Serology in horse research involves the study and analysis of blood serum to detect the presence of antibodies or antigens associated with infectious diseases and other health conditions. It is a diagnostic tool used to identify immune responses to pathogens, vaccination status, and exposure to specific diseases. Serological tests in equine research can include enzyme-linked immunosorbent assays (ELISA), complement fixation tests, and virus neutralization tests. These tests help in understanding the epidemiology of diseases, monitoring herd health, and informing vaccination strategies. This page compiles peer-reviewed research studies and scholarly articles that explore the methodologies, applications, and implications of serological testing in equine medicine.
Olitsky PK, Harford CG.Young (12 to 15 day old) mice are approximately as susceptible to the virus of equine encephalomyelitis, Eastern or Western strain, when it is given intraperitoneally as are adult mice when the virus is injected intracerebrally. With this susceptibility by the intraperitoneal route as a basis, the injection of immune serum-virus mixtures intraperitoneally was found to result in protection in dilutions which give rise to infection after intracerebral inoculation. The difference of protective power by the two indicated routes was shown not to depend on the amount of inoculum nor on the age of th...
Picanço EMB, Assis FFV, Peleja PL, Sousa ABB, Barrêto Júnior RA, Lima RF, Neves KAL, Minervino AHH.This study aimed to determine the prevalence of antibodies against equine brucellosis in three distinct equid groups (farm animal, urban carthorse, and sport horse) in Western Pará, Brazil, and to identify associated risk factors. A cross-sectional study, involving 75 farms across 14 municipalities, sampled 1069 equids composed of urban carthorses and sport horses. Serum samples were tested for antibodies against spp. using the buffered acidified plate antigen (BAPA) test for screening, followed by the serum agglutination in tubes with 2-mercaptoethanol (2-ME) for confirmation. Univariate an...
Gonzalez-Obando J, Jaimes-Dueñez J, Zuluaga-Cabrera A, Forero JE, Diaz A, Rojas-Arbeláez C, Ruiz-Saenz J.Equine influenza is a highly contagious disease caused by the equine influenza virus (EIV). The occurrence of EIV outbreaks in America is associated with low levels of vaccination coverage. In Colombia, no seroprevalence evaluation has been carried out to estimate the distribution of the virus within the country. Our aim was to perform a sero-epidemiological survey of equine influenza infections and to identify associated risk factors in horses from four departments of Colombia. Serological testing was carried out by using an ELISA for the detection of IgG antibodies against the influenza A vi...
Busquets N, Gardela J, José-Cunilleras E, Solé A, Salvador MJ, Obón E, Molina-López R, Aranda C, Montalvo T, Corbella I, Bou-Monclús MA....West Nile virus (WNV), mainly transmitted by mosquitoes, poses significant health risks to humans and horses, particularly in endemic regions. The first detection of WNV lineage 2 in Spain was in 2017 in Catalonia (northeastern Spain). In 2023, WNV was confirmed in a young yellow-legged gull and a probable human case was notified within the urban settings. We aimed to define the zone of WNV circulation in the Barcelona Metropolitan Area where these infections occurred and the effectiveness of the One Health approach for early WNV detection. The Catalan WNV surveillance and control programs in...
Schwarzer A, Ziegler U, Fertey J, Kreuz M, Vahlenkamp TW, Groschup MH, Ulbert S.West Nile virus (WNV), Usutu virus (USUV) and tick-borne encephalitis virus (TBEV) are worldwide endemic zoonotic orthoflaviviruses, often co-circulating in the same areas. Serological studies in animals, mostly birds and horses, are important means to monitor the spread of these viruses and the infection risks for humans. However, cross-reactive antibodies to these structurally similar flaviviruses frequently impact serological differentiation in enzyme-linked immunosorbent assays (ELISAs), hence time-consuming virus neutralization tests (VNTs) have to be employed in laboratories with high bi...
Ganbaatar O, Batbaatar V, Kimura T, Ganzorig S, Tseren-Ochir EO, Suzuki Y, Takai S.ELISA antibody titers against Rhodococcus equi strain ATCC 6939 were measured in serum samples collected from 644 horses aged 1-21 years at 13 provinces and the capital of Mongolia, of which 631 (98%) samples showed values of approximately zero. The mean antibody titers in the 14 areas ranged from -0.08 to -0.01. Nine samples (1.4%) exhibited titers of 0.1-0.2, and surprisingly, four horses (0.6%) each aged 2, 5, 8, and 9 years demonstrated positive values in the range of 0.3-0.8. Considering the age resistance characteristic of this disease, the positive horses were hypothesized to exhibit no...
