Analyze Diet
Animal : an international journal of animal bioscience2012; 6(2); 227-231; doi: 10.1017/S1751731111001297

Apparent digestibility of wheat bran and extruded flax in horses determined from the total collection of feces and acid-insoluble ash as an internal marker.

Abstract: Several studies have reported data on comparisons between two methods: the total collection of feces and the internal markers method. The aim of this study was to assess the apparent digestibility of two concentrates and to compare the apparent digestion coefficients using the total collection of feces and acid-insoluble ash (AIA) as the internal marker method. In 2009, six adult geldings aged between 3 and 11 years, with an average weight per trial of 543, 540 and 542 kg, respectively, were used to determine the apparent digestibility by means of three in vivo digestibility trials on hay, hay plus wheat bran (60 : 40) and hay plus extruded flax (80 : 20). Feces were collected over a 6-day period with a previous 14-day adaptation period. The three digestibility trials were carried out to determine the digestion coefficients of the three diets and, indirectly, of the two concentrates. The digestion coefficients of the diets were determined for the dry matter, organic matter, crude protein and gross energy, whereas the apparent digestion coefficients of the same parameters were calculated for wheat bran and extruded flax, by calculating the difference from the previous results. The data were analyzed using the Student t-test for paired samples. The digestion coefficients obtained were similar when the total collection of feces and the AIA method were used. Higher data variability, confirmed by a greater standard deviation, was observed using the AIA method to estimate the apparent digestion coefficients. It can be concluded that the use of AIA as an internal marker in digestibility trials on average leads to values similar to those obtained with the total collection of feces and can therefore be considered a less-expensive method to determine apparent digestion coefficients. Nevertheless, the total collection of feces should still be considered the best choice to determine the digestibility of some specific feedstuffs.
Publication Date: 2012-03-23 PubMed ID: 22436180DOI: 10.1017/S1751731111001297Google Scholar: Lookup
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  • Comparative Study
  • Journal Article

Summary

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The research article investigates the digestibility of wheat bran and extruded flax in horses, comparing methods of using total feces collection and acid-insoluble ash (AIA) as an internal marker. The goal is to determine accurate digestion coefficients for these feeds, identifying whether the AIA method can provide a less expensive but equally accurate alternative to total feces collection.

Study Design and Procedure

  • In this research, scientists conducted trials on six adult geldings (castrated male horses) ranging from 3 to 11 years old, each averaging around 540 kilograms in weight during the trials.
  • The horses were subjected to three in vivo digestibility trials on varying diets; hay only, a combination of hay and wheat bran (60:40), and a combination of hay and extruded flax (80:20).
  • A 14-day adaptation period was held prior to each six-day feces collection stretch, where diet digestibility was determined.
  • Digestion coefficients of the three diets were measured for dry matter, organic matter, crude protein, and gross energy. Apparent digestion coefficients for wheat bran and extruded flax were also calculated by determining the difference from the primary digestion coefficient results.
  • Data analysis was done using a Student t-test for paired samples.

Results and Conclusions

  • The research found similar digestion coefficients when using the total collection of feces and the AIA method, indicating both strategies have comparable accuracy.
  • AIA method exhibited a higher data variability, as indicated by a larger standard deviation, in estimating apparent digestion coefficients.
  • While the study concludes that AIA can be a less expensive method for determining apparent digestion coefficients, it still upholds the total collection of feces as the best choice for determining the digestibility of specific feeds due to its higher data stability.

This study contributes to better understanding of determining feed digestion in horses, which is crucial for optimizing their diet for better health and performance. Its findings can be used to inform feed practices and further research in equine nutrition and digestive physiology.

Cite This Article

APA
De Marco M, Miraglia N, Peiretti PG, Bergero D. (2012). Apparent digestibility of wheat bran and extruded flax in horses determined from the total collection of feces and acid-insoluble ash as an internal marker. Animal, 6(2), 227-231. https://doi.org/10.1017/S1751731111001297

Publication

ISSN: 1751-732X
NlmUniqueID: 101303270
Country: England
Language: English
Volume: 6
Issue: 2
Pages: 227-231

Researcher Affiliations

De Marco, M
  • DIPAEE, Torino University, Via L. Da Vinci 44, 10095 Grugliasco (TO), Italy. domenico.bergero@unito.it
Miraglia, N
    Peiretti, P G
      Bergero, D

        MeSH Terms

        • Animals
        • Carbon / metabolism
        • Dietary Fiber / metabolism
        • Digestion / physiology
        • Feces / chemistry
        • Flax / metabolism
        • Horses / physiology
        • Nutritive Value
        • Poaceae / metabolism

        Citations

        This article has been cited 6 times.
        1. Fehlberg LK, Lattimer JM, Vahl CI, Drouillard JS, Douthit TL. Digestibility of diets containing calcium salts of fatty acids or soybean oil in horses. Transl Anim Sci 2020 Apr;4(2):txaa001.
          doi: 10.1093/tas/txaa001pubmed: 32705004google scholar: lookup
        2. Saastamoinen M, Särkijärvi S. Effect of Linseed (Linum usitatissimum) Groats-Based Mixed Feed Supplements on Diet Nutrient Digestibility and Blood Parameters of Horses. Animals (Basel) 2020 Feb 10;10(2).
          doi: 10.3390/ani10020272pubmed: 32050686google scholar: lookup
        3. Dong L, Li B, Diao Q. Effects of Dietary Forage Proportion on Feed Intake, Growth Performance, Nutrient Digestibility, and Enteric Methane Emissions of Holstein Heifers at Various Growth Stages. Animals (Basel) 2019 Sep 26;9(10).
          doi: 10.3390/ani9100725pubmed: 31561414google scholar: lookup
        4. Alvarenga IC, Aldrich CG, Ou Z. Comparison of four digestibility markers to estimate fecal output of dogs. J Anim Sci 2019 Mar 1;97(3):1036-1041.
          doi: 10.1093/jas/skz020pubmed: 30753619google scholar: lookup
        5. Alvarenga IC, Aldrich CG, Kohles M. The Effect of Feed Form on Diet Digestibility and Cecal Parameters in Rabbits. Animals (Basel) 2017 Dec 7;7(12).
          doi: 10.3390/ani7120095pubmed: 29215569google scholar: lookup
        6. Kohl KD, Barts N, Peralta Martínez K, Lackey A, Lyons E, Maier MJ, Maurer M, Tripoli D, Yawitz T, Martínez-Mota R, Pasch B, Dearing MD, Trevelline BK. How consistent is 'the dynamic gut'? Complex physiological responses to dietary fiber and protein across three rodent species. J Exp Biol 2025 Jul 15;228(14).
          doi: 10.1242/jeb.249797pubmed: 40351100google scholar: lookup