Comparison of allogeneic platelet lysate and fetal bovine serum for in vitro expansion of equine bone marrow-derived mesenchymal stem cells.
Abstract: Mesenchymal stem cells (MSCs) are promising candidates for cell-based therapy and tissue engineering approaches. Fetal bovine serum (FBS) is commonly used for in vitro MSC expansion; however, the use of FBS may be associated with ethical, scientific, and safety issues. This study aimed to compare the ability of allogeneic platelet lysate (PL) and FBS to cause equine bone marrow-derived MSC expansion. MSCs were isolated from bone marrow aspirate in media supplemented with either PL or FBS, and cell proliferation properties and characteristics were examined. There were no significant differences in MSC yield, colony-forming unit-fibroblast (CFU-F) assay, and population doubling time between PL and FBS cultures. In addition, both PL-MSCs and FBS-MSCs showed similar results in term of ALP staining, osteogenic differentiation, and RT-PCR, although there were subtle differences in morphology, growth pattern, and adhesive properties. These results suggest that PL is a suitable alternative to FBS for use in equine MSC expansion, without the problems related to FBS use.
Published by Elsevier India Pvt Ltd.
Publication Date: 2013-05-16 PubMed ID: 23683731DOI: 10.1016/j.rvsc.2013.04.024Google Scholar: Lookup
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- Journal Article
Summary
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This research explores the comparison between the use of allogeneic platelet lysate (PL) and the traditional use of fetal bovine serum (FBS) in the process of expanding mesenchymal stem cells (MSCs) derived from horse bone marrow. The findings reveal that PL can work as an effective substitute for FBS in this context, thus reducing the drawbacks associated with FBS use.
Research Objectives and Methodology
The main goal of this study was to investigate the capacity of allogeneic platelet lysate (PL) compared to fetal bovine serum (FBS) in expanding equine bone marrow-derived mesenchymal stem cells (MSCs) in vitro.
- Mesenchymal stem cells were isolated from horse bone marrow in medium enriched with either PL or FBS.
- The factors inspected included the proliferation of stem cells and their properties.
- Features studied included MSC yield, colony-forming unit-fibroblast (CFU-F) assay, population doubling time, and comparisons were made between cultures grown with PL and FBS.
Research Findings
The research findings concluded that the use of PL stands on par with FBS when it comes to their effectiveness in mesenchymal stem cell expansion.
- The research found no noteworthy differences in MSC yield, the CFU-F assay, and population doubling time between cultures expanded using PL and FBS.
- Both PL-MSCs and FBS-MSCs demonstrated similar outcomes in ALP staining, osteogenic differentiation, and RT-PCR. There were however visible differences of a minor nature in the morphology, growth pattern, and adhesive properties.
Conclusion and Implications
The results infer that PL is an appropriate alternative to FBS when it comes to expanding horse’s MSCs. The importance of these findings lies in the fact that there are ethical, scientific, and safety concerns associated with FBS use.
- The research explains that by using PL in place of FBS, the moral and scientific challenges tied to FBS use can be circumvented.
- This also implies potential progress in the field of cell-based therapy and tissue engineering approaches as MSCs are promising candidates for such medical practices.
Cite This Article
APA
Seo JP, Tsuzuki N, Haneda S, Yamada K, Furuoka H, Tabata Y, Sasaki N.
(2013).
Comparison of allogeneic platelet lysate and fetal bovine serum for in vitro expansion of equine bone marrow-derived mesenchymal stem cells.
Res Vet Sci, 95(2), 693-698.
https://doi.org/10.1016/j.rvsc.2013.04.024 Publication
Researcher Affiliations
- Department of Clinical Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
MeSH Terms
- Alkaline Phosphatase
- Animals
- Blood Platelets
- Cattle
- Cell Culture Techniques
- Cell Extracts / pharmacology
- Cell Proliferation
- Fetal Blood
- Horses / physiology
- Mesenchymal Stem Cells / cytology
- Mesenchymal Stem Cells / physiology
- Osteoblasts
- Reverse Transcriptase Polymerase Chain Reaction
- Staining and Labeling
Citations
This article has been cited 13 times.- de Kanter AJ, Jongsma KR, Verhaar MC, Bredenoord AL. The Ethical Implications of Tissue Engineering for Regenerative Purposes: A Systematic Review.. Tissue Eng Part B Rev 2023 Apr;29(2):167-187.
- Hagen A, Niebert S, Brandt VP, Holland H, Melzer M, Wehrend A, Burk J. Functional properties of equine adipose-derived mesenchymal stromal cells cultured with equine platelet lysate.. Front Vet Sci 2022;9:890302.
- Pilgrim CR, McCahill KA, Rops JG, Dufour JM, Russell KA, Koch TG. A Review of Fetal Bovine Serum in the Culture of Mesenchymal Stromal Cells and Potential Alternatives for Veterinary Medicine.. Front Vet Sci 2022;9:859025.
- Jeyaraman M, Bingi SK, Muthu S, Jeyaraman N, Packkyarathinam RP, Ranjan R, Sharma S, Jha SK, Khanna M, Rajendran SNS, Rajendran RL, Gangadaran P. Impact of the Process Variables on the Yield of Mesenchymal Stromal Cells from Bone Marrow Aspirate Concentrate.. Bioengineering (Basel) 2022 Jan 29;9(2).
- Hagen A, Holland H, Brandt VP, Doll CU, Häußler TC, Melzer M, Moellerberndt J, Lehmann H, Burk J. Platelet Lysate for Mesenchymal Stromal Cell Culture in the Canine and Equine Species: Analogous but Not the Same.. Animals (Basel) 2022 Jan 13;12(2).
- Hagen A, Lehmann H, Aurich S, Bauer N, Melzer M, Moellerberndt J, Patané V, Schnabel CL, Burk J. Scalable Production of Equine Platelet Lysate for Multipotent Mesenchymal Stromal Cell Culture.. Front Bioeng Biotechnol 2020;8:613621.
- Longhini ALF, Salazar TE, Vieira C, Trinh T, Duan Y, Pay LM, Li Calzi S, Losh M, Johnston NA, Xie H, Kim M, Hunt RJ, Yoder MC, Santoro D, McCarrel TM, Grant MB. Peripheral blood-derived mesenchymal stem cells demonstrate immunomodulatory potential for therapeutic use in horses.. PLoS One 2019;14(3):e0212642.
- Gilbertie JM, Long JM, Schubert AG, Berglund AK, Schaer TP, Schnabel LV. Pooled Platelet-Rich Plasma Lysate Therapy Increases Synoviocyte Proliferation and Hyaluronic Acid Production While Protecting Chondrocytes From Synoviocyte-Derived Inflammatory Mediators.. Front Vet Sci 2018;5:150.
- Naskou MC, Sumner SM, Chocallo A, Kemelmakher H, Thoresen M, Copland I, Galipeau J, Peroni JF. Platelet lysate as a novel serum-free media supplement for the culture of equine bone marrow-derived mesenchymal stem cells.. Stem Cell Res Ther 2018 Mar 22;9(1):75.
- Espina M, Jülke H, Brehm W, Ribitsch I, Winter K, Delling U. Evaluation of transport conditions for autologous bone marrow-derived mesenchymal stromal cells for therapeutic application in horses.. PeerJ 2016;4:e1773.
- Chen X, Wang C, Yin J, Xu J, Wei J, Zhang Y. Efficacy of Mesenchymal Stem Cell Therapy for Steroid-Refractory Acute Graft-Versus-Host Disease following Allogeneic Hematopoietic Stem Cell Transplantation: A Systematic Review and Meta-Analysis.. PLoS One 2015;10(8):e0136991.
- Aguilar E, Bagó JR, Soler-Botija C, Alieva M, Rigola MA, Fuster C, Vila OF, Rubio N, Blanco J. Fast-proliferating adipose tissue mesenchymal-stromal-like cells for therapy.. Stem Cells Dev 2014 Dec 1;23(23):2908-20.
- Tavakolinejad S, Khosravi M, Mashkani B, Ebrahimzadeh Bideskan A, Sanjar Mossavi N, Parizadeh MR, Hamidi Alamdari D. The effect of human platelet-rich plasma on adipose-derived stem cell proliferation and osteogenic differentiation.. Iran Biomed J 2014 Jul;18(3):151-157.
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