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Animals : an open access journal from MDPI2022; 12(23); doi: 10.3390/ani12233340

Effects of Differences in Fibre Composition and Maturity of Forage-Based Diets on the Fluid Balance, Water-Holding Capacity and Viscosity in Equine Caecum and Colon Digesta.

Abstract: Horses are herbivores, and their hindgut functions as a fluid reservoir as forage fibre properties have great impact on the water content of digesta and the milieu in the ecosystem. Our objective was to compare the effect of grass fibre maturity and legume forage on the water-holding capacity (WHC) and viscosity of the equine hindgut and the body weight (BW) and fluid balance of horses. Three diets: concentrate and late harvested grass haylage (35:65 energy ratio) (C); early and late harvested grass haylage (80:20) (G); lucerne and late harvested grass haylage (80:20) (L) were fed to six caecum and colon fistulated horses for 28 days in a Latin-square design. Total water intake and BW were higher when the horses were fed Diet L, but the digesta WHC was higher when fed Diet G. Total water excretion (via faeces + urine) and the difference in total water intake-output was higher when fed Diet L. Viscosity, measured on centrifuged digesta fluid, did not differ between diets, but the individual colon data of one horse were higher. In conclusion, early harvested forage might be beneficial for the fluid balance of athletic horses providing a higher WHC of hindgut digesta without increasing BW. The importance of digesta viscosity in relation to equine diets needs further investigations.
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Publication Date: 2022-11-29 PubMed ID: 36496860PubMed Central: PMC9739581DOI: 10.3390/ani12233340Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study explores the impact of differences in fibre composition and maturity of forage-based diets on the fluid balance and water-holding capacity in equine stomach and colon digesta. Findings suggest that early harvested forage might benefit athletic horses’ fluid balance by increasing hindgut digesta’s water-holding capacity without effecting body weight.

Research Objectives and Procedure

  • The research aimed to examine how the properties of forage fibre, particularly differences in fibre composition and maturity, influence the fluid balance, water-holding capacity (WHC) and viscosity in horse’s caecum and colon.
  • Six horses, which had undergone fistula surgery in their caecum and colon, were selected. They were fed three different diets over a period of 28 days in a Latin-square design to control the influence of external factors.
  • The three diets included a mixture of concentrate and late harvested grass haylage (C); a combination of early and late harvested grass haylage (G); and a blend of lucerne and late harvested grass haylage (L).

Results and Findings

  • It was observed that the horses had higher total water intake and body weight when they were fed Diet L. However, the water-holding capacity of digesta was greater when they were given Diet G.
  • Total water excretion, which is the sum of faeces and urine, and the difference between total water intake and output was higher in the horses when they were on Diet L.
  • There were no significant differences in the viscosity of the digesta fluid among the horses on different diets, although individual data from one horse’s colon showed a higher viscosity.

Conclusions and Future Research

  • From this study, the researchers concluded that early harvested forage could be beneficial for the fluid balance in athletic horses by increasing the water-holding capacity of hindgut digesta without significantly affecting body weight.
  • However, the researchers noted that more research is required to further investigate the importance of digesta viscosity in relation to equine diets.

Cite This Article

APA
Muhonen S, Philippeau C, Julliand V. (2022). Effects of Differences in Fibre Composition and Maturity of Forage-Based Diets on the Fluid Balance, Water-Holding Capacity and Viscosity in Equine Caecum and Colon Digesta. Animals (Basel), 12(23). https://doi.org/10.3390/ani12233340

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 12
Issue: 23

Researcher Affiliations

Muhonen, Sara
  • L'Institut Agro Dijon, Burgundy-Franche-Comté University, PAM UMR A 02.102, 21000 Dijon, France.
Philippeau, Christelle
  • L'Institut Agro Dijon, 26 Boulevard Dr. Petitjean, BP87999, 21079 Dijon, France.
Julliand, Véronique
  • L'Institut Agro Dijon, Burgundy-Franche-Comté University, PAM UMR A 02.102, 21000 Dijon, France.

Grant Funding

  • 2008-742 / Swedish Research Council for Environment Agricultural Sciences and Spatial Planning

Conflict of Interest Statement

The authors declare no conflict of interest.

References

This article includes 56 references
  1. Meyer H. Nutrition of the equine athlete.. In: Gillespie J.R., Robinson N.E., editors. Equine Exercise Physiology 2. ICEEP Publications; Davis, CA, USA: 1987. pp. 644–673.
  2. Carlson G.P. Haematology and body fluids in the equine athlete: A review.. In: Gillespie J.R., Robinson N.E., editors. Equine Exercise Physiology 2. ICEEP Publications; Davis, CA, USA: 1987. pp. 393–425.
  3. Spooner H.S, Nielsen B.D, Schott H.C. II, O’Connor-Robison C.I, Harris P.A. Hydration status of horses performing endurance exercise: I. Evidence for a role of diet.. Comp. Exerc. Physiol. 2013;9:189–197.
    doi: 10.3920/CEP13017google scholar: lookup
  4. Eastwood M.A. Vegetable fibre: Its physical properties. Proceedings of the Nutrition Society 32; Edinburgh, UK. 14 April 1973; pp. 137–143.
    doi: 10.1079/PNS19730031pubmed: 4605435google scholar: lookup
  5. Robertson J.A, Eastwood M.A, Yeoman M.M. An investigation into the physical properties of fibre prepared from several carrot varieties at different stages of development.. J. Sci. Food Agric. 1980;31:633–638.
    doi: 10.1002/jsfa.2740310703google scholar: lookup
  6. McBurney M.I, Horvath P.J, Jeraci J.L, Van Soest P.J. Effect of in vitro fermentation using human faecal inoculum on the water-holding capacity of dietary fibre.. Br. J. Nutr. 1985;53:17–24.
    doi: 10.1079/BJN19850005pubmed: 2998439google scholar: lookup
  7. Warren L.K, Lawrence L.M, Brewster-Barnes T, Powell D.M. The effect of dietary fibre on hydration status after dehydration with frusemide.. Equine Vet. J. 1999;31((Suppl. S30)):508–513.
    doi: 10.1111/j.2042-3306.1999.tb05275.xpubmed: 10659309google scholar: lookup
  8. Darlington J.M, Hershberger T.V. Effect of forage maturity on digestibility, intake and nutritive value of alfalfa, timothy and orchardgrass by equine.. J. Anim. Sci. 1968;27:1572–1576.
    doi: 10.2527/jas1968.2761572xgoogle scholar: lookup
  9. Ragnarsson S, Lindberg J.E. Nutritional value of timothy haylage in Icelandic horses.. Livest. Sci. 2008;113:202–208.
    doi: 10.1016/j.livsci.2007.03.010google scholar: lookup
  10. Hansen T.L, Lawrence L.M. Composition factors predicting forage digestibility by horses.. J. Equine Vet. Sci. 2017;58:97–102.
    doi: 10.1016/j.jevs.2017.08.015google scholar: lookup
  11. Stephen A.M, Cummings J.H. Mechanism of action of dietary fibre in the human colon.. Nature 1980;284:283–284.
    doi: 10.1038/284283a0pubmed: 7360261google scholar: lookup
  12. Hansen T.L, Chizek E.L, Zugay O.K, Miller J.M, Bobel J.M, Chouinard J.W, Adkin A.M, Skurupey L.A, Warren L.K. Digestibility and retention time of Coastal Bermudagrass (Cynodon dactylon) hay by horses.. Animals 2019;9:1148.
    doi: 10.3390/ani9121148pmc: PMC6940996pubmed: 31847350google scholar: lookup
  13. Buxon D.R, Redfearn D.D. Plant limitations to fiber digestion and utilization.. J. Nutr. 1997;127:814S–818S.
    doi: 10.1093/jn/127.5.814Spubmed: 9164243google scholar: lookup
  14. Hoffman P.C, Sievert S.J, Shaver R.D, Welch D.A, Combs D.K. In situ dry matter, protein, and fiber degradation of perennial forages.. J. Dairy Sci. 1993;76:2632–2643.
    doi: 10.3168/jds.S0022-0302(93)77599-2pubmed: 8227665google scholar: lookup
  15. Van Soest P.J, Robertson J.B, Lewis B.A. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition.. J. Dairy Sci. 1991;74:3583–3597.
    doi: 10.3168/jds.S0022-0302(91)78551-2pubmed: 1660498google scholar: lookup
  16. Argenzio R.A, Lowe J.E, Pickard D.W, Stevens C.E. Digesta passage and water exchange in the equine large intestine.. Am. J. Physiol. 1974;226:1035–1042.
    doi: 10.1152/ajplegacy.1974.226.5.1035pubmed: 4207578google scholar: lookup
  17. Argenzio R.A, Stevens C.E. Cyclic changes in ionic composition of digesta in the equine intestinal tract.. Am. J. Physiol. 1975;228:1224–1230.
    doi: 10.1152/ajplegacy.1975.228.4.1224pubmed: 1130521google scholar: lookup
  18. Schwabenbauer K, Meyer H, Lindemann G. Gehalt an flüchtigen Fettsäuren und Ammoniak im Caecuminhalt des Pferdes in Abhängigkeit von Futterart, Futterreihenfolge und Fütterungszeitpunkt.. Fortschr. Tierphysiol. Tierernährg. 1982;13:24–31.
    pubmed: 7084841
  19. Danielsen K, Lawrence L.M, Siciliano P, Powell D, Thompson K. Effect of diet on weight and plasma variables in endurance exercised horses.. Equine Vet. J. 1995;27((Suppl. S18)):372–377.
    doi: 10.1111/j.2042-3306.1995.tb04955.xgoogle scholar: lookup
  20. Meyer H. Influence of diet, exercise and water restriction on the gut fill in horses. Proceedings of the Equine Nutrition Physiology Society 14; Ontario, CA, USA. 19–21 January 1995; pp. 90–91.
  21. Rice O, Geor R, Harris P, Hoekstra K, Gardner S, Pagan J. Effects of restricted hay intake on body weight and metabolic responses to high-intensity exercise in Thoroughbred horses. Proceedings of the 17th Meeting of the Equine Nutritional and Physiology Society Lexington, KE, USA. 31 May–2 June 2001; pp. 273–279.
  22. Jansson A, Lindberg J.E. Effects of a forage-only diet on body weight and response to interval training on a track.. In: Saastamoinen M.T., Martin-Rosset W., editors. Nutrition of the Exercising Horse. Wageningen Academic Publishers; Wageningen, The Netherlands: 2008. pp. 345–349. EAAP Publication No. 125.
  23. Connysson M, Essén-Gustavsson B, Lindberg J.E, Jansson A. Effects of feed deprivation on standardbred horses fed a forage-only diet and a 50:50 forage-oats diet.. Equine Vet. J. 2010;42((Suppl. 38)):335–340.
    doi: 10.1111/j.2042-3306.2010.00174.xpubmed: 21059027google scholar: lookup
  24. Dikeman C.L, Fahey G.C. Jr. Viscosity as related to dietary fiber: A review.. Crit. Rev. Food Sci. Nutr. 2006;46:649–663.
    doi: 10.1080/10408390500511862pubmed: 17092830google scholar: lookup
  25. Lentle R.G, Janssen P.W.M. Physical characteristics of digesta and their influence on flow and mixing in the mammalian intestine: A review.. J. Comp. Physiol. B. 2008;178:673–690.
    doi: 10.1007/s00360-008-0264-xpubmed: 18401586google scholar: lookup
  26. Muhonen S, Sadet-Bourgeteau S, Julliand V. Effects of differences in fibre composition and maturity of forage-based diets on the microbial ecosystem and its activity in equine caecum and colon digesta and faeces.. Animals 2021;11:2337.
    doi: 10.3390/ani11082337pmc: PMC8388671pubmed: 34438794google scholar: lookup
  27. Lindgren E. The Nutritional Value of Roughages Determined In Vivo and by Laboratory Methods.. Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences; Uppsala, Sweden: 1979. p. 63. Report no. 45.
  28. Robertson J.A, Eastwood M.A. An examination of factors which may affect the water holding capacity of dietary fibre.. Br. J. Nutr. 1981;45:83–88.
    doi: 10.1079/BJN19810079pubmed: 6258627google scholar: lookup
  29. Muhonen S, Connysson M, Lindberg J.E, Julliand V, Bertilsson J, Jansson A. Effects of crude protein intake from grass silage-only diets on the equine colon ecosystem after an abrupt feed change.. J. Anim. Sci. 2008;86:3465–3472.
    doi: 10.2527/jas.2007-0374pubmed: 18676731google scholar: lookup
  30. Muhonen S, Julliand V, Lindberg J.E, Bertilsson J, Jansson A. Effects on the equine colon ecosystem of grass silage and haylage diets after an abrupt change from hay.. J. Anim. Sci. 2009;87:2291–2298.
    doi: 10.2527/jas.2008-1461pubmed: 19329474google scholar: lookup
  31. Jouany J.P, Medina B, Bertin G, Julliand V. Effect of live yeast culture supplementation on hindgut microbial communities and their polysaccharide and glycoside hydrolase activities in horses fed a high-fiber or high-starch diet.. J. Anim. Sci. 2009;87:2844–2852.
    doi: 10.2527/jas.2008-1602pubmed: 19465499google scholar: lookup
  32. Philippeau C, Sadet-Bourgeteau S, Varloud M, Julliand V. Impact of barley form on equine total tract fibre digestibility and colonic microbiota.. Animal 2015;9:1943–1948.
    doi: 10.1017/S1751731115001524pubmed: 26316024google scholar: lookup
  33. Ellis J.M, Hollands T, Allen D.E. Effect of forage intake on body weight and performance.. Equine Vet. J. 2002;34((Suppl. S34)):66–70.
    doi: 10.1111/j.2042-3306.2002.tb05393.xpubmed: 12405661google scholar: lookup
  34. Muhonen S, Julliand V. Effects of Differences in Fibre Composition and Maturity of Forage-Based Diets on the Fluid Balance, and Fecal Water-Holding Capacity and Microbial Ecosystem in French Trotters. AgroSup Dijon: Dijon, France. to be submitted.
    pmc: PMC9913277pubmed: 36766217
  35. Muhonen S. Metabolism and Hindgut Ecosystem in Forage Fed Sedentary and Athletic Horses.. Ph.D. Thesis. Acta Universitatis Agriculturae Sueciae; Uppsala, Sweden: 2008.
  36. Stephen A.M, Cummings J.H. Water-holding by dietary fibre in vitro and its relationship to faecal output in man.. Gut 1979;20:722–729.
    doi: 10.1136/gut.20.8.722pmc: PMC1412537pubmed: 488767google scholar: lookup
  37. Froetschel M.A, Amos H.E. Effects of dietary fiber and feeding frequency on ruminal fermentation, digesta water-holding capacity, and fractional turnover of contents.. J. Anim. Sci. 1991;69:1312–1321.
    doi: 10.2527/1991.6931312xpubmed: 1648068google scholar: lookup
  38. Owusu-Asiedu A, Patience J.F, Laarveld B, Van Kessel A.G, Simmins P.H, Zijlstra R.T. Effects of guar gum and cellulose on digesta passage rate, ileal microbial populations, energy and protein digestibility, and performance of grower pigs.. J. Anim. Sci. 2006;84:843–852.
    doi: 10.2527/2006.844843xpubmed: 16543561google scholar: lookup
  39. Bourquin L.D, Titgemeyer E.C, Fahey G.C. Jr. Fermentation of various dietary fiber sources by human fecal bacteria.. Nutr. Res. 1996;16:1119–1131.
    doi: 10.1016/0271-5317(96)00116-9google scholar: lookup
  40. Varloud M, de Fombelle A, Goachet A.G, Drogoul C, Julliand V. Partial and total apparent digestibility of dietary carbohydrates in horses as affected by the diet.. Anim. Sci. 2004;79:61–72.
    doi: 10.1017/S1357729800054539google scholar: lookup
  41. Hintz H.F, Hogue D.E, Walker E.F. Jr., Lowe J.E, Schryver H.F. Apparent digestion in various segments of the digestive tract of ponies fed diets with varying roughage-grain ratios.. J. Anim. Sci. 1971;32:245–248.
    doi: 10.2527/jas1971.322245xpubmed: 5543016google scholar: lookup
  42. Sperber I, Björnhag G, Holtenius K. A separation mechanism and fluid flow in the large intestine of the equine.. Pferdeheilkunde Sonderausg. 1992:29–32.
  43. Parsons A.M, Nielsen B.D, Schott H.C, Geor R, Yokoyama M, Harris P. Effects of fiber type, particle size, and soak time on water hydration and estimated potential water releasing capacities of roughages fed to horses.. J. Equine Vet. Sci. 2011;31:261.
    doi: 10.1016/j.jevs.2011.03.068google scholar: lookup
  44. Argenzio R.A, Southworth M, Lowe J.E, Stevens C.E. Interrelationship of Na, HCO3, and volatile fatty acid transport by equine large intestine.. Am. J. Physiol. 1977;233:469–478.
    doi: 10.1152/ajpendo.1977.233.6.E469pubmed: 596440google scholar: lookup
  45. Clarke L.L, Argenzio R.A, Roberts M.C. Effect of meal feeding on plasma volume and urinary electrolyte clearance in ponies.. Am. J. Vet. Res. 1990;51:571–576.
    pubmed: 2327617
  46. Nyman S. Water Intake and Fluid Regulation in the Horse.. Ph.D. Thesis. Acta Universitatis Agriculturae Sueciae Veterinaria 98; Uppsala, Sweden: 2001.
  47. Houpt T.R. Water and electrolytes.. In: Swenson M.J., Reece W.O., editors. Dukes’ Physiology of Domestic Animals. 11th ed. Cornell University Press; Ithaca, NY, USA: 1993. pp. 9–21.
  48. Fonnesbeck P.V. Consumption and excretion of water by horses receiving all hay and hay-grain diets.. J. Anim. Sci. 1968;27:1350–1356.
    doi: 10.2527/jas1968.2751350xgoogle scholar: lookup
  49. Muhonen S, Lindberg J.E, Bertilsson J, Jansson A. Effects on fluid balance, digestion and exercise response in Standardbred horses fed silage, haylage and hay.. Comp. Exerc. Physiol. 2009;5:133–142.
    doi: 10.1017/S1478061509342334google scholar: lookup
  50. Andersson B.E, Jónasson H. Temperature regulation and environmental physiology.. In: Swenson M.J., Reece W.O., editors. Dukes’ Physiology of Domestic Animals. 11th ed. Cornell University Press; Ithaca, NY, USA: 1993. pp. 886–895.
  51. Meyer R.M, Bartley E.E. Boat in cattle. XV. The relation of viscosity and cell-free polysaccharide content of rumen fluid to feedlot bloat.. J. Anim. Sci. 1971;33:1018–1021.
    doi: 10.2527/jas1971.3351018xpubmed: 5165942google scholar: lookup
  52. Takahashi T, Sakata T. Large particles increase viscosity and yield stress of pig cecal contents without changing basic viscoelastic properties.. J. Nutr. 2002;132:1026–1030.
    doi: 10.1093/jn/132.5.1026pubmed: 11983832google scholar: lookup
  53. McRorie J, Brown S, Cooper R, Givaruangsawat S, Scruggs D, Boring G. Effects of dietary fibre and olestra on regional apparent viscosity and water content of digesta residue in porcine large intestine.. Aliment. Pharmacol. Ther. 2000;14:471–477.
    doi: 10.1046/j.1365-2036.2000.00734.xpubmed: 10759627google scholar: lookup
  54. Dikeman C.L, Barry K.A, Murphy M.R, Fahey G.C. Jr. Diet and measurement techniques affect small intestinal digesta viscosity among dogs.. Nutr. Res. 2007;27:56–65.
    doi: 10.1016/j.nutres.2006.12.005google scholar: lookup
  55. Jensen R.B, Austbø D, Blache D, Bach Knudsen K.E, Tauson A.H. The effect of feeding barley or hay alone or in combination with molassed sugar beet pulp on the metabolic responses in plasma and caecum of horses.. Anim. Feed Sci. Technol. 2016;214:53–65.
    doi: 10.1016/j.anifeedsci.2016.02.003google scholar: lookup
  56. Hansen T.L, Rankins E.M, Bobel J.M, McKinney M, Hackmann T.J, Warren L.K. Postprandial blood glucose and insulin responses of horses to feeds differing in soluble fiber concentration.. J. Equine Vet. Sci. 2020;88:102963.
    doi: 10.1016/j.jevs.2020.102963pubmed: 32303311google scholar: lookup

Citations

This article has been cited 2 times.
  1. Jackson MI, Wernimont SM, Carnagey K, Jewell DE. Nutrient Digestive Bypass: Determinants and Associations with Stool Quality in Cats and Dogs. Animals (Basel) 2024 Sep 26;14(19).
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  2. Muhonen S, Julliand V. Fibre Composition and Maturity of Forage-Based Diets Affects the Fluid Balance, Faecal Water-Holding Capacity and Microbial Ecosystem in French Trotters. Animals (Basel) 2023 Jan 17;13(3).
    doi: 10.3390/ani13030328pubmed: 36766217google scholar: lookup
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