after single layer centrifugation. Reprod Domest Anim, 56(6), 848-856. https://doi.org/10.1111/rda.13924

Publication

Reproduction in domestic animals = Zuchthygiene
ISSN: 1439-0531
NlmUniqueID: 9015668
Country: Germany
Language: English
Volume: 56
Issue: 6
Pages: 848-856

Researcher Affiliations

Al-Kass, Ziyad
  • Clinical Sciences, Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden.
  • Department of Surgery and Theriogenology, College of Veterinary Medicine, University of Mosul, Mosul, Iraq.
Brown, Alexander
  • Clinical Sciences, Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden.
Johannisson, Anders
  • Clinical Sciences, Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden.
Ntallaris, Theodoros
  • Clinical Sciences, Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden.
Morrell, Jane M
  • Clinical Sciences, Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden.

MeSH Terms

  • Animals
  • Cell Membrane
  • Centrifugation / methods
  • Centrifugation / veterinary
  • DNA Fragmentation
  • Horses
  • Male
  • Reactive Oxygen Species / analysis
  • Semen Analysis / veterinary
  • Semen Preservation / veterinary
  • Sperm Motility
  • Spermatozoa / physiology

Grant Funding

  • NA / Sveriges Lantbruksuniversitet
  • NA / Ministry of Higher Education and Scientific Research, Iraq.
  • vacation scholarship / Society for Reproduction and Fertility

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This article includes 25 references
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Citations

This article has been cited 3 times.
  1. Gutiérrez-Cepeda L, Crespo F, Blazquez JC, Serres C. Optimization of the Equine-Sperm Freeze Test in Purebred Spanish Horses by Incorporating Colloidal Centrifugation. Animals (Basel) 2023 Jan 22;13(3).
    doi: 10.3390/ani13030382pubmed: 36766271google scholar: lookup
  2. Al-Kass Z, Morrell JM, Ntallaris T. Effect of Centrifugation of Stallion Semen Through a Low Density Colloid Prior to Freezing on Sperm Cryosurvival. Animals (Basel) 2025 Jun 25;15(13).
    doi: 10.3390/ani15131881pubmed: 40646780google scholar: lookup
  3. Khan IU, Khairullah AR, Khan AY, Rehman AU, Mustofa I. Strategic approaches to improve equine breeding and stud farm outcomes. Vet World 2025 Feb;18(2):311-328.
    doi: 10.14202/vetworld.2025.311-328pubmed: 40182817google scholar: lookup
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