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Home / Equine Research Bank / J Virol Methods / Development and evaluation of an ELISA using recombinant fus...
Journal of virological methods1995; 54(1); 1-13; doi: 10.1016/0166-0934(95)00020-u

Development and evaluation of an ELISA using recombinant fusion protein to detect the presence of host antibody to equine arteritis virus.

Abstract: A recombinant glutathione-S-transferase fusion protein expressing amino acids 55-98 of equine arteritis virus (EAV) GL (rGL 55-98) was tested in an ELISA for its ability to detect serum antibodies to EAV. Host antibodies induced following EAV infection bound the recombinant antigen by ELISA. The ELISA specificity and sensitivity were determined with a panel of equine sera including postinfection and postvaccination samples. A good correlation existed between EAV neutralizing antibody titers and ELISA absorbance values (r = 0.827). The sensitivity and specificity of the ELISA were 99.6 and 90.1%, respectively, compared with EAV neutralization test and the recombinant antigen did not crossreact in ELISA with equine sera directed against other common equine respiratory viruses. Three post-EAV infection equine sera raised against different EAV isolates reacted strongly in the ELISA, as did two equine sera raised against EAV vaccines, indicating that the viral epitope was conserved between the viruses tested. Following vaccination with an inactivated whole virus vaccine, antibody detected with the recombinant antigen ELISA preceded the development of a virus-neutralizing response. The study demonstrates the potential application of rGL 55-98 as a diagnostic antigen.
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Publication Date: 1995-07-01 PubMed ID: 7559853PubMed Central: PMC7119792DOI: 10.1016/0166-0934(95)00020-uGoogle Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article is about the development of an ELISA test using a recombinant fusion protein to identify the presence of antibodies against the equine arteritis virus.

Understanding the Research

  • The research paper primarily focuses on the development and evaluation of a specific ELISA (Enzyme-Linked Immunosorbent Assay) which employs a recombinant fusion protein. This protein expressed certain amino acids (specifically 55-98) from the equine arteritis virus’ (EAV) GL (generally a term used for glycoprotein).
  • The goal was to detect the occurrence of serum antibodies to EAV which might have been induced following an infection. Essentially, the researchers wanted to devise a way to detect if a horse had been previously infected with the virus.
  • The sensitivity and specificity of the newly developed ELISA test were a chief concern in the study. A thorough comparison was done with samples of equine serum, including those taken after the infection and after vaccination.

Testing and Results

  • A strong correlation was observed between EAV neutralizing antibody titers (a measure of how much antibody an organism has produced) and the absorbance values obtained from the ELISA.
  • The sensitivity (probability that the test can correctly identify those with the disease) and specificity (probability that the test will correctly identify those without the disease) of the ELISA were 99.6 and 90.1%, respectively. These were gauged in comparison with an EAV neutralization test.
  • An important revelation from the study was that the recombinant antigen did not cross-react with equine sera which were directed against other common equine respiratory viruses. This implies that the ELISA test developed was specific to the EAV.
  • The epitope (a specific portion of an antigen to which an antibody binds) was found to be conserved across different EAV isolates, as strong reactions were detected in the ELISA in equine sera raised against different EAV isolates.

Implications

  • In the case of horses that were vaccinated with an inactivated whole virus vaccine, it was observed that the antibody detected via the recombinant antigen ELISA preceded the development of a virus-neutralizing response. This could make the new ELISA test a potential tool for early detection and prevention of the disease.
  • The new recombinant antigen ELISA demonstrated potential for application as a diagnostic antigen, offering a more efficient, specific and sensitive method of detecting antibodies to EAV.

Cite This Article

APA
Chirnside ED, Francis PM, de Vries AA, Sinclair R, Mumford JA. (1995). Development and evaluation of an ELISA using recombinant fusion protein to detect the presence of host antibody to equine arteritis virus. J Virol Methods, 54(1), 1-13. https://doi.org/10.1016/0166-0934(95)00020-u

Publication

Journal of virological methods
ISSN: 0166-0934
NlmUniqueID: 8005839
Country: Netherlands
Language: English
Volume: 54
Issue: 1
Pages: 1-13

Researcher Affiliations

Chirnside, E D
  • Department of Infectious Diseases, Animal Health Trust, Lanwades Park, Kennett, Newmarket, Suffolk, UK.
Francis, P M
    de Vries, A A
      Sinclair, R
        Mumford, J A

          MeSH Terms

          • Animals
          • Antibodies, Viral / analysis
          • Cell Line
          • Enzyme-Linked Immunosorbent Assay / methods
          • Enzyme-Linked Immunosorbent Assay / veterinary
          • Equartevirus / genetics
          • Equartevirus / immunology
          • Evaluation Studies as Topic
          • Horses
          • Membrane Glycoproteins / genetics
          • Membrane Glycoproteins / immunology
          • Recombinant Fusion Proteins / immunology
          • Sensitivity and Specificity
          • Viral Envelope Proteins / genetics
          • Viral Envelope Proteins / immunology

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          Citations

          This article has been cited 14 times.
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