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Home / Equine Research Bank / Mycopathologia / Concentrations of fumonisin B1 in feeds associated with anim...
Mycopathologia1991; 114(3); 129-135; doi: 10.1007/BF00437200

Concentrations of fumonisin B1 in feeds associated with animal health problems.

Abstract: Ninety-eight samples of feeds associated with 44 cases of equine leukoencephalomalacia (ELEM) and 83 samples of feed associated with 42 cases of a porcine pulmonary edema syndrome (PPE) were analyzed for fumonisin B1 (FB1). For comparison purposes, 51 feed samples not associated with PPE or ELEM were also analyzed. Feed associated with ELEM contained FB1 ranging from less than 1 microgram/g to 126 micrograms/g with 75% of the cases having at least 1 sample above 10 micrograms/g. Feeds associated with PPE ranged from less than 1 microgram/g to 330 micrograms/g with 71% of the cases having at least 1 sample greater than 10 micrograms/g. Quantitation was by high performance liquid chromatography (HPLC)/fluorescence using the fluorescamine derivative with confirmation by thin layer chromatography (TLC) and/or gas chromatography/mass spectroscopy (GC/MS).
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Publication Date: 1991-06-01 PubMed ID: 1886638DOI: 10.1007/BF00437200Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper is about a study that examined the presence of a toxin, fumonisin B1 (FB1), in feed associated with two animal diseases – equine leukoencephalomalacia (ELEM) and porcine pulmonary edema syndrome (PPE).

Objective and Sample Size

  • The objective of the study was to determine the concentration of a toxin called fumonisin B1 (FB1) in feeds that were associated with instances of two health problems in animals – equine leukoencephalomalacia (ELEM) in horses and porcine pulmonary edema syndrome (PPE) in swine.
  • A total of 232 feed samples were analyzed in this research. Ninety-eight of them were taken from feeds linked to cases of ELEM, 83 samples were associated with instances of PPE, and for comparison purposes, 51 feed samples not linked to either PPE or ELEM were also examined.

Fumonisin B1 Concentrations in the Feed Samples

  • In the case of ELEM, the concentration of FB1 in the examined feed samples ranged from less than 1 microgram per gram to 126 micrograms per gram.
  • The feed samples associated with 75% of the ELEM cases had at least one sample that had an FB1 concentration above 10 micrograms per gram.
  • The feed samples linked with PPE had FB1 levels that ranged from less than 1 microgram per gram to as high as 330 micrograms per gram.
  • Around 71% of the feed samples associated with PPE had at least one sample with FB1 concentration that exceeded 10 micrograms per gram.

Methodology

  • The quantification of FB1 levels in the feed samples was conducted using high performance liquid chromatography (HPLC) with fluorescence. HPLC is a method used in analytical chemistry to separate, identify, and quantify each component in a mixture.
  • The researchers used fluorescamine derivative in their HPLC process. Fluorescamine is a chemical that is commonly used for detecting primary amines.
  • The researchers further confirmed their findings through the use of thin layer chromatography (TLC) and/or gas chromatography/mass spectroscopy (GC/MS). These are other techniques used in chemistry to analyze and identify substances within a test sample.

Cite This Article

APA
Ross PF, Rice LG, Plattner RD, Osweiler GD, Wilson TM, Owens DL, Nelson HA, Richard JL. (1991). Concentrations of fumonisin B1 in feeds associated with animal health problems. Mycopathologia, 114(3), 129-135. https://doi.org/10.1007/BF00437200

Publication

Mycopathologia
ISSN: 0301-486X
NlmUniqueID: 7505689
Country: Netherlands
Language: English
Volume: 114
Issue: 3
Pages: 129-135

Researcher Affiliations

Ross, P F
  • US Department of Agriculture, Animal and Plant Health Inspection Service, National Veterinary Services Laboratories, Ames, IA 50010.
Rice, L G
    Plattner, R D
      Osweiler, G D
        Wilson, T M
          Owens, D L
            Nelson, H A
              Richard, J L

                MeSH Terms

                • Animal Feed / adverse effects
                • Animal Feed / analysis
                • Animals
                • Carcinogens, Environmental / adverse effects
                • Carcinogens, Environmental / analysis
                • Chromatography, High Pressure Liquid
                • Chromatography, Thin Layer
                • Encephalomalacia / etiology
                • Encephalomalacia / veterinary
                • Food Microbiology
                • Fumonisins
                • Gas Chromatography-Mass Spectrometry
                • Horse Diseases / etiology
                • Horses
                • Mycotoxins / adverse effects
                • Mycotoxins / analysis
                • Pulmonary Edema / etiology
                • Pulmonary Edema / veterinary
                • Swine
                • Swine Diseases / etiology
                • Zea mays

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