Reproduction in horses encompasses the biological processes and mechanisms involved in the breeding and development of equine offspring. This includes the study of reproductive anatomy, physiology, and endocrinology in both mares and stallions. Key areas of interest include the estrous cycle, ovulation, conception, gestation, and parturition. Researchers also examine factors influencing fertility, reproductive technologies such as artificial insemination and embryo transfer, and management practices that impact reproductive success. This page compiles peer-reviewed research studies and scholarly articles that explore the physiological, genetic, and environmental aspects of equine reproduction.
Papkoff H.The objectives of this paper are to review the chemical and biological properties of equine chorionic gonadotropin (eCG, PMSG) isolated from the serum. Comparisons are made with eCG isolated from endometrial cups, trophoblast cell culture medium, and low titer serum. The results show that eCG can vary, depending on the source, in both chemical and biological (LH and FSH activity) properties.
Bhavnani BR, Woolever CA.[3H]Equilin [3H-labeled 3-hydroxy-1,3,5(10), 7-estratetraen-17-one] was administered iv to a pregnant mare in the 10th month of gestation. Maternal urine was collected for 3 days, and blood samples were taken 35 min and 3, 6, 12, and 24 h after the injection. The half-life of the disappearance of radioactivity from the blood was approximately 2.5 h. Over 90% of the administered dose was excreted in the first 24 h. The urine was extracted, hydrolyzed, and fractionated. The bulk of the radioactive material (75%) was present in the phenolic sulfate fraction from which radiochemically pure equilin...
Wesson JA, Ginther OJ.Reproductive tracts were collected monthly over a 3-year period from 1,003 nonpregnant ponies at a slaughtering plant in Wisconsin. Ages of the animals were estimated from tooth replacement and wear. Ovaries were examined for follicular and luteal activity. There were no differences between left and right ovaries in size or occurrence of ovulation. The frequency of multiple ovulations, 10%, was higher than previously reported for ponies. The follicular changes during the ovulatory cycle did not support a two-wave theory of follicular growth. Ovarian activity changed seasonally, a finding simil...
Thompson DL, Pickett BW, Squires EL, Nett TM.Sixteen stallions were castrated and 30 days later assigned to one of four treatments: (1) testosterone propionate (175 microgram/kg body weight), (2) 17 beta-estradiol-3-benzoate (44 micrograms/kg body weight), (3) a combination of both steroids or, (4) vehicle only. These dosage were administered every other day for 18 days. The dosages were then doubled and continued for 20 days. Concentrations of testosterone and estradiol in serum decreased rapidly after castration and stabilized within about 6 hours. Mean concentrations of testosterone and estradiol maintained by the steroids were 1.4 an...
Bryans JT.Two herpesviruses produce diseases that affect reproductive efficiency in the horse. Equine herpesvirus 1 (EHV-1, equine rhinopneumonitis virus) is a promeinent cause of abortigenic infection as well as respiratory disease. Equine herpesvirus 3 (EHV-3, equine coital exanthema) is the cause of a benign progenital exanthema.
Bryans JT.The immunogenic potency and safety of a chemically inactivated equine herpesvirus 1 vaccine with added adjuvant was evaluated by testing serum-neutralizing and complement-fixation antibody responses of pregnant Thoroughbred mares. The vaccinated population comprised 321 pregnant mares on 7 farms; 3 in Normandy, France; 1 in Kildare, Ireland; and 3 in central Kentucky. The pattern of antibody response to vaccination was found qualitatively and quantitatively similar to that of pregnant mares previously vaccinated and determined by challenge exposure to be immune to abortigenic infection under e...
Trommershausen-Smith A.Dead or deformed foals produced by purebred parents represent an economic and emotional loss to the horse breeder. To avoid repeating production of such defective foals, the breeder may seek assistance in identifying the possible environmental or genetic causes for such animals. Only a few genetic diseases of the horse have been rigorously defined. Selected rare genetic diseases that prevent reproduction, cause the natural death or necessitate the humane destruction of a foal before it fulfills its intended purpose serve as examples for the definition of genetics of other deleterious diseases ...
Gunther JD, Foley CW, Gaverick HA, Plotka ED.Progesterone concentrations were measured in milk and blood plasma for 15 mares throughout a normal estrous cycle and early pregnancy to determine the feasibility of utilizing progesterone in milk as an indicator of pregnancy. Samples were obtained daily from foaling until diagnosis of pregnancy by rectal examination at 30 to 35 days of gestation. Progesterone in milk and blood plasma was quantified by radioimmunoassay. Mean progesterone concentrations (+/- SE) in plasma from foaling to foal heat and during estrus, luteal phase and pregnancy were .51 +/- .09 ng/ml, .53 +/- .08 ng/ml, 3.88 +/- ...
Bowling AT.Dead or deformed foals produced by purebred parents represent an economic and emotional loss to the horse breeder. In order to avoid producing such defective foals in the future, the breeder may seek guidance in determining whether their origin is environmental or genetic. Only a few genetic diseases of horses have been rigorously defined. Selected, rare genetic diseases that prevent reproduction, cause the natural death, or necessitate the humane destruction of a foal before it fulfills its intended purpose serve as examples for collecting evidence to define the genetics of other deleterious ...
Gherardi PB, Lindsay DR.Groups of 50 ewes were injected with serum from pregnant mares on 5 occasions, 3 months apart, and ovulation rate was estimated at laparoscopy. In Exp. 1, new ewes were used at each time and 3 dose rates (identical to 400, 630 and 1000 i.u. PMSG) were studied. In Exp. 2, the same ewes were used throughout and only 1 dose was given (1000 i.u.). In both experiments the response in ovulation rate of ewes varied significantly throughout the year, with the lowest response in spring (September) and the highest in autumn (March). The ewes in Exp. 2 were as responsive at the end as at the beginning of...
Hillman RB, Olar TT, Squires EL, Pickett BW.Stallion semen was collected, using artificial vaginas at 44 to 46, 48 to 50, and 52 to 54 C, to study the effects of temperature on seminal quality and sexual behavior. The temperature of the artificial vagina had no significant effect on motility, gel volume, gel-free seminal volume, total seminal volume, pH, number of mounts per ejaculate, total time to ejaculation, or seminal temperature. Spermatozoa were collected, then exposed to water-bath temperatures of 38, 45, 49, or 53 C for 1 minute. Mean motility was similar after exposure to temperatures of 38 or 45 C, but exposure to temperature...
Gleeson LJ, Coggins L.Twenty-one pregnant mares were inoculated with EHV1. Nineteen became infected as evidenced by clinical signs and/or viremia but only one mare aborted a virus-infected fetus. The viremias were leukocyte-associated and appeared to be non-productive, latent infections of these cells. Infectivity, detectable by cocultivation, persisted in the circulating leukocytes for as long as 9 days without resulting in abortion. The data suggest that it is extremely difficult to evaluate the efficacy of vaccines in preventing EHV1 (Rhinopneumonitis) abortion due to the paucity of non-exposed mares, lack of te...
Wingfield Digby NJ, Simons MA, Stockman MJ.Various type of insurance policies covering the risks against mortality, economic slaughter and loss of use, breeding failures and payment of veterinary fees are discussed. The role of the veterinary surgeon in supplying certificates of health and in relation to claims, including those involving destruction on humane grounds, are considered.
Pieńkowska-Schelling A, Handler J, Neuhauser S, Schelling C.The present case report describes a 6-year old subfertile pony mare, which became pregnant after the eleventh artificial insemination. The examination of the ovaries and the uterus did not reveal any abnormal clinical findings and the mare showed a regular oestrous cycle. Based on cytogenetic and molecular genetic analyses it became possible to elucidate the observed subfertility. The mosaic karyotype of the mare consisted of 63,X (20%) and 64,XX (80%) cells. A PCR analysis failed to amplify sequences from the equine SRY gene. The observed classic 63,X/64,XX mosaicism is a plausible explanatio...
Wissdorf H, Nautrup CP.The cavity, that belongs to the glans clitoridis and that has to be removed in accord with the regulations and rules for the import of horses of CEM carrier states, has not been taken up in the NAV (1983). Conform to the statements of the clinical physicians and to the declarations of the American literature the nomenclature "Sinus clitoridis" is proposed for the international use.
Kopp E, Mayr B, Schleger W.The expression of nucleolus organizer region (NOR) activity in diploid cells was investigated in a model mammalian hybrid system, the hinny (female ass x male horse), by sequential Ag-NOR and chromomycin A3/distamycin A/DAPI (CDD) staining ion lectin-stimulated peripheral blood lymphocytes. In the majority of cases we found non-expression of the horse-derived NOR chromosomes in the hinny. However, in one case there was strong NOR expression on horse-derived chromosome no. 1.
Nitschelm D, van der Horst CJ.It appeared that in mares suffering from endometritis the concentrations of pregnenolone, of the compound "5.4", and of progesterone in the blood during the first 2 days of the heat period were significantly higher than in normal cycling mares, in which the concentrations of all steroids were less than or equal to 1 ng/ml. In five endometritis mares the "5.4" and progesterone concentrations decreased after the first days of the heat period, and at about two days before ovulation the values were comparable to those of normal cycling mares. Eight mares suffering from a severe endometritis did no...
Nie GJ, Momont H.Three mares with regular estrous cycles and a large ovary were examined. In each case, the ovary was composed of a single, fluid-filled cavity with a thick capsule. The ovarian mass was surgically removed from each mare. Histologic diagnosis of each mass was different.
Botha AE, Schulman ML, Birrell J, du Plessis L, Laver PN, Soley J, Colenbrander B, Bertschinger HJ.The effects of the GnRH vaccine Improvac on testicular and epididymal morphometrics, histology and spermatogenesis were measured in 19 young (15-20 months) colts randomly assigned to one control (saline, castration at 57 days, n = 6) or either of two GnRH vaccine-treatment groups, T-57 (castration at 57 days, n = 7) or T-100 (castration at 100 days, n = 6), respectively. All were immunized on Day 0 with a single booster on Day 28. Excised testes and epididymides were weighed and processed for histology to measure tubule, epithelial and muscle dimensions, the ratio of interstitial tis...
Taylor NR.A case of balanoposthitis involving injury to a yearling colt's penis and prepuce is described. Details are given of the Vennerhalm or reefing operation which was used to relieve the penile prolapse and paraphimosis accompanying the condition.
Allen WE, Newcombe JR.The condition of anoestrus in the mare is described and the various causes of this syndrome are discussed. A regimen for examining systematically the clinically anoestrus mare is proposed, and methods of treatment are suggested.