Veterinary parasitology.
Periodical
Parasitology
Veterinary Medicine
Parasitic Diseases
Animal
Publisher:
Elsevier Scientific Pub. Co.. Amsterdam : Elsevier
Frequency: Monthly, 2019-
Country: Netherlands
Language: English
Start Year:1975 -
ISSN:
0304-4017 (Print)
1873-2550 (Electronic)
0304-4017 (Linking)
1873-2550 (Electronic)
0304-4017 (Linking)
Impact Factor
2.6
2022
| NLM ID: | 7602745 |
| (DNLM): | V05960000(s) |
| (OCoLC): | 01939255 |
| Coden: | VPARDI |
| Classification: | W1 VE933U |
Use of schizont and piroplasm antigens of Babesia equi in the indirect fluorescent antibody and complement fixation tests.
Veterinary parasitology
May 1, 1983
Volume 12, Issue 2 135-144 doi: 10.1016/0304-4017(83)90002-x
Rehbein G, Heidrich-Joswig S.Eight ponies infected with Babesia equi were investigated for their serological response to B. equi schizont and piroplasm antigen with the indirect fluorescent antibody test (IFAT) and complement fixation test (CFT). Piroplasm antigen was prepared from an infected splenectomized pony, while schizont antigen was produced from cultured lymphoid cells which contained B. equi macroschizonts. The IFAT detected a rise in antibody titres to schizont antigen as well as to piroplasm antigen, but differences were obtained in the duration of antibody detection. Significant antibody titres to piroplasm a... The activity of closantel as an equine antiparasitic agent.
Veterinary parasitology
February 1, 1983
Volume 12, Issue 1 71-77 doi: 10.1016/0304-4017(83)90090-0
Guerrero J, Michael BF, Rohovsky MW, Campbell BP.Eighteen pony foals were experimentally infected with 500 third stage larvae of Strongylus vulgaris at 2 weeks, and at 2, 4, 6 and 8 months after birth. For the duration of the study, all foals were kept in the same pasture with their mothers to allow natural infection with other parasites by exposure to a contaminated environment. Twelve of the foals were utilized in groups of 3 and treated orally five times at two month intervals starting at one month of age with closantel at doses of 5, 10, 20 or 40 mg kg-1. Ten months after birth the foals were necropsied to determine the parasitic burdens... Critical tests in equids with fenbendazole alone or combined with piperazine: particular reference to activity on benzimidazole-resistant small strongyles.
Veterinary parasitology
February 1, 1983
Volume 12, Issue 1 91-98 doi: 10.1016/0304-4017(83)90092-4
Lyons ET, Tolliver SC, Drudge JH.Seven critical tests in equids were conducted with single doses of fenbendazole (5 mg kg-1) alone (Panacur--American Hoechst, Somerville, NJ); (2 tests with paste and 1 with suspension formulation) or in combination with piperazine (American Hoechst); (40 mg base kg-1); (4 tests with paste formulation). The main purpose of the tests was evaluation of activity against benzimidazole-resistant small strongyles (Cyathostomum catinatum, Cyathostomum coronatum, Cylicocyclus nassatus, Cylicostephanus goldi, and Cylicostephanus longibursatus). Natural infections of 2 populations of benzimidazole-resis... The longevity of hydatid cysts in horses.
Veterinary parasitology
November 1, 1982
Volume 11, Issue 2-3 149-154 doi: 10.1016/0304-4017(82)90037-1
Ronéus O, Christensson D, Nilsson NG.No abstract available The prevalence of Gasterophilus intestinalis in horses in northern England and Wales.
Veterinary parasitology
November 1, 1982
Volume 11, Issue 2-3 215-222 doi: 10.1016/0304-4017(82)90044-9
Edwards GT.The stomachs of 448 horses from northern England and Wales were examined for Gasterophilus larvae, and 237 (52.7%) were found to be infected with G. intestinalis. Larvae were present in stomachs examined during each month of the year except August. Second instar larvae occurred from September through February and third instars were present from November through July. Adult fly activity began in August as indicated by the presence of eggs on horses. The life-cycle of G. intestinalis in northern England and Wales is outlined from the data presented. The mean instar burdens were 15.7 second and 3... World Association for the Advancement of Veterinary Parasitology (W.A.A.V.P.) methods for the detection of anthelmintic resistance in nematodes of veterinary importance.
Veterinary parasitology
September 1, 1992
Volume 44, Issue 1-2 35-44 doi: 10.1016/0304-4017(92)90141-u
Coles GC, Bauer C, Borgsteede FH, Geerts S, Klei TR, Taylor MA, Waller PJ.Methods have been described to assist in the detection of anthelmintic resistance in strongylid nematodes of ruminants, horses and pigs. Two tests are recommended, an in vivo test, the faecal egg count reduction test for use in infected animals, and an in vitro test, the egg hatch test for detection of benzimidazole resistance in nematodes that hatch shortly after embryonation. Anaerobic storage for submission of faecal samples from the field for use in the in vitro test is of value and the procedure is described. The tests should enable comparable data to be obtained in surveys in all parts o... Read More
401
An inconvenient truth: global worming and anthelmintic resistance.
Veterinary parasitology
November 20, 2011
Volume 186, Issue 1-2 70-78 doi: 10.1016/j.vetpar.2011.11.048
Kaplan RM, Vidyashankar AN.Over the past 10-15 years, we have witnessed a rapid increase in both the prevalence and magnitude of anthelmintic resistance, and this increase appears to be a worldwide phenomenon. Reports of anthelmintic resistance to multiple drugs in individual parasite species, and in multiple parasite species across virtually all livestock hosts, are increasingly common. In addition, since the introduction of ivermectin in 1981, no novel anthelmintic classes were developed and introduced for use in livestock until recently with the launch of monepantel in New Zealand. Thus, livestock producers are often... Read More
292
The detection of anthelmintic resistance in nematodes of veterinary importance.
Veterinary parasitology
January 19, 2006
Volume 136, Issue 3-4 167-185 doi: 10.1016/j.vetpar.2005.11.019
Coles GC, Jackson F, Pomroy WE, Prichard RK, von Samson-Himmelstjerna G, Silvestre A, Taylor MA, Vercruysse J.Before revised World Association for the Advancement of Veterinary Parasitology (WAAVP) guidelines on the detection of anthelmintic resistance can be produced, validation of modified and new methods is required in laboratories in different parts of the world. There is a great need for improved methods of detection of anthelmintic resistance particularly for the detection of macrocyclic lactone resistance and for the detection of resistant nematodes in cattle. Therefore, revised and new methods are provided here for the detection of anthelmintic resistance in nematodes of ruminants, horses and ... Read More
270
Babesia–a historical overview.
Veterinary parasitology
February 28, 2006
Volume 138, Issue 1-2 3-10 doi: 10.1016/j.vetpar.2006.01.035
Uilenberg G.The history of the genus Babesia is briefly outlined. The classical differences with the main other genus of non-pigment-forming hemoparasites, Theileria, are the absence of extra-erythrocytic multiplication (schizogony) in Babesia and the cycle in the vector tick, which includes transovarial transmission in Babesia but only transstadial transmission in Theileria. Also, the multiplication in the red cell of Babesia, by budding, most often results in two daughter cells (merozoites), while that of Theileria gives four merozoites, often as a Maltese cross. In particular this means that what is st... Read More
189
The natural history of Anaplasma phagocytophilum.
Veterinary parasitology
September 20, 2009
Volume 167, Issue 2-4 108-122 doi: 10.1016/j.vetpar.2009.09.013
Woldehiwet Z.Anaplasma phagocytophilum is the recently designated name replacing three species of granulocytic bacteria, Ehrlichia phagocytophila, Ehrlichia equi and the agent of human granulocytic ehrlichiosis, after the recent reorganization of the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales. Tick-borne fever (TBF), which is caused by the prototype of A. phagocytophilum, was first described in 1932 in Scotland. A similar disease caused by a related granulocytic agent was first described in horses in the USA in 1969; this was followed by the description of two distinct granulocy... Read More
140
Nested polymerase chain reaction for detection of Ehrlichia equi genomic DNA in horses and ticks (Ixodes pacificus).
Veterinary parasitology
June 1, 1996
Volume 63, Issue 3-4 319-329 doi: 10.1016/0304-4017(95)00904-3
Barlough JE, Madigan JE, DeRock E, Bigornia L.A nested polymerase chain reaction for detecting Ehrlichia equi in horses and ticks (Ixodes pacificus) was developed. A major second-round PCR product of 928 bp could be readily visualized in ethidium bromide-stained agarose minigels. An internal probe was used to verify the identity of the amplified product by non-radioactive (digoxigenin-based) Southern blotting; additional confirmation was provided by DNA sequence analysis. A dilution study testing the sensitivity of the PCR indicated that DNA derived from 3 infected neutrophils was sufficient to generate a PCR signal. The specificity of t... Read More
91
Pathogenicity of cyathostome infection.
Veterinary parasitology
September 15, 1999
Volume 85, Issue 2-3 113-225 doi: 10.1016/s0304-4017(99)00092-8
Love S, Murphy D, Mellor D.Cyathostomes are now the principle parasitic pathogen of the horse: a remarkable transformation during the last 25 years from virtual obscurity to focus of attention in equine parasitology. This rise to prominence coincides with the marked decrease in prevalence of large strongyle infections as a result of widespread use of modern anthelmintic compounds. On the basis that strongyle-associated diseases continue to commonly occur in the absence of these large strongyle species, clinical attention has turned to the pathogenicity of cyathostomes. Although many horses harbour burdens of tens of tho... Read More
82
Molecular studies on Babesia, Theileria and Hepatozoon in southern Europe. Part I. Epizootiological aspects.
Veterinary parasitology
April 30, 2003
Volume 113, Issue 3-4 189-201 doi: 10.1016/s0304-4017(03)00078-5
Criado-Fornelio A, Martinez-Marcos A, Buling-Saraña A, Barba-Carretero JC.Molecular epizootiology of piroplasmids (Babesia spp., Theileria spp.) and Hepatozoon canis was studied in mammals from southern Europe (mainly from Spain, but also from Portugal and France). Partial amplification and sequencing of the 18s rRNA gene was used for molecular diagnosis. In some particular cases (B. ovis and B. bovis) the complete 18s rRNA gene was sequenced. Blood samples were taken from domestic animals showing clinical symptoms: 10 dogs, 10 horses, 10 cows, 9 sheep and 1 goat. In addition, DNA samples were isolated from blood of 12 healthy dogs and from spleen of 10 wild red fox... Read More
79
Epidemiological study of the cystic echinococcosis in Morocco.
Veterinary parasitology
February 10, 2006
Volume 137, Issue 1-2 83-93 doi: 10.1016/j.vetpar.2006.01.003
Azlaf R, Dakkak A.The objectives of this epidemiological study on cystic echinococcosis (CE) in Morocco (2001-2004) were to update the prevalence of CE in different animal species living in the most important areas of the country and to collect protoscoleces and germinal layers for genetic research purposes. The post mortem inspection concerned 2948 sheep, 2337 goats, 618 cattle, 482 camels and 455 equines (325 horses, 60 mules and 70 donkeys) in five different regions: the Rif (Mediterranean coast and high mountains of the Rif), the Loukkos (Atlantic northwest plain), the center (Rabat and Casablanca regions),... Read More
75
Anthelmintic resistance in important parasites of horses: does it really matter?
Veterinary parasitology
January 15, 2014
Volume 201, Issue 1-2 1-8 doi: 10.1016/j.vetpar.2014.01.004
Peregrine AS, Molento MB, Kaplan RM, Nielsen MK.Parascaris equorum and cyathostomins are currently considered the most important parasites of horses and have traditionally been controlled with anthelmintics belonging to three drug classes: benzimidazoles, the tetrahydropyrimidine pyrantel, and macrocyclic lactones. Unfortunately, resistance to benzimidazoles, and to a lesser extent pyrantel, is widespread in cyathostomins around the world. Furthermore, resistance to macrocyclic lactones appears to be in the early stages of development in cyathostomins in multiple locations. In contrast, P. equorum populations have remained susceptible to th... Read More
67
Illustrated identification keys to strongylid parasites (Strongylidae: Nematoda) of horses, zebras and asses (Equidae).
Veterinary parasitology
May 21, 2008
Volume 156, Issue 1-2 4-161 doi: 10.1016/j.vetpar.2008.04.026
Lichtenfels JR, Kharchenko VA, Dvojnos GM.The Equidae (the horse, Equus caballus, the ass, Equus asinus, zebras and their hybrids) are hosts to a great variety of nematode parasites, some of which can cause significant morbidity or mortality if individual hosts are untreated. Worldwide the nematode parasites of horses belong to 7 suborders, 12 families, 29 genera and 83 species. The great majority (19 of 29 genera and 64 of 83 species) are members of the family Strongylidae, which includes the most common and pathogenic nematode parasites of horses. Only the Strongylidae are included in this treatise. The Strongylidae (common name str... Read More
62
World Association for the Advancement of Veterinary Parasitology (W.A.A.V.P.) guideline for diagnosing anthelmintic resistance using the faecal egg count reduction test in ruminants, horses and swine.
Veterinary parasitology
April 23, 2023
Volume 318 109936 doi: 10.1016/j.vetpar.2023.109936
Kaplan RM, Denwood MJ, Nielsen MK, Thamsborg SM, Torgerson PR, Gilleard JS, Dobson RJ, Vercruysse J, Levecke B.The faecal egg count reduction test (FECRT) remains the method of choice for establishing the efficacy of anthelmintic compounds in the field, including the diagnosis of anthelmintic resistance. We present a guideline for improving the standardization and performance of the FECRT that has four sections. In the first section, we address the major issues relevant to experimental design, choice of faecal egg count (FEC) method, statistical analysis, and interpretation of the FECRT results. In the second section, we make a series of general recommendations that are applicable across all animals ad... Read More
61
Seasonal dynamics of ticks (Acari: Ixodidae) on horses in the state of São Paulo, Brazil.
Veterinary parasitology
March 7, 2002
Volume 105, Issue 1 65-77 doi: 10.1016/s0304-4017(01)00649-5
Labruna MB, Kasai N, Ferreira F, Faccini JL, Gennari SM.Natural tick infestations were assessed every 14 days on horses over a 2-year period. Amblyomma cajennense adult ticks were counted individually, without detachment from the horses. Larvae and nymphs of A. cajennense were collected using a rubber scraper that scratched engorged immature ticks from the host. Adult females of Anocentor nitens larger than 4mm length were counted on the horses. Blood samples were also obtained from the horses every 14 days and macroclimatic data were obtained for the study period. Infestations of A. cajennense demonstrated distinct peaks of activity for each of th... Read More
53
Occurrence of Leishmania sp. in cutaneous lesions of horses in Central Europe.
Veterinary parasitology
September 15, 2009
Volume 166, Issue 3-4 346-351 doi: 10.1016/j.vetpar.2009.09.001
Müller N, Welle M, Lobsiger L, Stoffel MH, Boghenbor KK, Hilbe M, Gottstein B, Frey CF, Geyer C, von Bomhard W.The present report describes a novel etiological agent of cutaneous leishmaniasis in horses that, at least for some cases, sporadically appeared as autochthonous infections in geographically distant regions of Germany and Switzerland. The infection was initially diagnosed upon clinical and immunohistological findings. Subsequent comparative sequence analysis of diagnostic PCR products from the internal transcribed spacer 1 (ITS1) of ssrRNA classified the respective isolates as neither Old World nor New World Leishmania species. However, four isolates subjected to molecular analyses all exhibit... Read More
53
An autochthonous case of cutaneous bovine leishmaniasis in Switzerland.
Veterinary parasitology
January 25, 2010
Volume 169, Issue 3-4 408-414 doi: 10.1016/j.vetpar.2010.01.022
Lobsiger L, Müller N, Schweizer T, Frey CF, Wiederkehr D, Zumkehr B, Gottstein B.The present case report describes a novel etiological agent of cutaneous leishmaniasis that appears for the first time in a cow. A similar agent had recently been described as causing autochthonous infections in horses of Germany and Switzerland. The infection in the cow was initially diagnosed upon clinical and immunohistological findings. Subsequent comparative sequence analysis of diagnostic PCR products from the internal transcribed spacer 1 (ITS1) of ssrRNA classified the respective isolate as neither Old World nor New World Leishmania species, but yielded complete identity of the analyse... Read More
52
Sequence heterogeneity in the 18S rRNA gene within Theileria equi and Babesia caballi from horses in South Africa.
Veterinary parasitology
October 11, 2008
Volume 159, Issue 2 112-120 doi: 10.1016/j.vetpar.2008.10.004
Bhoora R, Franssen L, Oosthuizen MC, Guthrie AJ, Zweygarth E, Penzhorn BL, Jongejan F, Collins NE.A molecular epidemiological survey of the protozoal parasites that cause equine piroplasmosis was conducted using samples collected from horses and zebra from different geographical locations in South Africa. A total of 488 samples were tested for the presence of Theileria equi and/or Babesia caballi using the reverse line blot hybridization assay. Ten percent of the samples hybridized to the Theileria/Babesia genus-specific probe and not to the B. caballi or T. equi species-specific probes, suggesting the presence of a novel species or genotype. The small subunit of rRNA gene (18S; approximat... Read More
51
Development of a single-round and multiplex PCR method for the simultaneous detection of Babesia caballi and Babesia equi in horse blood.
Veterinary parasitology
April 9, 2005
Volume 129, Issue 1-2 43-49 doi: 10.1016/j.vetpar.2004.12.018
Alhassan A, Pumidonming W, Okamura M, Hirata H, Battsetseg B, Fujisaki K, Yokoyama N, Igarashi I.With the aim of developing more simple diagnostic alternatives, a differential single-round and multiplex polymerase chain reaction (PCR) method was designed for the simultaneous detection of Babesia caballi and Babesia equi, by targeting 18S ribosomal RNA genes. The multiplex PCR amplified DNA fragments of 540 and 392 bp from B. caballi and B. equi, respectively, in one reaction. The PCR method evaluated on 39 blood samples collected from domestic horses in Mongolia yielded similar results to those obtained from confirmative PCR methods that had been established earlier. Thus, the single-roun... Read More
49
Cases of reduced cyathostomin egg-reappearance period and failure of Parascaris equorum egg count reduction following ivermectin treatment as well as survey on pyrantel efficacy on German horse farms.
Veterinary parasitology
November 16, 2006
Volume 144, Issue 1-2 74-80 doi: 10.1016/j.vetpar.2006.09.036
von Samson-Himmelstjerna G, Fritzen B, Demeler J, Schürmann S, Rohn K, Schnieder T, Epe C.In 2003 and 2004, on a total of 63 different German horse farms, a survey using the faecal egg count reduction (FECR) test was performed to investigate the efficacy of ivermectin (IVM, Ivomec) and pyrantel (PYR, Banminth) treatment against gastro-intestinal nematodes in a total of 767 horses. IVM treatment resulted in 100% reduction of the cyathostomin egg production 14 and 21 days post-treatment (d.p.t.) on 37 farms. On the remaining five farms, the mean faecal egg count reduction ranged between 97.7 and 99.9%. The mean cyathostomin FECR following PYR treatment ranged between 92.2 and 100% on... Read More
49
Microsporidia and Cryptosporidium in horses and donkeys in Algeria: detection of a novel Cryptosporidium hominis subtype family (Ik) in a horse.
Veterinary parasitology
January 19, 2015
Volume 208, Issue 3-4 135-142 doi: 10.1016/j.vetpar.2015.01.007
Laatamna AE, Wagnerová P, Sak B, Květoňová D, Xiao L, Rost M, McEvoy J, Saadi AR, Aissi M, Kváč M.A total of 219 and 124 individual fecal samples of horses and donkeys, respectively, were screened for the presence of Cryptosporidium spp., Encephalitozoon spp., and Enterocytozoon bieneusi DNA by genus-specific nested PCR. Isolates were genotyped by sequence analysis of SSU rRNA, GP60, TRAP-C1, COWP, and HSP70 loci in Cryptosporidium, and the ITS region in microsporidia. Cryptosporidium spp. was detected on 3/18 horse farms and 1/15 farms where donkeys were kept. Overall, five (2.3%) horse and two (1.6%) donkey specimens were PCR positive for Cryptosporidium. Genotyping at SSU and GP60 loci ... Read More
46
Analysis of multiyear studies in horses in Kentucky to ascertain whether counts of eggs and larvae per gram of feces are reliable indicators of numbers of strongyles and ascarids present.
Veterinary parasitology
August 17, 2010
Volume 174, Issue 1-2 77-84 doi: 10.1016/j.vetpar.2010.08.007
Nielsen MK, Baptiste KE, Tolliver SC, Collins SS, Lyons ET.Increasing levels of anthelmintic resistance in equine nematodes have led to recommendations of more sustainable anthelmintic treatment protocols with emphasis on parasite surveillance and diagnosis, rather than prophylactic calendar-based treatments. This requires knowledge of the diagnostic test performance of techniques for counts of eggs per gram of feces (EPG) as well as methods for culturing, counting and identifying third stage (L(3)) strongyle larvae per gram of feces (LPG). For horses, such information does not exist in the published literature. The aim of this study was to examine th... Read More
45
Giardia and Cryptosporidium in Canadian farm animals.
Veterinary parasitology
March 1, 1997
Volume 68, Issue 4 375-381 doi: 10.1016/s0304-4017(96)01072-2
Olson ME, Thorlakson CL, Deselliers L, Morck DW, McAllister TA.Giardia intestinalis and Cryptosporidium spp. are commonly identified intestinal pathogens in humans and animals. In light of the clinical disease, production losses and zoonotic potential of both Giardia and Cryptosporidium infections, a study was undertaken to investigate the prevalence of these parasites in cattle, sheep, pigs and horses in Canadian farms at different geographical locations. A total of 104 cattle, 89 sheep, 236 pigs and 35 horses were sampled from 15 different Canadian geographical locations. Fecal samples were examined after concentration and immunofluorescent staining. Gi... Read More
44
A review of Sarcocystis neurona and equine protozoal myeloencephalitis (EPM).
Veterinary parasitology
February 27, 2001
Volume 95, Issue 2-4 89-131 doi: 10.1016/s0304-4017(00)00384-8
Dubey JP, Lindsay DS, Saville WJ, Reed SM, Granstrom DE, Speer CA.Equine protozoal myeloencephalitis (EPM) is a serious neurological disease of horses in the Americas. The protozoan most commonly associated with EPM is Sarcocystis neurona. The complete life cycle of S. neurona is unknown, including its natural intermediate host that harbors its sarcocyst. Opossums (Didelphis virginiana, Didelphis albiventris) are its definitive hosts. Horses are considered its aberrant hosts because only schizonts and merozoites (no sarcocysts) are found in horses. EPM-like disease occurs in a variety of mammals including cats, mink, raccoons, skunks, Pacific harbor seals, p... Read More
43
Serologic and molecular characterization of Anaplasma species infection in farm animals and ticks from Sicily.
Veterinary parasitology
July 27, 2005
Volume 133, Issue 4 357-362 doi: 10.1016/j.vetpar.2005.05.063
de la Fuente J, Torina A, Caracappa S, Tumino G, Furlá R, Almazán C, Kocan KM.Although Anaplasma marginale was known to be endemic in Italy, the diversity of Anaplasma spp. from this area have not been characterized. In this study, the prevalence of Anaplasma spp. antibodies in randomly selected farm animals collected on the island of Sicily was determined by use of a MSP5 cELISA for Anaplasma spp. and an immunofluorescence test specific for Anaplasma phagocytophilum. Genetic variation among strains of Anaplasma spp. from animals and ticks was characterized using the A. marginale msp1alpha and the Anaplasma spp. msp4 genes. Eight species of ticks were collected and test... Read More
42
Enzootiology of Trypanosoma evansi in Pantanal, Brazil.
Veterinary parasitology
October 16, 2004
Volume 125, Issue 3-4 263-275 doi: 10.1016/j.vetpar.2004.07.013
Herrera HM, Dávila AM, Norek A, Abreu UG, Souza SS, D'Andrea PS, Jansen AM.In order to better understand the enzootiology of trypanosomiasis caused by Trypanosoma evansi in the Brazilian Pantanal we examined domestic and wild mammals by microhematocrit centrifuge technique (MHCT), immunofluorescence antibody test (IFAT) and polymerase chain reaction (PCR). T. evansi infection was detected in all species sampled with exception of the sheep and the feral pig. High parasitemias were observed in capybaras (5/24), coatis (18/115), horses (31/321) and dogs (3/112). Among these species, only the capybaras did not develop anemia. Low parasitemias, only detected by PCR, were ... Read More
41
Recent advances in leishmaniosis in pet animals: epidemiology, diagnostics and anti-vectorial prophylaxis.
Veterinary parasitology
April 19, 2011
Volume 181, Issue 1 23-30 doi: 10.1016/j.vetpar.2011.04.019
Gramiccia M.The leishmanioses are diseases caused by protozoa of the genus Leishmania, parasites infecting numerous mammal species, including humans, and transmitted by the bite of phlebotomine sand flies. They are a large group of diseases ranging over inter-tropical zones of America and Africa, and extend into temperate regions of Latin America, Europe and Asia. Pet animals are found infected with different Leishmania species but Leishmania infantum is the most widespread being dogs the main reservoir of zoonotic visceral leishmaniosis (ZVL). Dogs are very susceptible to this parasite and may suffer fro... Read More
41
Sensitive and specific detection of Cryptosporidium species in PCR-negative samples by loop-mediated isothermal DNA amplification and confirmation of generated LAMP products by sequencing.
Veterinary parasitology
September 11, 2008
Volume 158, Issue 1-2 11-22 doi: 10.1016/j.vetpar.2008.09.012
Bakheit MA, Torra D, Palomino LA, Thekisoe OM, Mbati PA, Ongerth J, Karanis P.Three LAMP (loop-mediated isothermal DNA amplification) assays were applied to detect Cryptosporidium species DNA in a total number of 270 fecal samples originating from cattle, sheep and horses in South Africa. DNA was extracted from 0.5 g of fecal material. Results of LAMP detection were compared to those obtained by nested PCR targeting the Cryptosporidium 18 small subunit rRNA (18S) gene. All samples were negative by nested PCR, while up to one-third of samples were positive by LAMP assays. The SAM-1 LAMP assay, shown to detect C. parvum, C. hominis and C. meleagridis, amplified Cryptospor... Read More
41
Detection and identification of equine Theileria and Babesia species by reverse line blotting: epidemiological survey and phylogenetic analysis.
Veterinary parasitology
July 22, 2004
Volume 123, Issue 1-2 41-54 doi: 10.1016/j.vetpar.2004.04.010
Nagore D, García-Sanmartín J, García-Pérez AL, Juste RA, Hurtado A.Specific oligonucleotide probes were designed to develop a new and highly sensitive reverse line blot assay to detect and identify simultaneously different Theileria and Babesia species in horses. The amplified hypervariable V4 region of the 18S rRNA gene was hybridised against different generic and species-specific probes. The survey was conducted over 243 samples of equine blood divided into three different groups: group 1, 24 horses presented as possible clinical piroplasmosis; group 2, 181 clinically healthy free-ranging horses exposed to ticks; group 3, 38 riding horses with unrelated pat... Read More
38
Genetic characterization of Cryptosporidium in animal and human isolates from Jordan.
Veterinary parasitology
August 21, 2016
Volume 228 116-120 doi: 10.1016/j.vetpar.2016.08.015
Hijjawi N, Mukbel R, Yang R, Ryan U.Little is known about the epidemiology of Cryptosporidium in Jordan and to date, only one genotyping study has been conducted on Cryptosporidium isolates from Jordanian children. In the present study, a total of 284 faecal samples from Jordanian cattle, sheep, goats and chicken and 48 human faecal samples were screened for the presence of Cryptosporidium using an 18S quantitative PCR (qPCR) and a C. parvum/C. hominis specific qPCR at a lectin locus. Of these, 37 of 284 animal faecal samples were positive by qPCR at the 18S locus giving an overall prevalence of 11.6%. The point prevalence of Cr... Read More
38
The management of anthelmintic resistance in grazing ruminants in Australasia–strategies and experiences.
Veterinary parasitology
December 31, 2013
Volume 204, Issue 1-2 44-54 doi: 10.1016/j.vetpar.2013.12.022
Leathwick DM, Besier RB.In many countries the presence of anthelmintic resistance in nematodes of small ruminants, and in some cases also in those infecting cattle and horses, has become the status quo rather than the exception. It is clear that consideration of anthelmintic resistance, and its management, should be an integral component of anthelmintic use regardless of country or host species. Many years of research into understanding the development and management of anthelmintic resistance in nematodes of small ruminants has resulted in an array of strategies for minimising selection for resistance and for dealin... Read More
36
Risk factors to tick infestations and their occurrence on horses in the state of São Paulo, Brazil.
Veterinary parasitology
May 5, 2001
Volume 97, Issue 1 1-14 doi: 10.1016/s0304-4017(01)00387-9
Labruna MB, Kerber CE, Ferreira F, Faccini JL, De Waal DT, Gennari SM.From December 1998 to March 1999, 40 stud farms were studied in the state of São Paulo, Brazil. During visits to farms, horses reared under grazing conditions were examined for the presence of ticks. On each farm visit, horse pastures were closely inspected and a questionnaire was given to the farm supervisor with the purpose of gaining information about ecological and management variables (independent variables) that could be associated with the presence and infestation levels of ticks on the farm (dependent variables). Three tick species were found during the study. Anocentor nitens, Amblyo... Read More
36