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Home / Equine Research Bank / Nat Commun / Binding and structural basis of equine ACE2 to RBDs from SAR...
Nature communications2022; 13(1); 3547; doi: 10.1038/s41467-022-31276-6

Binding and structural basis of equine ACE2 to RBDs from SARS-CoV, SARS-CoV-2 and related coronaviruses.

Abstract: The origin and host range of SARS-CoV-2, the causative agent of coronavirus disease 2019 (COVID-19), are important scientific questions as they might provide insight into understanding of the potential future spillover to infect humans. Here, we tested the binding between equine angiotensin converting enzyme 2 (eqACE2) and the receptor binding domains (RBDs) of SARS-CoV, SARS-CoV-2 prototype (PT) and variant of concerns (VOCs), as well as their close relatives bat-origin coronavirus (CoV) RaTG13 and pangolin-origin CoVs GX/P2V/2017 and GD/1/2019. We also determined the crystal structures of eqACE2/RaTG13-RBD, eqACE2/SARS-CoV-2 PT-RBD and eqACE2/Omicron BA.1-RBD. We identified S494 of SARS-COV-2 PT-RBD as an important residue in the eqACE2/SARS-COV-2 PT-RBD interaction and found that N501Y, the commonly recognized enhancing mutation, attenuated the binding affinity with eqACE2. Our work demonstrates that horses are potential targets for SARS-CoV-2 and highlights the importance of continuous surveillance on SARS-CoV-2 and related CoVs to prevent spillover events.
© 2022. The Author(s).
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Publication Date: 2022-06-21 PubMed ID: 35729237PubMed Central: PMC9210341DOI: 10.1038/s41467-022-31276-6Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research looked at the interaction between various coronavirus strains and the equine angiotensin converting enzyme 2 (eqACE2), determining that horses might be potential targets for these viruses including SARS-CoV-2.

Research Focus

  • The aim of this research was to better understand the host range and origin of SARS-CoV-2, along with other related coronaviruses. This is crucial to gain insight into potential future spillover scenarios where these viruses can infect humans.

Methodology

  • The researchers tested the bindings between eqACE2, which is a receptor found in horses, and the receptor binding domains (RBDs) of various versions of the severe acute respiratory syndrome coronaviruses SARS-CoV and SARS-Cov-2, including the prototype and variants of concern (VOCs).
  • They also tested bindings with RBDs found in other coronaviruses that originate from bats and pangolins.
  • The research team also used crystallography to determine the structural elements of these bindings.

Findings

  • Through these tests, the research team pinpointed the S494 on the SARS-CoV2 PT-RBD as a crucial residue that interacts with eqACE2.
  • Interestingly, they also found that the mutation N501Y, which is widely recognized as enhancing the virus’ ability to bind with human cells, actually diminished the binding affinity with eqACE2.

Conclusions

  • The findings demonstrate that horses could be potential hosts for SARS-CoV-2 and other related coronaviruses.
  • This kind of research is crucial for ongoing surveillance of these viruses to prevent future spillover events that could potentially lead to outbreaks in the human population.

Cite This Article

APA
Xu Z, Kang X, Han P, Du P, Li L, Zheng A, Deng C, Qi J, Zhao X, Wang Q, Liu K, Gao GF. (2022). Binding and structural basis of equine ACE2 to RBDs from SARS-CoV, SARS-CoV-2 and related coronaviruses. Nat Commun, 13(1), 3547. https://doi.org/10.1038/s41467-022-31276-6

Publication

Nature communications
ISSN: 2041-1723
NlmUniqueID: 101528555
Country: England
Language: English
Volume: 13
Issue: 1
Pages: 3547
PII: 3547

Researcher Affiliations

Xu, Zepeng
  • CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
  • Faculty of Health Sciences, University of Macau, Macau SAR, 999078, China.
Kang, Xinrui
  • CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
  • Savaid Medical School, University of Chinese Academy of Sciences, Beijing, 100049, China.
Han, Pu
  • CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
Du, Pei
  • CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
Li, Linjie
  • CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
  • University of Chinese Academy of Sciences, Beijing, 100049, China.
Zheng, Anqi
  • CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
  • University of Chinese Academy of Sciences, Beijing, 100049, China.
Deng, Chuxia
  • Faculty of Health Sciences, University of Macau, Macau SAR, 999078, China.
Qi, Jianxun
  • CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
  • University of Chinese Academy of Sciences, Beijing, 100049, China.
Zhao, Xin
  • CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
Wang, Qihui
  • CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China. wangqihui@im.ac.cn.
  • University of Chinese Academy of Sciences, Beijing, 100049, China. wangqihui@im.ac.cn.
Liu, Kefang
  • CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China. Liukf@im.ac.cn.
Gao, George Fu
  • CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

MeSH Terms

  • Angiotensin-Converting Enzyme 2
  • Animals
  • COVID-19
  • Horses
  • Peptidyl-Dipeptidase A / metabolism
  • Protein Binding
  • SARS-CoV-2 / genetics
  • Spike Glycoprotein, Coronavirus / metabolism

Conflict of Interest Statement

The authors declare no competing interests.

References

This article includes 57 references
  1. Tan W, Zhao X, Ma X, Wang W, Niu P, Xu W, Gao GF, Wu G. A Novel Coronavirus Genome Identified in a Cluster of Pneumonia Cases - Wuhan, China 2019-2020.. China CDC Wkly 2020 Jan 24;2(4):61-62.
    doi: 10.46234/ccdcw2020.017pmc: PMC8393069pubmed: 34594763google scholar: lookup
  2. Gao GF, Wang L. COVID-19 Expands Its Territories from Humans to Animals.. China CDC Wkly 2021 Oct 8;3(41):855-858.
    doi: 10.46234/ccdcw2021.210pmc: PMC8521158pubmed: 34703641google scholar: lookup
  3. Zhang Q, Zhang H, Gao J, Huang K, Yang Y, Hui X, He X, Li C, Gong W, Zhang Y, Zhao Y, Peng C, Gao X, Chen H, Zou Z, Shi ZL, Jin M. A serological survey of SARS-CoV-2 in cat in Wuhan.. Emerg Microbes Infect 2020 Dec;9(1):2013-2019.
    doi: 10.1080/22221751.2020.1817796pmc: PMC7534315pubmed: 32867625google scholar: lookup
  4. Patterson EI, Elia G, Grassi A, Giordano A, Desario C, Medardo M, Smith SL, Anderson ER, Prince T, Patterson GT, Lorusso E, Lucente MS, Lanave G, Lauzi S, Bonfanti U, Stranieri A, Martella V, Solari Basano F, Barrs VR, Radford AD, Agrimi U, Hughes GL, Paltrinieri S, Decaro N. Evidence of exposure to SARS-CoV-2 in cats and dogs from households in Italy.. Nat Commun 2020 Dec 4;11(1):6231.
    doi: 10.1038/s41467-020-20097-0pmc: PMC7718263pubmed: 33277505google scholar: lookup
  5. Sit THC, Brackman CJ, Ip SM, Tam KWS, Law PYT, To EMW, Yu VYT, Sims LD, Tsang DNC, Chu DKW, Perera RAPM, Poon LLM, Peiris M. Infection of dogs with SARS-CoV-2.. Nature 2020 Oct;586(7831):776-778.
    doi: 10.1038/s41586-020-2334-5pmc: PMC7606701pubmed: 32408337google scholar: lookup
  6. Barrs VR, Peiris M, Tam KWS, Law PYT, Brackman CJ, To EMW, Yu VYT, Chu DKW, Perera RAPM, Sit THC. SARS-CoV-2 in Quarantined Domestic Cats from COVID-19 Households or Close Contacts, Hong Kong, China.. Emerg Infect Dis 2020 Dec;26(12):3071-3074.
    doi: 10.3201/eid2612.202786pmc: PMC7706951pubmed: 32938527google scholar: lookup
  7. Oreshkova N, Molenaar RJ, Vreman S, Harders F, Oude Munnink BB, Hakze-van der Honing RW, Gerhards N, Tolsma P, Bouwstra R, Sikkema RS, Tacken MG, de Rooij MM, Weesendorp E, Engelsma MY, Bruschke CJ, Smit LA, Koopmans M, van der Poel WH, Stegeman A. SARS-CoV-2 infection in farmed minks, the Netherlands, April and May 2020.. Euro Surveill 2020 Jun;25(23).
    doi: 10.2807/1560-7917.ES.2020.25.23.2001005pmc: PMC7403642pubmed: 32553059google scholar: lookup
  8. Oude Munnink BB, Sikkema RS, Nieuwenhuijse DF, Molenaar RJ, Munger E, Molenkamp R, van der Spek A, Tolsma P, Rietveld A, Brouwer M, Bouwmeester-Vincken N, Harders F, Hakze-van der Honing R, Wegdam-Blans MCA, Bouwstra RJ, GeurtsvanKessel C, van der Eijk AA, Velkers FC, Smit LAM, Stegeman A, van der Poel WHM, Koopmans MPG. Transmission of SARS-CoV-2 on mink farms between humans and mink and back to humans.. Science 2021 Jan 8;371(6525):172-177.
    doi: 10.1126/science.abe5901pmc: PMC7857398pubmed: 33172935google scholar: lookup
  9. Chandler JC, Bevins SN, Ellis JW, Linder TJ, Tell RM, Jenkins-Moore M, Root JJ, Lenoch JB, Robbe-Austerman S, DeLiberto TJ, Gidlewski T, Kim Torchetti M, Shriner SA. SARS-CoV-2 exposure in wild white-tailed deer (Odocoileus virginianus).. Proc Natl Acad Sci U S A 2021 Nov 23;118(47).
    doi: 10.1073/pnas.2114828118pmc: PMC8617405pubmed: 34732584google scholar: lookup
  10. Zhu N, Zhang D, Wang W, Li X, Yang B, Song J, Zhao X, Huang B, Shi W, Lu R, Niu P, Zhan F, Ma X, Wang D, Xu W, Wu G, Gao GF, Tan W. A Novel Coronavirus from Patients with Pneumonia in China, 2019.. N Engl J Med 2020 Feb 20;382(8):727-733.
    doi: 10.1056/NEJMoa2001017pmc: PMC7092803pubmed: 31978945google scholar: lookup
  11. Zhou P, Yang XL, Wang XG, Hu B, Zhang L, Zhang W, Si HR, Zhu Y, Li B, Huang CL, Chen HD, Chen J, Luo Y, Guo H, Jiang RD, Liu MQ, Chen Y, Shen XR, Wang X, Zheng XS, Zhao K, Chen QJ, Deng F, Liu LL, Yan B, Zhan FX, Wang YY, Xiao GF, Shi ZL. A pneumonia outbreak associated with a new coronavirus of probable bat origin.. Nature 2020 Mar;579(7798):270-273.
    doi: 10.1038/s41586-020-2012-7pmc: PMC7095418pubmed: 32015507google scholar: lookup
  12. Boni MF, Lemey P, Jiang X, Lam TT, Perry BW, Castoe TA, Rambaut A, Robertson DL. Evolutionary origins of the SARS-CoV-2 sarbecovirus lineage responsible for the COVID-19 pandemic.. Nat Microbiol 2020 Nov;5(11):1408-1417.
    doi: 10.1038/s41564-020-0771-4pubmed: 32724171google scholar: lookup
  13. Lam TT, Jia N, Zhang YW, Shum MH, Jiang JF, Zhu HC, Tong YG, Shi YX, Ni XB, Liao YS, Li WJ, Jiang BG, Wei W, Yuan TT, Zheng K, Cui XM, Li J, Pei GQ, Qiang X, Cheung WY, Li LF, Sun FF, Qin S, Huang JC, Leung GM, Holmes EC, Hu YL, Guan Y, Cao WC. Identifying SARS-CoV-2-related coronaviruses in Malayan pangolins.. Nature 2020 Jul;583(7815):282-285.
    doi: 10.1038/s41586-020-2169-0pubmed: 32218527google scholar: lookup
  14. Xiao K, Zhai J, Feng Y, Zhou N, Zhang X, Zou JJ, Li N, Guo Y, Li X, Shen X, Zhang Z, Shu F, Huang W, Li Y, Zhang Z, Chen RA, Wu YJ, Peng SM, Huang M, Xie WJ, Cai QH, Hou FH, Chen W, Xiao L, Shen Y. Isolation of SARS-CoV-2-related coronavirus from Malayan pangolins.. Nature 2020 Jul;583(7815):286-289.
    doi: 10.1038/s41586-020-2313-xpubmed: 32380510google scholar: lookup
  15. Lu G, Wang Q, Gao GF. Bat-to-human: spike features determining 'host jump' of coronaviruses SARS-CoV, MERS-CoV, and beyond.. Trends Microbiol 2015 Aug;23(8):468-78.
    doi: 10.1016/j.tim.2015.06.003pmc: PMC7125587pubmed: 26206723google scholar: lookup
  16. Wang Q, Zhang Y, Wu L, Niu S, Song C, Zhang Z, Lu G, Qiao C, Hu Y, Yuen KY, Wang Q, Zhou H, Yan J, Qi J. Structural and Functional Basis of SARS-CoV-2 Entry by Using Human ACE2.. Cell 2020 May 14;181(4):894-904.e9.
    doi: 10.1016/j.cell.2020.03.045pmc: PMC7144619pubmed: 32275855google scholar: lookup
  17. Peng R, Wu LA, Wang Q, Qi J, Gao GF. Cell entry by SARS-CoV-2.. Trends Biochem Sci 2021 Oct;46(10):848-860.
    doi: 10.1016/j.tibs.2021.06.001pmc: PMC8180548pubmed: 34187722google scholar: lookup
  18. Li Y, Wang H, Tang X, Fang S, Ma D, Du C, Wang Y, Pan H, Yao W, Zhang R, Zou X, Zheng J, Xu L, Farzan M, Zhong G. SARS-CoV-2 and Three Related Coronaviruses Utilize Multiple ACE2 Orthologs and Are Potently Blocked by an Improved ACE2-Ig.. J Virol 2020 Oct 27;94(22).
    pmc: PMC7592233pubmed: 32847856doi: 10.1128/jvi.01283-20google scholar: lookup
  19. Wu L, Chen Q, Liu K, Wang J, Han P, Zhang Y, Hu Y, Meng Y, Pan X, Qiao C, Tian S, Du P, Song H, Shi W, Qi J, Wang HW, Yan J, Gao GF, Wang Q. Broad host range of SARS-CoV-2 and the molecular basis for SARS-CoV-2 binding to cat ACE2.. Cell Discov 2020;6:68.
    doi: 10.1038/s41421-020-00210-9pmc: PMC7526519pubmed: 33020722google scholar: lookup
  20. Shi Z, Hu Z. A review of studies on animal reservoirs of the SARS coronavirus.. Virus Res 2008 Apr;133(1):74-87.
    doi: 10.1016/j.virusres.2007.03.012pmc: PMC7114516pubmed: 17451830google scholar: lookup
  21. Liu K, Pan X, Li L, Yu F, Zheng A, Du P, Han P, Meng Y, Zhang Y, Wu L, Chen Q, Song C, Jia Y, Niu S, Lu D, Qiao C, Chen Z, Ma D, Ma X, Tan S, Zhao X, Qi J, Gao GF, Wang Q. Binding and molecular basis of the bat coronavirus RaTG13 virus to ACE2 in humans and other species.. Cell 2021 Jun 24;184(13):3438-3451.e10.
    doi: 10.1016/j.cell.2021.05.031pmc: PMC8142884pubmed: 34139177google scholar: lookup
  22. Liu Y, Hu G, Wang Y, Ren W, Zhao X, Ji F, Zhu Y, Feng F, Gong M, Ju X, Zhu Y, Cai X, Lan J, Guo J, Xie M, Dong L, Zhu Z, Na J, Wu J, Lan X, Xie Y, Wang X, Yuan Z, Zhang R, Ding Q. Functional and genetic analysis of viral receptor ACE2 orthologs reveals a broad potential host range of SARS-CoV-2.. Proc Natl Acad Sci U S A 2021 Mar 23;118(12).
    doi: 10.1073/pnas.2025373118pmc: PMC8000431pubmed: 33658332google scholar: lookup
  23. Davis E, Rush BR, Cox J, DeBey B, Kapil S. Neonatal enterocolitis associated with coronavirus infection in a foal: a case report.. J Vet Diagn Invest 2000 Mar;12(2):153-6.
    doi: 10.1177/104063870001200210pubmed: 10730946google scholar: lookup
  24. Goodrich EL, Mittel LD, Glaser A, Ness SL, Radcliffe RM, Divers TJ. Novel findings from a beta coronavirus outbreak on an American Miniature Horse breeding farm in upstate New York.. Equine Vet Educ 2020 Mar;32(3):150-154.
    doi: 10.1111/eve.12938pmc: PMC7163602pubmed: 32313400google scholar: lookup
  25. Oue Y, Ishihara R, Edamatsu H, Morita Y, Yoshida M, Yoshima M, Hatama S, Murakami K, Kanno T. Isolation of an equine coronavirus from adult horses with pyrogenic and enteric disease and its antigenic and genomic characterization in comparison with the NC99 strain.. Vet Microbiol 2011 May 12;150(1-2):41-8.
    doi: 10.1016/j.vetmic.2011.01.004pmc: PMC7117184pubmed: 21273011google scholar: lookup
  26. Oue Y, Morita Y, Kondo T, Nemoto M. Epidemic of equine coronavirus at Obihiro Racecourse, Hokkaido, Japan in 2012.. J Vet Med Sci 2013;75(9):1261-5.
    doi: 10.1292/jvms.13-0056pubmed: 23648375google scholar: lookup
  27. Pusterla N, Mapes S, Wademan C, White A, Ball R, Sapp K, Burns P, Ormond C, Butterworth K, Bartol J, Magdesian KG. Emerging outbreaks associated with equine coronavirus in adult horses.. Vet Microbiol 2013 Feb 22;162(1):228-31.
    doi: 10.1016/j.vetmic.2012.10.014pmc: PMC7117461pubmed: 23123176google scholar: lookup
  28. Slovis NM, Elam J, Estrada M, Leutenegger CM. Infectious agents associated with diarrhoea in neonatal foals in central Kentucky: a comprehensive molecular study.. Equine Vet J 2014 May;46(3):311-6.
    doi: 10.1111/evj.12119pmc: PMC7163618pubmed: 23773143google scholar: lookup
  29. Su S, Wong G, Shi W, Liu J, Lai ACK, Zhou J, Liu W, Bi Y, Gao GF. Epidemiology, Genetic Recombination, and Pathogenesis of Coronaviruses.. Trends Microbiol 2016 Jun;24(6):490-502.
    doi: 10.1016/j.tim.2016.03.003pmc: PMC7125511pubmed: 27012512google scholar: lookup
  30. Huang C, Liu WJ, Xu W, Jin T, Zhao Y, Song J, Shi Y, Ji W, Jia H, Zhou Y, Wen H, Zhao H, Liu H, Li H, Wang Q, Wu Y, Wang L, Liu D, Liu G, Yu H, Holmes EC, Lu L, Gao GF. A Bat-Derived Putative Cross-Family Recombinant Coronavirus with a Reovirus Gene.. PLoS Pathog 2016 Sep;12(9):e1005883.
    doi: 10.1371/journal.ppat.1005883pmc: PMC5038965pubmed: 27676249google scholar: lookup
  31. Li J, Lai S, Gao GF, Shi W. The emergence, genomic diversity and global spread of SARS-CoV-2.. Nature 2021 Dec;600(7889):408-418.
    doi: 10.1038/s41586-021-04188-6pubmed: 34880490google scholar: lookup
  32. Araf Y, Akter F, Tang YD, Fatemi R, Parvez MSA, Zheng C, Hossain MG. Omicron variant of SARS-CoV-2: Genomics, transmissibility, and responses to current COVID-19 vaccines.. J Med Virol 2022 May;94(5):1825-1832.
    doi: 10.1002/jmv.27588pmc: PMC9015557pubmed: 35023191google scholar: lookup
  33. Xu Z, Liu K, Gao GF. Omicron variant of SARS-CoV-2 imposes a new challenge for the global public health.. Biosaf Health 2022 Jun;4(3):147-149.
    pmc: PMC8767758pubmed: 35072038doi: 10.1016/j.bsheal.2022.01.002google scholar: lookup
  34. Han P, Li L, Liu S, Wang Q, Zhang D, Xu Z, Han P, Li X, Peng Q, Su C, Huang B, Li D, Zhang R, Tian M, Fu L, Gao Y, Zhao X, Liu K, Qi J, Gao GF, Wang P. Receptor binding and complex structures of human ACE2 to spike RBD from omicron and delta SARS-CoV-2.. Cell 2022 Feb 17;185(4):630-640.e10.
    doi: 10.1016/j.cell.2022.01.001pmc: PMC8733278pubmed: 35093192google scholar: lookup
  35. Wrobel AG, Benton DJ, Xu P, Roustan C, Martin SR, Rosenthal PB, Skehel JJ, Gamblin SJ. SARS-CoV-2 and bat RaTG13 spike glycoprotein structures inform on virus evolution and furin-cleavage effects.. Nat Struct Mol Biol 2020 Aug;27(8):763-767.
    doi: 10.1038/s41594-020-0468-7pmc: PMC7610980pubmed: 32647346google scholar: lookup
  36. Lan J, Ge J, Yu J, Shan S, Zhou H, Fan S, Zhang Q, Shi X, Wang Q, Zhang L, Wang X. Structure of the SARS-CoV-2 spike receptor-binding domain bound to the ACE2 receptor.. Nature 2020 May;581(7807):215-220.
    doi: 10.1038/s41586-020-2180-5pubmed: 32225176google scholar: lookup
  37. Shang J, Ye G, Shi K, Wan Y, Luo C, Aihara H, Geng Q, Auerbach A, Li F. Structural basis of receptor recognition by SARS-CoV-2.. Nature 2020 May;581(7807):221-224.
    doi: 10.1038/s41586-020-2179-ypmc: PMC7328981pubmed: 32225175google scholar: lookup
  38. Wu L. Molecular basis of pangolin ACE2 engaged by COVID-19 virus. Chin. Sci. Bull. 2020;66:73–84.
    doi: 10.1360/TB-2020-1372google scholar: lookup
  39. Liu K, Tan S, Niu S, Wang J, Wu L, Sun H, Zhang Y, Pan X, Qu X, Du P, Meng Y, Jia Y, Chen Q, Deng C, Yan J, Wang HW, Wang Q, Qi J, Gao GF. Cross-species recognition of SARS-CoV-2 to bat ACE2.. Proc Natl Acad Sci U S A 2021 Jan 5;118(1).
    doi: 10.1073/pnas.2020216118pmc: PMC7817217pubmed: 33335073google scholar: lookup
  40. Niu S, Wang J, Bai B, Wu L, Zheng A, Chen Q, Du P, Han P, Zhang Y, Jia Y, Qiao C, Qi J, Tian WX, Wang HW, Wang Q, Gao GF. Molecular basis of cross-species ACE2 interactions with SARS-CoV-2-like viruses of pangolin origin.. EMBO J 2021 Aug 16;40(16):e107786.
    doi: 10.15252/embj.2021107786pmc: PMC8209949pubmed: 34018203google scholar: lookup
  41. Han P, Su C, Zhang Y, Bai C, Zheng A, Qiao C, Wang Q, Niu S, Chen Q, Zhang Y, Li W, Liao H, Li J, Zhang Z, Cho H, Yang M, Rong X, Hu Y, Huang N, Yan J, Wang Q, Zhao X, Gao GF, Qi J. Molecular insights into receptor binding of recent emerging SARS-CoV-2 variants.. Nat Commun 2021 Oct 20;12(1):6103.
    doi: 10.1038/s41467-021-26401-wpmc: PMC8528823pubmed: 34671049google scholar: lookup
  42. Mannar D, Saville JW, Zhu X, Srivastava SS, Berezuk AM, Tuttle KS, Marquez AC, Sekirov I, Subramaniam S. SARS-CoV-2 Omicron variant: Antibody evasion and cryo-EM structure of spike protein-ACE2 complex.. Science 2022 Feb 18;375(6582):760-764.
    doi: 10.1126/science.abn7760pmc: PMC9799367pubmed: 35050643google scholar: lookup
  43. Yin W, Xu Y, Xu P, Cao X, Wu C, Gu C, He X, Wang X, Huang S, Yuan Q, Wu K, Hu W, Huang Z, Liu J, Wang Z, Jia F, Xia K, Liu P, Wang X, Song B, Zheng J, Jiang H, Cheng X, Jiang Y, Deng SJ, Xu HE. Structures of the Omicron spike trimer with ACE2 and an anti-Omicron antibody.. Science 2022 Mar 4;375(6584):1048-1053.
    doi: 10.1126/science.abn8863pmc: PMC8939775pubmed: 35133176google scholar: lookup
  44. Gu H, Chen Q, Yang G, He L, Fan H, Deng YQ, Wang Y, Teng Y, Zhao Z, Cui Y, Li Y, Li XF, Li J, Zhang NN, Yang X, Chen S, Guo Y, Zhao G, Wang X, Luo DY, Wang H, Yang X, Li Y, Han G, He Y, Zhou X, Geng S, Sheng X, Jiang S, Sun S, Qin CF, Zhou Y. Adaptation of SARS-CoV-2 in BALB/c mice for testing vaccine efficacy.. Science 2020 Sep 25;369(6511):1603-1607.
    doi: 10.1126/science.abc4730pmc: PMC7574913pubmed: 32732280google scholar: lookup
  45. Zhang Z, Zhang Y, Liu K, Li Y, Lu Q, Wang Q, Zhang Y, Wang L, Liao H, Zheng A, Ma S, Fan Z, Li H, Huang W, Bi Y, Zhao X, Wang Q, Gao GF, Xiao H, Tong Z, Qi J, Sun Y. The molecular basis for SARS-CoV-2 binding to dog ACE2.. Nat Commun 2021 Jul 7;12(1):4195.
    doi: 10.1038/s41467-021-24326-ypmc: PMC8263772pubmed: 34234119google scholar: lookup
  46. Rappazzo CG, Tse LV, Kaku CI, Wrapp D, Sakharkar M, Huang D, Deveau LM, Yockachonis TJ, Herbert AS, Battles MB, O'Brien CM, Brown ME, Geoghegan JC, Belk J, Peng L, Yang L, Hou Y, Scobey TD, Burton DR, Nemazee D, Dye JM, Voss JE, Gunn BM, McLellan JS, Baric RS, Gralinski LE, Walker LM. Broad and potent activity against SARS-like viruses by an engineered human monoclonal antibody.. Science 2021 Feb 19;371(6531):823-829.
    doi: 10.1126/science.abf4830pmc: PMC7963221pubmed: 33495307google scholar: lookup
  47. Liu H, Wu NC, Yuan M, Bangaru S, Torres JL, Caniels TG, van Schooten J, Zhu X, Lee CD, Brouwer PJM, van Gils MJ, Sanders RW, Ward AB, Wilson IA. Cross-Neutralization of a SARS-CoV-2 Antibody to a Functionally Conserved Site Is Mediated by Avidity.. Immunity 2020 Dec 15;53(6):1272-1280.e5.
    doi: 10.1016/j.immuni.2020.10.023pmc: PMC7687367pubmed: 33242394google scholar: lookup
  48. Shi R, Shan C, Duan X, Chen Z, Liu P, Song J, Song T, Bi X, Han C, Wu L, Gao G, Hu X, Zhang Y, Tong Z, Huang W, Liu WJ, Wu G, Zhang B, Wang L, Qi J, Feng H, Wang FS, Wang Q, Gao GF, Yuan Z, Yan J. A human neutralizing antibody targets the receptor-binding site of SARS-CoV-2.. Nature 2020 Aug;584(7819):120-124.
    doi: 10.1038/s41586-020-2381-ypubmed: 32454512google scholar: lookup
  49. Conceicao C, Thakur N, Human S, Kelly JT, Logan L, Bialy D, Bhat S, Stevenson-Leggett P, Zagrajek AK, Hollinghurst P, Varga M, Tsirigoti C, Tully M, Chiu C, Moffat K, Silesian AP, Hammond JA, Maier HJ, Bickerton E, Shelton H, Dietrich I, Graham SC, Bailey D. The SARS-CoV-2 Spike protein has a broad tropism for mammalian ACE2 proteins.. PLoS Biol 2020 Dec;18(12):e3001016.
    doi: 10.1371/journal.pbio.3001016pmc: PMC7751883pubmed: 33347434google scholar: lookup
  50. Mykytyn AZ, Lamers MM, Okba NMA, Breugem TI, Schipper D, van den Doel PB, van Run P, van Amerongen G, de Waal L, Koopmans MPG, Stittelaar KJ, van den Brand JMA, Haagmans BL. Susceptibility of rabbits to SARS-CoV-2.. Emerg Microbes Infect 2021 Dec;10(1):1-7.
    doi: 10.1080/22221751.2020.1868951pmc: PMC7832544pubmed: 33356979google scholar: lookup
  51. Huang K, Zhang Y, Hui X, Zhao Y, Gong W, Wang T, Zhang S, Yang Y, Deng F, Zhang Q, Chen X, Yang Y, Sun X, Chen H, Tao YJ, Zou Z, Jin M. Q493K and Q498H substitutions in Spike promote adaptation of SARS-CoV-2 in mice.. EBioMedicine 2021 May;67:103381.
    doi: 10.1016/j.ebiom.2021.103381pmc: PMC8118724pubmed: 33993052google scholar: lookup
  52. Zhang YN, Zhang ZR, Zhang HQ, Li N, Zhang QY, Li XD, Deng CL, Deng F, Shen S, Zhu B, Zhang B. Different pathogenesis of SARS-CoV-2 Omicron variant in wild-type laboratory mice and hamsters.. Signal Transduct Target Ther 2022 Feb 25;7(1):62.
    doi: 10.1038/s41392-022-00930-2pmc: PMC8873353pubmed: 35217641google scholar: lookup
  53. Kuchipudi SV, Surendran-Nair M, Ruden RM, Yon M, Nissly RH, Vandegrift KJ, Nelli RK, Li L, Jayarao BM, Maranas CD, Levine N, Willgert K, Conlan AJK, Olsen RJ, Davis JJ, Musser JM, Hudson PJ, Kapur V. Multiple spillovers from humans and onward transmission of SARS-CoV-2 in white-tailed deer.. Proc Natl Acad Sci U S A 2022 Feb 8;119(6).
    doi: 10.1073/pnas.2121644119pmc: PMC8833191pubmed: 35078920google scholar: lookup
  54. Otwinowski Z, Minor W. Processing of X-ray diffraction data collected in oscillation mode.. Methods Enzymol 1997;276:307-26.
    doi: 10.1016/S0076-6879(97)76066-Xpubmed: 27754618google scholar: lookup
  55. Adams PD, Afonine PV, Bunkóczi G, Chen VB, Davis IW, Echols N, Headd JJ, Hung LW, Kapral GJ, Grosse-Kunstleve RW, McCoy AJ, Moriarty NW, Oeffner R, Read RJ, Richardson DC, Richardson JS, Terwilliger TC, Zwart PH. PHENIX: a comprehensive Python-based system for macromolecular structure solution.. Acta Crystallogr D Biol Crystallogr 2010 Feb;66(Pt 2):213-21.
    doi: 10.1107/S0907444909052925pmc: PMC2815670pubmed: 20124702google scholar: lookup
  56. Emsley P, Lohkamp B, Scott WG, Cowtan K. Features and development of Coot.. Acta Crystallogr D Biol Crystallogr 2010 Apr;66(Pt 4):486-501.
    doi: 10.1107/S0907444910007493pmc: PMC2852313pubmed: 20383002google scholar: lookup
  57. Chen VB, Arendall WB 3rd, Headd JJ, Keedy DA, Immormino RM, Kapral GJ, Murray LW, Richardson JS, Richardson DC. MolProbity: all-atom structure validation for macromolecular crystallography.. Acta Crystallogr D Biol Crystallogr 2010 Jan;66(Pt 1):12-21.
    doi: 10.1107/S0907444909042073pmc: PMC2803126pubmed: 20057044google scholar: lookup

Citations

This article has been cited 19 times.
  1. Peka M, Balatsky V. Analysis of RBD-ACE2 interactions in livestock species as a factor in the spread of SARS-CoV-2 among animals. Vet Anim Sci 2023 Sep;21:100303.
    doi: 10.1016/j.vas.2023.100303pubmed: 37521409google scholar: lookup
  2. Zhao Z, Xie Y, Bai B, Luo C, Zhou J, Li W, Meng Y, Li L, Li D, Li X, Li X, Wang X, Sun J, Xu Z, Sun Y, Zhang W, Fan Z, Zhao X, Wu L, Ma J, Li OY, Shang G, Chai Y, Liu K, Wang P, Gao GF, Qi J. Structural basis for receptor binding and broader interspecies receptor recognition of currently circulating Omicron sub-variants. Nat Commun 2023 Jul 21;14(1):4405.
    doi: 10.1038/s41467-023-39942-zpubmed: 37479708google scholar: lookup
  3. Huang XY, Chen Q, Sun MX, Zhou HY, Ye Q, Chen W, Peng JY, Qi YN, Zhai JQ, Tian Y, Liu ZX, Huang YJ, Deng YQ, Li XF, Wu A, Yang X, Yang G, Shen Y, Qin CF. A pangolin-origin SARS-CoV-2-related coronavirus: infectivity, pathogenicity, and cross-protection by preexisting immunity. Cell Discov 2023 Jun 17;9(1):59.
    doi: 10.1038/s41421-023-00557-9pubmed: 37330497google scholar: lookup
  4. Guo L, Lin S, Chen Z, Cao Y, He B, Lu G. Targetable elements in SARS-CoV-2 S2 subunit for the design of pan-coronavirus fusion inhibitors and vaccines. Signal Transduct Target Ther 2023 May 10;8(1):197.
    doi: 10.1038/s41392-023-01472-xpubmed: 37164987google scholar: lookup
  5. Nawrath P, Wrobel AG. Hold your horses: The receptor-binding domains of SARS-CoV-2, SARS-CoV, and hCoV-NL63 bind equine ACE2. Structure 2022 Oct 6;30(10):1367-1368.
    doi: 10.1016/j.str.2022.09.003pubmed: 36206735google scholar: lookup
  6. Zhang J, Han ZB, Liang Y, Zhang XF, Jin YQ, Du LF, Shao S, Wang H, Hou JW, Xu K, Lei W, Lei ZH, Liu ZM, Zhang J, Hou YN, Liu N, Shen FJ, Wu JJ, Zheng X, Li XY, Li X, Huang WJ, Wu GZ, Su JG, Li QM. A mosaic-type trimeric RBD-based COVID-19 vaccine candidate induces potent neutralization against Omicron and other SARS-CoV-2 variants. Elife 2022 Aug 25;11.
    doi: 10.7554/eLife.78633pubmed: 36004719google scholar: lookup
  7. Xu Z, Li L, Gu Y, Li D, Qi J, Liu K, Deng C-X, Gao GF. CX1/BtSY2 and BANAL-20-52 exhibit broader receptor binding and higher affinities to multiple animal ACE2 orthologs than SARS-CoV-2 prototype. J Virol 2025 Aug 19;99(8):e0028325.
    doi: 10.1128/jvi.00283-25pubmed: 40637421google scholar: lookup
  8. Lee CY, Huang CW, De Falco L Jr, Minhat RA, Traversier A, Wang B, Mohd Salleh SN, Ngoh EZX, Huang Y, Kim J, Tay MZ, Rosa-Calatrava M, Pizzorno A, Huber RG, Wang CI. A combinatorial and computational Tandem approach towards a universal therapeutics against ACE2-mediated coronavirus infections. iScience 2025 Jun 20;28(6):112687.
    doi: 10.1016/j.isci.2025.112687pubmed: 40520104google scholar: lookup
  9. Guo L, Chen Z, Lin S, Yang F, Yang J, Wang L, Zhang X, Yuan X, He B, Cao Y, Li J, Zhao Q, Lu G. Structural basis and mode of action for two broadly neutralizing nanobodies targeting the highly conserved spike stem-helix of sarbecoviruses including SARS-CoV-2 and its variants. PLoS Pathog 2025 Apr;21(4):e1013034.
    doi: 10.1371/journal.ppat.1013034pubmed: 40215243google scholar: lookup
  10. Luo C, Li L, Gu Y, Zhang H, Xu Z, Sun J, Shi K, Ma S, Tian WX, Liu K, Gao GF. Receptor binding and structural basis of raccoon dog ACE2 binding to SARS-CoV-2 prototype and its variants. PLoS Pathog 2024 Dec;20(12):e1012713.
    doi: 10.1371/journal.ppat.1012713pubmed: 39637248google scholar: lookup
  11. Hu Y, Chen J, Yang J, Liu Z, Zhang X, Wu Q, Liu L, Teng S, He R, Liu B, Zheng X, Lu R, Pan D, Wang Y, Peng L, Chen H, Li YP, Liu W, Qu X. Isolation and characterization of spike S2-specific monoclonal antibodies with reactivity to pan-coronaviruses. Virol Sin 2023 Oct 27;39(1):169-72.
    doi: 10.1016/j.virs.2023.10.008pubmed: 39491181google scholar: lookup
  12. Sasaki D, Arai T, Yang Y, Kuramochi M, Furuyama W, Nanbo A, Sekiguchi H, Morone N, Mio K, Sasaki YC. Micro-second time-resolved X-ray single-molecule internal motions of SARS-CoV-2 spike variants. Biochem Biophys Rep 2024 Jul;38:101712.
    doi: 10.1016/j.bbrep.2024.101712pubmed: 38903159google scholar: lookup
  13. Chen J, Sun J, Xu Z, Li L, Kang X, Luo C, Wang Q, Guo X, Li Y, Liu K, Wu Y. The binding and structural basis of fox ACE2 to RBDs from different sarbecoviruses. Virol Sin 2024 Aug;39(4):609-618.
    doi: 10.1016/j.virs.2024.06.004pubmed: 38866203google scholar: lookup
  14. Hsueh FC, Shi K, Mendoza A, Bu F, Zhang W, Aihara H, Li F. Structural basis for raccoon dog receptor recognition by SARS-CoV-2. PLoS Pathog 2024 May;20(5):e1012204.
    doi: 10.1371/journal.ppat.1012204pubmed: 38709834google scholar: lookup
  15. Peng C, Lv X, Zhang Z, Lin J, Li D. The Recognition Pathway of the SARS-CoV-2 Spike Receptor-Binding Domain to Human Angiotensin-Converting Enzyme 2. Molecules 2024 Apr 19;29(8).
    doi: 10.3390/molecules29081875pubmed: 38675695google scholar: lookup
  16. Yang R, Han P, Han P, Li D, Zhao R, Niu S, Liu K, Li S, Tian W-X, Gao GF. Molecular basis of hippopotamus ACE2 binding to SARS-CoV-2. J Virol 2024 May 14;98(5):e0045124.
    doi: 10.1128/jvi.00451-24pubmed: 38591877google scholar: lookup
  17. Li W, Xu Z, Niu T, Xie Y, Zhao Z, Li D, He Q, Sun W, Shi K, Guo W, Chang Z, Liu K, Fan Z, Qi J, Gao GF. Key mechanistic features of the trade-off between antibody escape and host cell binding in the SARS-CoV-2 Omicron variant spike proteins. EMBO J 2024 Apr;43(8):1484-1498.
    doi: 10.1038/s44318-024-00062-zpubmed: 38467833google scholar: lookup
  18. Shi K, Li L, Luo C, Xu Z, Huang B, Ma S, Liu K, Yu G, Gao GF. Structural basis of increased binding affinities of spikes from SARS-CoV-2 Omicron variants to rabbit and hare ACE2s reveals the expanding host tendency. mBio 2024 Feb 14;15(2):e0298823.
    doi: 10.1128/mbio.02988-23pubmed: 38112468google scholar: lookup
  19. Han P, Meng Y, Zhang D, Xu Z, Li Z, Pan X, Zhao Z, Li L, Tang L, Qi J, Liu K, Gao GF. Structural basis of white-tailed deer, Odocoileus virginianus, ACE2 recognizing all the SARS-CoV-2 variants of concern with high affinity. J Virol 2023 Sep 28;97(9):e0050523.
    doi: 10.1128/jvi.00505-23pubmed: 37676003google scholar: lookup
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