Effect of a dietary antioxidant supplementation on semen quality in pony stallions.
Abstract: Lipid peroxidation contributes to the damage of the sperm plasma membrane. In different species, dietary supplementation with antioxidants has been shown to improve semen quality. Therefore, we tested effects of dietary supplementation with antioxidants and l-carnitin on semen quality in Shetland pony stallions (n=6). Semen was collected twice a week over a time period of 16 weeks. From weeks 5 to 12, a special diet for stallions containing a variety of antioxidants (STALLION, Pavo Pferdenahrung GmbH, Goch, Germany; tocopherol 300 mg/day; ascorbic acid 300 mg/day; l-carnitin 4000 mg/day; folic acid 12 mg/day) was added to the basal diet (hay, mineral supplements, water). Ejaculates were evaluated for total sperm count, semen motility (percentage of totally and progressively motile spermatozoa, longevity for 24 h at 5 degrees C) and membrane integrity (SYBR-14/PI staining): All values given are means+/-S.E.M. No changes in motility, progressive motility and membrane integrity or semen longevity for 24 h were detected. A slight but significant reduction of morphologically abnormal spermatozoa was found (weeks 1-4: 43.7+/-7.1%; weeks 13-16: 39.4+/-7.2%, p<0.05). Results show that a supplementary diet with antioxidants in the given concentration and duration does not result in pronounced effects on semen quality of stallions. It is therefore questionable to support stallions with dietary antioxidants as long as they receive an adequately balanced basal diet.
Publication Date: 2008-03-20 PubMed ID: 18358523DOI: 10.1016/j.theriogenology.2008.01.007Google Scholar: Lookup
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- Journal Article
Summary
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The study investigates the impact of dietary antioxidant supplementation on semen quality in pony stallions, finding that no significant changes in semen quality attributes, except for a slight decrease in morphologically abnormal spermatozoa.
Objective and Methodology of the Study
- The main objective of this research was to investigate the effect of dietary supplementation with antioxidants and l-carnitin on semen quality in Shetland pony stallions.
- They tested a special diet for six Shetland pony stallions. This diet was added to stallions’ daily feed of hay, mineral supplements, and water. It contained antioxidants including tocopherol, ascorbic acid, and folic acid, along with l-carnitin for a total of 8 weeks.
- Semen was collected twice a week over a time period of 16 weeks. Semen samples were examined for total sperm count, semen motility, semen longevity, and membrane integrity.
Results of the Study
- The research found that supplementing pony stallions’ diet with antioxidants did not lead to significant improvements in semen quality in terms of motility, progressive motility, membrane integrity, or longevity of semen.
- The only notable change the researchers found was a slight, yet statistically significant reduction in the proportion of morphologically abnormal spermatozoa after the diet intervention.
Conclusion and Implications
- The result indicates that the impact of antioxidant supplementation on semen quality in pony stallions might be minimal and might not lead to statistically meaningful improvements in general semen quality parameters.
- It also raises the question of whether it is necessary to supplement stallions’ diets with antioxidants when they are already consuming a well-balanced diet. This seems to suggest that as long as a balanced diet ascertained, additional dietary supplementation may not be required.
- However, the observed reduction of abnormal spermatozoa does imply that dietary supplementation with antioxidants may have some minor beneficial effects on sperm morphology, an aspect that might worth further investigations.
Cite This Article
APA
Deichsel K, Palm F, Koblischke P, Budik S, Aurich C.
(2008).
Effect of a dietary antioxidant supplementation on semen quality in pony stallions.
Theriogenology, 69(8), 940-945.
https://doi.org/10.1016/j.theriogenology.2008.01.007 Publication
Researcher Affiliations
- Centre for Artificial Insemination and Embryo Transfer, Department for Animal Breeding and Reproduction, University for Veterinary Sciences, Veterinärplatz 1, A-1210 Vienna, Austria.
MeSH Terms
- Animals
- Antioxidants / pharmacology
- Carnitine / pharmacology
- Cell Membrane / drug effects
- Cell Membrane / physiology
- Dietary Supplements
- Horses / physiology
- Male
- Microscopy, Fluorescence / veterinary
- Semen / drug effects
- Semen / physiology
- Sperm Count / veterinary
- Sperm Motility / drug effects
- Sperm Motility / physiology
- Spermatozoa / abnormalities
- Spermatozoa / drug effects
- Statistics, Nonparametric
Citations
This article has been cited 5 times.- Bazzano M, Laus F, Spaterna A, Marchegiani A. Use of nutraceuticals in the stallion: Effects on semen quality and preservation. Reprod Domest Anim 2021 Jul;56(7):951-957.
- Cappai MG, Taras A, Cossu I, Cherchi R, Dimauro C, Accioni F, Boatto G, Deroma M, Spanu E, Gatta D, Dall'Aglio C, Pinna W. Effects of Dietary Zn/Se and α-Tocopherol Supplementation on Metabolic Milieu, Haemogram and Semen Traits of Breeding Stallions. Biol Trace Elem Res 2021 Sep;199(9):3287-3296.
- Ullah A, Chen W, Shi L, Wang M, Geng M, Na J, Akhtar MF, Khan MZ, Wang C. Challenges and Enhancing Strategies of Equine Semen Preservation: Nutritional and Genetic Perspectives. Vet Sci 2025 Aug 25;12(9).
- Díaz Rojas E, Carrillo Moreno DI, Contreras Villarreal V, Arellano Rodríguez F, Alvarado Espino AS, Ángel García O. Effect of nutraceutical supplementation on semen quality in stallions. Vet Med Sci 2023 Nov;9(6):2600-2605.
- Catandi GD, LiPuma L, Obeidat YM, Maclellan LJ, Broeckling CD, Chen T, Chicco AJ, Carnevale EM. Oocyte metabolic function, lipid composition, and developmental potential are altered by diet in older mares. Reproduction 2022 Apr 1;163(4):183-198.
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