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Home / Equine Research Bank / Equine Vet J / Equine bone marrow-derived mesenchymal stromal cells (BMDMSC...
Equine veterinary journal2012; 45(3); 372-375; doi: 10.1111/j.2042-3306.2012.00646.x

Equine bone marrow-derived mesenchymal stromal cells (BMDMSCs) from the ilium and sternum: are there differences?

Abstract: The 2 sites of bone marrow harvest for isolation of mesenchymal stromal cells (MSC) in the horse are the sternum and ilium. The technical procedure is based on practitioner preference, but no studies have compared MSC concentrations and growth rates between the sites in horses aged 2-5 years. Objective: The objective of this study was to compare nucleated cell counts and growth rates between the sternum and ilium and between consecutive 5 ml bone marrow aspirates. We hypothesised that there would be a higher concentration of MSCs in the sternum than the ilium, and that the first sequential aspirate from either site would yield the greatest concentration of MSCs. We hypothesised that growth rates of cells from each site would not differ. Methods: Seven horses, aged 2 to 5 years, had 2 sequential 5 ml marrow aspirates taken from the sternum and ilium. Nucleated cell counts (NCCs) were obtained before and after marrow processing. Cells were expanded in culture for 3 passages and growth rate characteristics compared for all aspirates. Results: The NCCs of the first 5 ml aspirate were higher than those of the second 5 ml aspirate for both sites (P0.05). Conclusions: The NCCs and growth rates of progenitor cells in the ilium and sternum are similar for horses in the 2-5 year age category. The first 5 ml bone marrow aspirate has a higher concentration of NCCs and resulting bone marrow-derived MSC population than subsequent aspirates. Conclusions: The first 5 ml aspirates from the sternum and ilium offer a rich supply of bone marrow-derived MSCs with similar growth rate characteristics. The harvesting procedure of only a 5 ml draw from either the sternum or ilium should result in adequate numbers of MSCs.
© 2012 EVJ Ltd.
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Publication Date: 2012-09-26 PubMed ID: 23009322PubMed Central: PMC3581704DOI: 10.1111/j.2042-3306.2012.00646.xGoogle Scholar: Lookup
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  • Extramural

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper studied the differences between Equine bone marrow-derived mesenchymal stromal cells (BMDMSCs) sourced from both the ilium and sternum of horses aged 2-5 years. The key findings were that nucleated cell counts and growth rates were similar in BMDMSCs from both areas, and the first 5 ml bone marrow aspirate offered a higher concentration of BMDMSCs compared to subsequent ones.

Objective of the Research

  • The objective of this research was to explore the differences, if any, between the BMDMSCs collected from the sternum and the ilium of horses, with a specific focus on nucleated cell counts (NCCs) and growth rates.
  • The hypothesis was that the sternum would have a higher concentration of MSCs compared to the ilium and that the first sequential aspirate would yield the highest concentration of MSCs from either site. The study also hypothesized that there wouldn’t be any discernible differences in the growth rates of cells collected from the two sites.

Methods

  • The study involved seven horses aged between 2-5 years, from which two sequential 5ml marrow aspirates were taken from both the sternum and ilium. The NCCs were measured before and after marrow processing.
  • The cells were cultured for three passages and the growth rate characteristics of each aspirate sample were compared.

Results

  • Results revealed that the NCCs of the initial 5ml aspirate were higher than those of the second 5ml aspirate from both the sternum and ilium.The growth rates did not vary significantly across all the groups.

Conclusions

  • Based on the study’s findings, both the sternum and ilium provide a similar supply of nucleated cells and growth rates for horses aged between 2 to 5 years. The first 5ml bone marrow aspirate provides a higher concentration of NCCs and MSC populations compared to any subsequent aspirates.
  • In conclusion, the study established that the first 5ml aspirates from both the sternum and ilium provide an ample supply of BMDMSCs with comparable growth rates. Therefore, even a single 5ml draw from either the sternum or ilium should provide sufficient MSCs for study or application.

Cite This Article

APA
Adams MK, Goodrich LR, Rao S, Olea-Popelka F, Phillips N, Kisiday JD, McIlwraith CW. (2012). Equine bone marrow-derived mesenchymal stromal cells (BMDMSCs) from the ilium and sternum: are there differences? Equine Vet J, 45(3), 372-375. https://doi.org/10.1111/j.2042-3306.2012.00646.x

Publication

Equine veterinary journal
ISSN: 2042-3306
NlmUniqueID: 0173320
Country: United States
Language: English
Volume: 45
Issue: 3
Pages: 372-375

Researcher Affiliations

Adams, M K
  • Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado, USA.
Goodrich, L R
    Rao, S
      Olea-Popelka, F
        Phillips, N
          Kisiday, J D
            McIlwraith, C W

              MeSH Terms

              • Animals
              • Bone Marrow Cells / cytology
              • Female
              • Horses
              • Ilium
              • Male
              • Mesenchymal Stem Cells / cytology
              • Sternum

              Grant Funding

              • K08 AR054903 / NIAMS NIH HHS
              • 1K08AR054903-01A2 / NIAMS NIH HHS

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              Citations

              This article has been cited 9 times.
              1. Looney AM, Leider JD, Horn AR, Bodendorfer BM. Bioaugmentation in the surgical treatment of anterior cruciate ligament injuries: A review of current concepts and emerging techniques.. SAGE Open Med 2020;8:2050312120921057.
                doi: 10.1177/2050312120921057pubmed: 32435488google scholar: lookup
              2. Bogers SH. Cell-Based Therapies for Joint Disease in Veterinary Medicine: What We Have Learned and What We Need to Know.. Front Vet Sci 2018;5:70.
                doi: 10.3389/fvets.2018.00070pubmed: 29713634google scholar: lookup
              3. Eydt C, Geburek F, Schröck C, Hambruch N, Rohn K, Pfarrer C, Staszyk C. Sternal bone marrow derived equine multipotent mesenchymal stromal cells (MSCs): investigations considering the sampling site and the use of different culture media.. Vet Med Sci 2016 Aug;2(3):200-210.
                doi: 10.1002/vms3.36pubmed: 29067195google scholar: lookup
              4. Ghem C, Dias LD, Sant'Anna RT, Kalil RAK, Markoski M, Nardi NB. Combined Analysis of Endothelial, Hematopoietic, and Mesenchymal Stem Cell Compartments Shows Simultaneous but Independent Effects of Age and Heart Disease.. Stem Cells Int 2017;2017:5237634.
                doi: 10.1155/2017/5237634pubmed: 28819363google scholar: lookup
              5. Dias LD, Casali KR, Ghem C, da Silva MK, Sausen G, Palma PB, Covas DT, Kalil RAK, Schaan BD, Nardi NB, Markoski MM. Mesenchymal stem cells from sternum: the type of heart disease, ischemic or valvular, does not influence the cell culture establishment and growth kinetics.. J Transl Med 2017 Jul 25;15(1):161.
                doi: 10.1186/s12967-017-1262-0pubmed: 28743269google scholar: lookup
              6. Metcalf GL, McClure SR, Hostetter JM, Martinez RF, Wang C. Evaluation of adipose-derived stromal vascular fraction from the lateral tailhead, inguinal region, and mesentery of horses.. Can J Vet Res 2016 Oct;80(4):294-301.
                pubmed: 27733784
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