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Home / Equine Research Bank / Int J Mol Sci / Chondrogenic Differentiation of Defined Equine Mesenchymal S...
International journal of molecular sciences2018; 19(2); 537; doi: 10.3390/ijms19020537

Chondrogenic Differentiation of Defined Equine Mesenchymal Stem Cells Derived from Umbilical Cord Blood for Use in Cartilage Repair Therapy.

Abstract: Cartilage engineering is a new strategy for the treatment of cartilage damage due to osteoarthritis or trauma in humans. Racehorses are exposed to the same type of cartilage damage and the anatomical, cellular, and biochemical properties of their cartilage are comparable to those of human cartilage, making the horse an excellent model for the development of cartilage engineering. Human mesenchymal stem cells (MSCs) differentiated into chondrocytes with chondrogenic factors in a biomaterial appears to be a promising therapeutic approach for direct implantation and cartilage repair. Here, we characterized equine umbilical cord blood-derived MSCs (eUCB-MSCs) and evaluated their potential for chondrocyte differentiation for use in cartilage repair therapy. Our results show that isolated eUCB-MSCs had high proliferative capacity and differentiated easily into osteoblasts and chondrocytes, but not into adipocytes. A three-dimensional (3D) culture approach with the chondrogenic factors BMP-2 and TGF-β1 potentiated chondrogenic differentiation with a significant increase in cartilage-specific markers at the mRNA level (, , ) and the protein level (type II and IIB collagen) without an increase in hypertrophic chondrocyte markers ( and ) in normoxia and in hypoxia. However, these chondrogenic factors caused an increase in type I collagen, which can be reduced using small interfering RNA targeting . This study provides robust data on MSCs characterization and demonstrates that eUCB-MSCs have a great potential for cartilage tissue engineering.
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Publication Date: 2018-02-10 PubMed ID: 29439436PubMed Central: PMC5855759DOI: 10.3390/ijms19020537Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study investigates how stem cells from horse umbilical cord blood can be used to repair cartilage damage, a solution that could potentially apply to human cartilage repair as well.

Cartilage Engineering and Stem Cells

  • The study is based on the field of “cartilage engineering”, which explores new treatment methods for damaged cartilage in individuals with diseases like osteoarthritis or those who have suffered from trauma.
  • Researchers have identified cartilage damage in racehorses, with properties similar to damaged human cartilage, making them a viable model for the development of cartilage engineering.
  • The use of human mesenchymal stem cells (MSCs) is seen as a promising approach towards cartilage repair. These cells can differentiate into chondrocytes – the cells forming cartilage – when treated with chondrogenic factors within a specific biomaterial.

Role of Equine Umbilical Cord Blood-derived MSCs (eUCB-MSCs)

  • The paper mainly focuses on characterizing eUCB-MSCs and evaluating their potential for differentiating into chondrocytes for effective cartilage repair.
  • The isolated eUCB-MSCs showed high proliferation capacity and were observed to easily differentiate into osteoblasts and chondrocytes, but not adipocytes, indicating their possible use in cartilage tissue engineering.

Three-Dimensional Culture Approach

  • A three-dimensional (3D) culture approach was used with chondrogenic factors BMP-2 and TGF-β1, two proteins vital for cartilage development and repair, to potentiate chondrogenic differentiation.
  • There was a significant increase in cartilage-specific markers at both mRNA and protein levels after use of the chondrogenic factors within this 3D culture, without an increase in markers for hypertrophic chondrocytes, which can lead to impaired functional properties of the cartilage.
  • However, the use of these factors also resulted in an increase in type I collagen. This is not ideal, as type II collagen is more desirable for cartilage repair. Researchers counteracted this by using small interfering RNA to target COL1A1 gene.

Conclusion

  • The research demonstrates that eUCB-MSCs show huge potential for use in cartilage tissue engineering, providing an encouraging new avenue for the treatment of cartilage damage in humans.

Cite This Article

APA
Desancé M, Contentin R, Bertoni L, Gomez-Leduc T, Branly T, Jacquet S, Betsch JM, Batho A, Legendre F, Audigié F, Galéra P, Demoor M. (2018). Chondrogenic Differentiation of Defined Equine Mesenchymal Stem Cells Derived from Umbilical Cord Blood for Use in Cartilage Repair Therapy. Int J Mol Sci, 19(2), 537. https://doi.org/10.3390/ijms19020537

Publication

International journal of molecular sciences
ISSN: 1422-0067
NlmUniqueID: 101092791
Country: Switzerland
Language: English
Volume: 19
Issue: 2
PII: 537

Researcher Affiliations

Desancé, Mélanie
  • Normandie University, UNICAEN, BIOTARGEN, 14000 Caen, France. melanie359@hotmail.fr.
Contentin, Romain
  • Normandie University, UNICAEN, BIOTARGEN, 14000 Caen, France. romaincontentin@hotmail.fr.
Bertoni, Lélia
  • Center of Imaging and Research on Locomotor Affections in Equines, Ecole Vétérinaire d'Alfort, Université Paris-Est, 14430 Goustranville, France. lelia.bertoni@vet-alfort.fr.
Gomez-Leduc, Tangni
  • Normandie University, UNICAEN, BIOTARGEN, 14000 Caen, France. tangnigl@free.fr.
Branly, Thomas
  • Normandie University, UNICAEN, BIOTARGEN, 14000 Caen, France. tbranly@gmail.com.
Jacquet, Sandrine
  • Center of Imaging and Research on Locomotor Affections in Equines, Ecole Vétérinaire d'Alfort, Université Paris-Est, 14430 Goustranville, France. sandrine.jacquet@vet-alfort.fr.
Betsch, Jean-Marc
  • Clinique Vétérinaire Equine de Méheudin, Méheudin, 61150 Ecouché, France. jmbetsch@cvem.fr.
Batho, Agnès
  • Normandie University, UNICAEN, BIOTARGEN, 14000 Caen, France. Agnes.BATHO@efs.sante.fr.
  • EFS Caen, 14000 Caen, France. Agnes.BATHO@efs.sante.fr.
Legendre, Florence
  • Normandie University, UNICAEN, BIOTARGEN, 14000 Caen, France. florence.legendre@unicaen.fr.
Audigié, Fabrice
  • Center of Imaging and Research on Locomotor Affections in Equines, Ecole Vétérinaire d'Alfort, Université Paris-Est, 14430 Goustranville, France. fabrice.audigie@vet-alfort.fr.
Galéra, Philippe
  • Normandie University, UNICAEN, BIOTARGEN, 14000 Caen, France. philippe.galera@unicaen.fr.
Demoor, Magali
  • Normandie University, UNICAEN, BIOTARGEN, 14000 Caen, France. magali.demoor@unicaen.fr.

MeSH Terms

  • Animals
  • Bone Morphogenetic Protein 2 / pharmacology
  • Cartilage / physiology
  • Cell Differentiation
  • Cells, Cultured
  • Chondrocytes / cytology
  • Chondrocytes / metabolism
  • Chondrogenesis
  • Collagen / genetics
  • Collagen / metabolism
  • Fetal Blood / cytology
  • Horses
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / drug effects
  • Mesenchymal Stem Cells / metabolism
  • Regeneration
  • Transforming Growth Factor beta1 / pharmacology

Conflict of Interest Statement

The authors declare no conflict of interest.

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