Determination of leucine enkephalin and methionine enkephalin in equine cerebrospinal fluid by microbore high-performance liquid chromatography and capillary zone electrophoresis coupled to tandem mass spectrometry.
Abstract: The performance of microbore high-performance liquid chromatography and capillary zone electrophoresis, both equipped with on-line tandem mass spectrometric detection capability, was evaluated critically for the determination of endogenous amounts of leucine enkephalin and methionine enkephalin in equine cerebrospinal fluid. Using an identical sample clean-up and enrichment procedure, capillary zone electrophoresis-mass spectrometry is limited in its concentration detection capacity owing to its much smaller injection volume. Leucine enkephalin was identified in post-mortem equine cerebrospinal fluid at the 1-5 ng/ml level by liquid chromatography-mass spectrometry.
Publication Date: 1989-10-27 PubMed ID: 2613826DOI: 10.1016/s0378-4347(00)82608-0Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research article is about the evaluation of using microbore high-performance liquid chromatography and capillary zone electrophoresis with tandem mass spectrometer to determine the presence of leucine enkephalin and methionine enkephalin in horse cerebrospinal fluid.
Objective of the research
- The main aim of this research was to evaluate the efficiency of microbore high-performance liquid chromatography (HPLC) and capillary zone electrophoresis (CZE), when coupled with on-line tandem mass spectrometry, in determining the presence and amount of both leucine enkephalin and methionine enkephalin in equine cerebrospinal fluid.
Methods used in the research
- Both CZE and HPLC techniques, paired with on-line tandem mass spectrometric detection, were employed in this research. These chromatographic methods are often used for separating mixtures of substances for analysis, and here they were used to detect and separate the leucine enkephalin and methionine enkephalin in the cerebrospinal fluid.
- The same sample clean-up and enrichment procedure was used for both methods, to allow for a fair comparison of their effectiveness.
Findings of the research
- The results showed that CZE-MS was limited in its concentration detection capacity due to its much smaller injection volume compared to HPLC-MS.
- HPLC-MS successfully identified the presence of leucine enkephalin in post-mortem equine cerebrospinal fluid at levels between 1-5 ng/ml, proving its effectiveness in detecting endogenous compounds at lower concentrations.
Implications of the research
- The findings suggest that HPLC-MS is more effective in tracing endogenous compounds in cerebrospinal fluid, as it can detect lower concentrations of these substances compared to CZE-MS.
- This research offers invaluable insights into the study of neurochemicals in equine cerebrospinal fluid.
Cite This Article
APA
Mück WM, Henion JD.
(1989).
Determination of leucine enkephalin and methionine enkephalin in equine cerebrospinal fluid by microbore high-performance liquid chromatography and capillary zone electrophoresis coupled to tandem mass spectrometry.
J Chromatogr, 495, 41-59.
https://doi.org/10.1016/s0378-4347(00)82608-0 Publication
Researcher Affiliations
- Equine Drug Testing and Toxicology Program, New York State College of Veterinary Medicine, Cornell University, Ithaca 14850.
MeSH Terms
- Animals
- Chromatography, High Pressure Liquid
- Electrophoresis / methods
- Enkephalin, Leucine / cerebrospinal fluid
- Enkephalin, Methionine / cerebrospinal fluid
- Horses
- Mass Spectrometry
Citations
This article has been cited 2 times.- Perkins JR, Parker CE, Tomer KB. Nanoscale separations combined with electrospray ionization mass spectrometry: Sulfonamide determination. J Am Soc Mass Spectrom 1992 Feb;3(2):139-49.
- Dass C, Kusmierz JJ, Desiderio DM, Jarvis SA, Green BN. Electrospray mass spectrometry for the analysis of opioid peptides and for the quantification of endogenous methionine enkephalin and β-endorphin. J Am Soc Mass Spectrom 1991 Apr;2(2):149-56.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
