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Genes2021; 12(12); doi: 10.3390/genes12121965

Circulating Transcriptional Profile Modulation in Response to Metabolic Unbalance Due to Long-Term Exercise in Equine Athletes: A Pilot Study.

Abstract: Physical exercise has been associated with the modulation of micro RNAs (miRNAs), actively released in body fluids and recognized as accurate biomarkers. The aim of this study was to measure serum miRNA profiles in 18 horses taking part in endurance competitions, which represents a good model to test metabolic responses to moderate intensity prolonged efforts. Serum levels of miRNAs of eight horses that were eliminated due to metabolic unbalance (Non Performer-NP) were compared to those of 10 horses that finished an endurance competition in excellent metabolic condition (Performer-P). Circulating miRNA (ci-miRNA) profiles in serum were analyzed through sequencing, and differential gene expression analysis was assessed comparing NP versus P groups. Target and pathway analysis revealed the up regulation of a set of miRNAs (of mir-211 mir-451, mir-106b, mir-15b, mir-101-1, mir-18a, mir-20a) involved in the modulation of myogenesis, cardiac and skeletal muscle remodeling, angiogenesis, ventricular contractility, and in the regulation of gene expression. Our preliminary data open new scenarios in the definition of metabolic adaptations to the establishment of efficient training programs and the validation of athletes' elimination from competitions.
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Publication Date: 2021-12-09 PubMed ID: 34946914PubMed Central: PMC8701225DOI: 10.3390/genes12121965Google Scholar: Lookup
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  • Journal Article
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study investigates how physical exercise influences the activity of micro RNAs (miRNAs) in horses. The researchers found that endurance exercise can alter the miRNAs, which may potentially help in optimizing training programs and determining whether horses should be withdrawn from competitions.

Objectives and Methodology

  • The primary objective of this research was to understand how metabolic imbalances due to long-term exercise could influence the transcriptional profile (miRNAs) of horse athletes.
  • Eighteen horses participating in endurance competitions were observed as part of the study.
  • These horses’ endurance competitions were chosen as the study model as they represent the metabolic responses to prolonged moderately intense efforts.
  • The researchers segregated the horses into two groups. The first group consisted of eight horses that were eliminated due to metabolic imbalance (Non Performer – NP), and the second had ten horses that finished the race in excellent metabolic conditions (Performer-P).
  • The circulating miRNA (ci-miRNA) profiles in the serum of these horses were then analyzed through sequencing.
  • A differential gene expression analysis was carried out by comparing the NP and P groups.

Findings

  • The study identified an upregulation in a set of miRNAs (including mir-211, mir-451, mir-106b, mir-15b, mir-101-1, mir-18a, mir-20a) in the horses.
  • These miRNAs are involved in the modulation of myogenesis (formation of muscle tissue), cardiac and skeletal muscle remodeling, angiogenesis (formation of new blood vessels), ventricular contractility (ability of heart muscle to contract), and the regulation of gene expression.

Implications

  • The findings from this pilot study open up new avenues in understanding the metabolic adaptations that occur due to endurance exercises.
  • These insights could potentially aid in the development of more efficient training programs and can provide significant criteria for deciding on the elimination of athletes from competitions due to metabolic imbalances.

Cite This Article

APA
Cappelli K, Mecocci S, Capomaccio S, Beccati F, Palumbo AR, Tognoloni A, Pepe M, Chiaradia E. (2021). Circulating Transcriptional Profile Modulation in Response to Metabolic Unbalance Due to Long-Term Exercise in Equine Athletes: A Pilot Study. Genes (Basel), 12(12). https://doi.org/10.3390/genes12121965

Publication

Genes
ISSN: 2073-4425
NlmUniqueID: 101551097
Country: Switzerland
Language: English
Volume: 12
Issue: 12

Researcher Affiliations

Cappelli, Katia
  • Department of Veterinary Medicine, University of Perugia, 06126 Perugia, Italy.
  • Sports Horse Research Center, University of Perugia, 06126 Perugia, Italy.
Mecocci, Samanta
  • Department of Veterinary Medicine, University of Perugia, 06126 Perugia, Italy.
Capomaccio, Stefano
  • Department of Veterinary Medicine, University of Perugia, 06126 Perugia, Italy.
  • Sports Horse Research Center, University of Perugia, 06126 Perugia, Italy.
Beccati, Francesca
  • Department of Veterinary Medicine, University of Perugia, 06126 Perugia, Italy.
  • Sports Horse Research Center, University of Perugia, 06126 Perugia, Italy.
Palumbo, Andrea Rosario
  • Department of Veterinary Medicine, University of Perugia, 06126 Perugia, Italy.
Tognoloni, Alessia
  • Department of Veterinary Medicine, University of Perugia, 06126 Perugia, Italy.
Pepe, Marco
  • Department of Veterinary Medicine, University of Perugia, 06126 Perugia, Italy.
  • Sports Horse Research Center, University of Perugia, 06126 Perugia, Italy.
Chiaradia, Elisabetta
  • Department of Veterinary Medicine, University of Perugia, 06126 Perugia, Italy.
  • Sports Horse Research Center, University of Perugia, 06126 Perugia, Italy.

MeSH Terms

  • Animals
  • Biomarkers / metabolism
  • Circulating MicroRNA / genetics
  • Female
  • Gene Expression Regulation
  • Horses / physiology
  • Male
  • Metabolic Diseases / physiopathology
  • Physical Conditioning, Animal
  • Physical Endurance
  • Pilot Projects
  • Transcriptome

Conflict of Interest Statement

The authors declare no conflict of interest. The funders had no role in the design of the study, in the collection, analyses, or interpretation of data, in the writing of the manuscript, or in the decision to publish the results.

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