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Home / Equine Research Bank / Vet Med Sci / Bone marrow-derived multipotent mesenchymal stromal cells fr...
Veterinary medicine and science2017; 3(4); 239-251; doi: 10.1002/vms3.74

Bone marrow-derived multipotent mesenchymal stromal cells from horses after euthanasia.

Abstract: Allogeneic equine multipotent mesenchymal stromal cells (eMSCs) have been proposed for use in regenerative therapies in veterinary medicine. A source of allogeneic eMSCs might be the bone marrow from euthanized horses. The purpose of this study was to compare characteristics of equine bone marrow derived eMSC (eBM-MSCs) from euthanized horses (eut-MSCs) and from narcotized horses (nar-MSCs). Eut-MSCs and nar-MSCs showed typical eMSC marker profiles (positive: CD44, CD90; negative: CD11a/CD18 and MHCII) and possessed tri-lineage differentiation characteristics. Although CD105 and MHCI expression varied, no differences were detected between eut-MSCs and nar-MSCs. Proliferation characteristics did not differ between eut-MSCs and nar-MSCs, but age dependent decrease in proliferation and increase in MHCI expression was detected. These results suggest the possible use of eut-MSCs for therapeutic applications and production of commercial available eBM-MSC products.
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Publication Date: 2017-09-12 PubMed ID: 29152317PubMed Central: PMC5677777DOI: 10.1002/vms3.74Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research studied the characteristics of equine bone marrow-derived multipotent mesenchymal stromal cells (eBM-MSCs) extracted from euthanized horses, intending to assess whether they could serve as a viable source of cells for regenerative therapies in veterinary medicine. The findings indicated similar characteristics and proliferation rates between these cells and those derived from narcotized horses, with an age-dependent decrease in proliferation and increase in MHCI expression.

Introduction and Objective

  • The research aimed to explore potential uses of equine multipotent mesenchymal stromal cells (eMSCs) sourced from the bone marrow of horses after euthanasia, with regenerative therapies in veterinary medicine being a primary objective.

Methodology

  • Researchers compared attributes of equine bone marrow-derived MSCs (eBM-MSCs) obtained from euthanized horses (eut-MSCs) with those extracted from narcotized horses (nar-MSCs).
  • Cells were analyzed for specific markers associated with eMSCs, which include positivity for CD44 and CD90, and negativity for CD11a/CD18 and MHCII.

Findings

  • The under-study cells—eut-MSCs and nar-MSCs—revealed the representative eMSC marker profile and possessed tri-lineage differentiation traits.
  • The expression of CD105 and MHCI varied, although no distinct differences were discovered between eut-MSCs and nar-MSCs.
  • While proliferation traits did not differ between eut-MSCs and nar-MSCs, there was an observable decrease in proliferation and increase in MHCI expression with age.

Conclusion and Implications

  • The results indicate that eBM-MSCs from euthanized horses could be effectively utilized for therapeutic applications, including the manufacturing of commercial eBM-MSC products.
  • As such, these findings could significantly contribute to expanding the resources for veterinary regenerative medicine, thus offering potential new treatments for various equine conditions.

Cite This Article

APA
Schröck C, Eydt C, Geburek F, Kaiser L, Päbst F, Burk J, Pfarrer C, Staszyk C. (2017). Bone marrow-derived multipotent mesenchymal stromal cells from horses after euthanasia. Vet Med Sci, 3(4), 239-251. https://doi.org/10.1002/vms3.74

Publication

Veterinary medicine and science
ISSN: 2053-1095
NlmUniqueID: 101678837
Country: England
Language: English
Volume: 3
Issue: 4
Pages: 239-251

Researcher Affiliations

Schröck, Carmen
  • Institute for Veterinary Anatomy, -Histology and -EmbryologyJustus-Liebig-UniversityGiessenGermany.
Eydt, Carina
  • Institute of AnatomyUniversity of Veterinary Medicine HannoverHannoverGermany.
Geburek, Florian
  • Clinic for HorsesJustus-Liebig-UniversityGiessenGermany.
Kaiser, Lena
  • Institute for Veterinary Anatomy, -Histology and -EmbryologyJustus-Liebig-UniversityGiessenGermany.
Päbst, Felicitas
  • Translational Centre for Regenerative Medicine (TRM)University of LeipzigLeipzigGermany.
  • Faculty of Veterinary MedicineLarge Animal Clinic for SurgeryUniversity of LeipzigLeipzigGermany.
Burk, Janina
  • Translational Centre for Regenerative Medicine (TRM)University of LeipzigLeipzigGermany.
  • Faculty of Veterinary MedicineLarge Animal Clinic for SurgeryUniversity of LeipzigLeipzigGermany.
Pfarrer, Christiane
  • Institute of AnatomyUniversity of Veterinary Medicine HannoverHannoverGermany.
Staszyk, Carsten
  • Institute for Veterinary Anatomy, -Histology and -EmbryologyJustus-Liebig-UniversityGiessenGermany.

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Citations

This article has been cited 3 times.
  1. Bagge J, Berg LC, Janes J, MacLeod JN. Donor age effects on in vitro chondrogenic and osteogenic differentiation performance of equine bone marrow- and adipose tissue-derived mesenchymal stromal cells.. BMC Vet Res 2022 Nov 3;18(1):388.
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  2. Trachsel DS, Stage HJ, Rausch S, Trappe S, Söllig K, Sponder G, Merle R, Aschenbach JR, Gehlen H. Comparison of Sources and Methods for the Isolation of Equine Adipose Tissue-Derived Stromal/Stem Cells and Preliminary Results on Their Reaction to Incubation with 5-Azacytidine.. Animals (Basel) 2022 Aug 11;12(16).
    doi: 10.3390/ani12162049pubmed: 36009640google scholar: lookup
  3. Bagge J, MacLeod JN, Berg LC. Cellular Proliferation of Equine Bone Marrow- and Adipose Tissue-Derived Mesenchymal Stem Cells Decline With Increasing Donor Age.. Front Vet Sci 2020;7:602403.
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