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Home / Equine Research Bank / Parasite Epidemiol Control / Anthelmintics efficacy against intestinal strongyles in hors...
Parasite epidemiology and control2016; 1(2); 15-19; doi: 10.1016/j.parepi.2016.01.001

Anthelmintics efficacy against intestinal strongyles in horses of Sardinia, Italy.

Abstract: Intestinal strongyles (IS) are the most important parasites of equids, due to their high prevalence worldwide, pathogenicity and the spread of drug-resistant populations. Despite the large number of horses bred in Sardinia Island, Italy, no data are available on the efficacy of anthelmintic compounds in the control of horse strongylosis. Therefore the aim of the present study was to evaluate the efficacy of five commercial anthelmintic formulations containing fenbendazole (FBZ), pyrantel (PYR), moxidectin (MOX) and two ivermectin formulations (IVM1 and IVM2) against IS in Sardinia by performing a fecal egg count reduction test (FECRT) and investigating the egg reappearance period (ERP) after treatment. In total, 74 horses from 7 farms were examined. Coprocultures performed for individual fecal samples collected at the day of the treatment revealed that cyathostomins were the predominant parasitic species (98.6%). The FECR for all horses belonging to the treatment groups after two weeks was ≥ 95% with a 95% C.I. > 90%. The expected ERP did not decrease in any of the treatment group as FECR values  90% for the entire duration of the trial until D150. The results of the present survey indicate that drug-resistant cyathostomin populations are not present in the examined horse population, contrariwise to what observed in other Italian and European regions. The reasons and implications of these results are discussed.
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Publication Date: 2016-03-08 PubMed ID: 29988184PubMed Central: PMC5991823DOI: 10.1016/j.parepi.2016.01.001Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article studies the effectiveness of five different anti-parasitic drugs on intestinal strongyles (a type of parasitic worm) in horses on the island of Sardinia, Italy. Through fecal examination, the researchers determined the presence and amount of worm infection, and evaluated the duration of each drug’s effectiveness.

Study Objectives and Methods

  • The research aimed to assess the effectiveness of five commercial anti-parasitic drugs (fenbendazole, pyrantel, moxidectin, and two formulations of ivermectin) against intestinal strongyles in horses. Intestinal strongyles are a type of worm and a major concern for equines due to their wide prevalence and the increasing instances of drug-resistant strains.
  • The study also sought to investigate the egg reappearance period (ERP), the interval after drug administration before eggs can be detected in the feces again, indicating a continuing or reintroduced parasitic infection.
  • To achieve these objectives, the researchers performed a fecal egg count reduction test (FECRT) at different time points after treatment. The study involved 74 horses from 7 different farms in Sardinia, Italy.

Main Findings

  • The predominant parasitic species found in the sample horses was cyathostomins, accounting for about 98.6% of the parasites detected.
  • All drug treatments led to a powerful response, reducing the fecal egg count by at least 95% after just two weeks, and indicating an effective immediate response against the parasites.
  • The research also found differences in the egg reappearance period (ERP) between the drugs tested. For fenbendazole, eggs reappeared in the feces at day 60 after treatment. For pyrantel and one formulation of ivermectin (IVM1), eggs reappeared at day 90, and for the second ivermectin formulation (IVM2), at day 150. For moxidectin, the fecal egg count remained reduced by at least 90% until day 150, suggesting it had the most extended efficacy.

Implications and Significance

  • The research indicates that in the studied horse population on Sardinia, there is not yet a significant problem with drug-resistant cyathostomin parasite populations. This differs from the situation reported in other Italian and European regions, suggesting regional or management factors impacting drug resistance rates.
  • The results can be used by equine health professionals in Sardinia to guide selection of parasite treatment strategies, contributing to maintaining drug efficacy and slowing the development of resistant parasites.
  • The variability seen in drug effectiveness could befall due to several factors, including differences in parasitic life cycles, drug pharmacokinetics, or exposure to environmental contamination. Understanding and optimizing these factors can aid in designing targeted and effective parasite control programs for horses.

Cite This Article

APA
Sanna G, Pipia AP, Tamponi C, Manca R, Varcasia A, Traversa D, Scala A. (2016). Anthelmintics efficacy against intestinal strongyles in horses of Sardinia, Italy. Parasite Epidemiol Control, 1(2), 15-19. https://doi.org/10.1016/j.parepi.2016.01.001

Publication

Parasite epidemiology and control
ISSN: 2405-6731
NlmUniqueID: 101687137
Country: Netherlands
Language: English
Volume: 1
Issue: 2
Pages: 15-19

Researcher Affiliations

Sanna, G
  • Dipartimento di Medicina Veterinaria, Settore di Parassitologia e Malattie Parassitarie, Università degli Studi di Sassari, Via Vienna 2, 07100 Sassari, Italy.
Pipia, A P
  • Dipartimento di Medicina Veterinaria, Settore di Parassitologia e Malattie Parassitarie, Università degli Studi di Sassari, Via Vienna 2, 07100 Sassari, Italy.
Tamponi, C
  • Dipartimento di Medicina Veterinaria, Settore di Parassitologia e Malattie Parassitarie, Università degli Studi di Sassari, Via Vienna 2, 07100 Sassari, Italy.
Manca, R
  • Veterinary practitioner, Sassari, Italy.
Varcasia, A
  • Dipartimento di Medicina Veterinaria, Settore di Parassitologia e Malattie Parassitarie, Università degli Studi di Sassari, Via Vienna 2, 07100 Sassari, Italy.
Traversa, D
  • Facoltà di Medicina Veterinaria, Università degli Studi di Teramo, Piazza A. Moro 45, 64100 Teramo, Italy.
Scala, A
  • Dipartimento di Medicina Veterinaria, Settore di Parassitologia e Malattie Parassitarie, Università degli Studi di Sassari, Via Vienna 2, 07100 Sassari, Italy.

References

This article includes 25 references
  1. Coles GC, Bauer C, Borgsteede FH, Geerts S, Klei TR, Taylor MA, Waller PJ. World Association for the Advancement of Veterinary Parasitology (W.A.A.V.P.) methods for the detection of anthelmintic resistance in nematodes of veterinary importance.. Vet Parasitol 1992 Sep;44(1-2):35-44.
    pubmed: 1441190doi: 10.1016/0304-4017(92)90141-ugoogle scholar: lookup
  2. Genchi C, Di Sacco B, Traldi G, Bogara B, Quintavalla F. Prime osservazioni in Italia sulla resistenza dei piccoli strongili del cavallo (Cyathostominae) ai benzimidazolici ed efficacia del pyrantel pamoato. Ippologia 1992;3(2):77–88.
  3. Geurden T, van Doorn D, Claerebout E, Kooyman F, De Keersmaecker S, Vercruysse J, Besognet B, Vanimisetti B, di Regalbono AF, Beraldo P, Di Cesare A, Traversa D. Decreased strongyle egg re-appearance period after treatment with ivermectin and moxidectin in horses in Belgium, Italy and The Netherlands.. Vet Parasitol 2014 Aug 29;204(3-4):291-6.
    pubmed: 24880643doi: 10.1016/j.vetpar.2014.04.013google scholar: lookup
  4. Heidi A.B., Wade T.N.. Drug resistance in equine parasites: an emerging global problem. J. Equine Vet. Sci. 2009;29(5):285–295.
  5. Kaplan R.M., Nielsen M.K.. An evidence-based approach to equine parasite control: it ain't the 60s anymore. Equine Vet. Educ. 2010;22:306–316.
  6. Larsen ML, Ritz C, Petersen SL, Nielsen MK. Determination of ivermectin efficacy against cyathostomins and Parascaris equorum on horse farms using selective therapy.. Vet J 2011 Apr;188(1):44-7.
    pubmed: 20385508doi: 10.1016/j.tvjl.2010.03.009google scholar: lookup
  7. Lyons ET, Tolliver SC, Collins SS. Reduced activity of moxidectin and ivermectin on small strongyles in young horses on a farm (BC) in Central Kentucky in two field tests with notes on variable counts of eggs per gram of feces (EPGs).. Parasitol Res 2011 May;108(5):1315-9.
    pubmed: 21212983doi: 10.1007/s00436-010-2225-zgoogle scholar: lookup
  8. . Manual of veterinary parasitological techniques. MAFF (Ministry of Agriculture Fisheries and Food) Technical Bulletin No.18. third ed. Her Majesty's Stationery Office; London, UK: 1986; pp. 39–40.
  9. Molento MB, Nielsen MK, Kaplan RM. Resistance to avermectin/milbemycin anthelmintics in equine cyathostomins - current situation.. Vet Parasitol 2012 Apr 19;185(1):16-24.
    pubmed: 22047763doi: 10.1016/j.vetpar.2011.10.013google scholar: lookup
  10. Montinaro S., Scala A., Battelli G., Stancampiano L.. Epidemiologia delle infestioni gastrointestinali del cavallo. Emissione di uova di nematodi nella popolazione equina sarda. ODV 2002;23:35–42.
  11. Nielsen MK, Reinemeyer CR, Donecker JM, Leathwick DM, Marchiondo AA, Kaplan RM. Anthelmintic resistance in equine parasites--current evidence and knowledge gaps.. Vet Parasitol 2014 Jul 30;204(1-2):55-63.
    pubmed: 24433852doi: 10.1016/j.vetpar.2013.11.030google scholar: lookup
  12. Papini R.A., Micol De Bernart F., Sgorbini M.. A questionnaire survey on intestinal worm control practices in horses in Italy. J. Equine Vet. Sci. 2015;35:70–75.
  13. Peregrine AS, Molento MB, Kaplan RM, Nielsen MK. Anthelmintic resistance in important parasites of horses: does it really matter?. Vet Parasitol 2014 Mar 17;201(1-2):1-8.
    pubmed: 24485565doi: 10.1016/j.vetpar.2014.01.004google scholar: lookup
  14. Piergili-Fioretti D., Veronesi F., Diaferia M., Pepe M.. Epidemiologia delle infestazioni equine da Anoplocephalidae e Cyathostominae in Umbria. ODV 2005;2:25–34.
  15. Pilo C, Altea A, Pirino S, Nicolussi P, Varcasia A, Genchi M, Scala A. Strongylus vulgaris (Looss, 1900) in horses in Italy: is it still a problem?. Vet Parasitol 2012 Mar 23;184(2-4):161-7.
    pubmed: 21962966doi: 10.1016/j.vetpar.2011.09.016google scholar: lookup
  16. Raynaud JP. [Study of the efficiency of a quantitative coproscopic technic for the routine diagnosis and control of parasitic infestations of cattle, sheep, horses and swine].. Ann Parasitol Hum Comp 1970 May-Jun;45(3):321-42.
    pubmed: 5531507
  17. Roberts F.H.S., O'Sullivan P.J.. Methods for egg count and larval cultures for strongyle infesting the gastro-intestinal tract of cattle. Aust. Agric. Res. 1950;1:99–102.
  18. Sallé G, Cabaret J. A survey on parasite management by equine veterinarians highlights the need for a regulation change.. Vet Rec Open 2015;2(2):e000104.
    pmc: PMC4581345pubmed: 26421153doi: 10.1136/vetreco-2014-000104google scholar: lookup
  19. Sangster NC. Pharmacology of anthelmintic resistance in cyathostomes: will it occur with the avermectin/milbemycins?. Vet Parasitol 1999 Aug 31;85(2-3):189-201; discussion 201-4, 215-25.
    pubmed: 10485365doi: 10.1016/s0304-4017(99)00099-0google scholar: lookup
  20. Stratford CH, McGorum BC, Pickles KJ, Matthews JB. An update on cyathostomins: anthelmintic resistance and diagnostic tools.. Equine Vet J Suppl 2011 Aug;(39):133-9.
    pubmed: 21790768doi: 10.1111/j.2042-3306.2011.00397.xgoogle scholar: lookup
  21. Traldi G., Manfredi M.T., Rosa M.. Le strongilosi intestinali dell'equino. Praxis Vet. 1988;9:11–13.
  22. Traversa D, Klei TR, Iorio R, Paoletti B, Lia RP, Otranto D, Sparagano OA, Giangaspero A. Occurrence of anthelmintic resistant equine cyathostome populations in central and southern Italy.. Prev Vet Med 2007 Dec 14;82(3-4):314-20.
    pubmed: 17719664doi: 10.1016/j.prevetmed.2007.07.006google scholar: lookup
  23. Traversa D, von Samson-Himmelstjerna G, Demeler J, Milillo P, Schürmann S, Barnes H, Otranto D, Perrucci S, di Regalbono AF, Beraldo P, Boeckh A, Cobb R. Anthelmintic resistance in cyathostomin populations from horse yards in Italy, United Kingdom and Germany.. Parasit Vectors 2009 Sep 25;2 Suppl 2(Suppl 2):S2.
    pmc: PMC2751838pubmed: 19778463doi: 10.1186/1756-3305-2-s2-s2google scholar: lookup
  24. Traversa D, Castagna G, von Samson-Himmelstjerna G, Meloni S, Bartolini R, Geurden T, Pearce MC, Woringer E, Besognet B, Milillo P, D'Espois M. Efficacy of major anthelmintics against horse cyathostomins in France.. Vet Parasitol 2012 Sep 10;188(3-4):294-300.
    pubmed: 22538094doi: 10.1016/j.vetpar.2012.03.048google scholar: lookup
  25. von Samson-Himmelstjerna G. Anthelmintic resistance in equine parasites - detection, potential clinical relevance and implications for control.. Vet Parasitol 2012 Apr 19;185(1):2-8.
    pubmed: 22100141doi: 10.1016/j.vetpar.2011.10.010google scholar: lookup

Citations

This article has been cited 3 times.
  1. Macdonald SL, Abbas G, Ghafar A, Gauci CG, Bauquier J, El-Hage C, Tennent-Brown B, Wilkes EJA, Beasley A, Jacobson C, Cudmore L, Carrigan P, Hurley J, Beveridge I, Hughes KJ, Nielsen MK, Jabbar A. Egg reappearance periods of anthelmintics against equine cyathostomins: The state of play revisited. Int J Parasitol Drugs Drug Resist 2023 Apr;21:28-39.
    doi: 10.1016/j.ijpddr.2022.12.002pubmed: 36543048google scholar: lookup
  2. Scala A, Tamponi C, Sanna G, Predieri G, Dessì G, Sedda G, Buono F, Cappai MG, Veneziano V, Varcasia A. Gastrointestinal Strongyles Egg Excretion in Relation to Age, Gender, and Management of Horses in Italy. Animals (Basel) 2020 Dec 3;10(12).
    doi: 10.3390/ani10122283pubmed: 33287298google scholar: lookup
  3. Nielsen MK. Anthelmintic resistance in equine nematodes: Current status and emerging trends. Int J Parasitol Drugs Drug Resist 2022 Dec;20:76-88.
    doi: 10.1016/j.ijpddr.2022.10.005pubmed: 36342004google scholar: lookup
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