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Home / Equine Research Bank / Animals (Basel) / Equine Corneal Wound Healing Using Mesenchymal Stem Cell Sec...
Animals : an open access journal from MDPI2024; 14(13); doi: 10.3390/ani14131842

Equine Corneal Wound Healing Using Mesenchymal Stem Cell Secretome: Case Report.

Abstract: Corneal ulcers are a common and potentially vision-threatening condition in horses that can be challenging to treat with conventional therapies alone. This case report describes the successful treatment of a non-healing corneal ulcer in a 28-year-old Hispano-Bretón mare using the secretome derived from adipose tissue-derived mesenchymal stem cells (ASCs). Despite initial treatment with antibiotics, anti-inflammatory drugs, and surgical debridement, the corneal ulcer failed to heal properly, exhibiting persistent epithelial defects and stromal complications. As an alternative regenerative approach, the ASC secretome, a rich source of trophic factors, cytokines, and extracellular vesicles, was topically administered to the affected eye. Remarkably, within one week of secretome treatment, the clinical signs of blepharospasm and epiphora resolved, and the corneal ulcer exhibited complete re-epithelialization, regained transparency, and reduced neovascularization. No recurrence was observed during the 1.5-year follow-up period. This case highlights the potential therapeutic benefits of the ASC secretome in promoting corneal wound healing and suggests its promise as a novel cell-free therapy for treating refractory corneal ulcers in horses.
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Publication Date: 2024-06-21 PubMed ID: 38997954PubMed Central: PMC11240377DOI: 10.3390/ani14131842Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research presents a case where a previously untreatable corneal ulcer in a horse was successfully treated with a substance produced by mesenchymal stem cells from adipose tissue, which showed promising implications for improving corneal wound healing in horses.

Objective of the Research

  • The main goal of this study was to evaluate the efficacy of using the secretome from adipose tissue-derived mesenchymal stem cells (ASCs) to treat corneal ulcers in horses which have proved resistant to conventional treatments.

Research Methodology

  • The mare was initially treated with traditional therapies involving antibiotics, anti-inflammatory drugs, and surgical debridement but showed no signs of healing, having persistent epithelial defects and stromal complications.
  • As an alternative therapy, the researchers opted to use the ASC secretome, administered topically to the afflicted area of the eye. The secretome is a combination of growth factors, cytokines, and extracellular vesicles, products of the ASCs.

Findings and Conclusion

  • In a span of one week after initiating the treatment with the ASC secretome, the horse recuperated significantly, with visible signs of corneal re-epithelialization, regain of transparency, decrease in neovascularization, and resolution of clinical signs such as blepharospasm and epiphora.
  • No recurrence of the ulcer was encountered during the follow-up period of 1.5 years, indicating the effectiveness and potential benefits of the ASC secretome in fostering corneal wound healing.
  • The positive outcome from this case study points out the potential of ASC secretome as a novel, non-cellular therapy for the treatment of stubborn corneal ulcers in horses. This method may present promising implications for veterinary medicine and equine health care in the future.

Cite This Article

APA
Casado-Santos A, González-Cubero E, González-Fernández ML, González-Rodríguez Y, García-Rodríguez MB, Villar-Suárez V. (2024). Equine Corneal Wound Healing Using Mesenchymal Stem Cell Secretome: Case Report. Animals (Basel), 14(13). https://doi.org/10.3390/ani14131842

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 14
Issue: 13

Researcher Affiliations

Casado-Santos, Alejandro
  • Department of Surgery, Medicine and Veterinary Anatomy, Faculty of Veterinary Sciences, Campus de Vegazana, University of Léon, 24071 Léon, Spain.
González-Cubero, Elsa
  • Department of Neurosurgery, Stanford School of Medicine, Stanford University, Palo Alto, CA 94305, USA.
González-Fernández, Maria Luisa
  • Department of Surgery, Medicine and Veterinary Anatomy, Faculty of Veterinary Sciences, Campus de Vegazana, University of Léon, 24071 Léon, Spain.
González-Rodríguez, Yaiza
  • Department of Surgery, Medicine and Veterinary Anatomy, Faculty of Veterinary Sciences, Campus de Vegazana, University of Léon, 24071 Léon, Spain.
García-Rodríguez, Mª Belén
  • Department of Surgery, Medicine and Veterinary Anatomy, Faculty of Veterinary Sciences, Campus de Vegazana, University of Léon, 24071 Léon, Spain.
Villar-Suárez, Vega
  • Department of Surgery, Medicine and Veterinary Anatomy, Faculty of Veterinary Sciences, Campus de Vegazana, University of Léon, 24071 Léon, Spain.
  • Institute of Biomedicine (IBIOMED), Faculty of Veterinary Sciences, Campus de Vegazana, University of León, 24071 León, Spain.

Conflict of Interest Statement

The authors declare no conflicts of interest.

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