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Frontiers in veterinary science2016; 3; 23; doi: 10.3389/fvets.2016.00023

Biopsy Needle Advancement during Bone Marrow Aspiration Increases Mesenchymal Stem Cell Concentration.

Abstract: Point-of-care kits to concentrate bone marrow (BM)-derived mesenchymal stem cells (MSCs) are used clinically in horses. A maximal number of MSCs per milliliter of marrow aspirated might be desired prior to use of a point-of-care system to concentrate MSCs. Our objective was to test a method to increase the number of MSCs per milliliter of marrow collected. We collected two BM aspirates using two different collection techniques from 12 horses. The first collection technique was to aspirate BM from a single site without advancement of the biopsy needle. The second collection technique was to aspirate marrow from multiple sites within the same sternal puncture by advancing the needle 5 mm three times for BM aspiration from four sites. Numbers of MSCs in collected BM were assessed by total nucleated cell count of BM after aspiration, total colony-forming unit-fibroblast (CFU-F) assay, and total MSC number at each culture passage. The BM aspiration technique of four needle advancements during BM aspiration resulted in higher initial nucleated cell counts, more CFU-Fs, and more MSCs at the first passage. There were no differences in the number of MSCs at later passages. Multiple advancements of the BM needle during BM aspiration resulted in increased MSC concentration at the time of BM collection. If a point-of-care kit is used to concentrate MSCs, multiple advancements may result in higher MSC numbers in the BM concentrate after preparation by the point-of-care kit. For culture expanded MSCs beyond the first cell passage, the difference is of questionable clinical relevance.
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Publication Date: 2016-03-14 PubMed ID: 27014705PubMed Central: PMC4789557DOI: 10.3389/fvets.2016.00023Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research studied ways to increase the concentration of mesenchymal stem cells (MSCs) in horse bone marrow samples. The researchers found that by advancing the biopsy needle during sample collection, they could increase the initial concentration of MSCs, although the effect diminished in later stages of the MSCs’ development.

Study Objectives and Methods

  • The primary objective of this study was to find a method to enhance the concentration of MSCs per milliliter of bone marrow collected for use with point-of-care systems that concentrate MSCs commonly used in horse treatment.
  • Researchers collected two bone marrow samples from 12 horses using two different collection techniques. In the first method, marrow was aspirated from a single site without advancing the biopsy needle. The second technique involved aspirating marrow from multiple sites within the same sternal puncture by advancing the needle 5mm three times for bone marrow aspiration from four sites.

Outcome Assessment

  • The researchers compared the number of MSCs in the collected bone marrow samples using three measures: the total number of nucleated cells after aspiration, the total number of colony-forming unit-fibroblast (CFU-F), and the total MSC number at each culture passage.

Results and Conclusion

  • The technique of advancing the BM needle during aspiration resulted in higher initial nucleated cell counts, more CFU-Fs, and an increased number of MSCs at the first passage.
  • No significant differences were observed in the number of MSCs at later passages.
  • Therefore, multiple advancements of the BM needle can increase MSC concentration at the time of collection. If a point-of-care kit is used, this technique might help to improve the number of MSCs in the prepared concentrate.
  • However, for cultures expanded beyond the first cell passage, the researchers believe the impact would be negligible and thus of questionable clinical relevance.

Cite This Article

APA
Peters AE, Watts AE. (2016). Biopsy Needle Advancement during Bone Marrow Aspiration Increases Mesenchymal Stem Cell Concentration. Front Vet Sci, 3, 23. https://doi.org/10.3389/fvets.2016.00023

Publication

Frontiers in veterinary science
ISSN: 2297-1769
NlmUniqueID: 101666658
Country: Switzerland
Language: English
Volume: 3
Pages: 23
PII: 23

Researcher Affiliations

Peters, Anne E
  • Department of Large Animal Clinical Sciences, Texas A&M University , College Station, TX , USA.
Watts, Ashlee E
  • Department of Large Animal Clinical Sciences, Texas A&M University , College Station, TX , USA.

References

This article includes 22 references
  1. Herthel D. Enhanced suspensory ligament healing in 100 horses by stem cells and other bone marrow components. Proc Am Ass Eq Pract (2001) 47:319–21.
  2. Fortier LA, Potter HG, Rickey EJ, Schnabel LV, Foo LF, Chong LR, Stokol T, Cheetham J, Nixon AJ. Concentrated bone marrow aspirate improves full-thickness cartilage repair compared with microfracture in the equine model.. J Bone Joint Surg Am 2010 Aug 18;92(10):1927-37.
    doi: 10.2106/jbjs.i.01284pubmed: 20720135google scholar: lookup
  3. Godwin EE, Young NJ, Dudhia J, Beamish IC, Smith RK. Implantation of bone marrow-derived mesenchymal stem cells demonstrates improved outcome in horses with overstrain injury of the superficial digital flexor tendon.. Equine Vet J 2012 Jan;44(1):25-32.
    doi: 10.1111/j.2042-3306.2011.00363.xpubmed: 21615465google scholar: lookup
  4. Ikebe C, Suzuki K. Mesenchymal stem cells for regenerative therapy: optimization of cell preparation protocols.. Biomed Res Int 2014;2014:951512.
    doi: 10.1155/2014/951512pmc: PMC3912818pubmed: 24511552google scholar: lookup
  5. Connolly J, Guse R, Lippiello L, Dehne R. Development of an osteogenic bone-marrow preparation.. J Bone Joint Surg Am 1989 Jun;71(5):684-91.
    pubmed: 2732257
  6. Hermann PC, Huber SL, Herrler T, von Hesler C, Andrassy J, Kevy SV, Jacobson MS, Heeschen C. Concentration of bone marrow total nucleated cells by a point-of-care device provides a high yield and preserves their functional activity.. Cell Transplant 2008;16(10):1059-69.
    doi: 10.3727/000000007783472363pubmed: 18351022google scholar: lookup
  7. Thoesen MS, Berg-Foels WS, Stokol T, Rassnick KM, Jacobson MS, Kevy SV, Todhunter RJ. Use of a centrifugation-based, point-of-care device for production of canine autologous bone marrow and platelet concentrates.. Am J Vet Res 2006 Oct;67(10):1655-61.
    doi: 10.2460/ajvr.67.10.1655pubmed: 17014312google scholar: lookup
  8. Hakimi M, Grassmann JP, Betsch M, Schneppendahl J, Gehrmann S, Hakimi AR, Kröpil P, Sager M, Herten M, Wild M, Windolf J, Jungbluth P. The composite of bone marrow concentrate and PRP as an alternative to autologous bone grafting.. PLoS One 2014;9(6):e100143.
    doi: 10.1371/journal.pone.0100143pmc: PMC4064995pubmed: 24950251google scholar: lookup
  9. Ishihara A, Helbig HJ, Sanchez-Hodge RB, Wellman ML, Landrigan MD, Bertone AL. Performance of a gravitational marrow separator, multidirectional bone marrow aspiration needle, and repeated bone marrow collections on the production of concentrated bone marrow and separation of mesenchymal stem cells in horses.. Am J Vet Res 2013 Jun;74(6):854-63.
    doi: 10.2460/ajvr.74.6.854pubmed: 23718653google scholar: lookup
  10. Hernigou P, Mathieu G, Poignard A, Manicom O, Beaujean F, Rouard H. Percutaneous autologous bone-marrow grafting for nonunions. Surgical technique.. J Bone Joint Surg Am 2006 Sep;88 Suppl 1 Pt 2:322-7.
    doi: 10.2106/jbjs.f.00203pubmed: 16951103google scholar: lookup
  11. Lee DH, Ryu KJ, Kim JW, Kang KC, Choi YR. Bone marrow aspirate concentrate and platelet-rich plasma enhanced bone healing in distraction osteogenesis of the tibia.. Clin Orthop Relat Res 2014 Dec;472(12):3789-97.
    doi: 10.1007/s11999-014-3548-3pmc: PMC4397746pubmed: 24599650google scholar: lookup
  12. Muschler GF, Boehm C, Easley K. Aspiration to obtain osteoblast progenitor cells from human bone marrow: the influence of aspiration volume.. J Bone Joint Surg Am 1997 Nov;79(11):1699-709.
    pubmed: 9384430doi: 10.2106/00004623-199711000-00012google scholar: lookup
  13. Kasashima Y, Ueno T, Tomita A, Goodship AE, Smith RK. Optimisation of bone marrow aspiration from the equine sternum for the safe recovery of mesenchymal stem cells.. Equine Vet J 2011 May;43(3):288-94.
    doi: 10.1111/j.2042-3306.2010.00215.xpubmed: 21492205google scholar: lookup
  14. Franken NA, Rodermond HM, Stap J, Haveman J, van Bree C. Clonogenic assay of cells in vitro.. Nat Protoc 2006;1(5):2315-9.
    doi: 10.1038/nprot.2006.339pubmed: 17406473google scholar: lookup
  15. Schnabel LV, Pezzanite LM, Antczak DF, Felippe MJ, Fortier LA. Equine bone marrow-derived mesenchymal stromal cells are heterogeneous in MHC class II expression and capable of inciting an immune response in vitro.. Stem Cell Res Ther 2014 Jan 24;5(1):13.
    doi: 10.1186/scrt402pmc: PMC4055004pubmed: 24461709google scholar: lookup
  16. Radcliffe CH, Flaminio MJ, Fortier LA. Temporal analysis of equine bone marrow aspirate during establishment of putative mesenchymal progenitor cell populations.. Stem Cells Dev 2010 Feb;19(2):269-82.
    doi: 10.1089/scd.2009.0091pmc: PMC3138180pubmed: 19604071google scholar: lookup
  17. Grogan SP, Barbero A, Winkelmann V, Rieser F, Fitzsimmons JS, O'Driscoll S, Martin I, Mainil-Varlet P. Visual histological grading system for the evaluation of in vitro-generated neocartilage.. Tissue Eng 2006 Aug;12(8):2141-9.
    doi: 10.1089/ten.2006.12.2141pubmed: 16968155google scholar: lookup
  18. English A, Jones EA, Corscadden D, Henshaw K, Chapman T, Emery P, McGonagle D. A comparative assessment of cartilage and joint fat pad as a potential source of cells for autologous therapy development in knee osteoarthritis.. Rheumatology (Oxford) 2007 Nov;46(11):1676-83.
    doi: 10.1093/rheumatology/kem217pubmed: 17901063google scholar: lookup
  19. Zhu Y, Ouyang Y, Chang Y, Luo C, Xu J, Zhang C, Huang W. Evaluation of the proliferation and differentiation behaviors of mesenchymal stem cells with partially converted borate glass containing different amounts of strontium in vitro.. Mol Med Rep 2013 Apr;7(4):1129-36.
    doi: 10.3892/mmr.2013.1341pubmed: 23446964google scholar: lookup
  20. Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR. Multilineage potential of adult human mesenchymal stem cells.. Science 1999 Apr 2;284(5411):143-7.
    doi: 10.1126/science.284.5411.143pubmed: 10102814google scholar: lookup
  21. Hernigou P, Homma Y, Flouzat Lachaniette CH, Poignard A, Allain J, Chevallier N, Rouard H. Benefits of small volume and small syringe for bone marrow aspirations of mesenchymal stem cells.. Int Orthop 2013 Nov;37(11):2279-87.
    doi: 10.1007/s00264-013-2017-zpmc: PMC3824897pubmed: 23881064google scholar: lookup
  22. Jäger M, Herten M, Fochtmann U, Fischer J, Hernigou P, Zilkens C, Hendrich C, Krauspe R. Bridging the gap: bone marrow aspiration concentrate reduces autologous bone grafting in osseous defects.. J Orthop Res 2011 Feb;29(2):173-80.
    doi: 10.1002/jor.21230pubmed: 20740672google scholar: lookup

Citations

This article has been cited 12 times.
  1. El-Husseiny HM, Mady EA, Helal MAY, Tanaka R. The Pivotal Role of Stem Cells in Veterinary Regenerative Medicine and Tissue Engineering. Vet Sci 2022 Nov 21;9(11).
    doi: 10.3390/vetsci9110648pubmed: 36423096google scholar: lookup
  2. Pasculli RM, Kenyon CD, Berrigan WA, Mautner K, Hammond K, Jayaram P. Mesenchymal stem cells for subchondral bone marrow lesions: From bench to bedside. Bone Rep 2022 Dec;17:101630.
    doi: 10.1016/j.bonr.2022.101630pubmed: 36310763google scholar: lookup
  3. Jeyaraman M, Bingi SK, Muthu S, Jeyaraman N, Packkyarathinam RP, Ranjan R, Sharma S, Jha SK, Khanna M, Rajendran SNS, Rajendran RL, Gangadaran P. Impact of the Process Variables on the Yield of Mesenchymal Stromal Cells from Bone Marrow Aspirate Concentrate. Bioengineering (Basel) 2022 Jan 29;9(2).
    doi: 10.3390/bioengineering9020057pubmed: 35200410google scholar: lookup
  4. Prządka P, Buczak K, Frejlich E, Gąsior L, Suliga K, Kiełbowicz Z. The Role of Mesenchymal Stem Cells (MSCs) in Veterinary Medicine and Their Use in Musculoskeletal Disorders. Biomolecules 2021 Aug 2;11(8).
    doi: 10.3390/biom11081141pubmed: 34439807google scholar: lookup
  5. Ruane JJ, Ross A, Zigmont V, McClure D, Gascon G. A Single-Blinded Randomized Controlled Trial of Mesenchymal Stem Cell Therapy for the Treatment of Osteoarthritis of the Knee with Active Control. J Stem Cells Regen Med 2021;17(1):3-17.
    doi: 10.46582/jsrm.1701002pubmed: 34434003google scholar: lookup
  6. Themistocleous GS, Chloros GD, Kyrantzoulis IM, Georgokostas IA, Themistocleous MS, Papagelopoulos PJ, Savvidou OD. Effectiveness of a single intra-articular bone marrow aspirate concentrate (BMAC) injection in patients with grade 3 and 4 knee osteoarthritis. Heliyon 2018 Oct;4(10):e00871.
    doi: 10.1016/j.heliyon.2018.e00871pubmed: 30364761google scholar: lookup
  7. Bogers SH. Cell-Based Therapies for Joint Disease in Veterinary Medicine: What We Have Learned and What We Need to Know. Front Vet Sci 2018;5:70.
    doi: 10.3389/fvets.2018.00070pubmed: 29713634google scholar: lookup
  8. Gaul F, Bugbee WD, Hoenecke HR Jr, D'Lima DD. A Review of Commercially Available Point-of-Care Devices to Concentrate Bone Marrow for the Treatment of Osteoarthritis and Focal Cartilage Lesions. Cartilage 2019 Oct;10(4):387-394.
    doi: 10.1177/1947603518768080pubmed: 29652173google scholar: lookup
  9. Oliver K, Awan T, Bayes M. Single- Versus Multiple-Site Harvesting Techniques for Bone Marrow Concentrate: Evaluation of Aspirate Quality and Pain. Orthop J Sports Med 2017 Aug;5(8):2325967117724398.
    doi: 10.1177/2325967117724398pubmed: 28890905google scholar: lookup
  10. Joswig AJ, Mitchell A, Cummings KJ, Levine GJ, Gregory CA, Smith R 3rd, Watts AE. Repeated intra-articular injection of allogeneic mesenchymal stem cells causes an adverse response compared to autologous cells in the equine model. Stem Cell Res Ther 2017 Feb 28;8(1):42.
    doi: 10.1186/s13287-017-0503-8pubmed: 28241885google scholar: lookup
  11. Holton J, Imam MA, Snow M. Bone Marrow Aspirate in the Treatment of Chondral Injuries. Front Surg 2016;3:33.
    doi: 10.3389/fsurg.2016.00033pubmed: 27379241google scholar: lookup
  12. Migliorini F, Pilone M, Simeone F, Jeyaraman M, Bell A, Maffulli N. Progress in the clinical use of bone marrow aspirate concentrate for knee osteoarthritis: an expert opinion. J Orthop Surg Res 2025 Dec 10;20(1):1065.
    doi: 10.1186/s13018-025-06509-1pubmed: 41372742google scholar: lookup
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