Forensic science international.
Publisher:
Elsevier Sequoia.. Limerick : Elsevier Science Ireland
Frequency: Twenty one no. a year
Country: Ireland
Language: English
Start Year:1978 -
ISSN:
0379-0738 (Print)
1872-6283 (Electronic)
0379-0738 (Linking)
1872-6283 (Electronic)
0379-0738 (Linking)
Impact Factor
2.2
2022
| NLM ID: | 7902034 |
| (DNLM): | F10650000(s) |
| (OCoLC): | 04590488 |
| Coden: | FSINDR |
| Classification: | W1 FO615I |
Detection using chamber digital PCR with a DNA extraction-free method for gene-doping control. Gene doping, which entails the administration of transgenes, poses a serious threat to the integrity of equine sports and also raises both ethical and regulatory concerns. Current methods used for the detection of such doping often necessitate the extraction of DNA from plasma, which can be time-consuming and labour-intensive. To overcome this limitation, we developed a direct chamber digital PCR (cdPCR) method that enables transgene detection in equine plasma without the need for DNA purification. Using the equine erythropoietin (EPO) transgene as a model, we validated the assay by analysing ...
Morphological differentiation of bovine and equine hair for species’ identification in forensic veterinary investigations. In this paper a morphological differentiation tool of bovine and equine hair for their identification in forensic investigations is provided. 30 animals were used, fifteen adult bovines of the "Siboney de Cuba" breed and fifteen adult equines of the "Criolla" breed. These species are the most bred in Cuba and, at the same time, the most exposed to poaching and theft crimes. Hair from the cows was sampled from the dorsal region, the lateral region and the ventral region; on the other hand, the horses' hair was sampled from the dorsal region of the body, of the mane and of the tail. The sampled ...
Experimental and theoretical studies into the release of blood droplets from weapon tips. The formation and release under gravity of blood droplets from simulated weapon tips has been investigated experimentally and the results analysed and interpreted using established theoretical models for detached pendent drops. Droplets were produced from a series of conical nozzles, manufactured with a range of cone angles and including a set of un-bored conical tips, under conditions where the tip was either non-wetted or pre-wetted with blood. For the former, radius-limited case, detached droplet volumes were found to agree well with the predictions of both the pendent drop weight and drop ...
Simulating forensic casework scenarios in experimental studies: The generation of footwear marks in blood. A study was designed to investigate the effects of external variables, including blood type, flooring surface, footwear tread depth and blood dryness, on the appearance of blood-based footwear marks, with particular reference to simulating a specific casework scenario. Results showed that footwear marks left in human blood tended to be of greater quality than those in equine blood, highlighting a potential issue in applying data generated with equine blood to human bloodstains in casework. Footwear tread effects were also dependent on blood type, but the type of flooring surface did not affect...
Multiple isotope forensics of nitrate in a wild horse poisoning incident. Multiple stable isotope analysis can be a powerful technique in forensic sciences. Oxygen and nitrogen isotopes were used to determine the source of nitrate that was responsible for the poisoning deaths of 71 wild horses in the Nevada desert. The nitrate was present in a water-filled hole known as 'the Main Lake depression.' Nitrate from the Main Lake depression had delta(18)O and delta(15)N values that were very positive (+32 per thousand, +37 per thousand), and Delta(17)O values of approximately +2 per thousand. The isotopic data suggested that the most probable source of the nitrate was nit...
Detection and quantification of low levels of benzoylecgonine in equine urine. Cocaine (COC) is a highly addictive plant alkaloid expressing strong psychostimulatory effect. It has no medical use in equine veterinary practice. The contamination of the environment with cocaine such as its presence on the US paper currency has been reported few times. There are anecdotal reports of low benzoylecgonine (BE) concentrations (usually much less than 100 ng/mL) being found in urine of race horses. In order to protect horsemen against harsh penalties associated with the presence of trace amounts of BE in horse urine as a result of environmental contamination, in February 2005 the...
Evaluation of high performance liquid chromatography (HPLC), enzyme linked immunosorbent assay (ELISA) and particle concentration fluorescence immunoassay (PCFIA) methods for the screening, quantitation and pharmacokinetic study of furosemide in horses. Equine plasma and urine samples were analyzed by using a high-performance liquid chromatography (HPLC), enzyme-linked immunosorbent assay (ELISA) and particle concentration fluorescence assay (PCFIA). Although ELISA and PCFIA were rapid, simple and sensitive for the screening of furosemide, they did not give reproducible quantitative results. The HPLC method, which required relatively longer analysis time, provided simple and reproducible quantitative analysis of furosemide in plasma and urine. The performance of the three methods was compared for the quantitation of furosemide in plasma obtai...
The isoelectric focusing of keratins in hair followed by silver staining. An isoelectric focusing method followed by silver staining has been developed for the study of keratins which is as effective as two-dimensional electrophoresis and fluorography for hair species identification. Hair from dogs, rabbits, horses, cows, guinea-pigs, donkeys, sheep and cats were successfully identified. Narrow pH ranges were used to observe heterogeneity in human hair. Although this heterogeneity may be affected by environmental conditions, it may be of use in criminalistics.