Biochemical differences between distal limb extensor and flexor tendons among equine breeds selected for racing and sport.
Abstract: Throughout the ages, humans have selected different horse breeds for their locomotor capacities. Consequently, the properties of equine locomotor tissues could have diversified because of the specific requirements of different disciplines. Therefore, this study aimed to compare biochemical properties of tendons in different equine breeds traditionally selected for racing or sports performance. We hypothesised that tendons in racing breeds would have biochemical properties that would increase strength, whereas those in sporting breeds would have more elastic properties. An ex vivo tendon tissue study comparing the common digital extensor tendon (CDET) and superficial digital flexor tendon (SDFT) of sports horses (Friesian horse, Warmblood horse) and racehorses (Thoroughbred horse; the oldest, reference standard breed) was performed. The SDFT and CDET from middle-aged Friesian (n = 12), Warmblood (n = 12) and Thoroughbred horses (n = 8) were harvested, and their biochemical properties were compared. The biochemical analysis demonstrated significantly higher water percentage, lower collagen concentrations/glycosaminoglycan content and higher crosslink concentrations in the SDFT of sports horses compared to racing breed horses (P < 0.05); DNA content was also significantly lower in sports horses than racehorses (P < 0.05). Racehorses had mainly extra fibrillar collagen support, whereas sports horses had mainly extra crosslink collagen support. From a functional perspective, the racing Thoroughbred relied on stronger tendons, while the sporting Friesians and Warmbloods relied on less stiff, more elastic tendons. In conclusion, there were significant biochemical differences in tendon properties between breeds, possibly related to their intended locomotor performance, although this requires further biomechanical and ultimately genetic confirmation.
Copyright © 2020 Elsevier Ltd. All rights reserved.
Publication Date: 2020-07-10 PubMed ID: 32792094DOI: 10.1016/j.tvjl.2020.105515Google Scholar: Lookup
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Summary
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This research study focuses on the comparison of the biochemical properties of tendons in horse breeds traditionally selected for racing or sports performance. It establishes that there are significant biochemical differences in tendon properties between these breeds, which can be linked to their specific locomotor functions.
Objective of the Study
- The primary goal of the research was to investigate the potential biochemical differences between tendons of different horse breeds particularly chosen for racing and sports performance.
- The hypothesis was that racing breeds’ tendons would exhibit properties that increase strength, whereas those in sports-performing breeds would be more elastic.
Methodology
- The researchers conducted an ex vivo tendon tissue study where they compared the tendons from sports horses (specifically, the Friesian and Warmblood horses) and racehorses (the Thoroughbred breed, which is often considered the reference standard).
- The Common Digital Extensor Tendon (CDET) and the Superficial Digital Flexor Tendon (SDFT) of these horses, all of middle age, were harvested and analyzed biochemically.
Findings
- The study revealed that the SDFT of sports horses had a significantly higher water percentage, lower collagen concentrations/glycosaminoglycan content, and higher crosslink concentrations compared to those of racehorses.
- The DNA content was also markedly lower in sports horses than in racehorses.
- Racing breed, Thoroughbreds, were found to rely mostly on extra fibrillar collagen support, while sports breeds, the Friesians and Warmbloods, leaned more toward extra crosslink collagen support.
- These observations pointed toward the racing Thoroughbred having stronger tendons, while the sporting Friesians and Warmbloods utilized less stiff, more elastic tendons.
Conclusion
- The research concludes that significant biochemical differences exist in the properties of tendons between racing and sports horse breeds.
- These differences can largely be correlated to the chosen locomotor performance of each breed; stronger tendons for racing and elasticity for sports performance.
- The findings, however, request further biomechanical and genetic confirmation.
Cite This Article
APA
Verkade ME, Hazeleger E, van de Lest CHA, Back W.
(2020).
Biochemical differences between distal limb extensor and flexor tendons among equine breeds selected for racing and sport.
Vet J, 262, 105515.
https://doi.org/10.1016/j.tvjl.2020.105515 Publication
Researcher Affiliations
- Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 112-114, NL-3584 CM Utrecht, The Netherlands. Electronic address: maria.verkade@vetagro-sup.fr.
- Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 112-114, NL-3584 CM Utrecht, The Netherlands.
- Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 112-114, NL-3584 CM Utrecht, The Netherlands; Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 2, NL-3584 CM Utrecht, The Netherlands.
- Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 112-114, NL-3584 CM Utrecht, The Netherlands; Department of Surgery and Anaesthesiology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
MeSH Terms
- Animals
- Collagen / analysis
- Glycosaminoglycans / analysis
- Hindlimb / chemistry
- Horses
- Selection, Genetic
- Sports
- Tendons / chemistry
Citations
This article has been cited 2 times.- Giessen EJM, Stas EKL, Grinwis GCM, Veraa S. Imaging Findings of Congenital Distal Interphalangeal Joint Dysplasia in a 3-Month-Old Friesian Foal. Vet Radiol Ultrasound 2025 Sep;66(5):e70076.
- Shojaee A. Equine tendon mechanical behaviour: Prospects for repair and regeneration applications. Vet Med Sci 2023 Sep;9(5):2053-2069.
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