English
French
Methods and protocols.
Publisher:
MDPI AG,
Frequency: Quarterly
Country: Switzerland
Language: English
Start Year:2017 -
ISSN:
2409-9279 (Electronic)
2409-9279 (Linking)
2409-9279 (Linking)
Impact Factor
2.4
| NLM ID: | 101720073 |
| (OCoLC): | 1048115922 |
| LCCN: | 2019243059 |
| Classification: | W1 |
Optimization of the Amplification of Equine Muscle-Derived Mesenchymal Stromal Cells in a Hollow-Fiber Bioreactor. The main causes of mortality in horses are the gastrointestinal pathologies associated with septic shock. Stem cells have shown, through systemic injection, a capacity to decrease inflammation and to regenerate injured tissue faster. Nevertheless, to achieve this rapid and total regeneration, systemic injections of 1 to 2 million cells per kilogram of body weight must be considered. Here, we demonstrate for the first time the feasibility and expansion capacity of equine muscle-derived mesenchymal stromal cells (mdMSCs) in a functionally closed, automated, perfusion-based, hollow-fiber bioreact...
Equine Oviductal Organoid Generation and Cryopreservation. Organoids are a type of three-dimensional (3D) cell culture that more closely mimic the in vivo environment and can be maintained in the long term. To date, oviductal organoids have only been reported in laboratory mice, women, and cattle. Equine oviductal organoids were generated and cultured for 42 days (including 3 passages and freeze-thawing at passage 1). Consistent with the reports in mouse and human oviductal organoids, the equine oviductal organoids revealed round cell clusters with a central lumen. Developing a 3D model of the mare oviduct may allow for an increased understanding of t...
