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Home / Equine Research Bank / Am J Stem Cells / Effect of extracorporeal shock wave on proliferation and dif...
American journal of stem cells2013; 2(1); 62-73;

Effect of extracorporeal shock wave on proliferation and differentiation of equine adipose tissue-derived mesenchymal stem cells in vitro.

Abstract: Mesenchymal stem cells are regarded as common cellular precursors of the musculoskeletal tissue and are responsible for tissue regeneration in the course of musculoskeletal disorders. In equine veterinary medicine extracorporeal shock wave therapy (ESWT) is used to optimize healing processes of bone, tendon and cartilage. Nevertheless, little is known about the effects of the shock waves on cells and tissues. Thus, the aim of this study was to investigate the influence of focused ESWT on the viability, proliferation, and differentiation capacity of adipose tissue-derived mesenchymal stem cells (ASCs) and to explore its effects on gap junctional communication and the activation of signalling cascades associated with cell proliferation and differentiation. ASCs were treated with different pulses of focused ESWT. Treated cells showed increased proliferation and expression of Cx43, as detected by means of qRT-PCR, histological staining, immunocytochemistry and western blot. At the same time, cells responded to ESWT by significant activation (phosphorylation) of Erk1/2, detected in western blots. No significant effects on the differentiation potential of the ASCs were evident. Taken together, the present results show significant effects of shock waves on stem cells in vitro.
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Publication Date: 2013-03-08 PubMed ID: 23671817PubMed Central: PMC3636727
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates how extracorporeal shock wave therapy (ESWT) can improve the proliferation and differentiation of adipose tissue-derived mesenchymal stem cells (ASCs) in horses. The study also looked into the effects ESWT has on cellular processes and signalling associated with cell proliferation and differentiation.

Research Methodology

  • The study aimed to understand the effect of ESWT on the viability, proliferation, and differentiation ability of ASCs.
  • Gap junctional communication and activation of signalling cascades related to cell proliferation and differentiation were also looked into.
  • ASCs were exposed to various pulses of focused ESWT. Various protocols such as qRT-PCR, histological staining, immunocytochemistry, and western blot were employed to determine the impact.

Research Findings

  • The research found that following ESWT, the ASCs exhibited increased proliferation and elevated expression of Cx43.
  • The research also documented the significant activation or phosphorylation of Erk1/2 in response to ESWT. This was detected via western blots.
  • However, there were no substantial effects recorded on the differentiation capacity of the ASCs following ESWT treatment.

Conclusion

  • Although ESWT did not significantly affect ASC differentiation, it demonstrated prominent impacts on ASC proliferation.
  • This finding could have considerable implications for veterinary medicine, particularly in improving healing processes in equines’ bones, tendons, and cartilage.
  • The insight from this study, which focuses on ESWT’s effects on stem cells, could potentially inform future research or treatments centered on tissue regeneration.

Cite This Article

APA
Raabe O, Shell K, Goessl A, Crispens C, Delhasse Y, Eva A, Scheiner-Bobis G, Wenisch S, Arnhold S. (2013). Effect of extracorporeal shock wave on proliferation and differentiation of equine adipose tissue-derived mesenchymal stem cells in vitro. Am J Stem Cells, 2(1), 62-73.

Publication

American journal of stem cells
ISSN: 2160-4150
NlmUniqueID: 101578936
Country: United States
Language: English
Volume: 2
Issue: 1
Pages: 62-73

Researcher Affiliations

Raabe, O
  • Institute of Veterinary -Anatomy, -Histology, and -Embryology, Justus-Liebig University of Giessen Germany.
Shell, K
    Goessl, A
      Crispens, C
        Delhasse, Y
          Eva, A
            Scheiner-Bobis, G
              Wenisch, S
                Arnhold, S

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