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Home / Equine Research Bank / BMC Vet Res / Epithelial expression of mRNA and protein for IL-6, IL-10 an...
BMC veterinary research2008; 4; 8; doi: 10.1186/1746-6148-4-8

Epithelial expression of mRNA and protein for IL-6, IL-10 and TNF-alpha in endobronchial biopsies in horses with recurrent airway obstruction.

Abstract: The aim of this study was to evaluate the contribution of bronchial epithelium to airway inflammation, with focus on mRNA and protein expression of cytokines of innate immunity IL-6, IL-10 and TNF-alpha, in horses with Recurrent Airway Obstruction (RAO) during exacerbation and in remission. Results: Despite marked clinical and physiologic alterations between exacerbation and after remission in the RAO horses no differences were detected in either cytokine mRNA or protein levels. Moreover, the expression of investigated cytokines in RAO horses on pasture did not differ from controls. In comparing real-time PCR analysis to results of immunohistochemistry only IL-10 mRNA and protein levels in RAO horses on pasture were significantly correlated (rs = 0.893, p = 0.007). Curiously, in controls examined on pasture the TNF-alpha protein level was positively correlated to IL-10 mRNA expression (rs = 0.967, p = 0.007) and negatively correlated to IL-6 mRNA expression (rs = -0.971, p = 0.001). Conclusions: Given the complementary relationship of assessing cytokines directly by immunohistochemistry, or indirectly by PCR to mRNA, the lack of significant changes in either mRNA or protein levels of IL-6, IL-10 or TNF-alpha mRNA in RAO horses in exacerbation suggests that these particular cytokines in bronchial tissue may not play a substantive role in the active inflammation of this disease. To support this contention further studies examining time dependency of expression of IL-6, IL-10 or TNF-alpha are needed, as is expansion of the range of cytokines to include other key regulators of airway inflammation.
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Publication Date: 2008-02-23 PubMed ID: 18294392PubMed Central: PMC2294109DOI: 10.1186/1746-6148-4-8Google Scholar: Lookup
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  • Journal Article
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article investigates the role of specific cytokines in the bronchial inflammation associated with Recurrent Airway Obstruction (RAO) in horses. The results show that these cytokines may not play a significant role in active inflammation during RAO exacerbation.

Objective of the Study

  • The researchers aimed to analyze the role of bronchial epithelium in airway inflammation during RAO in horses. They focused specifically on mRNA and protein expression of immune system cytokines IL-6, IL-10 and TNF-alpha during both periods of disease exacerbation and remission.

Exacerbation and Remission of RAO in Horses

  • Despite noticing clinical and physiological changes between periods of RAO exacerbation and remission, the researchers found no differences in cytokine mRNA or protein levels in these horses.
  • It was also observed that the cytokine expression in RAO horses on pasture was similar to control horses not suffering from RAO.

Correlation Analysis

  • When comparing the results of cytokine analysis using real-time PCR (polymerase chain reaction) and immunohistochemistry, they found only one significant correlation in RAO horses on pasture. The IL-10 mRNA and protein levels showed a significant positive correlation.
  • In control horses on pasture, the protein level of TNF-alpha was found to be positively correlated to IL-10 mRNA expression, and negatively correlated to IL-6 mRNA expression.

Conclusions

  • The research suggests that the lack of substantial changes in mRNA or protein levels of IL-6, IL-10, or TNF-alpha in RAO horses during exacerbation implies that these specific cytokines may not play a significant role in inflammation associated with this disease.
  • To confirm this hypothesis, the researchers suggest further research is necessary to study the time-dependent expression of these cytokines, and possibly expanding the study to include other cytokines that may regulate airway inflammation.

Cite This Article

APA
Riihimäki M, Raine A, Pourazar J, Sandström T, Art T, Lekeux P, Couëtil L, Pringle J. (2008). Epithelial expression of mRNA and protein for IL-6, IL-10 and TNF-alpha in endobronchial biopsies in horses with recurrent airway obstruction. BMC Vet Res, 4, 8. https://doi.org/10.1186/1746-6148-4-8

Publication

BMC veterinary research
ISSN: 1746-6148
NlmUniqueID: 101249759
Country: England
Language: English
Volume: 4
Pages: 8

Researcher Affiliations

Riihimäki, Miia
  • Department of Clinical Sciences, Equine Internal Medicine, Swedish University of Agricultural Sciences, Uppsala, Sweden. Miia.Riihimaki@kv.slu.se
Raine, Amanda
    Pourazar, Jamshid
      Sandström, Thomas
        Art, Tatiana
          Lekeux, Pierre
            Couëtil, Laurent
              Pringle, John

                MeSH Terms

                • Animals
                • Biopsy / veterinary
                • Bronchi / cytology
                • Bronchi / metabolism
                • Epithelial Cells / metabolism
                • Gene Expression Regulation
                • Horse Diseases / genetics
                • Horse Diseases / metabolism
                • Horses
                • Interleukin-1 / genetics
                • Interleukin-1 / metabolism
                • Interleukin-10 / genetics
                • Interleukin-10 / metabolism
                • Lung Diseases, Obstructive / genetics
                • Lung Diseases, Obstructive / metabolism
                • Lung Diseases, Obstructive / veterinary
                • RNA, Messenger / genetics
                • RNA, Messenger / metabolism
                • Respiratory Mucosa / cytology
                • Respiratory Mucosa / metabolism
                • Tumor Necrosis Factor-alpha / genetics
                • Tumor Necrosis Factor-alpha / metabolism

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                Citations

                This article has been cited 7 times.
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