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Research in veterinary science2013; 95(2); 693-698; doi: 10.1016/j.rvsc.2013.04.024

Comparison of allogeneic platelet lysate and fetal bovine serum for in vitro expansion of equine bone marrow-derived mesenchymal stem cells.

Abstract: Mesenchymal stem cells (MSCs) are promising candidates for cell-based therapy and tissue engineering approaches. Fetal bovine serum (FBS) is commonly used for in vitro MSC expansion; however, the use of FBS may be associated with ethical, scientific, and safety issues. This study aimed to compare the ability of allogeneic platelet lysate (PL) and FBS to cause equine bone marrow-derived MSC expansion. MSCs were isolated from bone marrow aspirate in media supplemented with either PL or FBS, and cell proliferation properties and characteristics were examined. There were no significant differences in MSC yield, colony-forming unit-fibroblast (CFU-F) assay, and population doubling time between PL and FBS cultures. In addition, both PL-MSCs and FBS-MSCs showed similar results in term of ALP staining, osteogenic differentiation, and RT-PCR, although there were subtle differences in morphology, growth pattern, and adhesive properties. These results suggest that PL is a suitable alternative to FBS for use in equine MSC expansion, without the problems related to FBS use.
Published by Elsevier India Pvt Ltd.
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Publication Date: 2013-05-16 PubMed ID: 23683731DOI: 10.1016/j.rvsc.2013.04.024Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research explores the comparison between the use of allogeneic platelet lysate (PL) and the traditional use of fetal bovine serum (FBS) in the process of expanding mesenchymal stem cells (MSCs) derived from horse bone marrow. The findings reveal that PL can work as an effective substitute for FBS in this context, thus reducing the drawbacks associated with FBS use.

Research Objectives and Methodology

The main goal of this study was to investigate the capacity of allogeneic platelet lysate (PL) compared to fetal bovine serum (FBS) in expanding equine bone marrow-derived mesenchymal stem cells (MSCs) in vitro.

  • Mesenchymal stem cells were isolated from horse bone marrow in medium enriched with either PL or FBS.
  • The factors inspected included the proliferation of stem cells and their properties.
  • Features studied included MSC yield, colony-forming unit-fibroblast (CFU-F) assay, population doubling time, and comparisons were made between cultures grown with PL and FBS.

Research Findings

The research findings concluded that the use of PL stands on par with FBS when it comes to their effectiveness in mesenchymal stem cell expansion.

  • The research found no noteworthy differences in MSC yield, the CFU-F assay, and population doubling time between cultures expanded using PL and FBS.
  • Both PL-MSCs and FBS-MSCs demonstrated similar outcomes in ALP staining, osteogenic differentiation, and RT-PCR. There were however visible differences of a minor nature in the morphology, growth pattern, and adhesive properties.

Conclusion and Implications

The results infer that PL is an appropriate alternative to FBS when it comes to expanding horse’s MSCs. The importance of these findings lies in the fact that there are ethical, scientific, and safety concerns associated with FBS use.

  • The research explains that by using PL in place of FBS, the moral and scientific challenges tied to FBS use can be circumvented.
  • This also implies potential progress in the field of cell-based therapy and tissue engineering approaches as MSCs are promising candidates for such medical practices.

Cite This Article

APA
Seo JP, Tsuzuki N, Haneda S, Yamada K, Furuoka H, Tabata Y, Sasaki N. (2013). Comparison of allogeneic platelet lysate and fetal bovine serum for in vitro expansion of equine bone marrow-derived mesenchymal stem cells. Res Vet Sci, 95(2), 693-698. https://doi.org/10.1016/j.rvsc.2013.04.024

Publication

Research in veterinary science
ISSN: 1532-2661
NlmUniqueID: 0401300
Country: England
Language: English
Volume: 95
Issue: 2
Pages: 693-698
PII: S0034-5288(13)00150-1

Researcher Affiliations

Seo, Jong-pil
  • Department of Clinical Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
Tsuzuki, Nao
    Haneda, Shingo
      Yamada, Kazutaka
        Furuoka, Hidefumi
          Tabata, Yasuhiko
            Sasaki, Naoki

              MeSH Terms

              • Alkaline Phosphatase
              • Animals
              • Blood Platelets
              • Cattle
              • Cell Culture Techniques
              • Cell Extracts / pharmacology
              • Cell Proliferation
              • Fetal Blood
              • Horses / physiology
              • Mesenchymal Stem Cells / cytology
              • Mesenchymal Stem Cells / physiology
              • Osteoblasts
              • Reverse Transcriptase Polymerase Chain Reaction
              • Staining and Labeling

              Citations

              This article has been cited 18 times.
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