English
French
Effect of platelet lysate on Schwann-like cell differentiation of equine bone marrow-derived mesenchymal stem cells.
Abstract: Currently, treatment for peripheral nerve injuries in horses primarily relies upon physical therapy and anti-inflammatory drugs. In humans, various treatments using mesenchymal stem cells (MSCs) are being attempted. Therefore, in this study, Schwann-like cell differentiation cultures of equine MSCs were prepared using fetal bovine serum (FBS) and equine platelet lysate (ePL). ePL increased the platelet count to 1 × 10/μl, the optimal concentration for culture. In both groups, an elongated morphology at both ends, characteristic of Schwann cells, was observed under the microscope. Real-time PCR analysis of the expression levels of neuronal markers showed that the ePL group tended to express higher levels of Nestin, Musashi1, and Pax3 than the FBS group. p75 was expressed at low levels in both groups. Immunostaining results showed localization of Nestin in both groups of differentiated cells, but the positive cell rate was significantly higher in the ePL group than in the FBS group. Overall, the ePL gro showed good results for Schwann-like cell differentiation, which may be useful for future use in the treatment of equine motor neuron disease. This knowledge could be applied translationaly in the treatment of amyotrophic lateral sclerosis in humans.Overall, the ePL group showed good results for Schwann-like cell differentiation, which may be useful for future use in the treatment of equine motor neuron disease and in the treatment of amyotrophic lateral sclerosis in humans.
Copyright © 2023 Elsevier Ltd. All rights reserved.
Publication Date: 2023-03-31 PubMed ID: 37060838DOI: 10.1016/j.rvsc.2023.03.023Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Amyotrophic Lateral Sclerosis
- Animal Science
- Biotechnology
- Bone Marrow
- Cell Culture
- Cell Proliferation
- Disease Treatment
- Equine Diseases
- Equine Health
- Equine Motor Neuron Disease
- Equine Science
- Genetics
- In Vitro Research
- Mesenchymal Cells
- Neurological Diseases
- Platelets
- Stem Cells
- Veterinary Medicine
- Veterinary Research
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This study investigates the potential of using equine platelet lysate to support the differentiation of equine mesenchymal stem cells into Schwann-like cells, which are necessary for nerve repair. The researchers found that the platelet lysate is conducive to creating these cells and could potentially be applied for the treatment of equine motor neuron disease and even in human conditions like amyotrophic lateral sclerosis.
Methodology and Observation
- The study was designed to evaluate a treatment for peripheral nerve injuries in horses based on a method already in use for humans, involving the transformation of mesenchymal stem cells (MSCs) into Schwann-like cells.
- Two different cultures of these Schwann-like cells were prepared: one using fetal bovine serum (FBS) and the other using equine platelet lysate (ePL). The use of ePL led to an increase in platelet count, which was considered optimal for the culture.
- Observation under a microscope showed that cells in both groups developed an elongated appearance at both ends, a characteristic of Schwann cells.
Comparative Analysis and Findings
- A comparative analysis was conducted between the two groups using real-time polymerase chain reaction (PCR) to measure gene expression levels. It was observed that the platelet lysate group had a higher expression of Nestin, Musashi1, and Pax3- genes that are associated with nerve cells.
- The p75 gene, which plays a role in cell death and survival, was expressed at low levels in both groups.
- Immunostaining revealed Nestin localization in both cultures, but significantly more so in the ePL group.
Implication of Study and Future Application
- The study indicates that use of ePL for Schwann-like cell differentiation yields better results than FBS.
- The findings from this study could be beneficial in treating equine peripheral nerve injuries.
- Furthermore, this study has potential translational implications for human diseases such as amyotrophic lateral sclerosis, which is characterized by degeneration of motor neurons in the brain and spinal cord.
Cite This Article
APA
Fujiwara Y, Kusakabe KT, Baba K, Sasaki N.
(2023).
Effect of platelet lysate on Schwann-like cell differentiation of equine bone marrow-derived mesenchymal stem cells.
Res Vet Sci, 159, 11-18.
https://doi.org/10.1016/j.rvsc.2023.03.023 Publication
Researcher Affiliations
- Laboratory of Large Animal Surgery, Department of Clinical Veterinary Science, Yamaguchi University of Joint Faculty of Veterinary Medicine, Yamaguchi-city, Yamaguchi 753-8515, Japan.
- Laboratory of Veterinary Anatomy, Department of Clinical Veterinary Science, Yamaguchi University of Joint Faculty of Veterinary Medicine, Yamaguchi-city, Yamaguchi 753-8515, Japan.
- Laboratory of Veterinary Internal Medicine, Department of Clinical Veterinary Science, Yamaguchi University of Joint Faculty of Veterinary Medicine, Yamaguchi-city, Yamaguchi 753-8515, Japan.
- Laboratory of Large Animal Surgery, Department of Clinical Veterinary Science, Yamaguchi University of Joint Faculty of Veterinary Medicine, Yamaguchi-city, Yamaguchi 753-8515, Japan. Electronic address: nsasaki@yamaguchi-u.ac.jp.
MeSH Terms
- Humans
- Animals
- Horses
- Nestin / metabolism
- Nestin / pharmacology
- Amyotrophic Lateral Sclerosis / metabolism
- Amyotrophic Lateral Sclerosis / veterinary
- Bone Marrow
- Cell Differentiation / physiology
- Mesenchymal Stem Cells
- Cells, Cultured
- Horse Diseases / therapy
- Horse Diseases / metabolism
Conflict of Interest Statement
Declaration of Competing Interest The authors declare no financial or personal conflicts of interest.
Citations
This article has been cited 0 times.Secure Payments
Satisfaction Guarantee
We offer a 60-day guarantee to ensure you are completely satisfied.
Fast & Free Shipping
We provide fast and free shipping on all orders over $40.