Topic:Fertility
Fertility in horses encompasses the physiological processes and factors influencing reproductive success in equine species. It involves the study of reproductive anatomy, endocrinology, and behavior, as well as the management practices that affect breeding outcomes. Key aspects include the estrous cycle, conception rates, and factors impacting stallion and mare fertility. Reproductive technologies such as artificial insemination, embryo transfer, and hormonal therapies are also explored to enhance breeding efficiency. This page compiles peer-reviewed research studies and scholarly articles that examine the biological mechanisms, management strategies, and technological advancements related to equine fertility.
Evaluation of the expression of a male-specific antigen on cells of equine blastocysts. This experiment was designed (1) to determine if H-Y antigen is expressed on the cell surface of pre-implantation equine blastocyst stage embryos, (2) if so, to identify differences in expression on inner cell mass (ICM) verses trophectoderm cells and (3) to evaluate whether the detection of this glycoprotein would aid in the identification of equine embryonic sex. A total of 33 blastocyst stage horse embryos were collected 6-7 days post-ovulation by trans-cervical flush and were immediately evaluated for the presence of H-Y antigen. Additionally, 17 embryos, collected at similar stages and cu...
Equine endometrial cytology. The simplicity of collection of material for cytologic preparations belies the complexity of smear interpretation beyond recognition of neutrophils. Knowledge concerning cancer cytology moves rapidly, for cancer is a progressive, often fatal disease so that tissue for comparison and confirmation of interpretation often becomes available. This is not true for cytologic study of the equine endometrium. Lesions detected by means of cytology smears may be transient and regress, offering little information concerning their etiology or consequences. They may be focal and missed in the corresponding ...
XY sex reversal syndrome in the mare: clinical and behavioral studies, H-Y phenotype. An inherited genetic disorder causes XY embryos of the horse to develop as mares. On the basis of our study of 38 such mares, we have identified four grades or classes of XY sex reversal according to this scheme: class I, nearly normal female, of which some are fertile; class II, female with gonadal dysgenesis, normal mullerian development; class III, intersex mare with gonadal dysgenesis, abnormal mullerian development, enlarged clitoris; class IV, virilized intersex characterized by high levels of testosterone. In general, class I and class II mares were typed H-Y antigen-negative whereas cl...
Control of the estrous cycle in the mare. All current approaches to manipulating the reproductive biology of the nonpregnant mare are discussed.
Embryonic loss in mares. Incidence, possible causes, and diagnostic considerations. Fertilization rates were similar for normal and subfertile mares, and much of the difference in fertility between normal and subfertile mares was due to embryonic loss. Fertilization rate estimates for mares ranged from 71 to 96 per cent. The incidence of embryonic loss detected by ultrasonography between Days 11 and 50 was approximately 9 per cent for normal mares, and the estimated incidence of embryonic loss before Day 14 was also 9 per cent. Therefore, the estimated incidence of embryonic loss in normal mares between fertilization and Day 50 is approximately 18 per cent (Fig. 1). In subfer...
Embryo reduction before day 11 in mares with twin conceptuses. Incidence of embryo reduction (natural elimination of one member of a twin set) before d 11 was studied by comparing the number of ultrasonically detected conceptuses per ovulation between single and double ovulators. Effect of unilateral (n = 24) vs bilateral (n = 26) double ovulations on the incidence of embryo reduction also was considered. Each of 50 double-ovulating mares was matched with two single ovulators yielding 100 ovulations, or potential embryos, per group. Frequency with which an ovulation resulted in a conceptus was greater for single ovulators (85%, P less than .01) and for bi...
Studies of stallion sperm survival: preservation of progressive motility of stallion spermatozoa by low ionic strength media. Preservation of stallion sperm forward motility was studied using a video recording system in semen diluted with media of different ionic strength and sodium content. After 8 hr of incubation at room temperature, semen diluted in a low ionic strength media containing sucrose displayed 65 +/- 9% motility with 68 +/- 3% of the motile sperm showing forward motility (diameter of head trajectory greater than or equal to 80 microns). In contrast, sperm populations diluted and incubated with a normal ionic strength media containing sodium had 56 +/- 7% motile sperm of which only 36 +/- 7% displayed f...
Secretion rates and short-term patterns of gonadotrophin-releasing hormone, FSH and LH in the normal stallion in the breeding season. Pituitary venous blood was collected by a painless nonsurgical cannulation method from five ambulatory stallions at 5-min intervals for 5-6 h during the breeding season. In four adult stallions, statistical analysis showed that pulses of gonadotrophin-releasing hormone (GnRH) and LH were coincident (P less than 0.01), as were pulses of FSH and LH (P less than 0.05). Furthermore, the patterns of changes in concentration of FSH and LH were highly correlated in each of the four stallions. However, seemingly ineffective pulses of GnRH were also observed, with 28% of GnRH pulses failing to induce a...
[Karyotypes of cattle (Bos taurus) and horses (Equus caballus) on the basis of synaptonemal complexes]. The cytogenetic study performed has shown that karyotyping of meiotic cells can be based on the synaptonemal complexes (SC) of spreading pachytene spermatocytes of bull and of horse. The horse SC karyotype has not been previously described. A comparison of the relative length of SC with metaphase chromosomes of bull and horse somatic cells has revealed the correspondence of the chromosome length in pachytene of meiosis and metaphase, which is in agreement with the data on house mouse and Chinese hamster. The method of spreading pachytene cells may be of great practical importance in studies of...
A 64XX/65XXX mosaic mare (Equus caballus) and associated infertility. IT is well known that chromosomal abnormalities are
frequently associated with infertility in the mare (Table 1). The
most commonly reported abnormality is 6 3 x 0 which can
occur in a mosaic or non-mosaic form. Various other
aneuploidies of the sex chromosomes have been reported but
the 6SXXX genotype appears to be rare. Chandley et a1 (1975)
and Chandley (1984) have reported two 65XXX animals and
Walker (1979) a 64XW6SXXW6SXXY one.
This paper reports a mosaic 64XXh5XXX animal and
emphasises the need for more cytogenetic analysis in the
horse.
Effect of insemination timing on the fertilizing capacity of frozen/thawed equine spermatozoa. A breeding trial was conducted to evaluate the effect of insemination timing on the fertility of mares bred with frozen/thawed equine semen. One stallion and 60 reproductively sound, estrous-synchronized mares were included in the study. Mares were assigned to one of three groups (n = 20): 1) insemination with fresh semen every other day during estrus from detection of a 35-mm follicle until ovulation, 2) insemination with frozen/thawed semen every day during estrus from detection of a 35-mm follicle until ovulation or 3) insemination with frozen/thawed semen once, within 6 h after ovulation. ...
Ultrasonographic studies on the reproductive tract of mares after parturition: effect of involution and uterine fluid on pregnancy rates in mares with normal and delayed first postpartum ovulatory cycles. During breeding of mares, ultrasonographic detection of uterine fluid accumulations in the first postpartum ovulatory period was associated with significantly decreased pregnancy rates, when compared with rates in control mares (P less than 0.005). The previously gravid uterine horn was recognized as the larger horn, when assessed for size by ultrasonography, for a mean of 21 days (range, 15 to 25 days) after parturition. On the basis of similar measurements obtained during 3 ultrasonographic scans (5-day period), uterine involution was determined to be completed in a mean of 23 days (range, 1...
24-Hour cooled storage of equine embryos. Equine embryos were collected by transcervical uterine flush 7 d after ovulation. The flush solution was Dulbecco's phosphate buffered saline (PBS) with 1% newborn calf serum and penicillin-streptomycin. Each embryo was washed in modified Dulbecco's PBS with 1% newborn calf serum and 0.4% bovine serum albumin, and placed in 4-ml polystyrene test tube containing this same medium. Embryos were packaged in a commercial semen transport container which cooled (-0.3 degrees C/min) and maintained the embryo at 4 to 6 degrees C. After 24 h, 16 embryos were transcervically transferred into recipient ma...
Effects of cooling rate and storage temperature on equine spermatozoal motility parameters. Two experiments were conducted to examine the effects of cooling rate and storage temperature on motility parameters of stallion spermatozoa. In Experiment 1, specific cooling rates to be used in Experiment 2 were established. In Experiment 2, three ejaculates from each of two stallions were diluted to 25 x 10(6) sperm/ml with 37 degrees C nonfat dry skim milk-glucose-penicillin-streptomycin seminal extender, then assigned to one of five treatments: 1) storage at 37 degrees C, 2) storage at 25 degrees C, 3) slow cooling rate to and storage at 4 degrees C, 4) moderate cooling rate to and storag...
Methods for collecting follicular oocytes from mares. A series of experiments was conducted to develop a procedure for consistent, repeatable collection of oocytes from the preovulatory follicle of the mare. In one experiment, in situ follicular aspiration with a needle and syringe was performed on 19 mares. From 37 aspirations, four oocytes were recovered (10% recovery rate). In a second experiment, ovaries were visualized via standing flank laparotomy during which two different aspiration techniques were used. Use of a needle and syringe as in the first experiment resulted in successful oocyte recovery in one of seven (14%) attempts. Aspiration...
ELA and fertility in American Standardbred horses. We have analysed the effects of ELA alleles and sire-dam ELA incompatibility on two measures of fertility, gestation length and foaling rate, in American Standardbred horses. Using multivariate statistical methods, we corrected for the effects of confounding factors such as dam and sire age, parity, inbreeding, and sire-dam kinship. These analyses revealed substantial differences between Standardbred trotters and pacers in the effects of several confounding factors. There appear to be no ELA effects on gestation length in either trotters or pacers. However our results suggest that there may be...
[Sperm received in shipment versus fresh sperm in relation to fertilization results]. The conception rates of semen intended for shipment and those of recently obtained semen are compared in the present paper. Conception rates using recently obtained semen were significantly superior to those obtained with semen intended for shipment. A number of factors to which this difference could be due are briefly discussed.
Comparison of Ham’s F10 with CO2 or Hepes buffer for storage of equine embryos at 5 C for 24 H. Forty equine embryos collected 7 d post-ovulation were stored at 5 C for 24 h in one of two culture media (n = 20/group): 1) Ham's F10 + 10% heat-treated fetal calf serum (FCS) buffered by gassing with 5% CO2, 5% O2 and 90% N2 and 2) Ham's F10 + 10% FCS with Hepes buffer (25 mM). Embryos cultured in Ham's F10 + CO2 maintained a better quality score and had a larger average increase in diameter (+34.8 micron) than embryos stored in Hepes buffered Ham's F10 (-10.2 micron). Embryos were transferred surgically into recipient mares that ovulated -3 to +1 d in relation to the donor mare. Twenty embr...
Effects of semen fractionation and dilution ratio on equine spermatozoal motility parameters. Two experiments were conducted to examine the effects of semen fractionation and dilution ratio on motility parameters of stallion spermatozoa. In Experiment 1, three ejaculates from each of three stallions were divided into sperm-rich (SR) and sperm-poor (SP) fractions to determine the difference in sperm concentration. Mean sperm concentration in SR fractions (349.5 x 10(6)/ml) was greater (P < 0.001) than that of SP fractions (96.9 x 10(6)/ml). In Experiment 2, three ejaculates from each of two stallions were divided into SR and SP fractions. Fifty percent of the original volume of SR fract...
The ovulated ovum of the horse: cytology of nonfertilized ova to pronuclear stage ova. Fertilization and early development in the horse were studied by recovering oviductal ova at various times after postovulatory mating. Ova collected between 7 and 22 h post coitum (pc) were examined for evidence of fertilizing sperm, cellular changes accompanying fertilization, and pronuclear development. Five ova collected between 7 and 9 h pc contained a marginal metaphase plate, but had no indication of sperm components; three of these, however, showed reduced numbers of cortical granules. Two activated ova (10 and 14 h pc) were in telophase of the second meiotic division, following incorpo...
Induction and characterization of acrosome reaction in equine spermatozoa. Equine spermatozoa were incubated in a chemically defined medium for 8 hours. The medium preserved spermatozoal viability, as assessed by total spermatozoal motility, progressive spermatozoal motility, and spermatozoal exclusion of eosin stain. Effects of time and divalent cation ionophore, A23187, on the occurrence and character of the spermatozoal acrosome reaction were determined. Two light microscopic assays, a triple-stain technique and a chlortetracycline fluorescence assay, were calibrated with transmission electron microscopy for detection of the acrosome reaction. Incubation time and ...
Secretion rates and short-term patterns of gonadotrophin-releasing hormone, FSH and LH throughout the periovulatory period in the mare. We have developed a non-surgical technique for long-term collection of pituitary venous blood which consists of slightly diluted hypophysial portal blood into which pituitary hormones have been secreted. In these experiments jugular and pituitary venous blood samples were collected from five unmedicated, ambulatory mares at 5-min intervals for 2-6 h on 11 occasions during the 6 days surrounding the ovulatory LH peak. Jugular blood only was collected from another five periovulatory mares without pituitary cannulae. The duration of oestrus was similar in mares with and without pituitary cannulae...