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Methods and protocols2022; 5(3); 51; doi: 10.3390/mps5030051

Equine Oviductal Organoid Generation and Cryopreservation.

Thompson, RE
·
Meyers, MA
·
Veeramachaneni, DNR
·
Pukazhenthi, BS
·
Hollinshead, FK

Abstract: Organoids are a type of three-dimensional (3D) cell culture that more closely mimic the in vivo environment and can be maintained in the long term. To date, oviductal organoids have only been reported in laboratory mice, women, and cattle. Equine oviductal organoids were generated and cultured for 42 days (including 3 passages and freeze-thawing at passage 1). Consistent with the reports in mouse and human oviductal organoids, the equine oviductal organoids revealed round cell clusters with a central lumen. Developing a 3D model of the mare oviduct may allow for an increased understanding of their normal physiology, including hormonal regulation. These organoids may provide an environment that mimics the in vivo equine oviduct and facilitate improved in vitro embryo production in equids.

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Publication Date: 2022-06-15 PubMed ID: 35736552PubMed Central: PMC9230449DOI: 10.3390/mps5030051Google Scholar: Lookup

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This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research is about the successful creation and cultivation of three-dimensional cell structures, called organoids, from the oviducts of horses. This development provides a model to better understand the normal physiology of horse oviducts and could improve in-vitro embryo production in horse breeds.

Creating Oviductal Organoids in Horses

This research involved generating and cultivating organoids from the oviducts of horses, which are miniature, self-organizing versions of organs. Organoids are 3D structures grown from stem cells that mimic the behaviors and functions of the organ they are derived from.
In the past, such organoids have been successfully generated from laboratory mice, women, and cattle, making this the first time they have been developed from horses. This allows researchers to study in detail the cell behaviors within the horse’s oviduct within a controlled lab environment.

Growth and Preservation of the Organoids

The horse oviduct organoids were cultured for 42 days, during which they were passaged three times and freeze-thawed at one passage. The term “passaging” refers to the process of transferring cells from the original culture into a new one to allow for further growth or study.
During this period, the organoids developed into round cell clusters with a central lumen, similar to what has been reported in mice and human oviductal organoids. This consistency suggests the successful growth and preservation of the organoids over the 42-day period.

Potential Applications of the Study

Developing these organoids can lead to a better understanding of the physiology of mares, including how they are impacted by hormonal regulation. Knowing this information is crucial in the study of equine reproduction and health.
Furthermore, providing an environment that simulates the horse’s oviduct can improve the successful production of embryos in-vitro. This can promote better breeding methods for horses and potentially increase the sustainable production of specific horse breeds.

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APA

Thompson RE, Meyers MA, Veeramachaneni DNR, Pukazhenthi BS, Hollinshead FK. (2022). Equine Oviductal Organoid Generation and Cryopreservation. Methods Protoc, 5(3), 51. https://doi.org/10.3390/mps5030051

Publication

Methods and protocols

ISSN: 2409-9279

NlmUniqueID: 101720073

Country: Switzerland

Language: English

Volume: 5

Issue: 3

PII: 51

Researcher Affiliations

Thompson, Riley E

Department of Clinical Sciences, Colorado State University, Fort Collins, CO 80523, USA.

Meyers, Mindy A

Department of Clinical Sciences, Colorado State University, Fort Collins, CO 80523, USA.

Veeramachaneni, D N Rao

Department of Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.

Pukazhenthi, Budhan S

Center for Species Survival, Smithsonian National Zoo and Conservation Biology Institute, Front Royal, VA 22630, USA.

Hollinshead, Fiona K

Department of Clinical Sciences, Colorado State University, Fort Collins, CO 80523, USA.

Grant Funding

N/A / Colorado State University Research Council of the College of Veterinary Medicine and Biomedi-cal Sciences

Conflict of Interest Statement

The authors declare no conflict of interest.

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Citations

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