Equine peripheral blood-derived progenitors in comparison to bone marrow-derived mesenchymal stem cells.
Abstract: Fibroblast-like cells isolated from peripheral blood of human, canine, guinea pig, and rat have been demonstrated to possess the capacity to differentiate into several mesenchymal lineages. The aim of this work was to investigate the possibility of isolating pluripotent precursor cells from equine peripheral blood and compare them with equine bone marrow-derived mesenchymal stem cells. Human mesenchymal stem cells (MSCs) were used as a control for cell multipotency assessment. Venous blood (n = 33) and bone marrow (n = 5) were obtained from adult horses. Mononuclear cells were obtained by Ficoll gradient centrifugation and cultured in monolayer, and adherent fibroblast-like cells were tested for their differentiation potential. Chondrogenic differentiation was performed in serum-free medium in pellet cultures as a three-dimensional model, whereas osteogenic and adipogenic differentiation were induced in monolayer culture. Evidence for differentiation was made via biochemical, histological, and reverse transcription-polymerase chain reaction evaluations. Fibroblast-like cells were observed on day 10 in 12 out of 33 samples and were allowed to proliferate until confluence. Equine peripheral blood-derived cells had osteogenic and adipogenic differentiation capacities comparable to cells derived from bone marrow. Both cell types showed a limited capacity to produce lipid droplets compared to human MSCs. This result may be due to the assay conditions, which are established for human MSCs from bone marrow and may not be optimal for equine progenitor cells. Bone marrow-derived equine and human MSCs could be induced to develop cartilage, whereas equine peripheral blood progenitors did not show any capacity to produce cartilage at the histological level. In conclusion, equine peripheral blood-derived fibroblast-like cells can differentiate into distinct mesenchymal lineages but have less multipotency than bone marrow-derived MSCs under the conditions used in this study.
Publication Date: 2006-06-14 PubMed ID: 16769763DOI: 10.1634/stemcells.2005-0264Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The study aims to determine the ability of precursor cells isolated from horse peripheral blood to differentiate into different cell types and to compare this ability with that of horse bone marrow-derived stem cells. The results showed that these blood-derived cells have similar resiliency to become other cell types as those taken from bone marrow. However, they are less efficient at producing certain types of tissue, such as cartilage, under specific conditions.
Research Methodology
- The researchers collected venous blood and bone marrow samples from adult horses. They then isolated mononuclear cells from these samples using Ficoll gradient centrifugation, a technique used to separate different cell types based on their density.
- These cells were cultured in a monolayer culture, allowing the adherent fibroblast-like cells to be examined for their potential to differentiate into different cell types.
- Three different methods were used to cultivate different cell types: chondrogenic differentiation was carried out in serum-free medium as a three-dimensional model, and osteogenic and adipogenic differentiation were stimulated in monolayer culture.
- Cell differentiation was confirmed through biochemical, histological, and reverse transcription-polymerase chain reaction evaluations.
Research Findings
- Fibroblast-like cells were observed in 12 out of 33 samples by day 10, and these cells were allowed to grow until they covered the surface of the culture dish (reached confluence).
- Peripheral blood-derived cells demonstrated osteogenic and adipogenic differentiation capacities that were similar to those of bone marrow-derived cells.
- Both types of cells showed a limited ability to form lipid droplets compared to human mesenchymal stem cells, a phenomenon that could be attributed to the culture conditions, which were optimized for human cells.
- While both bone marrow-derived horse and human stem cells were able to form cartilage, peripheral blood progenitors from horses did not possess this ability.
Conclusion
- This study concluded that while horse peripheral blood-derived fibroblast-like cells can differentiate into distinct mesenchymal lineages (bone, fat), their versatility (or multipotency) is lower than that of bone marrow-derived stem cells, especially under the specific conditions used in this study.
Cite This Article
APA
Koerner J, Nesic D, Romero JD, Brehm W, Mainil-Varlet P, Grogan SP.
(2006).
Equine peripheral blood-derived progenitors in comparison to bone marrow-derived mesenchymal stem cells.
Stem Cells, 24(6), 1613-1619.
https://doi.org/10.1634/stemcells.2005-0264 Publication
Researcher Affiliations
- Institute of Pathology, Tissue Engineering Unit, University of Bern, Switzerland.
MeSH Terms
- Adipogenesis
- Animals
- Base Sequence
- Cell Differentiation
- Cells, Cultured
- Chondrogenesis
- DNA, Complementary / genetics
- Gene Expression
- Hematopoietic Stem Cells / cytology
- Hematopoietic Stem Cells / metabolism
- Horses / anatomy & histology
- Horses / blood
- Horses / metabolism
- Humans
- In Vitro Techniques
- Mesenchymal Stem Cells / cytology
- Mesenchymal Stem Cells / metabolism
- Multipotent Stem Cells / cytology
- Multipotent Stem Cells / metabolism
- Osteogenesis
- Pluripotent Stem Cells / cytology
- Pluripotent Stem Cells / metabolism
- Species Specificity
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