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Home / Equine Research Bank / Ir Vet J / Dietary supplementation of new-born foals with free nucleoti...
Irish veterinary journal2025; 78(1); 7; doi: 10.1186/s13620-025-00294-3

Dietary supplementation of new-born foals with free nucleotides positively affects neonatal diarrhoea management.

Abstract: Foals commonly experience diarrhoea in the first weeks of life. Although this condition is rarely life-threatening, it can have significant health consequences. This study investigated whether new-born foals can benefit from a dietary supplement of nucleotides, as already demonstrated in other species. Dietary nucleotides have positive effects on rapidly proliferating tissues and are considered "semi-essential nutrients" since cells have only a limited capacity to synthesize these compounds. The aim of this study was to investigate whether providing foals with a dietary nucleotide supplementation, in the form of an oral paste, was able to affect diarrhoea incidence, systemic immunity, intestinal microbiota and volatile fatty acid production. Thirty new-born standardbred foals, from 3 different premises within the same area, were equally distributed between two groups: one group received an oral paste containing dietary nucleotides (NUCL group), while the other received a placebo paste (CTRL group). Faecal and blood samples were collected on days 1 and 35 after birth. No statistical differences in cytokines (TNF-α, IFN-γ, IL-6, IL-12) or faecal calprotectin levels were found between the two groups, suggesting that the level of nucleotide supplementation used in this study did not have significant effects on the systemic immune system and on the levels of faecal calprotectin. However, the NUCL group showed a lower relative frequency of number of days with diarrhoea (6.12% vs 13.33%; p < 0.001) and greater weight gain compared with the CTRL group (50.3 ± 5.65 kg vs 44.0 ± 8.65 kg; p < 0.05). Total volatile fatty acids, branched volatile fatty acids, acetic acid, propionic acid, butyric acid, succinic acid and iso-butyric acids in faecal samples were all higher in the NUCL group compared with the CTRL group. This outcome may explain an earlier establishment of a gut microbiota in the foals of the NUCL group that was closer to that typical of an adult horse, characterised by predominant fibrolytic populations. Volatile fatty acid production (especially butyric acid) has also been shown to correlate with the intestinal well-being of the horse, supporting the use of dietary nucleotide supplements for improved health and well-being in new-born foals. Although we noted no clear differences in the faecal microbial communities between the two groups, dietary nucleotide supplementation did appear to have a positive clinical outcome, reducing the number of days of diarrhoea and increasing the levels of volatile fatty acids.
© 2025. The Author(s).
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Publication Date: 2025-03-01 PubMed ID: 40025599PubMed Central: PMC11871744DOI: 10.1186/s13620-025-00294-3Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study explores the impact of dietary nucleotide supplementation in newborn foals’ diet and its effect on their rate of diarrhoea incidence, overall weight gain, and gut health. The research revealed that foals supplemented with nucleotides showed a lower rate of diarrhoea and more impressive weight gain when compared to those on a placebo.

Methodology

  • The study involved 30 newborn standardbred foals from 3 different premises within the same area. They were equally split into two groups: The NUCL group received a dietary nucleotide oral supplement, and the CTRL group received a placebo paste.
  • Blood and fecal samples were gathered on days 1 and 35 post-birth for analysis.

Results

  • There was no significant difference in cytokine levels (TNF-α, IFN-γ, IL-6, IL-12) or fecal calprotectin levels between the two groups, indicating that the nucleotide supplementation didn’t greatly affect the systemic immune system or fecal calprotectin levels.
  • The group supplemented with nucleotides demonstrated a lower relative frequency of days with diarrhoea (6.12% vs 13.33%; p < 0.001) and showed a higher weight gain compared to the control group (50.3 ± 5.65 kg versus 44.0 ± 8.65 kg; p < 0.05).
  • In terms of gut health, there were higher counts of total volatile fatty acids, branched volatile fatty acids, acetic acid, propionic acid, butyric acid, succinic acid, and iso-butyric acids in the feces of the NUCL group compared to the CTRL group. This result points to an early establishment of a gut microbiota in the nucleotide-supplemented foals that aligned closer to that seen in an adult horse.

Implications

  • The results suggest that dietary nucleotide supplementation could have a positive impact on newborn foals’ health, reducing the occurrence of diarrhoea and increasing weight gain.
  • It also shows potential benefits regarding the gut microbiota establishment, highlighting the role of dietary nucleotide supplementation in enhancing digestive health and overall well-being in newborn foals.
  • However, more significant differences in the microbial communities between the two groups were not noted, suggesting the need for further investigation.

Cite This Article

APA
Penazzi L, Pagliara E, Nervo T, Ala U, Bertuglia A, Romano G, Hattab J, Tiscar PG, Bergagna S, Pagliasso G, Antoniazzi S, Cavallarin L, Valle E, Prola L. (2025). Dietary supplementation of new-born foals with free nucleotides positively affects neonatal diarrhoea management. Ir Vet J, 78(1), 7. https://doi.org/10.1186/s13620-025-00294-3

Publication

Irish veterinary journal
ISSN: 2046-0481
NlmUniqueID: 0100762
Country: Ireland
Language: English
Volume: 78
Issue: 1
Pages: 7
PII: 7

Researcher Affiliations

Penazzi, Livio
  • Department of Veterinary Sciences, University of Turin, Largo Braccini 2, Grugliasco, 10095, Italy. livio.penazzi@unito.it.
Pagliara, Eleonora
  • Department of Veterinary Sciences, University of Turin, Largo Braccini 2, Grugliasco, 10095, Italy.
Nervo, Tiziana
  • Department of Veterinary Sciences, University of Turin, Largo Braccini 2, Grugliasco, 10095, Italy.
Ala, Ugo
  • Department of Veterinary Sciences, University of Turin, Largo Braccini 2, Grugliasco, 10095, Italy.
Bertuglia, Andrea
  • Department of Veterinary Sciences, University of Turin, Largo Braccini 2, Grugliasco, 10095, Italy.
Romano, Giovanna
  • Centro Equino Arcadia, Frazione Mottura 106, Villafranca Piemonte, 10068, Italy.
Hattab, Jasmine
  • Department of Veterinary Medicine, University of Teramo, SP18 Piano d'Accio, Teramo, 64100, Italy.
Tiscar, Pietro Giorgio
  • Department of Veterinary Medicine, University of Teramo, SP18 Piano d'Accio, Teramo, 64100, Italy.
Bergagna, Stefania
  • Istituto Zooprofilattico Sperimentale Piemonte, Liguria E Valle d'Aosta, Via Bologna 148, Turin, 10154, Italy.
Pagliasso, Giulia
  • Istituto Zooprofilattico Sperimentale Piemonte, Liguria E Valle d'Aosta, Via Bologna 148, Turin, 10154, Italy.
Antoniazzi, Sara
  • Consiglio Nazionale Delle Ricerche, Largo Braccini 2, Grugliasco, 10095, Italy.
Cavallarin, Laura
  • Consiglio Nazionale Delle Ricerche, Largo Braccini 2, Grugliasco, 10095, Italy.
Valle, Emanuela
  • Department of Veterinary Sciences, University of Turin, Largo Braccini 2, Grugliasco, 10095, Italy.
Prola, Liviana
  • Department of Veterinary Sciences, University of Turin, Largo Braccini 2, Grugliasco, 10095, Italy.

Conflict of Interest Statement

Declarations. Ethics approval and consent to participate: The study was approved by the Ethics Committee of the University of Turin (Ethical approval prot. n. 567, University of Turin). The owner of each breeding centre provided their written informed consent to participation to the study. Consent for publication: Not applicable. Competing interests: The authors declare that they have no competing interests.

References

This article includes 92 references
  1. Schoster A, Staempfli HR, Guardabassi LG, Jalali M, Weese JS. Comparison of the fecal bacterial microbiota of healthy and diarrheic foals at two and four weeks of life.. BMC Vet Res 2017;13:144.
    pmc: PMC5450145pubmed: 28558788
  2. Oliver-Espinosa O. Foal diarrhea.. Vet Clinics of North America: Equine Practice 2018;34:55–68.
    pmc: PMC7134762pubmed: 29395727
  3. Magdesian KG. Neonatal foal diarrhea.. Veterinary Clinics of North America: Equine Practice 2005;21:295–312.
    pmc: PMC7135406pubmed: 16051051
  4. Mallicote M, House AM, Sanchez LC. A review of foal diarrhoea from birth to weaning.. Equine Veterinary Education 2012;24:206–14.
    pmc: PMC7163619pubmed: 32313387
  5. Kuhl J, Winterhoff N, Wulf M, Schweigert FJ, Schwendenwein I, Bruckmaier RM. Changes in faecal bacteria and metabolic parameters in foals during the first six weeks of life.. Vet Microbiol 2011;151:321–8.
    pubmed: 21511405
  6. Johnston RH, Kamstra LD, Kohler PH. Mares’ milk composition as related to “foal heat” scours.. J Anim Sci 1970;31:549–53.
    pubmed: 5535335
  7. Masri MD, Merritt AM, Gronwall R, Burrows CF. Faecal composition in foal heat diarrhoea.. Equine Vet J 1986;18:301–6.
    pubmed: 3758010
  8. Costa MC, Weese JS. Understanding the intestinal microbiome in health and disease.. Veterinary Clinics: Equine Practice 2018;34:1–12.
    pubmed: 29402480
  9. John J, Roediger K, Schroedl W, Aldaher N, Vervuert I. Development of intestinal microflora and occurrence of diarrhoea in sucking foals: effects of Bacillus cereus var. toyoi supplementation.. BMC Vet Res 2015;11:34.
    pmc: PMC4333172pubmed: 25889817
  10. De La Torre U, Henderson JD, Furtado KL, Pedroja M, Elenamarie O, Mora A. Utilizing the fecal microbiota to understand foal gut transitions from birth to weaning.. PLoS One 2019;14: e0216211.
    pmc: PMC6490953pubmed: 31039168
  11. Goodman-Davis R, Figurska M, Cywinska A. Gut microbiota manipulation in foals—naturopathic diarrhea management, or unsubstantiated folly?. Pathogens 2021;10: 1137.
    pmc: PMC8467620pubmed: 34578169
  12. Yuyama T, Yusa S, Takai S, Tsubaki S, Kado Y, Morotomi M. Evaluation of a host-specific Lactobacillus Probiotic in neonatal foals.. Int J Appl Res Vet Med 2004;2:26–33.
  13. Sadet-Bourgeteau S, Julliand V. Equine microbial gastro-intestinal health.. EAAP Publications 2010;p. 161–82.
  14. Ströbel C, Günther E, Romanowski K, Büsing K, Urubschurov V, Zeyner A. Effects of oral supplementation of probiotic strains of Lactobacillus rhamnosus and Enterococcus faecium on diarrhoea events of foals in their first weeks of life.. J Anim Physiol Anim Nutr 2018;102:1357–65.
    pubmed: 29790614
  15. Urubschurov V, Stroebel C, Günther E, Romanowski K, Büsing K, Zeyner A. Effect of oral supplementation of probiotic strains of Lactobacillus rhamnosus and Enterococcus faecium on the composition of the faecal microbiota of foals.. J Anim Physiol Anim Nutr 2019;103:915–24.
    pubmed: 30854744
  16. Weese JS, Rousseau J. Evaluation of Lactobacillus pentosus WE7 for prevention of diarrhea in neonatal foals.. J Am Vet Med Assoc 2005;226:2031–4.
    pubmed: 15989186
  17. Schoster A, Staempfli HR, Abrahams M, Jalali M, Weese JS, Guardabassi L. Effect of a probiotic on prevention of diarrhea and Clostridium difficile and Clostridium perfringens shedding in foals.. J Vet Intern Med 2015;29:925–31.
    pmc: PMC4895414pubmed: 25903509
  18. Schoster A, Guardabassi L, Staempfli HR, Abrahams M, Jalali M, Weese JS. The longitudinal effect of a multi-strain probiotic on the intestinal bacterial microbiota of neonatal foals.. Equine Vet J 2016;48:689–96.
    pubmed: 26509834
  19. Costa MC, Stämpfli HR, Allen-Vercoe E, Weese JS. Development of the faecal microbiota in foals.. Equine Vet J 2016;48:681–8.
    pubmed: 26518456
  20. Lindenberg F, Krych L, Kot W, Fielden J, Frøkiær H, van Galen G. Development of the equine gut microbiota.. Sci Rep 2019;9:14427.
    pmc: PMC6783416pubmed: 31594971
  21. Garber A, Hastie P, Murray J-A. Factors influencing equine gut microbiota: current knowledge.. J Equine Vet 2020;88: 102943.
    pubmed: 32303307
  22. Gil A. Modulation of the immune response mediated by dietary nucleotides.. Eur J Clin Nutr 2002;56:S1–4.
    pubmed: 12142952
  23. Ding T, Song G, Liu X, Xu M, Li Y. Nucleotides as optimal candidates for essential nutrients in living organisms: a review.. Journal of Functional Foods 2021;82: 104498.
  24. Qu Z, Tian P, Zhao J, Wang G, Chen W. Feeding the microbiota–gut–brain axis: nucleotides and their role in early life.. Food Frontiers 2023;4:1164–78.
  25. Carver JD, Allan WW. The role of nucleotides in human nutrition.. J Nutr Biochem 1995;6:58–72.
  26. Fontana L, Martínez-Augustin O, Gil Á. Role of dietary nucleotides in immunity.. Functional Food Reviews 2010;2:91–100.
  27. Carver JD. Dietary nucleotides: effects on the immune and gastrointestinal systems.. Acta Paediatr 1999;88:83–8.
    pubmed: 10569229
  28. Oftedal OT, Hintz HF, Schryver HF. Lactation in the horse: milk composition and intake by foals.. J Nutr 1983;113:2096–106.
    pubmed: 6619986
  29. Acworth NRJ. The healthy neonatal foal: routine examinations and preventative medicine.. Equine Veterinary Education 2003;15:207–11.
  30. Knottenbelt DC, Holdstock N, Madigan JE. Equine neonatal medicine and surgery: medicine and surgery.. Amsterdam: Elsevier Health Sciences; 2004.
  31. Bonelli F, Nocera I, Conte G, Panzani D, Sgorbini M. Relation between Apgar scoring and physical parameters in 44 newborn Amiata donkey foals at birth.. Theriogenology 2020;142:310–4.
    pubmed: 31711687
  32. Alonso MA, Boakari YL, Riccio AV, Belli CB, Fernandes CB. Perinatal parameters of mule and equine foals: similarities and differences.. Journal of Veterinary Behavior 2023;63:31–5.
  33. Bolyen E, Rideout JR, Dillon MR, Bokulich NA, Abnet CC, Al-Ghalith GA. Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2.. Nat Biotechnol 2019;37:852–7.
    pmc: PMC7015180pubmed: 31341288
  34. Callahan BJ, McMurdie PJ, Rosen MJ, Han AW, Johnson AJA, Holmes SP. DADA2: high-resolution sample inference from Illumina amplicon data.. Nat Methods 2016;13:581–3.
    pmc: PMC4927377pubmed: 27214047
  35. Katoh K, Misawa K, Kuma K, Miyata T. MAFFT: a novel method for rapid multiple sequence alignment based on fast fourier transform.. Nucleic Acids Res 2002;30:3059–66.
    pmc: PMC135756pubmed: 12136088
  36. Price MN, Dehal PS, Arkin AP. FastTree 2 – approximately maximum-likelihood trees for large alignments.. PLoS One 2010;5:e9490.
    pmc: PMC2835736pubmed: 20224823
  37. Pruesse E, Quast C, Knittel K, Fuchs BM, Ludwig W, Peplies J. SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB.. Nucleic Acids Res 2007;35:7188–96.
    pmc: PMC2175337pubmed: 17947321
  38. Quast C, Pruesse E, Yilmaz P, Gerken J, Schweer T, Yarza P. The SILVA ribosomal RNA gene database project: improved data processing and web-based tools.. Nucleic Acids Res 2013;41:D590–6.
    pmc: PMC3531112pubmed: 23193283
  39. Guantario B, Giribaldi M, Devirgiliis C, Finamore A, Colombino E, Capucchio MT. A comprehensive evaluation of the impact of bovine milk containing different beta-casein profiles on gut health of ageing mice.. Nutrients 2020;12: 2147.
    pmc: PMC7400800pubmed: 32707687
  40. R Core Team. R: a language and environment for statistical computing.. Vienna: R Foundation for Statistical Computing; 2021.
  41. Faith DP. Conservation evaluation and phylogenetic diversity.. Biol Cons 1992;61:1–10.
  42. Pielou EC. The measurement of diversity in different types of biological collections.. J Theor Biol 1966;13:131–44.
  43. Chao A, Chiu CH. Species estimation and applications.. Wiley StatsRef: Statistics Reference Online 2016.
    doi: 10.1002/9781118445112.stat03432.pub2google scholar: lookup
  44. Shannon CE. A mathematical theory of communication.. The Bell System Technical Journal 1948;27:379–423.
  45. Simpson EH. Measurement of diversity.. Nature 1949;163:688–688.
  46. Lozupone CA, Hamady M, Kelley ST, Knight R. Quantitative and qualitative β diversity measures lead to different insights into factors that structure microbial communities.. Appl Environ Microbiol 2007;73:1576–85.
    pmc: PMC1828774pubmed: 17220268
  47. Lozupone C, Knight R. UniFrac: a new phylogenetic method for comparing microbial communities.. Appl Environ Microbiol 2005;71:8228–35.
    pmc: PMC1317376pubmed: 16332807
  48. Bray JR, Curtis JT. An ordination of the upland forest communities of southern Wisconsin.. Ecol Monogr 1957;27:326–49.
  49. Jaccard P. The distribution of the flora in the alpine zone. 1.. New Phytologist 1912;11:37–50.
  50. Anderson MJ. Permutational multivariate analysis of variance (PERMANOVA).. Wiley StatsRef: Statistics Reference Online 2017;p. 1–15.
    doi: 10.1002/9781118445112.stat07841google scholar: lookup
  51. Bauer JE, Harvey JW, Asquith RL, McNulty PK, Kivipelto J. Serum protein reference values in foals during the first year of life: comparison of chemical and electrophoretic methods.. Veterinary Clinical Pathology 1985;14:14–22.
    pubmed: 15221688
  52. Gueimonde M, Sánchez B, de los Reyes-Gavilán C, Margolles A. Antibiotic resistance in probiotic bacteria.. Front Microbiol 2013;4.
    doi: 10.3389/fmicb.2013.00202pmc: PMC3714544pubmed: 23882264google scholar: lookup
  53. Wohlfender FD, Barrelet FE, Doherr MG, Straub R, Meier HP. Diseases in neonatal foals. Part 1: the 30 day incidence of disease and the effect of prophylactic antimicrobial drug treatment during the first three days post partum.. Equine Vet J 2009;41:179–85.
    pubmed: 19418748
  54. Schoster A. Probiotic use in equine gastrointestinal disease.. Vet Clin North Am Equine Pract 2018;34:13–24.
    pubmed: 29402478
  55. Pickering LK, Granoff DM, Erickson JR, Masor ML, Cordle CT, Schaller JP. Modulation of the immune system by human milk and infant formula containing nucleotides.. Pediatrics 1998;101:242–9.
    pubmed: 9445498
  56. Carver JD, Pimentel B, Cox WI, Barness LA. Dietary nucleotide effects upon immune function in infants.. Pediatrics 1991;88:359–63.
    pubmed: 1861940
  57. Sanderson IR, He Y. Nucleotide uptake and metabolism by intestinal epithelial cells.. J Nutr 1994;124:131S–137S.
    pubmed: 8283303
  58. Savaiano DA, Clifford AJ. Adenine, the precursor of nucleic acids in intestinal cells unable to synthesize purines de novo.. J Nutr 1981;111:1816–22.
    pubmed: 7288504
  59. LeLeik NS, Bronstein AD, Baliga BS, Munro HN. De novo purine nucleotide synthesis in the rat small and large intestine: effect of dietary protein and purines.. J Pediatr Gastroenterol Nutr 1983;2:313–9.
    pubmed: 6192234
  60. Cosgrove M. Nucleotides.. Nutrition 1998;14:748–51.
    pubmed: 9785353
  61. Uauy R, Stringel G, Thomas R, Quan R. Effect of dietary nucleosides on growth and maturation of the developing gut in the rat.. J Pediatr Gastroenterol Nutr 1990;10:497–503.
    pubmed: 2358983
  62. Van Buren CT, Rudolph F. Dietary nucleotides: a conditional requirement : is nucleic acid actually a nutrient?. Nutrition 1997;13:470–2.
    pubmed: 9225342
  63. Kulkarni AD, Rudolph FB, Van Buren CT. The role of dietary sources of nucleotides in immune function: a review.. J Nutr 1994;124:1442S–1446S.
    pubmed: 8064400
  64. Carver JD, Cox WI, Barness LA. Dietary nucleotide effects upon murine natural killer cell activity and macrophage activation.. J Parenter Enter Nutr 1990;14:18–22.
    pubmed: 2325242
  65. Yamauchi K, Adjei AA, Ameho CK, Chan YC, Kulkarni AD, Sato S. A nucleoside-nucleotide mixture and its components increase lymphoproliferative and delayed hypersensitivity responses in mice.. J Nutr 1996;126:1571–7.
    pubmed: 8648430
  66. Nagafuchi S, Katayanagi T, Nakagawa E, Takahashi T, Yajima T, Yonekubo A. Effects of dietary nucleotides on serum antibody and splenic cytokine production in mice.. Nutr Res 1997;17:1163–74.
  67. Nagafuchi S, Totsuka M, Hachimura S, Goto M, Takahashi T, Yajima T. Dietary nucleotides increase the proportion of a TCRγδ+ subset of intraepithelial lymphocytes (IEL) and IL-7 production by intestinal epithelial cells (IEC); implications for modification of cellular and molecular cross-talk between IEL and IEC by dietary nucleotides.. Biosci Biotechnol Biochem 2000;64:1459–65.
    pubmed: 10945264
  68. Nagafuchi S, Hachimura S, Totsuka M, Takahashi T, Yajima T. Dietary nucleotides can up-regulate antigen-specific Th1 immune responses and suppress antigen-specific IgE responses in mice.. Int Arch Allergy Immunol 2000;122:33–41.
    pubmed: 10859467
  69. Nagafuchi S, Totsuka M, Hachimura S, Goto M, Takahashi T, Yajima T. Dietary nucleotides increase the mucosal IgA response and the secretion of transforming growth factor β from intestinal epithelial cells in mice.. Cytotechnology 2002;40:49–58.
    pmc: PMC3449534pubmed: 19003104
  70. Jyonouchi H, Sun S, Abiru T, Winship T, Kuchan MJ. Dietary nucleotides modulate antigen-specific type 1 and type 2 t-cell responses in young c57bl/6 mice.. Nutrition 2000;16:442–6.
    pubmed: 10869901
  71. Jyonouchi H, Sun S, Winship T, Kuchan MJ. Dietary ribonucleotides modulate type 1 and type 2 T-helper cell responses against ovalbumin in young BALB/cJ mice.. J Nutr 2001;131:1165–70.
    pubmed: 11285320
  72. Buck RH, Thomas DL, Winship TR, Cordle CT, Kuchan MJ, Baggs GE. Effect of dietary ribonucleotides on infant immune status. Part 2: immune cell development.. Pediatr Res 2004;56:891–900.
    pubmed: 15496603
  73. Schaller JP, Kuchan MJ, Thomas DL, Cordle CT, Winship TR, Buck RH. Effect of dietary ribonucleotides on infant immune status. Part 1: humoral responses.. Pediatr Res 2004;56:883–90.
    pubmed: 15496604
  74. Ostrom KM, Cordle CT, Schaller JP, Winship TR, Thomas DJ, Jacobs JR. Immune status of infants fed soy-based formulas with or without added nucleotides for 1 year: part 1: vaccine responses, and morbidity.. J Pediatr Gastroenterol Nutr 2002;34:137.
    pubmed: 11840030
  75. Lee DN, Liu SR, Chen YT, Wang RC, Lin SY, Weng CF. Effects of diets supplemented with organic acids and nucleotides on growth, immune responses and digestive tract development in weaned pigs.. J Anim Physiol Anim Nutr 2007;91:508–18.
    pubmed: 17988355
  76. Sauer N, Eklund M, Bauer E, Gänzle MG, Field CJ, Zijlstra RT. The effects of pure nucleotides on performance, humoral immunity, gut structure and numbers of intestinal bacteria of newly weaned pigs.. J Anim Sci 2012;90:3126–34.
    pubmed: 22859755
  77. Superchi P, Saleri R, Borghetti P, Angelis E, Ferrari L, Cavalli V. Effects of dietary nucleotide supplementation on growth performance and hormonal and immune responses of piglets.. Animal : an international journal of animal bioscience 2012;6:902–8.
    pubmed: 22558960
  78. Cameron BF, Wong CW, Hinch GN, Singh D, Nolan JV, Colditz IG. Effects of nucleotides on the immune function of early-weaned piglets.. In: Digestive physiology of pigs proceedings of the 8th Symposium, Swedish University of Agricultural Sciences, Uppsala, Sweden, 20–22 June 2000. 2001. p. 66–8.
  79. Jang KB, Kim SW. Supplemental effects of dietary nucleotides on intestinal health and growth performance of newly weaned pigs.. J Anim Sci 2019;97:4875–82.
    pmc: PMC6915224pubmed: 31665463
  80. Cosgrove M, Davies DP, Jenkins HR. Nucleotide supplementation and the growth of term small for gestational age infants.. Archives of Disease in Childhood - Fetal and Neonatal Edition 1996;74:F122–5.
    pmc: PMC2528517pubmed: 8777659
  81. Singhal A, Macfarlane G, Macfarlane S, Lanigan J, Kennedy K, Elias-Jones A. Dietary nucleotides and fecal microbiota in formula-fed infants: a randomized controlled trial.. Am J Clin Nutr 2008;87:1785–92.
    pubmed: 18541569
  82. Liu Y, Xie C, Zhai Z, Deng Z, Jonge HRD, Wu X. Uridine attenuates obesity, ameliorates hepatic lipid accumulation and modifies the gut microbiota composition in mice fed with a high-fat diet.. Food Funct 2021;12:1829–40.
    pubmed: 33527946
  83. Boutard M, Cerisy T, Nogue P-Y, Alberti A, Weissenbach J, Salanoubat M. Functional diversity of carbohydrate-active enzymes enabling a bacterium to ferment plant biomass.. PLoS Genet 2014;10: e1004773.
    pmc: PMC4230839pubmed: 25393313
  84. Wunderlich G, Bull M, Ross T, Rose M, Chapman B. Understanding the microbial fibre degrading communities, processes in the equine gut.. Anim microbiome 2023;5:3.
    pmc: PMC9837927pubmed: 36635784
  85. Li N, Yan F, Wang N, Song Y, Yue Y, Guan J. Distinct gut microbiota and metabolite profiles induced by different feeding methods in healthy Chinese infants.. Front Microbiol 2020;11:11.
    pmc: PMC7219020pubmed: 32435235
  86. Geor RJ, Harris PA. How to minimize gastrointestinal disease associated with carbohydrate nutrition in horses.. In: Proceedings of the annual convention of the AAEP. 2007. p. 178–85.
  87. Vermorel M, Martin-Rosset W. Concepts, scientific bases, structure and validation of the French horse net energy system (UFC).. Livest Prod Sci 1997;47:261–75.
  88. Berg EL, Fu CJ, Porter JH, Kerley MS. Fructooligosaccharide supplementation in the yearling horse: effects on fecal pH, microbial content, and volatile fatty acid concentrations.. J Anim Sci 2005;83:1549–53.
    pubmed: 15956463
  89. Julliand V, de Fombelle A, Drogoul C, Jacotot E. Feeding and microbial disorders in horses: part 3—effects of three hay:grain ratios on microbial profile and activities.. J Equine Vet 2001;21:543–6.
  90. Bergman EN. Energy contributions of volatile fatty acids from the gastrointestinal tract in various species.. Physiol Rev 1990;70:567–90.
    pubmed: 2181501
  91. Whitfield-Cargile CM, Chung HC, Coleman MC, Cohen ND, Chamoun-Emanuelli AM, Ivanov I. Integrated analysis of gut metabolome, microbiome, and exfoliome data in an equine model of intestinal injury.. Microbiome 2024;12:74.
    pmc: PMC11017594pubmed: 38622632
  92. Gorosito AR, Russell JB, Van Soest PJ. Effect of carbon-4 and carbon-5 volatile fatty acids on digestion of plant cell wall in vitro1.. J Dairy Sci 1985;68:840–7.

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