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Home / Equine Research Bank / Purinergic Signal / Distribution of purinergic P2X receptors in the equine digit...
Purinergic signalling2013; 9(3); 383-393; doi: 10.1007/s11302-013-9356-5

Distribution of purinergic P2X receptors in the equine digit, cervical spinal cord and dorsal root ganglia.

Abstract: Purinergic pathways are considered important in pain transmission, and P2X receptors are a key part of this system which has received little attention in the horse. The aim of this study was to identify and characterise the distribution of P2X receptor subtypes in the equine digit and associated vasculature and nervous tissue, including peripheral nerves, dorsal root ganglia and cervical spinal cord, using PCR, Western blot analysis and immunohistochemistry. mRNA signal for most of the tested P2X receptor subunits (P2X1-5, 7) was detected in all sampled equine tissues, whereas P2X6 receptor subunit was predominantly expressed in the dorsal root ganglia and spinal cord. Western blot analysis validated the specificity of P2X1-3, 7 antibodies, and these were used in immunohistochemistry studies. P2X1-3, 7 receptor subunits were found in smooth muscle cells in the palmar digital artery and vein with the exception of the P2X3 subunit that was present only in the vein. However, endothelial cells in the palmar digital artery and vein were positive only for P2X2 and P2X3 receptor subunits. Neurons and nerve fibres in the peripheral and central nervous system were positive for P2X1-3 receptor subunits, whereas glial cells were positive for P2X7 and P2X1 and 2 receptor subunits. This previously unreported distribution of P2X subtypes may suggest important tissue specific roles in physiological and pathological processes.
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Publication Date: 2013-02-06 PubMed ID: 23381684PubMed Central: PMC3757141DOI: 10.1007/s11302-013-9356-5Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research study explores the distribution of P2X receptors, which are significant in pain transmission, in specific equine tissues. It uses PCR, Western blot analysis, and immunohistochemistry to identify P2X receptor subtypes in the equine digit and associated structures.

Research Methodology

  • The researchers utilized Polymerase Chain Reaction (PCR), Western blot analysis, and immunohistochemistry to determine the presence and distribution of P2X receptor subtypes across different equine tissues.
  • They specifically looked at tissues in the equine digit (an anatomical part that refers to the end of the limb, like a finger or a toe in humans), its associated vasculature, and nerves; as well as in the dorsal root ganglia (clusters of nerve cells bodies) and cervical spinal cord.

Findings

  • The mRNA signal for most of the tested P2X receptor subunits (P2X1-5, 7) was detected in all the equine tissues sampled. However, the P2X6 receptor subunit was predominantly found in the dorsal root ganglia and spinal cord.
  • Through Western blot analysis, the study validated the specificity of P2X1-3, 7 antibodies. These were subsequently used in immunohistochemistry studies.
  • The researchers found P2X1-3, 7 receptor subunits in smooth muscle cells in the palmar digital artery and vein with the sole exception of the P2X3 subunit, which was only found in the vein. The endothelial cells in the same locations were only positive for P2X2 and P2X3 receptor subunits.
  • In the peripheral and central nervous system, neurons and nerve fibres were positive for P2X1-3 receptor subunits, while glial cells (non-neuronal cells that provide support and protection for neurons) were positive for the P2X7 and P2X1 and 2 receptor subunits.

Implications

  • The distribution of P2X receptor subtypes across the studied equine tissues suggests potential tissue-specific roles in physiological and pathological processes. This marks a previously unexplored area in the study of purinergic pathways, particularly in relation to equine species.
  • The findings could shed light on understanding pain transmission in horses and eventually contribute to developing better pain management strategies for both equine and potentially human medicine.

Cite This Article

APA
Zamboulis DE, Senior JM, Clegg PD, Gallagher JA, Carter SD, Milner PI. (2013). Distribution of purinergic P2X receptors in the equine digit, cervical spinal cord and dorsal root ganglia. Purinergic Signal, 9(3), 383-393. https://doi.org/10.1007/s11302-013-9356-5

Publication

Purinergic signalling
ISSN: 1573-9546
NlmUniqueID: 101250499
Country: Netherlands
Language: English
Volume: 9
Issue: 3
Pages: 383-393

Researcher Affiliations

Zamboulis, D E
  • Faculty of Health and Life Sciences, Department of Musculoskeletal Biology, Institute of Ageing and Chronic Disease, University of Liverpool, Leahurst Campus, Chester High Road, Neston, Cheshire, CH64 7TE, UK.
Senior, J M
    Clegg, P D
      Gallagher, J A
        Carter, S D
          Milner, P I

            MeSH Terms

            • Animals
            • Arteries / metabolism
            • Blotting, Western
            • Cervical Vertebrae
            • Ganglia, Spinal / metabolism
            • Hoof and Claw / blood supply
            • Hoof and Claw / innervation
            • Hoof and Claw / metabolism
            • Horses
            • Immunohistochemistry
            • RNA, Messenger / analysis
            • Receptors, Purinergic P2X / analysis
            • Receptors, Purinergic P2X / biosynthesis
            • Reverse Transcriptase Polymerase Chain Reaction
            • Spinal Cord / metabolism
            • Veins / metabolism

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            This article has been cited 6 times.
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