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Home / Equine Research Bank / Can J Vet Res / Effect of extracorporeal shockwave therapy on the immunomodu...
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire2024; 88(3); 87-93; doi: 10.1111/j.2042-3306.2009.00021.x

Effect of extracorporeal shockwave therapy on the immunomodulatory and anti-inflammatory properties of cultured equine umbilical cord blood mesenchymal stromal cells.

Abstract: There is a knowledge gap regarding the effect of extracorporeal shockwave treatment (ESWT) on the stress response and immunomodulatory and anti-inflammatory properties of equine umbilical cord blood mesenchymal stromal cells (CB-MSCs). The objective of this study was to investigate the presence of cellular oxidative stress, inflammatory response, and production of growth factors in CB-MSCs after treatment with ESWT. We hypothesized that CB-MSCs treated with ESWT will experience higher levels of cellular stress and increased production of anti-inflammatory cytokines and growth factors compared to untreated CB-MSCs. Il existe un manque de connaissances concernant l’effet du traitement extracorporel par ondes de choc (ESWT) sur la réponse au stress et les propriétés immunomodulatrices et anti-inflammatoires des cellules stromales mésenchymateuses du sang de cordon ombilical équin (CB-MSCs). L’objectif de cette étude était d’étudier la présence de stress oxydatif cellulaire, de réponse inflammatoire et de production de facteurs de croissance dans les CB-MSCs après un traitement par ESWT. Nous avons émis l’hypothèse que les CB-MSCs traitées par ESWT connaîtront des niveaux plus élevés de stress cellulaire et une production accrue de cytokines anti-inflammatoires et de facteurs de croissance par rapport aux CB-MSCs non traitées.(Traduit par Docteur Serge Messier).
Copyright and/or publishing rights held by the Canadian Veterinary Medical Association.
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Publication Date: 2024-07-11 PubMed ID: 38988333PubMed Central: PMC11235386DOI: 10.1111/j.2042-3306.2009.00021.xGoogle Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigated whether treating equine umbilical cord blood mesenchymal stromal cells with extracorporeal shockwave therapy leads to increased cellular stress and production of anti-inflammatory cytokines and growth factors.

Objective and Hypothesis of the Study

  • The research aimed to understand the impact of extracorporeal shockwave therapy (ESWT) on equine umbilical cord blood mesenchymal stromal cells (CB-MSCs).
  • This treatment was explored in the context of its effects on the stress response and whether it alters the immunomodulatory and anti-inflammatory properties of these cells.
  • The study hypothesized that treating these cells with ESWT increases cellular stress and leads to upregulated production of anti-inflammatory cytokines – proteins that modulate the body’s immune response – and growth factors, which stimulate cell growth.

Relevance of the Research

  • This research is essential to understand the implications of ESWT treatment on cellular stress responses and immunomodulatory functions of CB-MSCs. It could give a new perspective for CB-MSC applications in cell-based therapies.
  • CB-MSCs present a robust therapeutic potential for a variety of disorders due to their anti-inflammatory and immunomodulatory properties. Gaining in-depth knowledge about how these properties and cellular stress levels are influenced by ESWT is important for therapeutic developments.
  • The research contributes to filling a knowledge gap regarding the effects of ESWT on CB-MSCs, given that this kind of treatment is widely used in veterinary and human medicine to enhance healing and tissue regeneration.

Treatment and Investigation

  • The research investigated the impact of ESWT treatment on the presence of cellular oxidative stress, inflammatory response, and production of growth factors in CB-MSCs.
  • Oxidative stress is a state of imbalance due to excessive reactive oxygen species that can damage cells. ESWT treatment could potentially elevate the levels of oxidative stress on the cells.
  • The inflammatory response and production of growth factor in cells post-treatment can provide insights into how ESWT can modulate the immune response and affect cellular growth.

Cite This Article

APA
Giraldo A, Koch TG, Madan P, Lepage S, Monteith G, Alizadeh AH, Tran A, Mortagy N, Koenig JB. (2024). Effect of extracorporeal shockwave therapy on the immunomodulatory and anti-inflammatory properties of cultured equine umbilical cord blood mesenchymal stromal cells. Can J Vet Res, 88(3), 87-93. https://doi.org/10.1111/j.2042-3306.2009.00021.x

Publication

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire
ISSN: 1928-9022
NlmUniqueID: 8607793
Country: Canada
Language: English
Volume: 88
Issue: 3
Pages: 87-93

Researcher Affiliations

Giraldo, Andrés
  • Departments of Clinical Studies (Giraldo, Monteith, Koenig) and Biomedical Sciences (Koch, Madan, Lepage, Alizadeh, Mortagy, Tran), Ontario Veterinary College, University of Guelph, Guelph, Ontario.
Koch, Thomas G
  • Departments of Clinical Studies (Giraldo, Monteith, Koenig) and Biomedical Sciences (Koch, Madan, Lepage, Alizadeh, Mortagy, Tran), Ontario Veterinary College, University of Guelph, Guelph, Ontario.
Madan, Pavneesh
  • Departments of Clinical Studies (Giraldo, Monteith, Koenig) and Biomedical Sciences (Koch, Madan, Lepage, Alizadeh, Mortagy, Tran), Ontario Veterinary College, University of Guelph, Guelph, Ontario.
Lepage, Sarah
  • Departments of Clinical Studies (Giraldo, Monteith, Koenig) and Biomedical Sciences (Koch, Madan, Lepage, Alizadeh, Mortagy, Tran), Ontario Veterinary College, University of Guelph, Guelph, Ontario.
Monteith, Gabrielle
  • Departments of Clinical Studies (Giraldo, Monteith, Koenig) and Biomedical Sciences (Koch, Madan, Lepage, Alizadeh, Mortagy, Tran), Ontario Veterinary College, University of Guelph, Guelph, Ontario.
Alizadeh, Amir H
  • Departments of Clinical Studies (Giraldo, Monteith, Koenig) and Biomedical Sciences (Koch, Madan, Lepage, Alizadeh, Mortagy, Tran), Ontario Veterinary College, University of Guelph, Guelph, Ontario.
Tran, Andy
  • Departments of Clinical Studies (Giraldo, Monteith, Koenig) and Biomedical Sciences (Koch, Madan, Lepage, Alizadeh, Mortagy, Tran), Ontario Veterinary College, University of Guelph, Guelph, Ontario.
Mortagy, Narman
  • Departments of Clinical Studies (Giraldo, Monteith, Koenig) and Biomedical Sciences (Koch, Madan, Lepage, Alizadeh, Mortagy, Tran), Ontario Veterinary College, University of Guelph, Guelph, Ontario.
Koenig, Judith B
  • Departments of Clinical Studies (Giraldo, Monteith, Koenig) and Biomedical Sciences (Koch, Madan, Lepage, Alizadeh, Mortagy, Tran), Ontario Veterinary College, University of Guelph, Guelph, Ontario.

MeSH Terms

  • Animals
  • Horses
  • Mesenchymal Stem Cells
  • Fetal Blood / cytology
  • Extracorporeal Shockwave Therapy / methods
  • Cytokines / metabolism
  • Cells, Cultured

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