Effect of repeated collection of multiple endometrial biopsy specimens on subsequent pregnancy in mares.

The research investigated the impact of multiple endometrial biopsy collections on the pregnancy rate of horses. The study found that the frequency of these biopsies did not negatively affect the likelihood of pregnancy.

Study Objective and Methodology

• The main purpose of the study was to assess the effect of repeated endometrial biopsy collections on the pregnancy outcome in mares.
• In the experiment, 4 or 5 endometrial biopsy specimens were gathered from each of the seven mares involved. This collection process was repeated 2, 3, or 4 times over a 50-day period before breeding.
• Additionally, four of these specimen collection days were undertaken within 6 days prior to breeding.

Results and Observations

• On the 14th day after ovulation, six out of the seven mares were confirmed pregnant through ultrasonography.
• Interestingly, the research found that the pregnancy rate of the mares matched the rate achieved when the same mares were bred at estrus before the commencement of the study.
• This suggests that the repeated collection of endometrial biopsy samples did not reduce the chances of the mares getting pregnant.

Conclusion and Clinical Relevance

• The research concludes that frequent collection of multiple endometrial biopsy specimens from mares who are genitally normal does not have a negative impact on their pregnancy rates.
• This conclusion is clinically relevant as it indicates that endometrial biopsies, which are crucial in diagnosing various reproductive disorders in mares, can be conducted regularly without negatively affecting their fertility.