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Veterinary surgery : VS2012; 41(7); 784-794; doi: 10.1111/j.1532-950X.2012.01016.x

Activation of equine platelet-rich plasma: comparison of methods and characterization of equine autologous thrombin.

Abstract: To investigate and compare clinically relevant Platelet-rich plasma (PRP) activation methods. Methods: Experimental. Methods: PRP was prepared from 6 equine subjects. Activation of the PRP was performed by 4 methods (autologous thrombin, bovine thrombin, calcium chloride (CaCl(2) ), or freeze-thaw). The resultant PDGF-BB (where PDGF is platelet-derived growth factor) and TGFβ1 (where TGFβ is transforming growth factor beta) levels in PRP releasates were quantified by Enzyme-linked immunosorbent assay (ELISA) and compared. Growth factor contents were also compared between platelet-rich clots produced by thrombin or CaCl(2) . The composition and function of equine autologous thrombin were characterized by Western blot analysis and platelet aggregometry. Results: CaCl(2) (23 mM) activation of PRP yielded significantly greater PDGF release than did any other method. TGFβ release was comparable after PRP activation by CaCl(2) , bovine thrombin, and freeze thaw. Autologous thrombin was significantly less effective than all other activation methods in eliciting platelet growth factor release and induced significantly less platelet aggregation than bovine thrombin at 5 U/mL. Clots retained substantial concentrations of growth factor, and the amount in the releasate versus the clot differed between activation methods. Conclusions: PRP activation methods differ in terms of growth factor output as well as logistical considerations. Autologous thrombin is not recommended for PRP activation. CaCl(2) (23 mM) is an effective and inexpensive method of PRP activation. The PRP releasate derived from CaCl(2) activation contains 80% of the total PDGF content and is easily produced, making it a convenient product for clinical use.
© Copyright 2012 by The American College of Veterinary Surgeons.
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Publication Date: 2012-06-28 PubMed ID: 22742830DOI: 10.1111/j.1532-950X.2012.01016.xGoogle Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research compares different methods of activating Platelet-rich plasma (PRP) in horses. The study discovered that the use of calcium chloride (CaCl(2)) yields significantly greater platelet-derived growth factor (PDGF) release compared to other methods, and autologous thrombin is not recommended for PRP activation.

Study Design and Methods

  • The researchers conducted this experimental study on six equine subjects. They prepared Platelet-rich plasma (PRP) from each of the subjects.
  • The team used four specific methods to activate the PRP: autologous thrombin, bovine thrombin, calcium chloride (CaCl(2)), and freeze-thaw.
  • The levels of PDGF-BB, a type of platelet-derived growth factor, and TGFβ1, a transforming growth factor beta, in the PRP were quantified using ELISA.
  • Growth factor contents were compared between the platelet-rich clots produced using thrombin and those created with CaCl(2).
  • The study evaluated the composition and function of the equine autologous thrombin using Western blot analysis and platelet aggregometry.

Results of the Study

  • The use of calcium chloride(CaCl(2)) for PRP activation resulted in the greatest release of PDGF compared to other methods.
  • TGFβ release was comparable after PRP activation by CaCl(2), bovine thrombin, and freeze thaw.
  • Autologous thrombin was found to be significantly less effective in inducing platelet growth factor release than all other methods.
  • Autologous thrombin also induced significantly less platelet aggregation than bovine thrombin at 5 U/mL.
  • The clots maintained significant concentrations of growth factor, but the amount released versus the clot depended on the activation method used.

Conclusions and Recommendations

  • The researchers concluded that the different methods of PRP activation vary in terms of growth factor output and logistical considerations.
  • They specifically advised against the use of autologous thrombin for PRP activation.
  • They found that calcium chloride (CaCl(2)), specifically at a concentration of 23 mM, is a cost-effective method for PRP activation.
  • The team identified that the PRP releasate obtained from CaCl(2) activation contained approximately 80% of the total PDGF content and is easily produced, making it a suitable product for clinical application.

Cite This Article

APA
Textor JA, Tablin F. (2012). Activation of equine platelet-rich plasma: comparison of methods and characterization of equine autologous thrombin. Vet Surg, 41(7), 784-794. https://doi.org/10.1111/j.1532-950X.2012.01016.x

Publication

Veterinary surgery : VS
ISSN: 1532-950X
NlmUniqueID: 8113214
Country: United States
Language: English
Volume: 41
Issue: 7
Pages: 784-794

Researcher Affiliations

Textor, Jamie A
  • Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California-Davis, Davis, CA 95616, USA. jamietextor@gmail.com
Tablin, Fern

    MeSH Terms

    • Animals
    • Blood Coagulation / physiology
    • Blood Transfusion, Autologous / veterinary
    • Cattle
    • Female
    • Horses / blood
    • Male
    • Platelet Activation / physiology
    • Platelet-Derived Growth Factor / metabolism
    • Platelet-Rich Plasma / cytology
    • Platelet-Rich Plasma / physiology
    • Thrombin / chemistry
    • Thrombin / classification
    • Thrombin / metabolism
    • Time Factors

    Citations

    This article has been cited 41 times.
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