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Topic:Diagnostic Technique

Diagnostic techniques in equine medicine encompass a range of procedures and tools used to identify diseases, injuries, or other health conditions in horses. These techniques include imaging methods such as radiography, ultrasonography, and magnetic resonance imaging (MRI), as well as laboratory tests like blood work and tissue biopsies. Each diagnostic method provides specific information that can aid in the assessment and management of equine health issues. Radiography is commonly used for evaluating bone structures, while ultrasonography is useful for soft tissue examination. MRI offers detailed images of both soft and hard tissues, though its use is limited by cost and availability. Laboratory tests can detect biochemical and hematological changes indicative of disease. This page compiles peer-reviewed research studies and scholarly articles that explore the development, application, and efficacy of various diagnostic techniques in equine veterinary practice.
Ultrasound as an aid to diagnosis of granulosa cell tumour in the mare.
Equine veterinary journal    March 1, 1990   Volume 22, Issue 2 99-103 doi: 10.1111/j.2042-3306.1990.tb04218.x
Hinrichs K, Hunt PR.The ultrasonic appearance of seven cases of granulosa cell tumour is described, and compared with an ovarian haematoma and an ovarian serous cystadenoma. The granulosa cell tumours varied from being uniformly dense to having one or several large fluid filled cysts. Some tumours resembled the haematoma or cystadenoma and also, in some aspects, normal ovarian structures such as corpora haemorrhagica or follicles in early pregnancy. There was no typical ultrasonographic appearance of the granulosa cell tumours which enabled definitive diagnosis. However, ultrasound may be a diagnostic aid when us...
Upper airway function during maximal exercise in horses with obstructive upper airway lesions. Effect of surgical treatment.
Veterinary surgery : VS    March 1, 1990   Volume 19, Issue 2 142-147 doi: 10.1111/j.1532-950x.1990.tb01156.x
Williams JW, Meagher DM, Pascoe JR, Hornof WJ.Upper airway pressure was measured during maximal exercise in 10 Thoroughbred racehorses with naturally occurring upper airway obstruction. Left laryngeal hemiplegia and arytenoid chondropathy resulted in substantial increases (30-40 cm H2O) in inspiratory upper airway pressure (Pl), whereas complicated aryepiglottic entrapment and subepiglottic cysts produced only modest increases (15 cm H2O) in Pl. Uncomplicated aryepiglottic entrapment and grade IV pharyngeal lymphoid hyperplasia produced only slight increases (3-5 cm H2O). In general, surgical procedures restored airway pressures to within...
Surgical repair of tibial tuberosity avulsion fractures in four horses.
Veterinary surgery : VS    March 1, 1990   Volume 19, Issue 2 117-121 doi: 10.1111/j.1532-950x.1990.tb01151.x
Smith BL, Auer JA, Watkins JP.Four horses, 2 to 17 years old, were treated for unilateral avulsion fractures of the tibial tuberosity. Two horses were treated successfully with tension band wiring or plating in combination with lag screw fixation. One horse was euthanatized because of implant failure during recovery and one was euthanatized on day 11 because a longitudinal fracture of the tibial tuberosity occurred through the plane of the screws used for stabilization.
Changes in plasma progesterone concentrations from days 17 to 42 of gestation in mares maintaining or losing pregnancy.
Equine veterinary journal    March 1, 1990   Volume 22, Issue 2 104-106 doi: 10.1111/j.2042-3306.1990.tb04219.x
Irvine CH, Sutton P, Turner JE, Mennick PE.Plasma progesterone concentrations were measured in 179 mares bled on alternate days commencing with a positive pregnancy diagnosis on Days 17 to 18 after ovulation and concluding on Days 42 to 45. During this period 17 mares (10 per cent) lost their pregnancies, 11 before Day 25. In 15 mares the timing of the pregnancy loss could be determined with adequate accuracy; in only one did a decline in progesterone precede the loss. Thus pregnancy loss between Days 17 and 42 was rarely caused by a fall in plasma progesterone.
Impedance plethysmography.
Equine veterinary journal    March 1, 1990   Volume 22, Issue 2 114-117 doi: 10.1111/j.2042-3306.1990.tb04221.x
Attenburrow DP, Flack FC, Portergill MJ.The technique of impedance plethysmography is described and its application to observation of lung volume changes in the horse at exercise is discussed. The results from horse at rest show that there is a close relationship between rate of lung volume change (flow rate) and the associated impedance changes during both inspiration and expiration. Impedance changes during exercise were related to inspiration and expiration by observation of associated respiratory sounds. Artefacts related to technical difficulties are also indicated.
Use of gonadotropin-releasing hormone for hastening ovulation in transitional mares.
Journal of animal science    March 1, 1990   Volume 68, Issue 3 690-699 doi: 10.2527/1990.683690x
Harrison LA, Squires EL, Nett TM, McKinnon AO.Natural GnRH and its analog have potential for hastening ovulation in mares. A study was conducted to evaluate the efficacy of a GnRH agonist given either as an injectable or s.c. implant for induction of ovulation in mares. Forty-five seasonally anestrous mares (March) were assigned to one of three groups (n = 15/group): 1) untreated controls; 2) i.m. injection of the GnRH agonist buserelin at 12-h intervals (40 micrograms/injection for 28 d or until ovulation) and 3) GnRH agonist administered as a s.c. implant (approximately 100 micrograms/24 h for 28 d). Six mares per group were bled on d 0...
Lysozyme, alkaline phosphatase and neutrophils in uterine secretions of mares with differing resistance to endometritis.
Theriogenology    March 1, 1990   Volume 33, Issue 3 723-732 doi: 10.1016/0093-691x(90)90549-9
Katila T, Lock TF, Hoffmann WE, Smith AR.A study was conducted to 1) determine differences in the inflammatory response following bacterial challenge between normal mares and mares with chronic endometritis and 2) to determine if enzyme activity in uterine fluid can be used to evaluate degree of inflammation in the equine uterus. Six normal mares (Group 1) and four mares with chronic endometritis (Group 2) received an intrauterine infusion of beta-hemolytic streptococci on the second day of estrus. Neutrophil concentration as well as lysozyme and alkaline phosphatase activity were determined in uterine secretions obtained by placing ...
Effects of left recurrent laryngeal neurectomy, prosthetic laryngoplasty, and subtotal arytenoidectomy on upper airway pressure during maximal exertion.
Veterinary surgery : VS    March 1, 1990   Volume 19, Issue 2 136-141 doi: 10.1111/j.1532-950x.1990.tb01155.x
Williams JW, Pascoe JR, Meagher DM, Hornof WJ.Upper airway pressure was measured with a nasotracheal catheter system and a portable pressure transducer in 10 normal horses during maximal exercise before and after left recurrent laryngeal neurectomy. Measurements were repeated 16 weeks after prosthetic laryngoplasty (5 horses) or subtotal arytenoidectomy (5 horses). During maximal exertion, prosthetic laryngoplasty was more effective than subtotal arytenoidectomy in reversing the increases in upper airway pressure that followed left recurrent laryngeal neurectomy.
Scanning electron and light microscopy of the mucosa of the equine ileocaecal junction.
The Onderstepoort journal of veterinary research    March 1, 1990   Volume 57, Issue 1 19-23 
Kotzé SH, Soley JT.The ileocaecal junction mucosal surface morphology of 5 horses and 1 donkey was examined using scanning electron microscopy and light microscopy. Based on differences in surface morphology, the junction could be divided into 4 distinct regions. The distal extremity of the ileum was characterized by the presence of typical finger-like villi followed by a narrow zone displaying short, stubby villi which gradually merged into a 3rd region representing the true transitional zone between the small and large intestine. In this region the villi fused, forming low ridges arranged in circular, semi-cir...
Arthroscopic approaches and intraarticular anatomy of the equine elbow.
Veterinary surgery : VS    March 1, 1990   Volume 19, Issue 2 93-101 doi: 10.1111/j.1532-950x.1990.tb01148.x
Nixon AJ.Periarticular anatomy and techniques for arthroscopic access to the equine elbow were studied in six joints from cadavers. Caudomedial and craniolateral approaches were evaluated subsequently in 11 anesthetized horses. The caudomedial approach was made between the flexor carpi radialis and flexor carpi ulnaris muscle bellies. Most of the caudal articular surfaces of the humeral condyles, the caudal perimeter of the radius, and the trochlear notch and portions of the anconeal process of the ulna could be identified. The voluminous caudal joint capsule cul-de-sac proximal to the anconeal process...
Effect of age on sensory nerve conduction velocity in the horse.
Research in veterinary science    March 1, 1990   Volume 48, Issue 2 141-144 
Wheeler SJ.This study aimed to establish a clinically reproducible method of evaluation of sensory nerve conduction in the horse and to provide reference values in a group of normal horses. Age-related changes in the sensory nerve conduction velocity were of particular interest. Sensory nerve conduction was performed in the lateral palmar nerve. The results revealed an increase in velocity over the first year of life and a subsequent decrease in velocity in older horses. The effect of age must be considered if results from horses in which peripheral nerve disease is suspected are to be interpreted correc...
Morphology and differential diagnostics of parasitic larvae of some Strongylidae (Nematoda) of horses.
Angewandte Parasitologie    February 1, 1990   Volume 31, Issue 1 15-28 
Dvojnos GM, Harcenko VA.The morphology of L4 of Cylicostephanus minutus, Cylicotetrapedon bidentatus, Petrovinema poculatum, Cylicocyclus radiatus, C. triramosus, Cylicodontophorus euproctus, C. mettami, C. mongolicus, Poteriostomum imparidentatum, Gyalocephalus capitatus and Craterostomum acuticaudatum is discussed. The species are named in accordance with the new system, which is outlined in the introduction. The criteria for the identification of larvae killed during their 4th skinning include distinctive marks occuring in the 4th and 5th stages: gut morphology, position of cervical papillae, excretory opening, mo...
ECG of the month. Premature ventricular complexes in an anesthetized colt.
Journal of the American Veterinary Medical Association    February 1, 1990   Volume 196, Issue 3 420-422 
Cornick JL, Hartsfield SM, Miller M.No abstract available
Endometrial concentrations of ampicillin in mares after intrauterine infusion of the drug.
American journal of veterinary research    February 1, 1990   Volume 51, Issue 2 197-199 
Love CC, Strzemienski PJ, Kenney RM.Serum concentration of ampicillin, a semisynthetic penicillin, was measured in mares at various time intervals up to 24 hours after intrauterine infusion of 3 g of ampicillin. Blood samples were drawn immediately before infusion and at 1-, 4-, 10- and 24-hour intervals after infusion. At postinfusion hour 24, two endometrial biopsy specimens were obtained to measure endometrial concentrations of ampicillin. Blood was drawn twice as part of the 24-hour postinfusion sample collection, once before removal of the biopsy specimens and again 5 minutes after removal of the biopsy specimens. After dru...
Evaluation of upper respiratory tract function during strenuous exercise in racehorses.
Journal of the American Veterinary Medical Association    February 1, 1990   Volume 196, Issue 3 431-438 
Morris EA, Seeherman HJ.Forty-six racehorses with a history of poor performance underwent endoscopic evaluation of laryngeal and pharyngeal function while exercising on a high-speed treadmill. This evaluation allowed the definitive diagnosis of intermittent or continual upper respiratory tract obstruction as a cause of poor performance, as well as the documentation of the dynamic functional anatomy of the obstruction. Ten of the horses (22%) were determined to have a functional abnormality of the upper respiratory tract. These abnormalities included epiglottic entrapment (1 horse), persistent dorsal displacement of t...
Autoimmune haemolytic anaemia in eight horses.
The Veterinary record    January 20, 1990   Volume 126, Issue 3 51-53 
Mair TS, Taylor FG, Hillyer MH.Autoimmune haemolytic anaemia was diagnosed in eight horses on the basis of a positive Coomb's test. The disease was considered to be idiopathic in three cases and secondary to another condition in five. The clinical signs included dullness and depression, pyrexia, jaundice and haemoglobinuria. In addition to anaemia, haematological evaluation commonly revealed auto-agglutination of red cells and anisocytosis; reticulocytosis and an increased mean corpuscular volume were less commonly observed. Four horses were successfully treated with dexamethasone, but the other four were destroyed or died ...
Diagnostic ultrasonography for evaluation of cryptorchidism in horses.
Journal of the American Veterinary Medical Association    January 15, 1990   Volume 196, Issue 2 297-300 
Jann HW, Rains JR.The location and size of 11 retained testes were accurately determined ultrasonographically. There was 100% correlation between the location of the testis determined by ultrasound vs that determined by surgery. Testicular size determined presurgically in all cases closely approximated the actual size obtained by gross measurement of the excised testis. The cryptorchid testicular echotexture was less dense than that of the normal descended testicles, but was easily identified. Ultrasonographic evaluations were completed by use of an ultrasound base unit with attached 5-MHz transrectal transduce...
Radiographic and scintigraphic imaging of a proximal radial physeal injury in a young horse induced by olecranon fracture repair.
Equine veterinary journal    January 1, 1990   Volume 22, Issue 1 56-59 doi: 10.1111/j.2042-3306.1990.tb04209.x
Metcalf MR, Tate LP, Sellett LC, Henry M.No abstract available
[3-dimensional movement analysis as a clinical method of objective assessment of lameness in the horse].
Biomedizinische Technik. Biomedical engineering    January 1, 1990   Volume 35 Suppl 2 171-172 doi: 10.1515/bmte.1990.35.s2.171
Kastner J, Knezevic PF, Girtler D, Toeltsch M.No abstract available
Is there a morning-evening difference in egg production of bloodworms, strongyle parasites of equines?
Progress in clinical and biological research    January 1, 1990   Volume 341B 729-733 
Bennett MF.To test a claim that counts of the eggs of large strongyles or bloodworms, parasites of equines, are greater in morning fecal samples than in those from later in the day, and therefore, may be indicators of circadian variations in these worms, samples from one pony and nine horses were analyzed for strongyle eggs. Samples were collected twice per day, about 7:30 and again about 19:30, during two, 24-day periods. Individual counts varied from 0 to 1184. The mean of all 7:30 counts was 212.52; that of 19:30 counts was 236.03. The difference is not significant, and does not support the idea of ci...
Correction for skin displacement errors in movement analysis of the horse.
Journal of biomechanics    January 1, 1990   Volume 23, Issue 1 97-101 doi: 10.1016/0021-9290(90)90374-c
van den Bogert AJ, van Weeren PR, Schamhardt HC.In movement analysis of the horse, large errors result from movements of the skin with respect to the underlying bones. A generally applicable, two-dimensional, method for correction of these skin-movement errors in kinematic data has been developed. It was tested on a kinematic analysis of the hindlimb in a walking pony. The results indicate that without correction for skin-movement errors, misreadings of up to 15 degrees in the knee angle and 30% in the moment arm of the gastrocnemius muscle can be expected.
[A rapid isolation of Venezuelan equine encephalomyelitis virus using the lanthanide immunofluorescence assay].
Voprosy virusologii    January 1, 1990   Volume 35, Issue 1 77-79 
Kharitonenkov IG, Gaĭdamovich SIa, Pomelova VG, Sokolova MV, Lavrova NA, Leonov SV, Zlobin VN.No abstract available
Primary cholangiohepatitis in a horse.
The Cornell veterinarian    January 1, 1990   Volume 80, Issue 1 35-40 
Schulz KS, Simmons TR, Johnson R.An 8-year-old mare was presented to the New York State College of Veterinary Medicine Large Animal Clinic for evaluation of anorexia, fever and icterus. The mare had a 5-day history of anorexia, depression and tongue protrusion. Diagnostic procedures included serum hepatic enzyme activities, serum bile acid concentrations, blood ammonia evaluations and hepatic ultrasound and ultrasound guided biopsy. The history, clinical pathology and histopathology in this case supported a probable diagnosis of primary septic cholangiohepatitis.
Mycoplasmas from donkeys and horses in the Sudan.
Revue d'elevage et de medecine veterinaire des pays tropicaux    January 1, 1990   Volume 43, Issue 3 323-324 
Shams el Din HE, el Nasri M.Seventeen isolates (4.27%) were recovered from 398 samples. Twelve isolates (4%) were obtained from 300 donkey nasal swabs, three (4.3%) and two (6.89%) isolates were recovered from 69 horse nasal swabs and 29 mare uterine washings, respectively. Nine isolates were lost during storage at -20 degrees C and the remaining eight were identified as mycoplasmas and their biological, biochemical and serological reactions were investigated. The isolates could be divided into two groups on the basis of glucose fermentation and arginine hydrolysis. The first group neither fermented glucose nor hydrolyse...
Osteostixis for incomplete cortical fracture of the third metacarpal bone. Results in 11 horses.
Veterinary surgery : VS    January 1, 1990   Volume 19, Issue 1 34-40 doi: 10.1111/j.1532-950x.1990.tb01140.x
Specht TE, Colahan PT.Eleven horses with acute or chronic incomplete cortical fractures of the left or right third metacarpal bone (McIII) were treated with surgical puncture (osteostixis). The fractures were diagnosed by physical examination and radiography. Four to eight holes, 2.7 or 3.5 mm in diameter, were drilled in the fractured bone. Radiographically, the fractures were healed by month 3, and the drill holes were inapparent by month 7. Nine horses (82%) returned to race competition, and two horses were retired, one the result of a surgical complication. The mean time between surgery and the first race was 9...
Allergens of horse epithelium. I. Physicochemical and immunochemical characterization of five different horse epithelium raw materials used for allergen extract preparation.
International archives of allergy and applied immunology    January 1, 1990   Volume 92, Issue 3 309-317 
Franke D, Maasch HJ, Wahl R, Schultze-Werninghaus G, Bretting H.We investigated five horse epithelial allergen extracts prepared from different qualities of raw material by several biochemical and immunochemical methods. Horse serum albumin and horse serum were used to identify serum-related antigens. We found high similarities as well as marked differences between the extracts. There were strong differences in the protein contents, the protein patterns obtained by isoelectric focusing and sodium dodecyl sulfate polyacrylamide gel electrophoresis, the total allergenic activities obtained by radioallergosorbent test inhibition assays and the amounts and num...
Further evidence for a silent allele in the transferrin locus of the horse.
Animal genetics    January 1, 1990   Volume 21, Issue 4 423-426 doi: 10.1111/j.1365-2052.1990.tb01987.x
Schmid DO, Ek N, Braend M.A silent allele in the transferrin locus (Tf) was observed in a Thoroughbred mare and in five of her offspring from three different matings. Evidence for the silent allele was obtained by quantitative immunodiffusion studies.
Population data and a fourth allele for equine complement component 3 (C3).
Animal genetics    January 1, 1990   Volume 21, Issue 1 83-86 doi: 10.1111/j.1365-2052.1990.tb03210.x
Bowling AT, Dileanis S.The C3 polymorphism of equine serum or plasma revealed by agarose gel electrophoresis can be diagnosed with protein stain following acid protein fixation. In addition to the three alleles previously described (C31, C32, C33), a fourth allele (C34) was found. Population data for 25 domestic breeds and Equus przewalskii are presented.
An improved method for the study of equine haptoglobin heterogeneity.
Veterinary research communications    January 1, 1990   Volume 14, Issue 6 433-439 doi: 10.1007/BF00367054
Milne EM.Equine serum haptoglobin was separated by polyacrylamide gel isoelectric focusing and visualized by protein staining or Western blotting. Conventional protein staining revealed up to three bands in the pI range 4.17 to 4.44. The blotting technique, however, showed an anodal group of 8 to 10 bands with a pI range of 4.11 to 4.52 and a cathodal group of 4 to 6 bands with a range of 4.55 to 5.14. The blotting method revealed that equine haptoglobin migrates outside the prealbumin area, in contrast to previous reports.
[Experiences with the QBC-V hematology system at the veterinary hospitals of Zurich and Bern].
Schweizer Archiv fur Tierheilkunde    January 1, 1990   Volume 132, Issue 5 261-266 
Meister D, Tschudi P, Hermann M, Lutz H.The QBC V hematology system was tested with respect to its application in veterinary medicine. PCV's and counts of total leukocytes, granulocytes, lympho/monocytes and platelets collected from 435 horses, dogs and cats were determined and compared with conventionally measured values. Precision and accuracy were found to be good for the majority of parameters. In the authors' opinion the QBC V hematology system is well suited for use in veterinary practice.