Acta parasitologica
Publisher:
Witold Stefanski Institute Of Parasitology. Cham : Springer International Publishing (2019)
Frequency: Quarterly
Country: Switzerland
Language: English
Author(s):
Instytut Parazytologii im. Witolda Stefańskiego.
Start Year:1992 -
ISSN:
1230-2821 (Print)
1896-1851 (Electronic)
1230-2821 (Linking)
1896-1851 (Electronic)
1230-2821 (Linking)
Impact Factor
1.5
2022
| NLM ID: | 9301947 |
| (DNLM): | SR0075517(s) |
| (OCoLC): | 26496009 |
| Coden: | ACTPEO |
| LCCN: | 95656541 |
| Classification: | W1 AC906J |
Seroprevalence of Toxoplasma gondii in Equids: A Global Systematic Review and Meta-analysis. Toxoplasmosis is a zoonotic infectious disease caused by T. gondii, a widely distributed obligate intracellular parasite. It has received widespread attention because it can infect a large number of domestic and wild animals, thereby posing a threat to public health and the economy on a global scale. Among them, equine animals are critical intermediate hosts. However, studies on T. gondii infection in equine animals are currently not systematic on a global scale. For this purpose, we conducted a systematic review and meta-analysis. Methods: This study searched for relevant studies in six signi...
Prevalence of Theileria Equi and Babesia Caballi and Associated Hemato-Biochemical Changes in Racing Horses in Erbil Province, Iraq. This study aimed to determine the prevalence of Theileria equi (T. equi) and Babesia caballi (B. caballi) in racing horses in Erbil Province, Iraq, and to assess the associated hemato-biochemical changes in infected animals. Methods: A total of 196 horses, including 155 clinically healthy and 41 clinically suspected infected animals, were examined using Giemsa-stained blood films and PCR to determine the prevalence rates. Additionally, the hemato-biochemical parameters of infected horses were compared to a non-infected control group (n = 10). Results: The prevalence of T. equi and B. cabal...
Equine Piroplasmosis in Asymptomatic Horses of Western Iran: Comparison of Microscopic Examination and Multiplex PCR. Piroplasmosis is responsible for anemia, fever, loss of physical activity and even death in equines. In epidemiological studies, accurate diagnostic tests are essential for detecting asymptomatic carriers. This study aimed to investigate the prevalence of infection in asymptomatic horses from Lorestan province, western Iran by developing a multiplex PCR. Results: Blood samples were examined by microscopy and multiplex PCR targeting the SSU rRNA gene of Theileria equi and Babesia caballi. Out of the total of 165 horses, 19 (11.51%) and 31 (18.78%) cases were positive for piroplasms by microscop...
Molecular Detection of Animal Trypanosomes in Different Animal Species in the Visayas Region of the Philippines. Animal trypanosomosis is one of the most important parasitic diseases significantly affecting the Philippine economy. It is considered by the government to be the second most important disease of livestock after fasciolosis. A PCR-based molecular survey for trypanosomes in different animals in Bohol, Philippines, was performed to assess the prevalence of trypanosomosis in the area during the rainy and dry season. Methods: A total of 269 blood samples were collected in two batches in rainy and dry season from different animal species in Ubay Stock Farm in Ubay, Bohol, the Philippines, including...
Gastrointestinal Helminths in Horses in Serbia and Various Factors Affecting the Prevalence. Data on gastrointestinal infections in horses in Southeastern Europe are limited; thus, this study aimed to add to the existing knowledge on this topic by reporting on the prevalence of intestinal parasites of horses in the territory of the Republic of Serbia. Methods: In the period from April 2017 to December 2018, parasitological examination of 548 samples from horses of different breed, age and sex from four regions and 18 districts of the country was performed. Coprological diagnostic was done by using qualitative methods without concentration and qualitative methods with concentration of ...
Gastrointestinal Parasitism in Przewalski Horses (Equus ferus przewalskii). Przewalski's horse (Equus ferus przewalskii) is a subspecies of Equus ferus classified as endangered by the International Union for Conservation of Nature (IUCN), whose gastrointestinal (GI) parasitism has been studied in different populations, in situ and ex situ, during the last decades. We aimed to summarize the information available on the subject. Methods: In this review, we collected information from distinct published literature, interpreted it, compared it and related the Przewalski's horse biology and ecology with different findings. Results: Many similarities can be found between the...
Hematological and Biochemical Changes in Naturally Occurring Equine Piroplasmosis in Donkeys (Equus asinus) of Northwest of Iran. Equine piroplasmosis is a tick-borne protozoal disease affecting horses, mules, donkeys and zebras and can lead to severe economic losses in the equine industry. There is limited information on clinical pathologic changes of equine piroplasmosis in donkeys. This study aims to investigate the impacts of babesiosis on various hematological and biochemical properties in naturally infected donkeys with Theileria equi and Babesia caballi. Methods: A total of 200 apparently healthy donkeys were randomly examined for the presence of equine piroplasmosis in blood samples. Of 200 samples, 11(5.5%) anim...
First Report Regarding the Simultaneous Molecular Detection of Anaplasma marginale and Theileria annulata in Equine Blood Samples Collected from Southern Punjab in Pakistan. The present study was designed to check the molecular detection of Anaplasma marginale and Theileria annulata in blood samples of horses and donkeys collected from Dera Ghazi Khan District in Punjab and to document their phylogenetic origin and their association with studied epidemiological factors (sex and age) and complete blood count parameters, if any. Results: A total of 195 blood samples were collected from apparently healthy horses (N = 141) and donkeys (N = 54). A. marginale DNA was detected by PCR in 4.9% (7/141) horse and in 9.2% (5/54) of donkey blood samples. Prevalence of ...
Toxoplasma gondii Seroprevalence in Horses from Ukraine: an Investigation Using Two Serological Methods. Horses are recognized as important hosts for the zoonotic parasite Toxoplasma gondii, mainly because meat of infected horses can be a source of human T. gondii infections. However, the baseline knowledge on equine T. gondii infections is limited and lacking from many countries. This seroepidemiological study was set in Ukraine, a country where little is known about T. gondii prevalence in any host species. Methods: We investigated 78 serum samples from horses, collected from Kyiv and Lviv regions of Ukraine, using a commercial enzyme-linked immunosorbent assay (ELISA) and a commercial modified...
Evaluation and correlation of oxidative stress and haemato-biochemical observations in horses with natural patent and latent trypanosomosis in Punjab state of India. The haemato-biochemical indices and oxidative stress markers in horses naturally infected with Trypanosoma evansi were evaluated by analyzing the level of these parameters between T. evansi infected (microscopically positive patent group and PCR positive latent group) and infection free horses. To compare the hemato-biochemical indices and oxidative stress indicators, horses were divided into three categories based on diagnostic test employed and positive results obtained. These included Romanowsky stained slide positive group (Group I; n = 6), PCR positive group (group II; n = 28) and negativ...
Abundance and species composition of Culicoides spp. biting midges near cattle and horse in South-Eastern Poland. The aim of the study was to estimate and compare the distribution of Culicoides biting midges species at farms with different main hosts - cattle and horse. Culicoides spp. are known vectors of arboviruses including African horse sickness virus (AHSV), bluetongue virus (BTV) and Schmallenberg virus (SBV). The latter two have been already reported in Polish ruminants recently, while AHSV remains absent, however the risk of its emergence has been increasing in the recent years. In order to establish the activity of potential AHSV vector at vicinity of horses, an OVI midge trap has been placed at...
Seroprevalence and risk factors of Neospora spp. and Toxoplasma gondii infections among horses and donkeys in Nigeria, West Africa. Neospora spp. and Toxoplasma gondii are considered to be a globally distributed parasites affecting wide range of warm-blooded animals. Neosporosis has caused clinical illness in horses and consumption of horse meat has been epidemiologically linked to clinical toxoplasmosis in humans. This study was conducted to determine Neospora spp. and T. gondii antibodies and risk factors of infection in horses and donkeys from three states of Nigeria. A total of 144 samples were collected from clinically healthy animals (120 horses and 24 donkeys). The sera were tested for antibodies to Neospora spp. an...
Serosurvey of anti-Toxoplasma gondii antibodies in sport horses from Paraiba state, Northeastern Brazil. Although the infection by Toxoplasma gondii in horses is usually asymptomatic, it may be characterized by the presence of tissue cysts and maintenance of detectable antibody titers. The aim of this study was to detect anti-T.gondii antibodies and to determine risk factors associated in sport horses from Paraíba State, Northeastern Brazil. A total of 138 apparently healthy sport horses of different age and gender were sampled. Anti-Toxoplasma gondii antibodies were detected in 16/138 (11.6%; 95% CI: 6.77-18.14%) horses by indirect immunofluorescence antibody assay (IFA). A significant associat...
Prevalence and molecular characterization of Theileria equi and Babesia caballi in jereed horses in Erzurum, Turkey. Equine piroplasmosis (EP) is a hemoprotozoan tick-borne disease with worldwide distribution that is caused by Theileria equi and Babesia caballi. There are studies reporting the presence of equine piroplasmosis in Turkey but the situation in Erzurum is unknown. The aim of the current study was to determine the situation of equine piroplasmosis in jeered horses in Erzurum. Between April and August 2015, a total of 125 Arabian horse were examined and blood samples were collected. At the time of sampling, animals were also examined for tick infestations and clinical signs. Besides microscopic exa...
Molecular detection and prevalence of Theileria equi and Babesia caballi in horses of central Balkan. Equine piroplasmosis is significant tick-borne disease with wide distribution. The prevalence of equine piroplasmosis in Serbia, Montenegro and Bosnia and Herzegovina is unknown. In aim to obtain a first insight into the prevalence we performed molecular epidemiological study which included 142 horses, on seven locations in these three countries. We first performed PCR for the detection of a 450bp long section of the 18S rRNA of piroplasma-specific region. For all positive samples we have done multiplex PCR for the species detection. Species determination was further confirmed by sequencing PC...
Risk factors of occurrence of Toxoplasma gondii among horses in the state of Alagoas, Brazil. The aim of the present study was to investigate the occurrence of Toxoplasma gondii among horses and its associated risk factors in Alagoas, Brazil. In total, 440 samples from 36 properties in 23 districts of the state of Alagoas were studied, covering the Leste, Agreste and Sertão mesoregions. Risk factors were evaluated through the application of an investigative questionnaire that focused on the productive, reproductive and sanitary management of herds. T. gondii infection were assayed using the immunofluorescence assay (IFA) with a cutoff point of 64; 14.4% (95% CI: 11.0%-17.8%) of - hors...
Production and preliminary evaluation of Trypanosoma evansi HSP70 for antibody detection in Equids. The present immuno-diagnostic method using soluble antigens from whole cell lysate antigen for trypanosomosis have certain inherent problems like lack of standardized and reproducible antigens, as well as ethical issues due to in vivo production, that could be alleviated by in vitro production. In the present study we have identified heat shock protein 70 (HSP70) from T. evansi proteome. The nucleotide sequence of T. evansi HSP70 was 2116 bp, which encodes 690 amino acid residues. The phylogenetic analysis of T. evansi HSP70 showed that T. evansi occurred within Trypanosoma clade and is most c...
Molecular evidence of Theileria equi infection in Hyalomma anatolicum ticks infested on sero-positive Indian horses. A sizeable Indian equine population is considered to be pre-immune carrier of Theileria equi infection. In this study we confirmed the presence of T. equi specific DNA in Hyalomma anatolicum ticks which were infested on sero-positive horses. Fifty two Indigenous horses were randomly selected from endemic areas and their blood and tick samples were collected. Tick salivary glands and blood samples were processed for separation of DNA and serum, respectively. Serum samples were analyzed by EMA-2ELISA and nine horses were found positive for T. equi specific antibodies. Species-specific primers we...
Detection of Theileria equi in spleen and blood of asymptomatic piroplasm carrier horses. This study aimed to determine whether asymptomatic horses naturally infected with Theileria equi retain infected erythrocytes in the spleen and whether the presence of the hemoparasite in this organ is associated with parasitemia. We collected samples from 25 adult horses without clinical signs of any disease. From each animal, we collected whole blood samples from the jugular vein and a splenic puncture blood sample. All samples were submited to blood cell counts and detection of Theileria or Babesia. DNA extraction and PCR were performed in all samples for identification of piroplasm infecti...